Frontiers in Bioengineering and Biotechnology
·
2023
·
IF 5.7
Tang Ting,Gong Yufang,Gao Yuan,Pang Xinlong,Liu Shuangqing,Xia Yulan,Liu Dongsheng,Zhu Lin,Fan Qing,Sun Xiao
Introduction: The tumor microenvironment (TME) is mainly characterized by abnormally elevated intracellular redox levels and excessive oxidative stress. However, the balance of the TME is also very fragile and susceptible to be disturbed by external factors. Therefore, several researchers are now focusing on intervening in redox processes as a therapeutic strategy to treat tumors. Here, we have developed a liposomal drug delivery platform that can load a Pt(IV) prodrug (DSCP) and cinnamaldehyde (CA) into a pH-responsive liposome to enrich more drugs in the tumor region for better therapeutic efficacy through enhanced permeability and retention effect.Methods: Using the glutathione-depleting properties of DSCP together with the ROS-generating properties of cisplatin and CA, we synergistically altered ROS levels in the tumor microenvironment to damage tumor cells and achieve anti-tumor effects in vitro.Results: A liposome loaded with DSCP and CA was successfully established, and this liposome effectively increased the level of ROS in the tumor microenvironment and achieved effective killing of tumor cells in vitro.Conclusion: In this study, novel liposomal nanodrugs loaded with DSCP and CA provided a synergistic strategy between conventional chemotherapy and disruption of TME redox homeostasis, leading to a significant increase in antitumor effects in vitro.
研究领域:
药剂学
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肿瘤学
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纳米医学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2025
·
IF 5.2
Shiwen Gong,Wentao Ma,Die Hu,Haixing Xu,Binbin Li,Xinyu Wang
Inflammatory bowel disease ( IBD) is an idiopathic, lifelong, and devastating chronic inflammatory disease of the gastrointestinal tract. Berberine, an alkaloid extracted from the Chinese herb Huanglian, is a pharmaceutical ingredient with antibacterial, anti-inflammatory, and antiviral pharmacological effects in Chinese medicine. However, direct administration of berberine requires high doses due to its low bioavailability and high waste in the stomach. In this study, we prepared berberine-loaded pH-responsive Eudragit® FS 30D-attapulgite (FS-ATT) microspheres for the targeted treatment of colitis. The microspheres had pH-responsive release behavior, with minimal berberine release (about 5.97%) in simulated gastric fluid and high berberine release in simulated colonic fluid (about 69.27%). In addition, it was revealed by in vivo biological evaluations that, compared with drugs in an equivalent solution form taken orally, a significant improvement in IBD symptoms was observed after oral administration of drug-loaded FS-ATT microspheres, leading to an increase in the diversity of intestinal microbiota, inhibition of inflammatory cytokines, and enhanced therapeutic effects of berberine. The pH-responsive FS-ATT microspheres could offer a promising drug delivery platform for managing and treating many gastrointestinal diseases.
研究领域:
药剂学
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生物医学
·
胃肠病学
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DOI
PubMed
高影响因子
ACS Omega
·
2021
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IF 3.512
Guodong Zheng,Jiahui Zheng,Le Xiao,Tongyi Shang,Yanjun Cai,Yuwei Li,Yiming Xu,Xiaoming Chen,Yun Liu,Bin Yang
In this study, a pH-responsive nano-prodrug was fabricated by conjugating emodin to the PEGylated polyethyleneimine (mPEG-PEI) with acid-sensitive boronate ester bonds. 1H NMR spectra results showed that emodin was effectively bonded to mPEG-PEI, and acid-sensitive assay further confirmed the formation of boronate ester bonds. The size and morphology of the nano-prodrug were ascertained through transmission electron microscopy (TEM) and dynamic light scattering (DLS), which showed that the prodrug has a sphere-like shape with hydrodynamic size around 102 nm at pH 7.4. Subsequently, a drug-release behavior assay was carried out to carefully investigate the acid-sensitive drug-delivery property of the prodrug. Moreover, in vitro cell viability assay confirmed the superior cytotoxic effect of the nano-prodrug against HeLa cells compared to free emodin. Furthermore, the antibacterial study showed that the nano-prodrug could inhibit the bacterial (both Gram-positive and Gram-negative) growth more effectively than free emodin. Overall, this study provides a promising paradigm of the multifunctional nano-prodrug for pH-responsive tumor therapy and antibacterial activity.
