MOLECULES
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2023
·Vol. 28
·期 8
·3542
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IF 4.6
Qing Liu,Chao Yan,Yan Wang
Applications of submicron-scale particles are of rising interest in separation science due to their favorable surface-to-volume ratio and their fabrication of highly ordered structures. The uniformly dense packing beds in columns assembled from nanoparticles combined with an electroosmotic flow-driven system has great potential in a highly efficient separation system. Here, we packed capillary columns using a gravity method with synthesized nanoscale C18-SiO2particles having diameters of 300–900 nm. The separation of small molecules and proteins was evaluated in the packed columns on a pressurized capillary electrochromatography platform. The run-to-run reproducibility regarding retention time and peak area for the PAHs using a column packed with 300 nm C18-SiO2particles were less than 1.61% and 3.17%, respectively. Our study exhibited a systematic separation analysis of small molecules and proteins based on the columns packed with submicron particles combined with the pressurized capillary electrochromatography (pCEC) platform. This study may provide a promising analytical approach with extraordinary column efficiency, resolution, and speed for the separation of complex samples.Keywords:submicron scale particles;pressurized capillary electrochromatography;separation
研究领域:
分析化学
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分离科学
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DOI
PubMed
高影响因子
RSC Advances
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2023
·Vol. 13
·期 28
·19455-19463
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IF 3.9
Xiao-Quan Liu,Chao-Feng Qin,Nai-Dong Chen,Jing-Wen Hao,Shi-Tang Ma,Min Zhang,Yu Song,Yun-Jiang Min,Ya-Qin Bu,Sheng Li
A rapid pressurized capillary electrochromatography (pCEC) method has been established for the simultaneous analysis of 11 phenols in the four main original plants of the famous traditional Chinese medicine (TCM) Shihu. The effects of wavelength, mobile phase, flow rate, pH value, concentration of buffer, and applied voltage were systematically studied. The investigated 11 phenols could be isolated in 35 min on a reversed-phase EP-100-20/45-3-C18 capillary column using the established method. To apply the established pCEC method, all phenols except tristin (11) were detected in the four Dendrobium plants. A total of 10 components were detected in D. huoshanense, 6 components in D. nobile, 3 components in D. chrysotoxum, and 4 components in D. fimbriatum. The consistent evaluation revealed that the similarities among the four original plants of Shihu were 38.2–86.0% based on the 11 polyphenols and 92.5–97.7% based on the pCEC fingerprints. These further suggested that the components of the four original plants of TCM Shihu might be significantly different. Further investigation should be conducted to confirm and evaluate if the four species could be used as the same medicine with the same amount according to Chinese Pharmacopoeia (ChP).
研究领域:
分析化学
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传统医学
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药理学
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DOI
PubMed
高影响因子
Journal of Energy Chemistry
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2025
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IF 14.9
Chenhao Wang,Xiaoyun Miao,Ting Luo,Xingzhi Zhu,Xiaofeng Ye,Zhaoyin Wen
CO 2 hydrogenation using protonic ceramic electrolysis cells (PCECs) to produce fuel gases such as CH 4 and CO has been considered as a promising technology for effective CO 2 utilization. However, the long-term stability of conventional PCECs based on Y and Yb doped BaZrO 3 -BaCeO 3 (BCZYYb) proton conductors is severely limited by their susceptibility to carbonate formation under high concentration CO 2 . In this work, a new type PCEC based on CO 2 -tolerant La 5.6 WO 11.4−δ (LWO) material is firstly constructed. A three-layer porous-dense-porous LWO ceramic scaffold is fabricated via a pressing, dip-coating and co-sintering process, followed by Ni and La 0.6 Sr 0.4 CoO 3−δ (LSC) catalyst impregnation to ensure the chemical compatibility among materials and form an efficient PCEC-based CO 2 hydrogenation reactor. Benefits from the synergistic catalysis of nano nickel and LWO, the reactor gets a CH 4 selectivity of over 50 % at 600 °C under 20 % CO 2 concentration, and it operates stably for over 320 h at 600–650 °C under high CO 2 concentrations of 50–80 %, showing no degradation in CO 2 conversion rate or CH 4 selectivity. Post-mortem analysis demonstrates that the CO 2 absorption characteristics of LWO and the morphological uniformity of nano nickel lead to stable CO 2 methanation. This study provides a viable strategy for designing highly stable PCEC-based CO 2 hydrogenation reactors.
