JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2024
·
IF 3.1
Xina Yu,Shanshan Song,Zhanhua Li,Tiantian Wang,Hui Huang,Qing Shen,Zongyuan Wu,Pei Luo
Panaxynol is a bioactive polyacetylene in food plants; however, its specific benefits in diabetes and metabolic disorders remain unclear. Previous studies have mainly focused on biochemical indicators and clinical evaluations. Limited research has systematically elucidated the beneficial effects of panaxynol from the oxylipins perspective. In this study, we employed an oxylipin analysis platform we previously established using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) based on the multiple reaction monitoring (MRM) method for the profiling of oxylipins. After a 7-week administration of panaxynol to db/db mice, significant alterations in serum oxylipins and potential benefits to hyperglycemia, insulin resistance, and hepatic steatosis were observed. Our analysis also revealed correlations among epoxygenase products derived from arachidonic acid (AA), linoleic acid (LA), and α-linolenic acid (ALA) via cytochrome P450 (CYP) pathways. Furthermore, six potential oxylipins were identified, as offering insights into the mechanisms by which panaxynol may modulate diabetes. These results provide the first in vivo evidence of the impact of panaxynol on oxylipin metabolism and lay the foundation for developing panaxynol as a nutraceutical for diabetes management.
研究领域:
代谢组学
·
营养学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Talanta Open
·
2026
·
IF 5.1
Di Rao,Zongyuan Wu,Zuojian Qin,Xia Xiang,Yu Chen,Dan Wang,Xin Lv,Tang Tang,Hong Chen,Fang Wei
Oxylipins, generated from polyunsaturated fatty acids (PUFAs), are essential for regulating fundamental physiological processes, such as tissue repair, immunity, and inflammation. However, their intracellular concentrations are inherently low, and many are rapidly transformed or degraded through enzymatic or non-enzymatic reactions, resulting in extremely short half-lives. The complex and time-consuming pretreatment, typically involving sequential cell lysis, solvent extraction, solid-phase clean-up, evaporation, and off-line chemical derivatization, undermines the accuracy of oxylipin analysis in cells. A high-throughput one-pot micro-volume chemical derivatization combined with targeted lipidomics analysis method was developed. Initially, cells were sorted into a 54-well micro sample tube array using flow cytometry. Then, a one-pot method was employed to simultaneously perform cell disruption, oxylipin extraction, and N, N‑diethyl-1,3-diaminopropane (DEPA) labeling. The derivatization of 54 cell samples was completed within 1 min at room temperature, enabling the entire pretreatment to be completed in 10 min. Finally, an ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was utilized for profiling oxylipins. A total of 14 oxylipins were identified in 1000 RAW 264.7 cell samples, and 8 distinct oxylipins were successfully detected in single-cell analysis including 12-HEPE, 15-HEPE, 12-HETE, PGD2, and PGE2. The proposed method exhibited high sensitivity, with concentration ranges of 0.03–2.98 nmol/L, excellent linearity (R² = 0.992–0.999), and a limit of detection as low as 0.2 pmol/L, demonstrating good accuracy and precision. This protocol eliminates solvent transfers, evaporation, and reconstitution through a one-step micro-volume reaction, drastically minimizing sample loss and oxidation risk. The entire workflow is completed in 10 min for 54 samples with -level sensitivity. Fully compatible with flow-sorted cells, automated liquid handling, and on-line MS, it delivers precise, reproducible oxylipin profiles from rare primary cells, clinical biopsies, or single cells, thereby opening new frontiers in single-cell lipidomics and precision medicine research.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）