JOURNAL OF POLYMER SCIENCE PART A-POLYMER CHEMISTRY
·
2018
·Vol. 56
·期 13
·1412-1423
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IF 2.588
Nigus Mesele Tsegay,Xiang-Yun Du,Si-Si Liu,Cai-Feng Wang,Su Chen
Frontal polymerization (FP) is applied for the synthesis of β-cyclodextrin/poly(vinylimidazole- co -N-vinylcaprolactam- co -acrylic acid) (β-CD/P(VI- co -NVCL- co -AA)) copolymers. The dependence of frontal velocity and temperature on the initiator and cross-linker are discussed. The synthesized copolymers have been characterized by Fourier transform infrared (FTIR), thermogravimetric analysis (TGA), and scanning electron microscopy (SEM). The thermo-pH dual-stimuli responsive behavior of the hydrogel is determined by swelling measurement at different temperatures and pH values. Besides, the hydrogels show intrinsic self-healing behavior and their healing efficiency is determined by the mechanical tests. Interestingly, we integrate FP with microfluidic technology, which may realize the execution of FP under continuous condition. Such simple microfluidics-FP integrated approach has both methodological and practical value for the synthesis of functional materials. This paper mainly presents the synthesis and characterization of β-cyclodextrin/poly(vinylimidazole- co -N-vinylcaprolactam- co -acrylic acid) (β-CD/P(VI- co -NVCL- co -AA)) copolymers by using thermal frontal polymerization (TFP). Hydrogels were found to be self-healing with good mechanical performance and show dual thermo-pH responsive behavior. Low-cost, energy-saving and efficient method of thermal frontal polymerization process was integrated with microfluidics technology to prepare supraball hydrogel. © 2018 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2018 , 56 , 1412–1423
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
COLLOID AND POLYMER SCIENCE
·
2023
·Vol. 301
·期 5
·455-464
·
IF 2.4
Jong Kwanghyok,Ju Benzhi,Kim Ganghyok,Kim Kwangjin
A novel type of temperature-pH dual-responsive amphiphilic hydrogel, PD(hydroxyethyl starch(HES)-propynyl glycidylether(PGE)-2-dimethylamino ethanethiol (DMAET)-triethoxyvinylsilane (TEVS)) was synthesized and characterized. HES, a hydrophilic polysaccharide, underwent etherification of hydroxyl groups in its glucose units with PGE and then click reaction between carbon–carbon triple bonds in PGE and thiol groups in DMAET thereby possessing a hydrophilic-hydrophobic structure to be used for the preparation of PD, a temperature-pH dual-responsive material, with pH-responsive tertiary amine groups. Next, it further became a dual-responsive hydrogel through the formation of its internal network by TEVS. The hydrogel exhibited its maximum hydroswelling ratio of 2000 and a good transference between its shrinking and swelling. Moreover, during the test experiments to use it as a drug carrier for oral delivery, a doxorubicin (DOX) release was ≤ 15% in acidic conditions in the stomach (pH < 2.0) and ≤ 94% in basic conditions in the small intestine, ensuring that it exhibits relatively good protective and release properties of drugs.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Yuanyuan Wang,Weimin Guan,Yulin Yang,Huiling Lan,Yu Wang,Yun Wang,Juan Han,Lei Wang
Immobilization of enzymes in porous organic framework (POF) materials is popular strategy to stabilize enzymes. For such solid enzyme catalysis system, improving the catalytic efficiency is challenging due to the diffusion resistance from solid-liquid interface and inner pores. Here, UCST-pH dual responsive polymeric carrier (PEG- b -PAAm- b -P(GMA- co -AAc)) was synthesized to immobilize cytochrome c (Cyt c), which impart the reversibly insoluble-soluble property to the immobilized Cyt c. The PEG- b -PAAm- b -P(GMA- co -AAc) could serve as an insoluble-soluble matrix to fast and efficiently immobilize Cyt c via covalent attachment, achieving a remarkable 92 % loading efficiency within just 120 min. The obtained insoluble PEG- b -PAAm- b -P(GMA- co -AAc)-Cyt c micelles exhibited an improvement in thermal, pH stability and reusability. The completely soluble PEG- b -PAAm- b -P(GMA- co -AAc)-Cyt c conjugates accelerated substrate diffusion and then enhanced the catalytic efficiency. These excellent advantages led to low detection limit (1.99 μM), lower than the presently reported biosensors based on enzyme mimics in the colorimetric detection of phenol. This UCST-pH dual responsive window presents a new platform to efficiently control the immobilization and release of enzymes, which will achieve excellent stability and catalytic efficiency.