研究领域:
药剂学
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肿瘤学
·
微生物学
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产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2022
·Vol. 14
·期 3
·535
·
IF 6.525
Huiru Zhu,Li Kong,Xu Zhu,Tingting Ran,Xiaojuan Ji
A high incidence of restenosis has been reported at the site of inflammation following angioplasty and stent implantation. The anti-proliferative drug paclitaxel (PTX) could help to reduce inflammation and restenosis; however, it has poor water solubility and serious adverse side effects at high doses. Given the presence of metabolic acidosis at the site of inflammation, we hypothesized that nanoparticles that are responsive to low pH could precisely release the loaded drug at the target site. We successfully constructed pH-responsive poly(D, L-lactic-co-glycolic acid) (PLGA) nanoparticles loaded with PTX and NaHCO3as a pH-sensitive therapeutic agent (PTX-NaHCO3-PLGA NPs). The NPs exhibited remarkable pH sensitivity and a good safety profile both in vitro in rat vascular smooth muscle cells and in vivo in Sprague Dawley rats after tail vein injection. In the rat model, the PTX-NaHCO3-PLGA NPs treatment group showed suppressed intimal proliferation following balloon-induced carotid artery injury compared with that of the saline-treated control. Overall, these results demonstrate that our newly developed pH-responsive nanodrug delivery platform has the potential to effectively inhibit restenosis.Keywords:vascular restenosis;pH-responsive nanoparticles;sodium bicarbonate;paclitaxel;drug delivery
研究领域:
医学
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药学
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生物材料
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Qing Sun,Jingsi Chen,Ziqian Zhao,Diling Yang,Yaoyu Xiao,Hao Zhang,Xin Ma,Hong Zhong,Hongbo Zeng
Stepwise separation of multi-species with a single type of chemical additives (such as surfactants) is an auspicious but challenging issue in many engineering processes as one surfactant usually lacks the ability to interact with different components. One promising strategy is to develop an environment-responsive surfactant, which possesses various functional groups to anchor target constituents under different environmental conditions. In this work, we have prepared a pH-responsive surfactant (2-(benzylthio)-acetohydroxamic acid, BTHA) possessing two pH-switchable groups (i.e., thioether and hydroxamate) that can selectively interact with different target substances, thus enabling stepwise and efficient separation of a three-component mineral system. The single in-house synthesized BTHA is successfully applied to the sequential separation of pyrite, diaspore, and kaolinite from high-sulfur bauxite by simply tuning the solution pH. Specifically, under the acidic condition (pH 6.0), the hydroxamate group of BTHA is deactivated, thus only the thioether groups can directionally interact with pyrite and enable the selective flotation recovery of pyrite to 85.9%. When the solution pH is subsequently increased to 8.0, the active hydroxamate group of BTHA can tightly chelate with diaspore and result in its efficient separation from kaolinite. The bifunctional surfactant exhibits strong and selective interactions with target substrates via pH modulation, allowing the adsorption selectivity and efficient stepwise separation, which provides an innovative paradigm for the development of novel surfactants with diverse applications in the mineral, metallurgical, chemical, and renewables recycling industry.
研究领域:
矿物加工工程
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化学工程
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
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IF 8.758
Kunlin Chen,Jianlin Zhou,Jing Hu,Junhao Zhang,Tianzuo Heng,Changyue Xu,Xuemei Wang,Jingyan Liu,Kejing Yu
Currently, smart and functional textiles have attracted increasing attention for the research on their application in various fields. In this paper, perfluorooctyltriethoxysilane (FAS13)-loaded silica nanocapsules taken as the Pickering emulsifier was applied to stabilize O/W emulsion for obtaining pH-responsive dual-compartmental microcapsules which show a strawberry-like structure with jasmine essence as the core and pH-responsive polymers and silica nanocapsules as the shell. These microcapsules could endow it with multifunctions by functionalizing the fabric, while the preparation and functionalization process is effortless and environmental friendly. Not only does the treated fabric demonstrate the self-healing superhydrophobicity and ultraviolet (UV) resistance because of the hydrophobic FAS13 getting loaded into silica nanocapsules and the surface modification of UV absorbent, it is also capable of the pH control jasmine essence-releasing performance, which allows over 40% of the fragrance to be preserved for three months through the controlled release of jasmine essence from the microcapsules.
