SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
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IF 2.88
Meng-Lei Xu,Yu Gao,Yali Li,Xueliang Li,Huanjie Zhang,Xiao Xia Han,Bing Zhao,Liang Su
Glyphosate is one of the most commonly-used and non-selective herbicides in agriculture, which may directly pollute the environment and threaten human health. A simple and effective approach to assessment of its damage to the natural environment is thus quite necessary. However, traditional chromatography-based detection methods usually suffer from complex pretreatment procedures. Herein, we propose a simple and sensitive method for the determination of glyphosate by combining ninhydrin reaction and surface-enhanced Raman scattering (SERS) spectroscopy. The product (purple color dye, PD) of the ninhydrin reaction is found to SERS-active and directly correlate with the glyphosate concentration. The limit of detection of the proposed method for glyphosate is as low as 1.43 × 10 − 8 mol·L − 1 with a relatively wider linear concentration range (1.0 × 10 − 7 -1.0 × 10 − 4 mol·L − 1 ), which demonstrates its great potential in rapid, highly sensitive concentration determination of glyphosate in practical applications for safety assessment of food and environment.
研究领域:
农业科学
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分析化学
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环境科学
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DOI
高影响因子
Metabolites
·
2023
·Vol. 13
·期 2
·283
·
IF 4.1
Haixing Li,Lingqin Wang,Lijuan Nie,Xiaohua Liu,Jinheng Fu
Gamma-aminobutyric acid (GABA) is a functional metabolite in various organisms. Herein, a sensitivity intensified ninhydrin-based chromogenic system (SINICS), achieved by ethanol and ethyl acetate, is described for the reliable relative quantitation of GABA. A 2.9 mL SINICS kit comprises 1% ninhydrin, 40% ethanol, 25% ethyl acetate, and 35 μL 0.2 M sodium acetate buffer (pH 5.0). In practice, following the addition of a 0.1 mL sample to the kit, the chromogenic reaction is completed by heating at 70 °C for 30 min. The kit increased the color development sensitivity of L-glutamic acid and GABA, with the detection limits being reduced from 20 mM and 200 mM to 5 mM and 20 mM, respectively. The chromophore was stable for at least 2 h at room temperature, which was sufficient for a routine colorimetric analysis. The absorbance at 570 nm with the deduction of background directly represents the content of amino acid. For a proof-of-concept, the SINICS was adopted to optimize the GABA fermentation process ofLevilactobacillus brevisCD0817. The results demonstrated that SINICS is an attractive alternative to the available ninhydrin-based colorimetric methods.Keywords:ninhydrin-based chromogenic system;colorimetry analysis;relative quantitation;gamma-aminobutyric acid;amino acids
研究领域:
生物化学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Nanomaterials
·
2020
·Vol. 10
·期 12
·2539
·
IF 4.324
Meng-Lei Xu,Yu Gao,Jing Jin,Jin-Feng Xiong,Xiao Xia Han,Bing Zhao
Glyphosate is one of the most commonly used and non-selective herbicides in agriculture, which may directly pollute the environment and threaten human health. A simple and effective approach to its detection is thus quite necessary. Surface-enhanced Raman scattering (SERS) spectroscopy was shown to be a very effective method to approach the problem. However, sensitivity in SERS experiments is quite low, caused by different orientation/conformation of the adsorbed molecules on the metal surface, which limit its detection by using SERS. In this paper, 2‒13C‒glyphosate (hereafter: 13–GLP) was designed as a model molecule for theoretical and experimental studies of the molecule structure. Vibrational modes were assigned based on the modeling results obtained at the B3LYP/6-311++G** level by density functional theory (DFT) calculations, which were performed to predict the FT‒IR and Raman spectra. Band downshifts were caused by13C atom isotopic substitution with mass changed. Moreover, SERS spectra of 13–GLP by combining ninhydrin reaction on Ag NPs were obtained. Isotopic Raman shifts are helpful in identifying the components of each Raman band through vibrations across the molecular system. They are coupled by probe molecules and thus bind to the substrates, indirectly offering the opportunity to promote interactions with Ag NPs and reduce the complex equilibrium between different orientation/conformation of glyphosate molecules on the metal surface.Keywords:glyphosate;isotopic Raman shifts;SERS;ninhydrin reaction
研究领域:
环境科学
·
分析化学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Haitao Xiong,Mengyang Wang,Ruirui Qiang,Yingchun Wu,Xingwang Zheng