研究领域:
生物化学
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材料科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2012
·Vol. 2
·期 31
·11953-11962
·
IF 0
Dali Wang,Xiuying Huan,Lijuan Zhu,Jinyao Liu,Feng Qiu,Deyue Yan,Xinyuan Zhu
A new type of salt/pH dual-responsive micelles based on supramolecular amphiphilic brush copolymers, poly(2-hydroxyethyl metharylate)-g-(poly(ε-caprolactone)-adenine:uracil-poly(ethylene glycol)) (PHEMA-g-(PCL-A:U-PEG)) was developed for the anticancer drug delivery owing to the fact that the tumor tissues show low pH and high salt concentration. The supramolecular structure of brush copolymer was confirmed by variable-temperature 1H NMR and Fourier transform infrared spectroscopy (FTIR). Doxorubicin (DOX) as a model anticancer drug was efficiently loaded into the supramolecular micelles (up to 70%) due to the compact structure of brush polymer. The cumulative release profile of the DOX-loaded micelles showed a low level of drug release (about 20 wt% in 25 h) at pH 7.4 with a low salt concentration, and was significantly accelerated at a lower pH (5.0) and a high salt concentration (over 70 wt% in 6 h), exhibiting an salt/pH dual-responsive controlled drug release capability. Methyl tetrazolium (MTT) assay showed that DOX-loaded micelles had high anticancer efficacy against Hela cancer cells and blank micelles had a very low cytotoxicity. These supramolecular brush copolymer micelles possess many favorable traits, such as low cytotoxicity and excellent biodegradability, adequate drug loading capacity, and rapid drug release in response to the intracellular level of pH and salt concentration, which endow them as a promise candidate for delivering anticancer drugs.
研究领域:
高分子化学
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药剂学
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肿瘤学
✓ 已验证
DOI
MATERIALS & DESIGN
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2016
·
IF 3.997
Xu Jia,Kun Tian,Xubo Zhao,Tingting Zhou,Mingliang Pei,Peng Liu
Novel reduction/pH dual-stimuli responsive fluorescent amphiphilic copolymer PEG-Cys-P(DEAEMA-co-Rh6GEAm) with detachable poly(ethylene glycol) (PEG) corona through bio-reducible disulfide linkage and pendent cationic groups was synthesized via reversible addition-fragmentation chain transfer (RAFT) polymerization for tumor theranostic application. The doxorubicin (DOX)-loaded micelles could be easily fabricated via facile micellization in the presence of DOX in water, with a high drug-loading capacity (DLC) of 39.2%. They exhibited fast release ratio with the synergetic effect of reductive agent and pH in the simulated tumor micro-environment, while the leakage can be efficiently avoided in the physiological media. Furthermore, the fluorescence intensity of the copolymer possessed a reversibly transformation in different pH media, indicating the potential application for intracellular fluorescence imaging of tumors.
研究领域:
生物材料学
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肿瘤治疗学
·
药剂学
✓ 已验证
产品关联
DOI
高影响因子
BIOMATERIALS
·
2012
·
IF 7.404
Yuan-Jia Pan,Yuan-Yuan Chen,Dong-Rui Wang,Chuan Wei,Jia Guo,Da-Ru Lu,Chih-Chang Chu,Chang-Chun Wang
A new type of redox/pH dual stimuli-responsive poly(methacrylic acid) (PMAA)-based nanohydrogels was prepared from methacrylic acid and N,N -bis(acryloyl)cystamine crosslinker via distillation-precipitation polymerization. The nanohydrogels could be easily degraded into individual linear short chains (M n ≈ 1200, M w /M n < 1.1) in the presence of 10 mM dithiothreitol (DTT) or glutathione (GSH). Doxorubicin (DOX) as a model anti-cancer drug was high efficiently loaded into the nanohydrogels (up to 42.3 wt%) due to the strong electrostatic interactions between the amine group in doxorubicin (DOX) and the carboxyl groups in the nanohydrogels at a physiological pH. The cumulative release profile of the DOX-loaded nanohydrogels showed a low level of drug release (less than 15 wt% in 24 h) at pH 7.4, and was significantly accelerated at a lower pH (5.0) and reducing environment (over 91 wt% in 5 h), exhibiting an obvious pH/redox dual-responsive controlled drug release capability. The drug release behavior of the DOX-loaded nanohydrogels in the presence of GSH was very different from the DTT as the loaded DOX could be quickly released in the presence of GSH, but not in DTT. The possible reason is the synergic effect of reduction and charge exchange of GSH at a low pH. The dose-dependent cytotoxicity of the DOX-loaded nanohydrogels was studied by the CCK-8 assay; the DOX-loaded nanohydrogels could be taken up quickly by human glioma (U251MG cells) via endocytosis , and then biodegraded to release the loaded drugs, which exhibited a comparably anti-tumor efficacy. These nanohydrogels possess many favorable traits, such as excellent biocompatibility and biodegradability , adequate drug loading capacity, minimal drug release under an extracellular condition (non-reductive), and rapid drug release in response to the intracellular level of pH and reducing potential, which endow them as a promise candidate for delivering anti-cancer drugs.