研究领域:
材料科学
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纺织工程
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化学工程
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DOI
高影响因子
Journal of Biomedical Nanotechnology
·
2017
·Vol. 13
·期 12
·1647-1659
·
IF 4.521
Cao Jun,Zheng Haoran,Hu Rui,Liao Jianhong,Fei Zengming,Wei Xuan,Xiong Xiong,Zhang Fanglin,Zheng Hua,Li Dan
The drug controlled release responding to tumour microenvironment is a hotspot for cancer therapy research. Herein, pH-sensitive shell-core nanoparticles (NPs) were devised and fabricated for tumour treatment. The detailed characterisations of their structures demonstrated the successful
formation of the precursors and NPs. More importantly, the NPs show excellent features, i.e., negative surface potential, narrow size distribution, and suitable dimension for cell penetration. And they have superior stability in pH 7.4 buffer (i.e., blood environment). In addition, owing to
the existence of the pH-labile imine bond, a controlled release of drugs from NPs was achieved in drug release test. Confocal laser scanning microscopy (CLSM) and flow cytometry proved the internalization of NPs into HeLa cells and their further translocation into cell nuclei. The cytotoxicity
results also revealed a remarkable cytotoxicity of the NPs to HeLa cells. Thus, this type of NP may be potentially useful for the efficient drug delivery.
研究领域:
药剂学
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肿瘤学
·
生物材料学
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
·
IF 8.6
Yuxiao Hu,Xueying Chen,Zhijian Tan
Ammonium sulfate and t -butanol are widely used in conventional three-phase partitioning (TPP); however, their use is not eco-friendly. In this study, a novel TPP was developed using pH-responsive deep eutectic solvents (DESs) and sugars as substitutes for t -butanol and ammonium sulfate, respectively. This TPP was further used for the separation and purification of radish ( Raphanus sativus L.) peroxidase (POD). DES-3 (dodecanoic acid: decanoic acid = 1:1) and sucrose were selected as optimal phase-forming components. Single-factor experiments were performed to investigate the factors influencing the TPP process, and three major factors (sucrose concentration, extraction time, and extraction temperature) were optimized by response surface methodology (RSM). Under the optimized conditions, the POD recovery and purification fold reached 101.66% and 11.56, respectively. Cyclic experiments confirmed the retrievability of DESs. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed that the novel TPP had better purification efficiency than the traditional TPP. The method proposed in this study is more efficient than conventional TPP, and it does not require the use of organic solvents and high concentration salt solutions. Therefore, this method is promising in the application of TPP.
研究领域:
生物化学
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生物技术
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环境科学
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产品关联
DOI
高影响因子
Separations
·
2023
·Vol. 10
·期 10
·523
·
IF 2.6
Xiangfen Cui,Jingmei Liao,Huaying Liu,Wei Tang,Cheng Tie,Senlin Tian,Yingjie Li
The presence of organic pollutants in wastewater remains a prominent environmental concern due to the related ecological and health hazards. In response, this study employs an adsorptive methodology to address the removal of phenol and catechol, utilizing an organo-bentonite material modified with a pH-responsive switchable surfactant, dodecyldimethylamine oxide (C12DAO). The synthesized organo-bentonite (C12DAO-Bt) manifests commendable thermostability resulting from thermogravimetric analyses. The adsorption capacities of C12DAO-Bt concerning phenol and catechol intensify with the augmentation of the C12DAO/bentonite mass ratio. The utmost adsorption capacities of 150C12DAO-Bt, deduced through a pseudo-second-order kinetic model, stand at 5.72 mg·g−1for phenol and 5.55 mg·g−1for catechol, respectively. Subject to modification by a pH-responsive surfactant, conditions leaning towards weakly acidic and neutral conditions (pH = 6~7) are conducive to the adsorption of phenolic compounds. Conversely, alkaline conditions (pH = 8~9) facilitate the dissociation of adsorbates from adsorbents. The augmentation of cationic strength within the examined scope incites the adsorption procedure while impeding the desorption efficacy. In the case of cationic species with comparable ionic strengths, Na+exhibited a superior effect on the adsorption–desorption dynamics of phenol, while Ca2+exerts a more pronounced effect on those of catechol. Moreover, even following five consecutive acid–base regulation cycles, C12DAO-Bt retains a relatively high adsorption capacity and desorption efficacy, which underscores its exceptional regenerative capacity for removing phenolic compounds from wastewater.Keywords:pH-responsive surfactants;organic bentonite;reversible adsorption;phenolic pollutants;wastewater
研究领域:
环境科学
·
化学工程
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产品关联
DOI
高影响因子
Journal of Magnesium and Alloys
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2020
·
IF 7.115
Yuejun Ouyang,Lin-Xin Li,Zhi-Hui Xie,Lili Tang,Fuhui Wang,Chuan-Jian Zhong
The preparation of pH-responsive nanocontainers by typical silane modification of the mesoporous silica nanoparticle (MSN) surface is usually high-cost, complex, and time-consuming, which remains a great challenge for effective corrosion protection of magnesium alloy. Here, a new strategy to construct pH-responsive nanocontainers (MSN-MBT@LDH) is demonstrated. The nanocontainers consist of corrosion inhibitor (2-mercaptobenzothiazole, MBT) loaded MSN core and layered double hydroxide (LDH) nanosheet shell serving as gatekeepers. The successful loading of MBT and encapsulation by LDH nanosheets were confirmed by a series of characterization such as scanning transmission electron microscopy coupled with energy dispersive X-ray spectroscopy (STEM-EDS) and N 2 adsorption/desorption isotherms. The pH-responsive feature of the nanocontainers was demonstrated by determination of the MBT concentration in buffer solutions with different pH values. A smart corrosion protection system on Mg alloy is obtained by incorporating the synthesized nanocontainers into a self-assembled nanophase particle (SNAP) coating. The electrochemical tests and visual observations show that the hybrid coating has the best barrier properties and robustness in corrosion protection in NaCl corrosive solutions in comparison with the control coatings. The present method simplifies the synthesis processes of nanocontainers and eliminates the potential detrimental effect of excess gatekeepers on the coating. The findings provide new insights into the preparation of scalable nanocontainers. The self-healing coatings are expected to have widespread applications for corrosion protection of Mg alloy and other metals.