Photodeposited TiO 2 /Ag nanocomposites were generally used to be a friendly catalyst for degrading organic contaminant in environmental field. However, electrochemiluminescence (ECL) sensing analysis based on photocatalysts remains a significant challenge. Herein, polyvinylimide (PEI)-TiO 2 /Ag nanocomposites (PEI-TiO 2 /AgNCPs) film with reduced graphene oxide(r-GO) was constructed as a sensing interface for copper(II) ECL detection. TiO 2 /Ag nanocomposites was prepared by reversed phase microemulsion method and photodeposition technique. Moreover, it was discovered that a small amount of Cu 2+ could obviously boost the ECL signal of ninhydrin-hydrogen peroxide system. Signal amplification was achieved by using the synergistic effect between r-GO and TiO 2 /Ag nanocomposites, and the efficiently concentrated effect of PEI to Cu 2+ . Furthermore, the investigation showed that ECL mechanism of ninhydrin-hydrogen peroxide system was attributed to the generated hydroxyl radical and superoxide anion during the several type of reactions. Thus for the first time, an ultrasensitive ECL approach for detecting Cu 2+ could be performed using ninhydrin as an ECL signal probe and hydrogen peroxide as a co-reaction reagent. Under the suitable circumstances, the proposed method showed an excellent linear relationship in the concentration range of Cu 2+ from 1.0 fM to 5.0 nM. Detection limit was estimated to be as low as 0.26 fM. The sensing interface expanded the application of photodeposited TiO 2 /Ag nanocomposites in ultrasensitive ECL detection. It has potential applications in other components and biological analysis.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Yingbi Chen,Jiali Tang,Shixing Wang,Libo Zhang
A chitosan-based adsorbents (CS-Ninhydrin) was prepared by grafting ninhydrin for Pb(II) ions adsorption. SEM-EDS, XRD and FTIR analysis were used to characterize the synthesized CS-Ninhydrin. The static adsorption experiments showed that CS-Ninhydrin had a good removal rate for Pb(II) ions in a wide range of pH 3 to 7, quickly reached equilibrium (120 min) and had a higher adsorption capacity (196 mg/g). Pseudo second-order and Langmuir models showed that the adsorption process of Pb(II) by CS-Ninhydrin was a single-layer chemical adsorption. Temperature experiments showed that the reaction was a spontaneous exothermic process. In the wastewater experiment, CS-Ninhydrin showed an excellent selectivity to Pb(II) ions. The reusability of CS-Ninhydrin was perfect after five adsorption-desorption cycles. The main adsorption mechanism was the chelating and electrostatic action between N and O groups in CS-Ninhydrin and Pb(II) ions. Therefore, the new adsorbent CS-Ninhydrin was expected to promote the wide application of chitosan in Pb(II) adsorption.
研究领域:
环境科学
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材料科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
FUEL
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2022
·
IF 8.035
Wenying Wang,Wei Wang,Zhongpeng Zhu,Xiaoming Hu,Fulin Qiao,Jing Yang,Dan Liu,Pu Chen,Qundan Zhang
The detergent additives added into gasoline can effectively reduce the accumulation of coke deposits in engine and improve the exhaust emission pollution. The cleaning effect of detergent additives depends on the contents and compositions of the active components which usually contain a terminal amino group. In this work, a quantitative method was established to determine the polyetheramines of detergent additives in gasoline by the ninhydrin reaction. Unlike the small molecule compounds containing amino groups, such as amino acids and alkylamines, the formation of vesicles significantly increased the reactivity between polyetheramines and ninhydrin. The ninhydrin reaction was prohibited when the vesicles of polyetheramines were destroyed by adding ethanol, which was confirmed by the mass spectrometry and dynamic light scattering results. At the optimized condition, the reaction between the polyetheramines and ninhydrin generated a blue-purple dye (Ruhemann’s purple) with a strong absorption at 560 nm. This reaction was highly selective, sensitive, and rapid. This quantitative method could distinguish the active components (polyetheramines) from the carrier oils (polyethers) in detergent additives. Aniline compounds in gasoline also did not interfere with the measurement of polyetheramines. The quantitative range was between 50 and 1000 mg/kg. The whole procedure could be finished in about 30 min. The accuracy of this method was verified using four different commercial polyetheramines and the results showed that the method was applicable to different types of detergent additives with relative errors lower than 10 %.
