BIORESOURCE TECHNOLOGY
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2020
·
IF 7.539
Junzhang Li,Zhaozhou Peng,Ruiyang Hu,Kaiyuan Gao,Chen Shen,Shouxin Liu,Runjing Liu
Accelerated denitrification is an essential problem in the biological treatment of nitrogenous wastewater. In this study, we report that denitrification is accelerated by micro-graphite particles (MGPs). The denitrification rate was increased by 83.4% or 11.1% in synthetic (with 0.16 g/L MGPs) or industrial nitrogenous wastewater (with 0.12 g/L MGP), respectively. The mechanism was revealed via a quantitative polymerase chain reaction (q-PCR), high-throughput sequencing, and scanning electron microscopy (SEM). The abundance of denitrifying bacteria Paracoccus in the sludge was increased by micro-graphite particles. The number of denitrifying bacteria with the nirS gene was increased significantly (75.6%). To the best of our knowledge, this is the first report that MGP could enhance denitrification via the sludge. MGP can denitrify in industrial applications.
研究领域:
环境生物学
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微生物学
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DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
·
2021
·Vol. 18
·期 7
·3633
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IF 3.39
Qianqian Lu,Nannan Zhang,Chen Chen,Miao Zhang,Dehua Zhao,Shuqing An
Lab-scale simulated biofilm reactors, including aerated reactors disturbed by short-term aeration interruption (AE-D) and non-aerated reactors disturbed by short-term aeration (AN-D), were established to study the stable-state (SS) formation and recovery after disturbance for nitrogen transformation in terms of dissolved oxygen (DO), removal efficiency (RE) of NH4+-N and NO3−-N and activity of key nitrogen-cycle functional genesamoA andnirS (RNA level abundance, per ball). SS formation and recovery of DO were completed in 0.56–7.75 h after transition between aeration (Ae) and aeration stop (As). In terms of pollutant REs, new temporary SS formation required 30.7–52.3 h after Ae and As interruptions, and seven-day Ae/As interruptions required 5.0% to 115.5% longer recovery times compared to one-day interruptions in AE-D and AN-D systems. According toamoA activity, 60.8 h were required in AE-D systems to establish new temporary SS after As interruptions, and RNAamoA copies (copy number/microliter) decreased 88.5%, while 287.2 h were required in AN-D systems, and RNAamoA copies (copy number/microliter) increased 36.4 times. FornirS activity, 75.2–85.8 h were required to establish new SSs after Ae and As interruptions. The results suggested that new temporary SS formation and recovery in terms of DO, pollutant REs andamoA andnirS gene activities could be modelled by logistic functions. It is concluded that temporary SS formation and recovery after Ae and As interruptions occurred at asynchronous rates in terms of DO, pollutant REs andamoA andnirS gene activities. Because of DO fluctuations, the quantitative relationship between gene activity and pollutant RE remains a challenge.Keywords:simulated biofilm reactor;stable state;nitrogen cycle;dissolved oxygen;gene activity
研究领域:
环境工程
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微生物学
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水处理
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2023
·
IF 9.8
Wantong Zhang,Xiuping Tao,Zhengyi Hu,Enze Kang,Zhongqing Yan,Xiaodong Zhang,Jinzhi Wang
Alpine peatlands are recognized as a weak or negligible source of nitrous oxide (N 2 O). Anthropogenic activities and climate change resulted in the altered water table (WT) levels and increased nitrogen (N) deposition, which could potentially transition this habitat into a N 2 O emission hotspot. However, the underlying mechanism related with the effects is still uncertain. Hence, we conducted a mesocosm experiment to address the response of growing-season N 2 O emissions and the gene abundances of nitrification (bacterial amoA ) and denitrification ( narG , nirS , norB and nosZ ) to the increased N deposition (20 kg N ha −1 yr −1 ) at two WT levels (WT − 30 , 30 cm below soil surface; WT 10 , 10 cm above soil surface) in the Zoige alpine peatland, Qinghai-Tibetan Plateau. The results showed that the WT did not affect N 2 O emissions, and this was attributed with the limitation of soil NO 3 − . The higher WT level increased denitrification ( narG and nirS gene abundance) resulting in the depletion of soil NO 3 − , but the consequent NO 3 − deficiency further limited denitrification, while the WT did not affect nitrification (bacterial amoA gene abundance). Meanwhile, the N deposition increased N 2 O emissions, regardless of WT levels. This was associated with the N-deposition induced increase in denitrification-related gene abundances of narG , nirS , norB and nosZ at WT − 30 and narG at WT 10 . Additionally, the N 2 O emission factor assigned to N deposition was 1.3 % at WT − 30 and 0.9 % at WT 10 , respectively. Our study provided comprehensive understanding of the mechanisms referring N 2 O emissions in response to the interactions between climate change and human disturbance from this high-altitude peatland.
