STRUCTURE
·
2024
·
IF 4.4
Jian Sun,Xiangxiang Zhang,Liu Xue,Liang Cheng,Jing Zhang,Xin Chen,Zhirong Shen,Kang Li,Lai Wang,Chichi Huang,Jing Song
TIGIT is mainly expressed on T cells and is an inhibitory checkpoint receptor that binds to its ligand PVR in the tumor microenvironment. Anti-TIGIT monoclonal antibodies (mAbs) such as Ociperlimab and Tiragolumab block the TIGIT-PVR interaction and are in clinical development. However, the molecular blockade mechanism of these mAbs remains elusive. Here, we report the crystal structures of TIGIT in complex with Ociperlimab_Fab and Tiragolumab_Fab revealing that both mAbs bind TIGIT with a large steric clash with PVR. Furthermore, several critical epitopic residues are identified. Interestingly, the binding affinity of Ociperlimab toward TIGIT increases approximately 17-fold when lowering the pH from 7.4 to 6.0. Our structure shows a strong electrostatic interaction between ASP103 HCDR3 and HIS76 TIGIT explaining the pH-responsive mechanism of Ociperlimab. In contrast, Tiragolumab does not show an acidic pH-dependent binding enhancement. Our results provide valuable information that could help to improve the efficacy of therapeutic antibodies for cancer treatment.
研究领域:
免疫学
·
结构生物学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
NANOTECHNOLOGY
·
2022
·Vol. 33
·期 26
·265002
·
IF 3.953
Xiaohui Fan,Yang Wang,Bo Li,Chang Shen,Zhengguang Sun,Yuan Zhan,Yuhong Zhang
Carbon quantum dots (CDs) have attracted tremendous interest owing to their idiosyncratic functions and wide-ranging applications. However, it remains a great challenge to empolder an integrated CDs combining high luminescence, biocompatibility and luminescence color tunability for bioimaging via simple approach. In this work, pH-responsive carbon quantum dots (Si-CDs) with high luminescence (quantum yield = 74.8%) were fabricated by one-step hydrothermal method using (3-mercaptopropyl) triethoxysilane (KH-580) as modifier for the first time. The optical properties of the as-prepared Si-CDs can be controlled from obvious green–blue–violet transformation by altering the pH. More importantly, the change is reversible and repeatable. In addition, the Si-CDs have good biocompatibility and chemically inert in vitro cell system simulation. Such non-toxic, environmental friendly, low-cost, inert CDs materials are promising candidates for biomedical and pH-sensitive sensors.
研究领域:
生物医学
·
材料科学
·
光学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Nano Materials
·
2022
·
IF 6.14
Seojin Jung,Seokhee Chang,Na-Eun Kim,Seong-O Choi,Yoon-Jae Song,Yue Yuan,Jooyoun Kim
A feasible strategy of on-demand drug delivery for the treatment of dermal inflammation under low-pH conditions is proposed, employing zeolitic imidazolate framework-8 (ZIF-8) as a pH-responsive nanoparticle and curcumin (CCM) as a model drug. To overcome the low bioavailability of topically treated drug, a microneedle (MN) form is used to incorporate CCM and ZIF-8. Taking advantage of the fact that ZIF-8 degrades under acidic conditions, CCM is embedded in porous ZIF-8 nanoparticles such that CCM is released when ZIF-8 comes into contact with an acidic dermal fluid at the inflammation site, and this CCM-encapsulated ZIF-8 (CCMZIF) is incorporated into water-dissolvable poly(vinyl pyrrolidone) MN. The ZIF-8 shows a high loading capacity (∼40.5%) of CCM through chemical bonding and physical adsorption. From in vitro tests with both a buffered solution and porcine skin, CCM from the CCMZIF MN is released in a higher amount at pH 5.0 than at pH 7.4, demonstrating the capability of the pH-responsive release of the drug when needed at inflammatory sites. The analytical investigation conducted here reveals that an acidic environment triggers the structural degradation of ZIF-8, allowing the release of the chemically bonded CCM. Cytotoxicity and stability tests demonstrate the good biocompatibility and bioavailability of ZIF-8. This study highlights the analytical discussion of the encapsulation and release mechanism of CCM in a ZIF-8-implemented MN drug delivery platform. The results demonstrate an advanced on-demand therapeutic strategy for skin disorder treatment.