研究领域:
生物材料学
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药剂学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
OPTICAL MATERIALS
·
2018
·
IF 2.32
Jun Wang,Si Chen,Xiaoyan Cheng,Qi Peng,Wuke Li
Thermo/pH dual-responsive luminescent composites consisting of lanthanide-polyoxometalates and Poly (N-isopropylacrylamide)/Chitosan were synthesized. The dual-responsive nanoparticles are characterized by the FT-IR, UV–vis, TEM , SEM , TGA, DLS and luminescence. The prepared composites exhibit the thermo/pH-responsive properties and the controllable and switchable luminescence in solution in response to the simple changes in temperature and pH values. For instance, the composite hydrogels exhibit intense red luminescence under UV irradiation below 34 °C, and the luminescence become much weaker in solution above 34 °C. Moreover, the hydrogel could not show red luminescence in the buffer solution with low pH value. However, it shows bright red luminescence in neutral and alkaline buffer solution. The possible reasons for controllable luminescence are discussed in the research. The results suggest that the multifunctional nanoparticles could be potentially used in biomedical fields such as luminescent switches, biological labeling and imaging.
研究领域:
生物医学
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材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Jingfan Xie,Guanqun Zhong,Changqun Cai,Chunyan Chen,Xiaoming Chen
As biomarkers of many diseases, glycoproteins are of great significance to clinical diagnostics. However, the determination of low abundant glycoproteins in complex biological samples without any pretreatment process is still a problem. In this study, a rapid and convenient separation method for highly efficient enrichment of glycoproteins is reported, based on pH double-responsive imprinted magnetic microspheres . Thin imprinted polymer shells were fabricated onto the surface of magnetic microspheres by free radical polymerization , using 2-(Dimethylamino) ethyl methacrylate as pH-sensitive monomer , 4-vinylphenylbronic acid as boronate affinity monomer, and ovalbumin (OVA) as template molecule. Combining the advantages of pH-sensitive monomer and boronate affinity monomer, rapidly capture-release of OVA could be modulated by changing solution pH. Moreover, high absorption ability (81.2 mg/g) was achieved within about 10 min. This study provided responsible way to imprint glycoproteins and showed great potential for glycoprotein detection in clinical diagnostic.
研究领域:
分析化学
·
生物技术
·
材料科学
·
临床诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Sihan Shen,Ran Xie,Yiqun Wang,Lingyu Jia,Zhangran Zhu,Hai Ma,Lifang Wang,Feng Sui,Yu Zhao,Qinghe Zhao,Yanjun Chen
Ferroptosis represents a novel procedure of programmed cell death driven by iron-dependent lipid peroxidation, and may offer a promising strategy for cancer therapy. In this study, we developed poly (2-ethyl-2-oxazoline)-poly (lactic acid)-SS-poly (β-amino ester) (PEOz-PLA-SS-PBAE) loaded with β-elemene. The disulfide bond mediated depletion of glutathione (GSH) and inactivation of glutathione peroxidase 4 (GPX4) will lead to inactivation of the antioxidant system of the tumor cell, and β-elemene has the capacity to generate reactive oxygen species (ROS), and the above changes would induce a disruption of the redox balance within the tumor cell, resulting in ferroptosis of the tumor cell. In conclusion, in this study, water insoluble β-elemene was solubilized by the triblock copolymer to construct a pH/GSH dual-responsive nano-delivery system, and combined with iron death and chemotherapy to exert combinatory inhibition effect on breast cancer cells, which provides a new idea for the potential clinical application of β-elemene
研究领域:
肿瘤学
·
药学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Bioactive Materials
·
2023
·
IF 18.9
Qun Li,Jinbiao Liu,Huijie Liu,Yue Sun,Yingying Xu,Kexin Wang,Wenjing Huang,Lan Liao,Xiaolei Wang
Caries is considered to be the most prevalent non-communicable disease in humans, mainly deriving from acidogenic bacterial biofilm and resulting in the demineralization and decomposition of hard dental tissue. Herein, a composite responsive foam brace loaded with magnesium organic framework (MPC) is designed for caries prevention and tooth remineralization. MPC can intelligently release organic antibacterial molecules (gallic acid) and mineralized ions (Mg 2+ , Ca 2+ and PO 4 3− ) under acidic conditions (pH < 5.5) of biofilm infection, regulating pH and killing bacteria. Additionally, due to the excellent photothermal conversion efficiency, MPC can further enhance the destruction of bacterial biofilm by inhibiting virulence genes and destroying bacterial adhesion under near-infrared light irradiation (808 nm). More importantly, MPC can not only reverse the cariogenic environment at both pH and microbial levels, but also promote self-healing of demineralized teeth in terms of both the micro-structure and mechanical properties.