研究领域:
环境科学
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生态学
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微生物生态学
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气候变化
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2019
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IF 5.108
Xingjia Yin,Jing Lu,Yuchun Wang,Guanglong Liu,Yumei Hua,Xiaoqiong Wan,Jianwei Zhao,Duanwei Zhu
Excessive nitrogen has been a global concern to cause lake eutrophication . The denitrification and anammox processes are considered to be effective biological pathways for nitrogen removal. Submerged macrophytes also play a key role in the nitrogen cycle of lakes. However, the mechanism of submerged macrophytes on regulating biological nitrogen removal pathways has not been well quantified. Therefore, this study investigated the impacts of submerged macrophytes on the community structures and abundance of the nirS -type denitrifiers and anammox bacteria in the rhizospheres. The qPCR results indicated that the abundance of two bacteria in the near-rhizospheres of submerged macrophytes was significantly lower than the root compartments and non-rhizospheres, while the concentrations of organic acids in the near-rhizospheres were higher than those of the root compartments and non-rhizospheres. Redundancy analysis results illustrated that concentrations of NO 3 − -N, NO 2 − -N, citric acid and oxalic acid were the key environmental indicators which had the significant impact on the microbial community . The concentrations of citric acid and oxalic acid were negatively correlated with the nirS -type denitrifiers abundance, and the oxalic acid concentrations were negatively correlated with the anammox bacteria abundance. These results indicated that submerged macrophytes could reduce the abundance of nirS -type denitrifiers and anammox bacteria by releasing organic acids. In addition, the highest diversity of denitrifier community were found in the rhizosphere of the Hydrilla verticillata , while the highest diversity of anammox community were found in the Potamogeton maackianus rhizosphere. These results indicate that the impacts of submerged macrophytes on the biological nitrogen removal pathways were species-dependent.
研究领域:
环境微生物学
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水生生态学
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生物地球化学
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DOI
PubMed
高影响因子
Plant Methods
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2021
·Vol. 17
·期 1
·1-14
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IF 4.993
Wang Maoyao,Li Xinru,Shen Yinjuan,Adnan Muhammad,Mao Le,Lu Pan,Hu Qian,Jiang Fuhong,Khan Muhammad Tahir,Deng Zuhu,Chen Baoshan,Huang Jiangfeng,Zhang Muqing
Background Sugarcane (Saccharum officinarum L.) is an economically important crop with stalks as the harvest organs. Improvement in stalk quality is deemed a promising strategy for enhancing sugarcane production. However, the lack of efficient approaches for systematic evaluation of sugarcane germplasm largely limits improvements in stalk quality. This study is designed to develop a systematic near-infrared spectroscopy (NIRS) assay for high-throughput phenotyping of sugarcane stalk quality, thereby providing a feasible solution for precise evaluation of sugarcane germplasm.Results A total of 628 sugarcane accessions harvested at different growth stages before and after maturity were employed to take a high-throughput assay to determine sugarcane stalk quality. Based on high-performance anion chromatography (HPAEC-PAD), large variations in sugarcane stalk quality were detected in terms of biomass composition and the corresponding fundamental ratios. Online and offline NIRS modeling strategies were applied for multiple purpose calibration with partial least square (PLS) regression analysis. Consequently, 25 equations were generated with excellent determination coefficients (R2) and ratio performance deviation (RPD) values. Notably, for some observations, RPD values as high as 6.3 were observed, which indicated their exceptional performance and predictive capability.Conclusion sThis study provides a feasible method for consistent and high-throughput assessment of stalk quality in terms of moisture, soluble sugar, insoluble residue and the corresponding fundamental ratios. The proposed method permits large-scale screening of optimal sugarcane germplasm for sugarcane stalk quality breeding and beyond.
