SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Zhongjiao Feng,Jiasheng Wu,Meiyu Jiang,Jie Sha,Weimin Liu,Haohui Ren,Wenjun Zhang,Chun-Sing Lee,Pengfei Wang
The pH homeostasis of Golgi plays a vital role in maintaining normal cell functions. An effective near-infrared fluorescent probe that can sense pH changes in Golgi apparatus is of great significance. Here, a Golgi-targeting fluorescent probe ( Golgi-hNR) based on a rhodamine derivative is presented to show a highly sensitive response to pH variations. Golgi-hNR displays a conspicuous pH-dependent behavior at 664 nm from pH 7.4–3.0 with a remarkable fluorescence off-on response. The pKa value of Golgi-hNR is measured to be 3.62. Confocal fluorescence imaging reveals that Golgi-hNR diffuses into Golgi with high selectivity . Using Golgi-hNR , we have successfully visualized real-time tracking of Golgi pH variations for medical therapy in living cells.
研究领域:
细胞生物学
·
医学
✓ 已验证
DOI
高影响因子
NANO
·
2014
·
IF 1.26
JUN LI,BIN KANG,SHUQUAN CHANG,YAODONG DAI
We directly visualized the uptake and intracellular vesicle transport of shortened single walled carbon nanotubes in living cells through a dual-labeling system. With a stable labeling of fluorescein isothiocyanate and a pH-sensitive stacking of doxorubicin on carbon nanotubes, the location of internalized nanotubes inside the cell and the microenvironment especially the pH change of the nanotube-embedded vesicles could be monitored at the same time. Results showed that after internalization through endocytic pathway, carbon nanotubes tended to transport from the early endosomes to later acidic lysosomes and these vesicles moved along the microtubule track toward a perinuclear region where is a microtubule-organizing center. These results might provide a novel understanding for the intracellular interaction of carbon nanotubes and living cells.
研究领域:
细胞生物学
·
纳米医学
·
生物成像
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Feng Li,Wei Wang,Chengcheng Li,Runliang Zhu,Fei Ge,Yang Zheng,Yixin Tang
Biomineralization is an interesting naturally occurring process of forming minerals by microorganisms, which offers an efficient way to sequester heavy metal ions within relatively stable solid phases. In this study, Bacillus cereus Cd01 was selected to investigate effects of self-mediated pH on biosorption and biomineralization of Cd 2+ in whole 72h cultivation period. Results revealed that strain Cd01-mediated pH decrease of the cultivation medium from 7.0 to 6.1 inhibited biosorption of Cd 2+ on Cd01 cells at the initial cultivation period, while an increased pH from 6.1 to 7.4 facilitated biosorption of Cd 2+ on Cd01 cells at the middle and late cultivation period. The reasons were mainly that self-mediated pH altered cell surface hydrophobicity and cell membrane fluidity of strain Cd01. Moreover, biosorption and bioaccumulation of Cd 2+ on Cd01 cells in the period of increased pH promoted biomineralization of Cd 2+ observed by the transmission and scanning electron microscopes. The analyses of energy dispersive spectroscopy, X-ray photoelectron spectroscopy and select area electron diffraction demonstrated that Cd 2+ loaded on Cd01 cells was biomineralized into polycrystalline and/ or amorphous cadmium sulfide and cadmium phosphate. These results suggest that strain Cd01 may play a potential role in biomineralization remediation of heavy metal contaminated soils.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Jing Tian,Jingqian Chen,Jiaqi Guo,Wenyuan Zhu,Mohammad Rizwan Khan,Qingquan Fu,Yongcan Jin,Huining Xiao,Junlong Song,Orlando J. Rojas
We introduce Kraft lignin colloids produced by destabilization of a kraft lignin solution by pH shift from 2 to 11. The system includes lignin particles and hydrocolloid aggregates in equilibrium (KLP), which are used to control the emulsifying energy and to stabilize oil-in-water emulsions under high energy at pH 11 and 7, yielding excellent stability (at least three months with no creaming). We investigate emulsify technology (ultrasonication and homogenization), energy input (high energy or low energy), particle desorption, drop size, and rheology on the stability of KLP-based Pickering emulsions according to the system’s pH. The ultrasonication leads to fine, stable and homogenous lignin emulsions without observing macroscopic creaming phenomenon, whereas homogenization only limits to alkaline condition. Regarding homogenization process, increasing the energy input helps to reduce the emulsion droplet size and consequently improves emulsion stability. In addition, the emulsion produced at acidic conditions always presented excellent stability, independent of the emulsify parameters. Considering the synergistic effects of emulsification protocol, surface activity, and particle interactions, the results indicate the possibility of extending the applications of kraft lignin as a Pickering stabilizer in neutral and alkaline conditions. It also creates a promising method to control the stability and emulsion microstructure in the industrial KLP based emulsion preparation process.