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高影响因子
ANALYTICAL SCIENCES
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2023
·Vol. 39
·期 6
·925-933
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IF 1.6
Tang Tingfan,Li Xiaohui,Zhu Jiaqing,Huang Wenyi,Cheng Hao,Feng Jun
In this paper, a polymer monolithic column based on poly (Butyl methacrylate-co-ethylene glycol dimethacrylate) (poly (BMA-co-EDGMA)) doped with MIL-53(Al) metal–organic framework (MOF) was prepared using an in situ polymerization method. The characteristics of MIL-53(Al)-polymer monolithic column were studied through scanning electron microscopy (SEM), Fourier transform infrared spectrometry (FT-IR), energy-dispersive spectroscopy (EDS), X-ray powder diffractometry (XRD), and nitrogen adsorption experiment. Due to its large surface area, the prepared MIL-53(Al)-polymer monolithic column has good permeability and high extraction efficiency. Using MIL-53(Al)-polymer monolithic column for solid-phase microextraction (SPME), coupled to pressurized capillary electrochromatography (pCEC), a method for the determination of trace chlorogenic acid and ferulic acid in sugarcane was established. Under optimized conditions, chlorogenic acid and ferulic acid have a good linear relationship ( r ≥ 0.9965) within the concentration range of 50.0–500 µg/mL, the detection limit is 0.017 µg/mL, and the relative standard deviation (RSD) is less than 3.2%. The spike recoveries of chlorogenic acid and ferulic acid were 96.5% and 96.7%, respectively. The results indicate that the method is sensitive, practical, and convenient. It has been successfully applied to the separation and detection of trace organic phenolic compounds in sugarcane samples. Graphical abstract
研究领域:
分析化学
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食品科学
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环境科学
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DOI
PubMed
高影响因子
BIOMATERIALS
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2020
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IF 10.317
Zhiguang Qiao,Meifei Lian,Yu Han,Binbin Sun,Xing Zhang,Wenbo Jiang,Huiwu Li,Yongqiang Hao,Kerong Dai
Despite significant advances in osteochondral tissue engineering, it remains challenging to successfully reconstruct native-like complex tissues organized in three-dimension with spatially varying compositional, structural and functional properties. In this contribution, inspired by the gradients in extracellular matrix (ECM) composition and collagen fiber architecture in native osteochondral tissue, we designed and fabricated a tri-layered (superficial cartilage (S), deep cartilage (D) and subchondral bone (B) layer) stratified scaffold in which a mesenchymal stem cell (MSC)-laden gelatin methacrylamide (GelMA) hydrogel with zone-specific growth factor delivery was combined with melt electrowritten triblock polymer of poly(ε-caprolactone) and poly(ethylene glycol) (PCEC) networks with depth-dependent fiber organization. Introducing PCEC fibers into the weak GelMA hydrogel contributed to a significant increase in mechanical strength . In vitro biological experiments indicated that the stratified fiber-reinforced and growth factor-loaded hydrogel construct induced the MSCs to differentiate down both the chondrogenic and osteogenic lineages and that the engineered complex exhibited cellular phenotype and matrix accumulation profiles resembling those of the native tissue. Simultaneous cartilage and subchondral bone regeneration were achieved in vivo by using the tri-layered integrated scaffold. More importantly, the inclusion of the S layer could impart the regenerated cartilage with a more lubricating and wear-resistant surface. These findings suggest that the bioinspired construct mimicking the spatial variations of native osteochondral tissue might serve as a promising candidate to enhance osteochondral regeneration.
研究领域:
组织工程
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生物医学工程
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材料科学
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再生医学
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DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2021
·
IF 6.165
Jing Lv,Meiqiang Xie,Shufen Zhao,Wensheng Qiu,Shasha Wang,Manming Cao
Hybrid-drugs chemotherapy is now one of the widely adopted treatments for cancer prevention with favorable diagnoses. This work has proposed compostable paclitaxel (PTX) and biotin (BT) PCEC nanomaterials (NMs) for the codelivery and to investigate the drug delivery mechanism's efficacy ( [email protected] ) against gastric cancer cells in vitro and in vivo. The fabricated [email protected] were 27.97 ± 1.87 nm in size and had a less polydispersity index (0.156 ± 0.030). PTX and BT were released slowly from the drug delivery without any burst impact. In addition, dose-responsive cytotoxic effects were observed for [email protected] on SGC-791 cells with a higher mortality ratio than free drugs. According to a cellular uptake investigation, drug-loaded PCECs of polymeric liquid nanoparticles were considered better by cancer cells in vitro. To assess the in vivo antitumor activity, [email protected] were administered intravenously injected to xenografted with SGC-791 cells. Substantial inhibition of tumor development was observed with extended survival time and decreased side effects with [email protected] compared to free drugs (PTX + BT). Furthermore, compared to other therapy classes, [email protected] care resulted in lower H&E and Ki67 expressions and improved TUNEL positive in cancer cells, indicating the chemotherapeutic efficiency of drug delivery system. Overall, the current investigation confirmed that DDS [email protected] could be used to successfully combat gastric cancers in the immediate future.
研究领域:
药学
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纳米技术
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肿瘤学
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药物递送系统
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DOI
高影响因子
危险品化学品经营许可证（带存储）