Nanoscale
·
2018
·Vol. 10
·期 30
·14705-14711
·
IF 7.233
Qian Qian Zhang,Tong Yang,Rong Sheng Li,Hong Yan Zou,Yuan Fang Li,Jian Guo,Xi Dong Liu,Cheng Zhi Huang
Lysosomes, which can be easily targeted by molecules with abundant amino groups, play critical roles in endocytosis, autophagy, and phagocytosis; thus, it is important to accurately characterize lysosomes, including lysosomal pH, in living cells to understand their physiological and pathological functions. Herein, a new type of highly photoluminescent (PL) emerald carbon dots (CDs) was easily prepared through a functional preservation strategy (FPS) by simply mixing p-benzoquinone and ethanediamine at room temperature. The as-prepared CDs possessed abundant amino groups preserved from ethanediamine owing to FPS, and they exhibited excellent photostability as compared to the commercial LysoTracker probes. Consequently, they actively targeted lysosomes to sensitively respond to lysosomal pH in vitro owing to their abundant amino groups and good hydrophilicity. Thus, we could successfully monitor lysosomal pH dynamics during apoptosis in live cells.
研究领域:
细胞生物学
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生物医学工程
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分析化学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Lingling Li,Yang Li,Yijing Dang,Tingting Chen,Ao Zhang,Chunyong Ding,Zhiai Xu
Intracellular pH is a key physiological factor for controlling the activities and functions of cells and lysosome is a vital subcellular organelle. Thus, developing a novel lysosome-targeting fluorescence probe for selective and sensitive detection of lysosomal pH in living cells is very important. In this work, we synthesized a series of fluorescence probes based on imidazole-fused benzothiadiazole . The optical properties of these probes were easily adjusted by modifying the substituents with different electron-withdrawing/donating ability in imidazole moiety. All of them showed acid-response and decreased fluorescence intensity during pH values changing from 4.0 to 8.5. The introduction of morpholine group allowed them to specifically respond to the changes of lysosomal pH. Among them, probe MIBTAA possessed a suitable p K a value (5.3) and showed good linear response to pH (R 2 = 0.9918) with red emission when pH changed from 4.4 to 5.6. The probe was successfully applied for monitoring pH variation in living cells induced by proton-pump inhibitor Baf-A1 and chloroquine , indicating its great potential for pH imaging in biological applications.
研究领域:
生物化学
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细胞生物学
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医学影像学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Guobin Mao,Guoqiang Wu,Minghai Chen,Chuang Yan,Jingya Tang,Yingxin Ma,Xian-En Zhang
Intracellular pH is an important regulator of cell function, and its subtle changes may greatly affect cell activities and cause diseases. Reliable imaging of intracellular pH remains a huge challenge. Dual-emitting Mn2+-doped quantum dots (QDs) can be directly used as a ratiometric fluorescent probe without further modification, but they displayed low performance in terms of photoluminescence, stability, and intensity ratio regulation. Here, we report intrinsic dual-emitting CdZnSe/Mn:ZnS QDs with high photoluminescence efficiency, good stability, and biocompatibility. The emission intensity ratio was selectively regulated by Mn2+ doping. Because of aggregation-induced quenching of QDs, the exciton emission of CdZnSe/Mn:ZnS QDs (471 nm) was sensitive to pH, while the Mn2+-doped emission (606 nm) was passive to pH, which was probably due to little self-quenching in Mn2+-doped emission caused by weak Mn–Mn coupling interaction. Dual-emitting CdZnSe/Mn:ZnS QDs exhibited excellent pH-responsiveness in the range of pH 4.0 to 12.0 and were used for pH imaging in live HeLa cells. When the pH value of HeLa cells changed from 5.0 to 9.0, the emission changed from red to blue. Furthermore, these dual-emitting CdZnSe/Mn:ZnS QDs can provide a versatile platform for biosensors and biological imaging.