研究领域:
药剂学
·
生物材料学
·
皮肤病学
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DOI
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2016
·Vol. 16
·期 6
·5689-5695
·
IF 1.338
Wu Jindan,Jiang Yuelin,Cai Guoqiang,Wang Zhengliao,He Ju,Wang Jiping
With the development of material science, pH-responsive fabric emerges as a novel material in recent years. Effect of chain density and chain length on the conformation transition of surfaceanchored polymers is a fundamental problem which relates to their functionality. In this work,
poly(acrylic acid) (PAA) was grafted onto cotton fabrics through surface-initiated atom transfer radical polymerization (ATRP) technique. The chain length of PAA was modulated by monomer concentration, and the grafting density was regulated by the density of amino groups on the fabric surface.
Contact angle analysis, IR measurement and water uptake observation were employed to study the pH-responsive behavior of the PAA modified cotton. Fabrics with polymers of a larger molecular weight and optimal grafting density exhibited better pH-responsibility. This study provided a structure-property
fundamental understanding on the important parameters influencing the pH-responsibility of PAA modified cotton fabric.
研究领域:
材料科学
·
高分子化学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2019
·Vol. 7
·期 4
·1643-1651
·
IF 5.251
Zhouxiang Zhao,Chunmei Ding,Yaqin Wang,Hong Tan,Jianshu Li
Traditional antibacterial treatments, such as chlorhexidine (CHX), destroy cariogenic biofilms. However, they exert negative effects in clinical applications, for example, teeth staining, taste disturbance and harm to oral tissue after a long-term exposure. Therefore, biocompatible strategies for efficient antibacterial drug delivery are in high demand. In this study, aimed at dental caries therapy enhancement, we designed a pH-responsive nanocarrier system, capable of releasing CHX in an acidic environment within cariogenic biofilms. Cationic poly(ethylene glycol)-block-poly(2-(((2-aminoethyl)carbamoyl)oxy)ethyl methacrylate) (PEG-b-PAECOEMA) was synthesized first. Modification of PAECOEMA by citraconic anhydride (CA) forms negatively charged PEG-b-PAECOEMA/CA, which could assemble into core–shell polyionic complex micelles (PICMs) when mixed with cationic CHX via electrostatic interactions. PICMs are stable in healthy neutral oral microenvironments with CHX encapsulated in the core and PEG shell exposed. Once in acidic milieu within caries-producing biofilms, they rapidly disassemble and release CHX cargo owing to degradation of citraconic amide groups. Molecular structures of the above copolymers were confirmed using 1H NMR and gel permeation chromatography (GPC) analysis. The pH-dependent degradation rates of citraconic amide in PEG-b-PAECOEMA/CA copolymer were measured by fluorescamine method. Atomic force microscopy (AFM) studies confirmed successful assembly of well-defined spherical PICMs in aqueous solution. The disassembly of PICMs in acidic microenvironment was observed using dynamic light scattering (DLS). PICMs showed an obvious pH-dependent drug release profile when the pH changed from 7.4 to 5.5. More importantly, the micellar system could reduce drug toxicity of CHX and exhibited outstanding antibacterial capability in the biofilm of Streptococcus mutans. Micelles constructed from pH-sensitive PEG-b-PAECOEMA/CA are highly promising for dental caries therapy and provide guidelines for drug-delivery system design in other acidic pathologic systems.