研究领域:
食品科学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Sensor and Energy Materials
·
2022
·
IF 0
Jia Gao,Rui-Yang Zhao,Yi-Guang Wang,Ruo-Chen Xie,Wei Wang
We present a fluorescent microscopic method using an ultra-pH-sensitive polymeric probe to rapidly map within subsecond the pH distribution resulting from oxygen reduction reaction electrocatalysed by an array of platinum nanoparticles. Upon voltammetry of the surface-supported Pt catalysts, fluorescent quenching waves are observed to depend on the electrode potential. The spatiotemporal fluorescent evolution is then confirmed under a constant potential control to be due to the local pH change as a function of diffusing time by an estimation of the proton diffusion coefficient ( L α t 1 / 2 ) . On these bases, the fluorescent measurements at short reaction times can provide quantitative information regarding the one and two dimensional pH distributions, which are shown to exhibit the expected shape of a typical diffusion-driven concentration gradient. Such imaging of proton/pH profiles may find important applications such as efficient screening of different micro/nanoscale electrocatalysts.
研究领域:
分析化学
·
电化学
·
材料科学
·
生物传感器
✓ 已验证
DOI
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yongtang Chen,Xueyan Li,Junshuo Lian,Kang Fu,Zhongxi Zhao,Yi He,Peng Tan
Aqueous rechargeable batteries are regarded as one of the most reliable solutions for electrochemical energy storage, and ion (e.g., H+ or OH–) transport is essential for their electrochemical performance. However, modeling and numerical simulations often fall short of depicting the actual ion transport characteristics due to deviations in model assumptions from reality. Experimental methods, including laser interferometry, Raman, and nuclear magnetic resonance imaging, are limited by the complexity of the system and the restricted detection of ions, making it difficult to detect specific ions such as H+ and OH–. Herein, in situ visualization of ion transport is achieved by innovatively introducing laser scanning confocal microscopy. Taking neutral Zn–air batteries as an example and using a pH-sensitive probe, real-time dynamic pH changes associated with ion transport processes are observed during battery operation. The results show that after immersion in the zinc sulfate electrolyte, the pH near the Zn electrode changes significantly and pulsation occurs, which demonstrates the intense self-corrosion hydrogen evolution reaction and the periodic change in the reaction intensity. In contrast, the change in the pH of the galvanized electrode plate is weak, proving its significant corrosion inhibition effect. For the air electrode, the heterogeneity of ion transport during the discharging and charging process is presented. With an increase of the current density, the ion transport characteristics gradually evolve from diffusion dominance to convection-diffusion codominance, revealing the importance of convection in the ion transport process inside batteries. This method opens up a new approach of studying ion transport inside batteries, guiding the design for performance enhancement.
