Ming Zhang,Jing Wang,Wentao Wang,Jun Zhang,Ninglin Zhou
Carbon dots could be extended in biological applications for drug delivery and photothermal therapeutics due to their ability to improving therapeutic effect and reducing side effects. Here, we use doxorubicin (DOX) as the model drug, the amino and carboxyl coated magnetofluorescent carbon quantum dots (GdN@CQDs) as a carrier, Genipin (GP) as cross-linking agent to design and prepare a multifunctional drug delivery system triggered by near-infrared (NIR) light and pH dual-stimuli. The cytotoxicity of the nanoparticles has been examined by MTT assay. The fluorescent GdN@CQDs/GP serves as both a controllable drug release gatekeeper and a fluorescent probe for the visualization of the drug delivery process. In vitro and in vivo experiments showed that this platform can deliver anti-cancer drugs to cells, releasing them intracellular upon NIR irradiation, and effectively eliminate tumors through chemo-photothermal synergistic therapeutic effect.
Yang Shu,Rusheng Song,Anqi Zheng,Jingli Huang,Mingli Chen,Jianhua Wang
A thermo/pH dual-stimuli-responsive drug delivery system (DDS) based on polymer coated mesoporous silica nanostructures (MSNs) is developed for facilitating chemotherapy and photothermal therapy. Thermo/pH-responsive polymer, poly((N-isopropylacrylamide, NIPAM)- co -methacrylic acid, MA), is grafted onto MSNs by in situ polymerization, followed by loading a chemotherapeutic drug (doxorubicin hydrochloride, DOX) and a near-infrared-absorbing phototherapeutic agent (indocyanine green, ICG) to construct the intelligent drug delivery system, shortly as [email protected] (NIPAM- co -MA). At NIR irradiation, the photothermal conversion capability of ICG raises the temperature of the DDS and opens the gatekeeper by shrinkage of the copolymer p(NIPAM- co -MA), which triggers controlled release of DOX at an elevated temperature. On the other hand, drug release is also realized at pH 5.3, a characteristic pH value in cancer cell microenvironment, at which it not only causes the shrinkage of the pH-sensitive polymeric moiety of methacrylic acid in [email protected] (NIPAM- co -MA) but also deteriorates electrostatic interaction of DOX molecules in the mesoporous channel by protonation of silanols . In addition, ICG further ensures photothermal therapy (PTT) and photodynamic therapy (PDT). The cytotoxicity assay of HeLa cells shows obvious synergistic effect by demonstrating that the combined use of DOX and ICG is more effective in killing HeLa cells than free DOX and ICG. The endocytosis of the drug is monitored by cell imaging.
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