研究领域:
牙科学
·
材料科学
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Lanlan Gan,Qian Su,Zhangbao Chen,Xiaoming Yang
Detections of carcinogen and vitamins are generally recognized to be meaningful, especially for nitrites (NO 2 − ) and ascorbic acid (AA). Here blue-fluorescence carbon dots (CDs) with the quantum yield of 11.83% were successfully synthesized by using folic acid and nicotinic acid, and their surfaces were identified to be abundant in the functional groups of amide, carboxyl and hydroxyl. Importantly, the amide on the surfaces of CDs showed the interactions with the nitrites and formed the complex, thus resulting in their quenched fluorescence. To be specific, the fluorescence intensity of CDs proportionally decreased by adding the increased amounts of NO 2 − from 8 to 800 μM, and achieved the detection of NO 2 − at a detection limit of 21.2 µM. Moreover, further introducing ascorbic acid could prevent CDs with NO 2 − to form the complex and recover their fluorescence, and thus providing the new avenue of assaying AA with the generally favorable linear range of 0.1–800 μM, while its related detection limit was 50 µM. In addition, the synthesized CDs exhibited the satisfactory sensitivity toward different pH values, demonstrating their potential of sensing pH. Consequently, the proposed CDs here may provide a new way of monitoring NO 2 − and AA in the target samples.
研究领域:
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 22
·5330
·
IF 4.2
Shuzhen Yang,Fulin Lü,Li Wang,Sinan Liu,Zhisai Wu,Yanqin Cheng,Fan Liu
A smart and environmentally friendly pesticide system was developed that could respond to environmental stimuli while mitigating environmental risks. In this study, thiabendazole (Thi), an effective fungicide, was loaded onto zeolitic imidazolate framework-8 (ZIF-8) using the impregnation method to fabricate a pH-responsive nano hybrid delivery system (Thi@ZIF-8). The results demonstrated that Thi@ZIF-8 had a rhombic dodecahedral morphology and a loading capacity of approximately 25%. Notably, the amount of Thi released from Thi@ZIF-8 at a pH of 5.0 reached 79.54%, which was higher than that at pH 7.0 and 9.0, for 251 h. Such pH-responsive release characteristics of Thi@ZIF-8 were probably related to the pH-dependent structure stability of ZIF-8. The release mechanism of Thi@ZIF-8 conformed to non-Fickian diffusion. Additionally, Thi@ZIF-8 showed a higher control efficacy againstB. cinereacompared with Thi alone. Importantly, the ZIF-8 carrier could effectively reduce the leaching loss of Thi in soil and showed no negative effects on the three varieties of tomato seedlings, implying good biocompatibility. This work provides a novel and eco-friendly approach to control B. cinerea effectively that has great potential in modern sustainable agriculture.
研究领域:
农业科学
·
材料科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Polymer Materials
·
2024
·
IF 4.4
Zhonghua Lv,Xi Chen,Ling Yang,Mengting Li,Changjun Xia,Chunjia Luo,Min Chao,Luke Yan
We present a biomimetic switchable MXene membrane for water treatment inspired by chloride ion transmembrane transport. The MXene membrane was modified with poly(2-diethylaminoethyl methacrylate), resulting in increased hydrophilicity and enlarged channels. The membrane exhibited a permeability of 4.42 × 104 L m–2 h–1 bar–1, surpassing conventional MXene membranes. The membrane also separated Methylene Blue positively charged at pH = 12 and Amido Black 10B negatively charged at pH = 2 with separation efficiency above 98%, thanks to controllable channel size and adjustable electrostatic interactions in response to pH or CO2. This research offers valuable insights into the design of pH-responsive membranes with adjustable selectivity, representing a significant advancement in smart filtration with versatile applications.
研究领域:
材料科学
·
环境工程
·
生物仿生学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
·
2025
·
IF 2.4
Genpei Zhang,Xilong Zhang,Kai Yue,Weishen Zhong
Liposomes serving as nanocarriers offer significant advantages in drug delivery for tumor treatment. There still exists challenges in controlling drug release by disintegrating the liposome membrane for the improvement of therapeutic efficiency. In this paper, a novel method involving the mixture of short peptides with pH-responsive characteristics into the cargo has been introduced. This approach facilitates the release of doxorubicin (DOX) in the acidic tumor tissue environment. The efficacy of this improvement was elucidated through molecular dynamics simulations and experiments. Liposomes incorporating a 1:1 ratio of peptides–DOX exhibited pronounced pH sensitivity and an enhanced drug release profile. The underlying mechanism is attributed to the peptides entering tumor tissues and undergoing protonation in acidic conditions, which increases the hydrophilicity of the peptide–DOX clusters and the internal surface tension of the liposomes. This alteration disrupts the balance between the inner and outer surface tensions of the nanocarrier, causing the liposomes to structurally disintegrate and thus enhancing drug release. The results from both thermodynamic analysis results and experimental data confirm the augmented drug release efficiency of this method, offering valuable theoretical insights for nanoparticle design and determining the optimal mixing ratio for therapeutic applications.