研究领域:
材料科学
·
腐蚀防护
·
纳米技术
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DOI
高影响因子
Journal of Materials Chemistry B
·
2013
·Vol. 1
·期 25
·3202-3208
·
IF 0
Peng Fei Gao,Lin Ling Zheng,Li Jiao Liang,Xiao Xi Yang,Yuan Fang Li,Cheng Zhi Huang
A new type of coordination polymer sphere prepared by combining 1,1′-(1,4-butanediyl)bis(imidazole) (bbi) and ferrous ions has been demonstrated as a targeted delivery system for in situ encapsulating anticancer drugs. These stable coordination polymer spheres can be fabricated simply by a deposition method. Drugs, doxorubicin hydrochloride (DOX·HCl) for example, can be easily in situ encapsulated by simply mixing the drug with bbi ligand through the deposition method and results in a high drug loading efficiency up to 98% and a drug loading content of nearly 40%, which is remarkably high for not only metal–organic but also other materials. A noticeable feature of the drug loaded coordination polymer spheres is that they show sustainable drug release for several days due to their superior stability, and are sensitive to external pH owing to the coordination bonds. The drug can be released faster in mild acidic conditions in comparison to physiological acidity. By conjugating folic acid to the surface of the coordination polymer spheres, the vehicles can be taken up selectively by cancer cells through cell surface receptor-mediated mechanisms. Cell viability experiments with HeLla cells demonstrated the low toxicity of the delivery vehicles and the good anticancer efficacy of the drug-loaded coordination polymer spheres.
研究领域:
药剂学
·
生物材料学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
JOURNAL OF MOLECULAR LIQUIDS
·
2024
·
IF 5.3
Jia Li,Ming Wen,Lei Lei,Cheng Fu,Zeyin Jiang
In this study, a novel surfactant with pH-responsive properties was developed to address the challenges associated with balancing foaming and defoaming properties in traditional foam drainage gas recovery processes. The surfactant was created by employing primary amine and aldehyde monomers based on the principles of reversible dynamic covalent bonds. Optimal synthesis conditions were determined through an extensive analysis of foaming performance: a molar reaction ratio of primary amine to aldehyde at 10:0.8, a 20-minute reaction time, and a stirring speed of 300 r/min. The formation of dynamic imine bonds was confirmed through infrared spectroscopy. Surface tension measurements revealed that at pH = 8, the surfactant achieved a minimum surface tension of 31.1 mN/m, while at pH = 3, the surface tension exceeded 40 mN/m. Particle size analysis demonstrated a 26.5-fold reduction in particle size as the pH decreased from 8 to 3. Zeta potential tests indicated excellent dispersion stability within the pH range of 3–8, with Zeta potential consistently exceeding 25 mV. Furthermore, the surfactant displayed remarkable foaming and foam stabilizing effects at pH = 8, with initial and 5-minute foam heights reaching 168 mm and 132 mm, respectively. When the pH dropped below 6, the surfactant exhibited rapid defoaming. After six cycles of alternating acid-base environments, it maintained excellent foaming performance, achieving a 5-minute foam height of up to 138 mm. Importantly, it retained its foaming capabilities in formation water solutions with a mineralization degree of 200 g/L. These findings hold significant implications for the development of intelligent environmental response treatments for foam drainage and gas production, enhancing the overall efficiency of drainage and gas production operations.
研究领域:
化学工程
·
石油工程
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）