研究领域:
化学
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环境科学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
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IF 6
Shiyue Ma,Hongyu Chen,Xin Du,Lu Liu,Yanfeng Zhang,Rongliang Ma,Yongqiang Wen,Meiqin Zhang
Background The development of level 3 features in various latent fingerprints (LFPs) is pivotal to improving the matching accuracy of damaged fingerprints. Among the various LFP development methods, the traditional ninhydrin method is widely employed in forensic science. However, it exhibits two key limitations: first, it is only effective for developing eccrine LFPs; second, it can only reveal level 2 features. These constraints compromise the extraction efficiency and utility of the developed fingerprints, thereby restricting the method's applicable scenarios. Results We report the fabrication of core (polyvinyl alcohol ninhydrin (PVA-ninhydrin))-sheath (polyvinyl pyrrolidone (PVP)) fibrous films by using coaxial electrospinning technology for high-level development of various LFPs. The film achieves safe, fast and chemical visualization of eccrine, sebaceous and natural LFPs with levels 1–3 features, showing a better visualization effect than ninhydrin solution and PVA-Nin fibrous film. It may be attributed to high specific surface area of thin film with uniform distribution of ninhydrin in the PVA core for high proportion of active sites of amino acids, which enables the film to have strong sweat adsorption and spreading properties. It further allows a more thorough and complete reaction between ninhydrin and the amino acids in the fingerprint residues that enhances the contrast of the developed photos. The statistical analyses of sweat pore count, ridge width, and pore spacing show high consistency and accuracy. Significant This study developed the core (PVA-ninhydrin)-sheath (PVP) fibrous films to achieve safe, fast and chemical visualization of eccrine, sebaceous and natural LFPs with levels 1–3 features and accurate quantitative analysis of the developed eccrine one. Moreover, even the interspacing and angles between sweat pores can be derived from level 3 features of the developed fingerprints. These results lay a robust foundation for subsequent fingerprint identification and personal recognition.
研究领域:
法庭科学
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材料科学
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分析化学
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纳米技术
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生物识别
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DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2022
·Vol. 46
·期 40
·19442-19454
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IF 3.925
Zhi-Gang Yin,Xiong-Wei Liu,Lin Chen,Xiong-Li Liu,Bo-Wen Pan,Ying Zhou
A substrate-dependent, highly β-regioselective one-pot multicomponent 1,3-dipolar cycloaddition reaction of 3-methyl-4-nitro-5-isatylidenyl-isoxazoles with azomethine ylides (thermally generated in situ from ninhydrin and sarcosine) is reported for the first time, furnishing a library of biologically useful bispiro[oxindole-isoxazole-indandione] hybrids. Target novel and structurally complex compounds have been obtained in good yields and diastereoselectivities (up to 91% yield and 20 : 1 dr) with two adjacent spiro quaternary centres. Furthermore, the described method, which is different from our previous work of α-regioselective cycloaddition of 3-methyl-4-nitro-5-isatylidenyl-isoxazoles, is a highly β-regioselective cycloaddition. The newly synthesized compounds potently inhibited the proliferation of various cancer cell lines. The most potent compound 4ah could induce the apoptosis of K562 cells through a caspase-dependent and -independent pathway.
研究领域:
有机化学
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药物化学
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癌症研究
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）