研究领域:
牙科材料
·
生物材料
·
光疗
·
口腔健康
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Guanghui Hou,Junmin Qian,Weijun Xu,Tiantian Sun,Jinlei Wang,Yaping Wang,Aili Suo
The combination of chemotherapy and photothermal therapy is acknowledged as one of the most promising approaches in cancer treatment. Targeted delivery and controlled drug release are two important factors for combined chemo-photothermal therapy. In this study, a multifunctional nanoplatform based on gold nanorod (GNR) decorated with folate-conjugated poly(ethylene glycol)- b -poly(L-γ-glutamylhydrazine) (FEGGH) containing disulfide linker and dihydroxyphenyl groups was developed for targeted combined chemo-photothermal therapy of breast cancer. FEGGH was synthesized by ring-opening polymerization of γ-benzyl- l -glutamate -N- carboxyanhydride using folate/cystamine-heterobifunctionalized poly(ethylene glycol) as an initiator, following by hydrazinolysis and carbodiimide reactions. FEGGH was decorated onto GNR through Au-catechol bonds. Chemotherapeutic drug doxorubicin (DOX) was loaded onto the nanoplatform through pH-sensitive hydrazone linkage, obtaining final product FEGGH DOX -GNR. The DOX-loaded nanoplatform displayed excellent photostability and reduction/pH dual-responsive drug release behavior. Cytological studies demonstrated the effective internalization of FEGGH DOX -GNR into MCF-7 cells via folate-mediated endocytosis and additive therapeutic effect of combined photothermal-chemotherapy. These results indicate that our nanoplatform may be a promising strategy for targeted combined chemo-photothermal therapy of breast cancer.
研究领域:
纳米医学
·
癌症治疗
·
材料科学
·
聚合物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 15
·2665-2673
·
IF 5.687
Ying Wang,Qiaojie Luo,Weipu Zhu,Xiaodong Li,Zhiquan Shen
In this work, an amphiphilic alternating multiblock copolymer poly[oligo(ethylene glycol)fumarate-co-dithiodiethanol fumarate] (POEGSSFM) with multiple enes and disulfides in hydrophobic blocks was synthesized facilely by the “one-pot” method, which can self-assemble into nano-scaled micelles and encapsulate mercapto-modified doxorubicin (DOX-SH) with 1,6-hexanedithiol into the core for in situ drug conjugation and core-crosslinking (CCL) via a thiol–ene “click” reaction, resulting in a reduction and pH dual-responsive nano-prodrug micelle (CCL-POEGSSFM-DOX). The obtained nano-prodrug micelles presented stable nano-scaled spherical particles under physiological conditions, while quickly dissociating in response to 10 mM DL-dithiothreitol (DTT). As doxorubicin (DOX) was conjugated via a pH-sensitive hydrazone linkage, the in vitro release results showed a minimized release of DOX at pH 7.4, while a rapid release at pH 5.8. Particularly, the release of DOX in an acidic environment could also be significantly accelerated by treating with DTT, owing to the cleavage of the disulfide bonds, which leads to the disassociation of the micelles. Confocal laser scanning microscopy further demonstrated that the nano-prodrug can be easily taken into cells, presenting a rapid DOX release in the cytoplasm. Cytotoxicity assay indicated that the nano-prodrug micelles exhibited excellent cytotoxic effects on HeLa cells, while blank CCL-POEGSSFM micelles were biocompatible. This facilely prepared pH and reduction dual-responsive CCL nano-prodrug would be a promising candidate for cancer chemotherapy.
研究领域:
药学
·
纳米技术
·
癌症研究
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）