研究领域:
电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Lei Xie,Yiping Zhang,Ling Chen,Tianxing Wang,Shuo Zhang,Xiaoxi Li
Characterizing the structural changes of cell-targeting delivery carriers in gastrointestinal tract (GIT) is crucial for understanding their effectiveness in cell targeting and transport. Herein, RGD peptide-grafted carboxymethyl starch (CMS) and cationic quaternary ammonium starch (QAS) were utilized to fabricate quintet-layered nanocapsules loaded with ovalbumin (OVA). The aim was to improve delivery and transportation efficiency, specifically targeting M cells. The research analyzed the impact of pH and enzyme variations in GIT on the structure of nanocapsules, interactions between carriers and the release behavior of OVA. Results showed that the size of nanocapsules increased from 229.2 to 479.8 nm and the zeta potential decreased from −1.08 to −33.33 mV during oral delivery. This was evident in TEM images, showing a more relaxed core-shell structure. Isothermal titration calorimetry and molecular dynamic simulation indicated that pH changes primarily affected the electrostatic interaction between carriers. Increasing pH led to reduced affinity constants, and around 84.42 % of OVA was successfully delivered to M cells. Moreover, the transport efficiency of nanocapsules to M cells was five times greater than that of Caco-2 cells. This suggests the feasibility of developing a nanocapsules delivery system capable of adapting to pH changes in GIT by regulating electrostatic interactions between carriers.
研究领域:
食品科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2018
·
IF 4.513
Guang Zhao,Xuepeng Wu,Rizhong Luan,Shaikh Azizullah,Haiming Fan,Caili Dai
A novel CO 2 responsive anionic smart wormlike micelles (SWLMs) was constructed by simply mixing sodium oleate (NaOA) and the small organic counterion 2, 6, 10-trimethyl-2, 6, 10-triazaundecane (TMTAD) in a 3:1 M ratio without complex organic synthesis. After bubbling CO 2 , TMTAD and NaOA molecules in aqueous solution, a pseudo Gemini surfactant is formed in situ due to electrostatic interactions. This system shows two obvious performance transitions when CO 2 is continuously introduced, from a water-like solution to a viscoelastic fluid and then a milky solution. Such transitions reflect the continuous transformation of the self-assembled structure from spherical micelles to wormlike micelles and then vesicle or spherical micelles. Additionally, this system can exhibit two similar sequential transformations when the pH changes in the reverse process.
研究领域:
材料科学
·
化学工程
·
纳米技术
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2015
·
IF 6.723
Lujing Chai,Jin Zhou,Hui Feng,Cong Tang,Yuanyuan Huang,Zhaosheng Qian
Sensitive assay of tyrosinase (TYR) activity is in urgent demand for both fundamental research and practical application, but the exploration of functional materials with good biocompatibility for its activity evaluation at the intracellular level is still challenging until now. In this work, we develop a convenient and real-time assay with high sensitivity for TYR activity/level monitoring and its inhibitor screening based on biocompatible dopamine functionalized carbon quantum dots (Dopa-CQDs). Dopamine with redox property was functionalized on the surface of carbon quantum dots to construct a Dopa-CQDs conjugate with strong bluish green fluorescence. When the dopamine moiety in Dopa-CQDs conjugate was oxidized to a dopaquinone derivative under specific catalysis of TYR, an intraparticle photoinduced electron transfer (PET) process between CQDs and dopaquinone moiety took place, and then the fluorescence of the conjugate could be quenched simultaneously. Quantitative evaluation of TYR activity was established in terms of the relationship between fluorescence quenching efficiency and TYR activity. The assay covered a broad linear range of up to 800 U/L with a low detection limit of 7.0 U/L. Arbutin, a typical inhibitor of TYR, was chosen as an example to assess its function of inhibitor screening, and positive results were observed that fluorescence quenching extent of the probe was reduced in the presence of arbutin. It is also demonstrated that Dopa-CQD conjugate possesses excellent biocompatibility, and can sensitively monitor intracellular tyrosinase level in melanoma cells and intracellular pH changes in living cells, which provides great potential in application of TYR/pH-associated disease monitoring and medical diagnostics.