CAS / MDL:
研究领域:
药剂学
·
生物医学工程
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2025
·
IF 14.1
Hui Li,Fengzhen Meng,Chengwei Hu,Zhiyun Wu,Liuzhi Hao,Caijun Sun,Lijing Fang,Fan Pan,Shaoquan Bian,Huipeng Li,Mingjun Li,Bo Liu,Xiaoli Zhao
The microneedle, a minimally invasive transdermal system, provides a convenient and painless method for drug delivery. Among the various types of microneedles, hydrogel-forming microneedles (HFMs) demonstrate distinct advantages in terms of high-dose drug loading and biocompatibility. However, HFMs usually require drying to obtain sufficient puncture strength, which may destroy drug activity and increase storage costs. Herein, a high-strength HFM patch with pH-responsiveness for post-drug loading and long-term release is developed based on acrylonitrile-acrylic acid copolymer. The dipole-dipole and hydrogen bonding interactions formed through gradient solvent replacement are evenly distributed within the cross-linked network, significantly enhancing the mechanical properties of the hydrogel required for epidermal penetration. The prepared hydrogel exhibits a tensile strength of 26 MPa and a Young's modulus of 407 MPa. The microneedles formed from this hydrogel display a single needle mechanical force of 1.18 N. The post-loading mode conferred by pH responsiveness allows the drug to be encapsulated in both the tips and the substrate, acting as a reservoir. Once applied to the skin, the microneedle is activated by body fluids to achieve long-term drug release. Overall, this high-strength HFM improves the mechanical properties in the hydrated state, making it a promising minimally invasive transdermal delivery platform.
研究领域:
药剂学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2012
·
IF 1.58
Yi-Tian Tang,Xiao-Qiu Dou,Zhi-Ang Ji,Ping Li,Shen-Min Zhu,Jia-Jun Gu,Chuan-Liang Feng,Di Zhang
Dye/water separation is a worldwide challenge because of the increasing industrial dye wastewater. In this paper, a novel pH-responsive C 2 -symmetric cyclohexane based hydrogel was developed and was capable of rapidly adsorbing dye molecules in minutes. This hydrogel dye adsorbent was characterized by Fourier transform infrared (FT-IR) spectroscopy, Ultraviolet–visible (UV–vis) spectroscopy, Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The adsorption equilibrium of the dyes is in good agreement with Langmuir adsorption isotherm model. Moreover, the hydrogel adsorbent can be recovered easily just by changing the pH of the medium, which is promising to be used an efficient dye adsorbent in water purification .
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
AUSTRALIAN JOURNAL OF CHEMISTRY
·
2013
·Vol. 67
·期 1
·134-141
·
IF 1.869
Yiheng Wang,Mengge Xia,Yongtao Wu,Meifang Zhu
Functionalized magnetic microspheres were prepared by anchoring cross-linked core–shell poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-co-AA)) nanoparticles onto silica-coated magnetic microspheres (Fe3O4@SiO2). First, the smaller polystyrene/P(NIPAM-co-AA) core–shell nanoparticles were synthesized through seed emulsion polymerization and adhered to the surface of amino-modified Fe3O4@SiO2 micorspheres, which were made using the modified Stöber method through electrostatic interaction under appropriate preparation conditions. An amidation reaction between the carboxylic and amino groups on the respective surfaces was then catalyzed. Finally, the novel architecture magnetic microspheres with multiresponsive functionalities were obtained, and their polymerization conditions, environmental sensitivity, and magnetic properties were discussed and optimized. The superparamagnetism and temperature/pH dual responsivity and excellent dispersibility of the P(NIPAM-co-AA) functionalized magnetic microspheres provide them with high potential to be used in the fields of controlled drug delivery, bioseparation, and catalysis.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）