BIOSENSORS & BIOELECTRONICS
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2016
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IF 7.476
Xiangyu Jiang,Fenghua Geng,Yongxiang Wang,Jinhua Liu,Peng Qu,Maotian Xu
Quantitative fluorescence turn-on and colorimetric detection of glutathione (GSH) with rapid speed, low cost have attained much attention. Herein, we developed a sensitive fluorescence turn-on and colorimetric sensor for GSH based on the inner-filter effect (IFE), which is the first time to select oxTMB and TMPyP as the IFE absorber and fluorophore pair, respectively. The absorption band of oxTMB matches well with the emission band of TMPyP in the IFE-based fluorescent assay. In the absence of GSH, the absorption peak of oxTMB at 652 nm significantly overlaps with the emission of TMPyP, resulting in the efficient IFE and inhibition of the fluorescence of TMPyP. In the presence of GSH, the absorption intensity at 652 nm decreases, generating the recovery of the fluorescence of TMPyP. Therefore, this approach is demonstrated to be a novel candidate for detection of GSH, with high sensitivity and selectivity. The linear dynamic range for the concentrations of GSH is between 0.1 μM to 20 μM along with a limit of detection (LOD) of about 30 nM (calculated LOD as 3 σ /slope). Finally, this novel sensor was successfully applied for GSH detection in fetal calf serum , and satisfactory recovery was achieved.
研究领域:
分析化学
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生物传感
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医学检测
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 18
·4063-4071
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IF 3.286
Lin Shan,Zheng Danmin,Li Ailing,Chi Yuwu
It is well known that 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. The black oxTMB NWs showed very strong light absorption ability, thus could be used to greatly amplify the signal of Pt NP-based immunochromatography test strips (ICTSs). The Pt NP-based ICTSs with black oxTMB NW signal amplification have shown much better assay ability (linear response range and limit of detection) than those of gold nanoparticle (Au NP)-based ICTS, Pt NP-based ICTS, and Pt NP-based ICTS with blue or yellow oxTMB signal amplifications. The developed Pt NP-oxTMB NW-based ICTS has been demonstrated to be a new, accurate, sensitive, selective, and rapid immunosensor for quantitative detection of antigens such as human chorionic gonadotropin (HCG).
研究领域:
分析化学
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纳米技术
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生物传感器
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
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IF 2.5
Yuying Jia,Xiangyu Cai,Xuepeng Wang,Hongyan Shan,Yanfu Huan
Accurate detection of Cys/Hcy levels is critical for evaluating human health conditions. In this study, we developed an innovative fluorescence/colorimetric dual-mode sensing system to detect Cys/Hcy by dynamically adjusting the level of oxTMB. The system utilizes Au@PtNPs with peroxidase-like activity to catalyze the oxidation of colorless 3,3,5,5- tetramethylbenzidine (TMB) to blue oxTMB, and TAPB@DMTP COF, a fluorescent nanomaterial with abundant spatial pores, could be used as a carrier for oxTMB, and the two were made fluorescence quenching due to the fluorescence inner filter effect. The addition of Cys/Hcy allowed the originally dispersed Au@PtNPs to aggregate to inhibit their peroxidase-like activity, thus indirectly regulating the oxTMB content. At this point, the absorbance signal decreased and fluorescence was restored. To this end, we have developed a special fluorescence/colorimetric dual-mode sensing system for the detection of Cys/Hcy. The detection limits of the fluorescence and colorimetric methods for Cys/Hcy were determined to be 0.788 nM (Cys), 0.663 nM (Hcy), and 1.795 nM (Cys), 1.886 nM (Hcy), respectively. The sensing system was also evaluated for its ability to withstand interferences from 12 anions and 13 amino acids, demonstrating excellent unti-interference and selectivity. The recoveries of the two methods for detecting Cys/Hcy in fetal bovine serum were found to be 98.9%-114.3% with RSDs as low as 0.52% (fluorescence) and 0.92% (colorimetric), respectively.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
高影响因子
ANALYST
·
2020
·Vol. 146
·期 3
·956-963
·
IF 3.978
Jun Ma,Gege Feng,Ying Ying,Yong Shao,Yongxin She,Lufei Zheng,A. M. Abd EI-Aty,Jing Wang
Herein, an indirect SERS sensing assay was developed for the determination of glyphosate (Gly) in tap water. The mechanism of detection was based on relieving the inhibitory effect of L-cysteine (L-cys) on a Au–Pt nanozyme by combining Gly with L-cys through divalent copper ions (Cu2+). In this method, a novel nano-chain-like Au–Ag composite with good repeatability was successfully fabricated to detect SERS signals of oxTMB without disturbing TMB Raman signals. Under optimal conditions, the SERS signal intensity of oxTMB (at 1605 cm−1) was proportional to the concentration of Gly. The results showed a good linear response over the concentration ranges of 10 μg L−1 to 1000 mg L−1. The limit of detection and limit of quantitation of Gly were found to be 5 μg L−1 and 10 μg L−1, respectively. In addition, good anti-interference ability against interfering cations and structural analogues deserves to be mentioned. This SERS assay can be used for detection of Gly in tap water that can meet the needs of practical detection.
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Xin Yan,Chang Xia,Bin Chen,Yuan Fang Li,Peng Fei Gao,Cheng Zhi Huang
Plasmon resonance energy transfer (PRET), as a new form of energy transfer first discovered in 2007, has been widely applied for the biomolecular recognition, detection of ions, cellular physiological status monitoring, and energy conversion. It occurs between noble metal nanoparticles (donor) and conjugated molecules or nanoparticles (acceptor). In this study, we used urchin-like gold nanoplasmonics (UGPs) and oxTMB as a new donor–acceptor pair to establish a novel PRET coupling system, avoiding trivial modification. PRET from UGPs to conjugated redox-active oxTMB leads to resonant quenching in the localized surface plasmon resonance (LSPR) spectra. However, when the acid phosphatase (ACP) was introduced, the hydrolyzate ascorbic acid (AA) converted from 2-phospho-l-ascorbic acid trisodium salt (AAP) could be capable of reducing oxTMB into TMB, thereby preventing the occurrence of PRET. The recovery of the scattering spectral intensity of UGPs was linearly related to the concentration of ACP in the range of 0.1 to 5.0 U/L, and the ACP with a detection limit of 0.076 U/L could be measured. In addition, this method also showed good selectivity attributed to the substrate specificity of enzyme.
研究领域:
纳米技术
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
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