研究领域:
生物化学
·
医学诊断
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBON
·
2018
·
IF 7.082
Zhaolan Zhai,Xinyan Yan,Ji Xu,Zhanqian Song,Shibin Shang,Xiaoping Rao
The phase behavior and aggregates induced by changes in pH in a sodium N -tetradecyl-maleimidepimaric carboxylate (C 14 -MPA-Na)/cetyltrimethylammonium bromide (CTAB) system have been investigated using rheology and cryogenic transmission electron microscopy (cryo-TEM). Viscoelastic solutions at a pH between 6.40 and 1.41, opalescent blue solutions at a pH between 1.41 and 1.32 and aqueous surfactant two-phase systems (ASTPs) at a pH between 1.32 and 0.56 are formed sequentially. The microstructures in the viscoelastic solutions are wormlike micelles that partially transform to vesicles in the opalescent blue solutions. The aggregates in the upper phase of the ASTPs are vesicles and lamellar micelles, and in the lower phase are spherical micelles. Further, Single-walled carbon nanotubes (SWNTs) were successfully hybridized into the wormlike micellar viscoelastic solutions. cryo-TEM, UV–Vis-IR, near-infrared photoluminescence (NIR-PL) and UV–Vis spectroscopy were used to study the dispersion of SWNTs in the viscoelastic solutions, and the results showed SWNTs can remain dispersed for more than 6 months at pH 6.40. The results obtained from rheology showed that the hybridization of SWNTs will not affect the aggregates and viscoelasticity compared that to the native viscoelastic solution. Above more, the SWNTs could be recycled by the pH-switched phase behaviors between viscoelastic solutions and ASTPs.
研究领域:
材料科学
·
胶体化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2020
·
IF 0
Hongguang Liao,Yuting Zeng,Shenglong Liao,Yanji Chu,You Zhou,Yapei Wang
Percutaneous chemical ablation (PCA) is the oldest and most established technique for treating small solid tumors in organs. It has been widely used in clinics even on an outpatient basis. However, compared with the emerging microwave or magnetic hyperthermal ablation, PCA is faced with relatively poor necrosis results and needs to repeat multiple sessions. Inspired by the three effects in the bomb’s explosive process, we herein expect to combine calcium carbide (CaC2) nanoparticles into the PCA technique to generate local explosion within tumor tissues, leading to three killing effects against tumors to further improve the ablation efficacy of PCA. Through an efficient wet milling procedure with poly(ethylene glycol), three kinds of nanobombs including CaC2, calcium oxide (CaO), and calcium hydroxide (Ca(OH)2) were fabricated, and they all exhibited desirable suspension stability. Among these nanobombs, in particular CaC2 nanobombs showed a synergistic effect that the generation of ethyne gas bubbles could facilitate the most rapid diffusion of hyperthermia. Also, CaC2 nanobombs offered the powerful ability to cause the sudden rise of local high temperature and pH value. According to the in vivo mice tumor excision trial, the tumors of 75% of cases that received CaC2 treatment were destroyed and eradicated, exhibiting the excellent ablation ability of CaC2 nanobombs against small solid tumors planted in mice.
研究领域:
纳米技术
·
肿瘤学
·
生物医学工程
✓ 已验证
DOI
PubMed
Biomedical Optics Express
·
2022
·Vol. 13
·期 7
·4021-4031
·
IF 3.562
Jingjing Zhao,Jingjing Zhao,Shaohua Lu,Shaohua Lu,Julio Bastos-Arrieta,Julio Bastos-Arrieta,Cristina Palet,Yiling Sun,Renheng Wang,Zhengfang Qian,Shuting Fan
We presented a strategy for enhancing the sensitivity of terahertz glucose sensing with a hydrogel platform pre-embedded with Au nanoparticles. Physiological-level glucose solutions ranging from 0 to 0.8 mg/mL were measured and the extracted absorption coefficients can be clearly distinguished compared to traditional terahertz time domain spectroscopy performed directly on aqueous solutions. Further, Isotherm models were applied to successfully describe the relationship between the absorption coefficient and the glucose concentration (R2 = 0.9977). Finally, the origin of the sensitivity enhancement was investigated and verified to be the pH change induced by the catalysis of Au nanoparticles to glucose oxidation.
研究领域:
生物物理学
·
纳米技术
·
光谱学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）