INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2020
·Vol. 21
·期 6
·2211
·
IF 4.556
Wei Zhang,Danyang Li,Jianguang Zhang,Lingli Jiang,Zhaofa Li,Jun Sheng Lin
The compound 1,1,1-trichloro-2-(p-chlorophenyl)-2-(o-chlorophenyl) ethane (o,p’-DDT) has been identified as one of the endocrine-disrupting chemicals causing adverse effects on wildlife and even humans through bioaccumulation. Its detection has become increasingly important. We have obtained candidate aptamers binding too,p’-DDT by a systematic evolution of ligands by exponential enrichment (SELEX) protocol. Five out of seventeen candidate sequences were selected for preliminary characterization by SYBR Green I assay. One sequence with highest fluorescence response witho,p’-DDT, designated DDT_13, was chosen for further characterization. Its dissociation constant (Kd) was determined to be 412.3 ± 124.6 nM. DDT_13 exhibited low cross-binding activities on other tested small molecules. The good bioactivities of DDT_13 were demonstrated for the analysis of spiked lake water and tap water samples. This study provides a novelo,p’-DDT-specific probe for its future applications.Keywords:o,p’-DDT;aptamer;SELEX;AuNPs;biosensor;real water sample
研究领域:
环境科学
·
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Junyan Wei,Jianqiao Xu,Xinyuan Wei,Dongmei Zhou,Zhongbo Wei,Xiaosheng Tang,Qing Zheng,Zunyao Wang,Ruijuan Qu
Microplastics are widespread in aquatic environments and, due to their small size and similar density to water, often coexist with suspended particulate matter (SPM). Both microplastics and SPM act as carriers, playing key roles in the migration and transformation of organic contaminants. However, most studies have focused on isolated systems, necessitating an investigation into how co-existing microplastics affect the phototransformation behavior of hydrophobic pollutants on carrier surfaces. In this study, a model system was constructed with the most commonly detected polyethylene (PE) microplastics, dichlorodiphenyltrichloroethane (o,p’-DDT), and Yangtze River SPM. The results showed that the introduction of PE into the SPM system notably accelerated the photodegradation of o,p’-DDT, with a synergistic promotion between PE and SPM. Utilizing chemical probes, quenchers, and electrochemical experiments, we found that PE facilitated the electron transfer in SPM, thereby enhancing the generation of reactive oxygen species (ROS) and ultimately, leading to the synergistic effect. By combining liquid chromatography-mass spectrometry and gas chromatography-mass spectrometry, we identified 17 intermediates and proposed three main transformation pathways: elimination, hydroxylation, and dechlorination. The potential mechanisms by which ROS induced these reaction pathways were further validated by density functional theory calculations. Moreover, PE formed interaction products with o,p’-DDT. Initial environmental risk assessment using VEGA and EPI Suite software indicated the products became less toxic, but were more likely to remain in water. This study deepened the understanding of the photochemical effects of microplastics and provided new insights into the environmental fate and risk of organic pollutants.
研究领域:
环境科学
·
污染生态学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Dezhen Wang,Wentao Zhu,Yao Wang,Jin Yan,Miaomiao Teng,Jiyan Miao,Zhiqiang Zhou
Previous studies have demonstrated the endocrine disruption of o,p′-DDT. In this study, we used a 1H NMR based metabolomics approach to investigate the estrogenic effects of o,p′-DDT (300 mg/kg) on the uterus and brain after 3 days of oral gavage administration, and ethynylestradiol (EE, 100 μg/kg) was used as a positive control. A supervised statistical analysis (PLS-DA) indicated that o,p′-DDT exerted both estrogenic receptor-(ER)-dependent and independent effects on the uterus but mainly ER-independent effects on the brain at metabolome levels, which was verified by coexposing with the antiestrogenic ICI 182,780. Four changed metabolites—glycine, choline, fumarate, and phenylalanine—were identified as ER-independent alterations in the uterus, while more metabolites, including γ-aminobutyrate, N-acetyl aspartate, and some amino acids, were disturbed based on the ER-independent mechanism in the brain. Together with biological end points, metabolomics is a promising approach to study potential estrogenic chemicals.
研究领域:
毒理学
·
代谢组学
·
内分泌学
✓ 已验证
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2022
·
IF 5.572
Xiangyuan Liu,Li Song
Epidemiologic studies have revealed that Dichlorodiphenyltrichloroethane (DDT) and its metabolites are associated with liver diseases. However, there has been little emphasis on the mechanism underlying liver toxicity of o,p’- DDT and relevant effective inhibitors investigation. This study indicated o,p’- DDT exposure significantly decreased cell viability and promoted lactate dehydrogenase (LDH) release based on the investigation of cytotoxicity by trypan blue exclusion counts, MTT, and lactate dehydrogenase (LDH) assays. Comet, micronuclei, and DNA-protein crosslinks (DPC) assays demonstrated o,p’- DDT exposure increased the comet parameters, micronuclei frequency, and DPC coefficient. Meanwhile, we found o,p’- DDT induced mitochondria-dependent apoptosis , which is characterized by the loss of mitochondrial membrane potential (Δψm), decreased Bcl-2 expression, and increased protein levels of Bax, cytochrome c , activated-caspase-9, and activated-caspase-3. Furthermore, o,p’- DDT induced reactive oxygen species (ROS) overproduction, decreased the protein levels of nuclear factor erythroid-derived 2-like 2 (Nrf2) in the nuclear, and enhanced the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase . However, quercetin treatment significantly antagonized o,p’- DDT-induced cytotoxicity, genotoxicity , and apoptosis as well as effects on ROS, Nrf2, and NADPH oxidase. Taken together, these findings suggested quercetin could alleviate o,p’- DDT-induced toxicity in HL-7702 cells via inhibiting ROS production, which is modulated by down-regulating nuclear Nrf2 levels and NADPH oxidase expression.
研究领域:
毒理学
·
细胞生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Junyan Wei,Wenqian Cao,Hao Chen,Xiaosheng Tang,Zongli Huo,Feng Zhu,Zunyao Wang,Ruijuan Qu
The photoreactions on suspended particulate matter (SPM) surface were a non-negligible environmental transformation process for hydrophobic organic pollutants in river water environments. Herein, SPM samples from four representative basins in China—the Yangtze River, Yellow River, Pearl River, and Haihe River—were chosen and focused on the effects of differences in the SPM composition on the phototransformation behavior of 2,4′-dichlorodiphenyl trichloroethane (o,p′-DDT). The experimental results demonstrated a lower light-screening effect, lower organic carbon content, and higher proportions of carbonate minerals and quartz components conducive to the photodegradation of o,p′-DDT on the SPM surface. The compositional characteristics of endogenous dissolved organic matter (DOM) also influenced the generation of reactive oxygen species (ROS) by SPM. Mass spectrometry analysis revealed that although the SPM from different basins did not alter the types of o,p′-DDT products, it influenced their yields. The estrogenic effects of the products were preliminarily assessed through molecular docking with the human estrogen receptor, and the phototransformation process on the surface of Haihe River SPM might have a more substantial estrogenic effect. These findings deepened the understanding of the regional differences in pollutant fate and might provide reference information for the management and regulation of pollutants in different regions.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）