研究领域:
农业
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植物科学
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光谱学
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生物技术
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
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2021
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IF 6.498
Zhi-Ling Li,Di Cao,Cai-Hua Bai,Xiao-Jing Yao,Xiao-Qiu Lin,Si-Pei Wang,Jun Nan,Bin Liang,Li-Wei Li,Kai Sun,Ai-Jie Wang
The response of the denitrification community to long-term antibiotic exposure requires further investigation. Here, the significantly altered denitrifying community structure and function were observed by continuous exposure to 1 mg/L sulfamethoxazole (SMZ) or chlortetracycline (CTC) for 180 d in the expanded granular sludge bed reactors. Thaurea , positively correlated with SMZ and NO 3 − removal efficiency ( NrE ), was highly enriched in the SMZ-added reactor, while, Comamons and Acinetobacter were largely inhibited. The acute inhibited and then gradual-recovered NrE (87.17–90.38 %) was observed with highly expressed narG , indicating the adaptability of Thaurea to SMZ. However, the abundance of Thaurea and Comamonas greatly decreased, while Melioribacter and Acinetobacter were largely enriched in the CTC-added reactor. CTC created more serious and continuous inhibition of NO 3 − reduction ( NrE of 64.53–66.95 %), with lowly expressed narG . Improved NO 2 − reduction capacity was observed in both reactors (70.16–95.42 %) with highly expressed nirS and nosZ , revealing the adaptability of NO 2 − reduction populations to antibiotics.
研究领域:
环境微生物学
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生物技术
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污水处理
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Xiaoqi Dong,Zidan Huang,Xingxing Peng,Xiaoshan Jia
A novel coupled yeast-sludge system (CYSS) was constructed by the yeast Candida sp. PNY integrated with activated sludge to treat non-sterile mainstream wastewater. After 240-day cultivation, compared with single activated sludge , simultaneous removal efficiency of total organic carbon (TOC), nitrogen and phosphorus increased by 19.5% (176.34 mg TOC g −1 d −1 ), 21.3% (11.25 mg TN g −1 d −1 ) and 15.0% (6.95 mg TP g −1 d −1 ), respectively, while the amount of sludge reduced by 50%. Amplicon sequencing analysis showed that the abundance of Nitrosomonas , Nitrospira , Zoogloea , Dechloromonas , and Candidatus Accumulibacter significantly decreased to 0% on Day 200. Abundance of nirS and nirK for denitrification significantly decreased in CYSS by quantitative PCR (qPCR), and the copies of nirS and nirK were 3.37-fold and 1.71-fold decrease from Day 0 to Day 240, respectively. The results of Fluorescence in situ hybridization and co-occurrence network showed that Candida sp. PNY predominated its distribution in CYSS, and strongly connected with environmental variables based on network analysis. Furthermore, this study reconstructed the carbon, nitrogen and phosphorus metabolic pathways of the CYSS based on metagenomics .
研究领域:
环境工程
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微生物学
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废水处理
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Yixing Zhang,Xueran Lin,Tang Xia,Hang Chen,Fu Huang,Chaohai Wei,Guanglei Qiu
The increased use of disinfection since the pandemic has led to increased effective chlorine concentration in municipal wastewater. Whereas, the specific impacts of active chlorine on nitrogen and phosphorus removal, the mediating communities, and the related metabolic activities in wastewater treatment plants (WWTPs) lack systematic investigation. We systematically analyzed the influences of chlorine disinfection on nitrogen and phosphorus removal activities using activated sludge from five full-scale WWTPs. Results showed that at an active chlorine concentration of 1.0 mg/g-SS, the nitrogen and phosphorus removal systems were not significantly affected. Major effects were observed at 5.0 mg/g-SS, where the nitrogen and phosphorus removal efficiency decreased by 38.9 % and 44.1 %, respectively. At an active chlorine concentration of 10.0 mg/g-SS, the nitrification, denitrification, phosphorus release and uptake activities decreased by 15.1 %, 69.5–95.9 %, 49.6 % and 100 %, respectively. The proportion of dead cells increased by 6.1 folds. Reverse transcriptional quantitative polymerase chain reaction (RT-qPCR) analysis showed remarkable inhibitions on transcriptions of the nitrite oxidoreductase gene ( nxr B), the nitrite reductase genes ( nir S and nir K), and the nitrite reductase genes ( nar G). The nitrogen and phosphorus removal activities completely disappeared with an active chlorine concentration of 25.0 mg/g-SS. Results also showed distinct sensitivities of different functional bacteria in the activated sludge. Even different species within the same functional group differ in their susceptibility. This study provides a reference for the understanding of the threshold active chlorine concentration values which may potentially affect biological nitrogen and phosphorus removal in full-scale WWTPs, which are expected to be beneficial for decision-making in WWTPs to counteract the potential impacts of increased active chlorine concentrations in the influent wastewater.
研究领域:
环境工程
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污水处理
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微生物学
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）