研究领域:
生物传感
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生物成像
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材料科学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yutao Jia,Yanting Shen,Yanyan Zhu,Jing Wang
Lysosomes are the acidic organelles in the cells that play an important role in intracellular degradation and other various cellular functions. The pH disturbance of lysosomes will result in the lysosomal dysfunction and many lysosomal related diseases. In this work, we reported a methoxy-based covalent organic framework (TAPB-DMTP-COF) that a novel pH-responsive fluorescent probe for lysosomal pH imaging in cells. The prepared TAPB-DMTP-COF presented regular crystal structure, low toxicity and good pH responsive property. The rich imine structure in the material enabled pH-responsive properties of the TAPB-DMTP-COF and made it exhibited pH-dependent fluorescence response. Good detection linearity for pH measurements in aqueous solution was achieved by this probe. Moreover, the TAPB-DMTP-COF can be used for the selective lysosomal pH imaging. Confocal fluorescence imaging results demonstrated that the pH fluctuations (from 4.0 to 7.4) and the pH changes in lysosomes can be effectively monitored in situ by the developed probe. This study may provide a new avenue for the intracellular pH sensing, deep study and understanding about the mechanism of diseases related to abnormal lysosomal pH.
研究领域:
生物化学
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材料科学
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医学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
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2022
·
IF 1.193
Xiao-Ping CHEN,Ya-Feng ZHUANG,Jian-Wei LIN,Jing-Hua CHEN,Zhi-Zhong HAN
Using citric acid and thiourea as precursors, sulfur-nitrogen co-doped dual emission carbon dots (DECDs) were prepared by a simple solvothermal method. At the excitation wavelength of 360 nm, DECDs have two emission peaks located at 465 nm (blue) and 592 nm (yellow-green) which are attributed by core and surface of DECDs. It is found that Fe 3+ ions can coordinate with oxygen-containing groups on DECDs, and then the electrons transfer from DECDs to Fe 3+ ions, which quench the fluorescence of DECDs. The fluorescence intensity is linear with the concentration of Fe 3+ ions in the range of 0–100 μM and the detection limit is 0.77 μM. Moreover, the designed sensor can be applied to detect Fe 3+ ions in tap water with high selectivity, which provides a basis for the quantitative detection of Fe 3+ ions. At the same time, DECDs can be used as a pH fluorescence probe. In the pH 2.5–11.5 range, the fluorescence increases with pH whilst the solution color changes, providing a visualization platform for pH detection. Moreover, the pH fluorescence probe can be used for intracellular pH fluorescence imaging in living cells, and DECDs have an excellent biocompatibility (e.g., cell viability remains about 80% after treatment for 24 h at 200 μg·mL −1 of DECDs). Therefore, DECDs has a certain application prospect in food detection, biological detection and determination of intracellular pH change.
研究领域:
化学
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生物医学工程
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分析化学
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DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Yingying Zhang,Yanliang Zhao,Bo Song,Chaobo Huang
Acidic pH is an important physiological parameter in environment and biological systems. Thus, designing a probe ( Hcy-F ) with good characters to monitor acidic pH is really important. Herein, a small molecular water-soluble probe is designed based on indole with high selectivity and sensitivity for detection the acidic pH in aqueous solution and living cells. When pH variation of aqueous is detected by probe, an obvious ratiometric and good linear response behavior are found in UV–vis absorption spectrum (R 2 = 0.9973, A 434 nm / A 371 nm = 6.9929–4.2231 pH) and fluorescence emission spectrum (R 2 = 0.9987, I 571 nm / I 514 nm = 7.2935–2.981 pH), which further proved the feasibility of Hcy-F to monitor the change of acidic pH in aqueous, respectively. Furthermore, Hcy-F is successfully applied for monitoring pH variation in living cells in dual channels (green and red) with higher cell survival rate, indicating the probe has great potential for pH imaging in biological applications .
研究领域:
分析化学
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生物化学
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细胞生物学
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Chunmin Qiu,Guoqiang Wu,Puying Zhao,Junbiao Dai,Qian Luo,Liqing Zhao,Guobin Mao,Yingxin Ma
Background High-precision and broad-range pH detection is critical for health status assessment, such as signal transduction, enzyme activity, endocytosis, and cell proliferation and apoptosis. Although pH-responsive ratiometric fluorescent probes offer an effective pH monitoring strategy, their preparation often requires multi-step modification and decreases fluorescence efficiency and stability. Herein, we developed a simple method to prepare fluorescent Si dots with dual emission centers for high-precision and broad-range pH monitoring, and the detection of urease based on pH-responsive Si dots and pH monitoring in living cell was further explored. Results The dual-emitting Si dots had two emission centers at 427 nm and 500 nm. The emission at 427 nm showed a fluorescence enhancement and effective quenching in the pH ranges of 3.0-10.0 and 10.0-12.0, respectively. While the emission at 500 nm increased gradually with increasing pH from 3.0 to 12.0. Two linear relationships were obtained between the synchronous fluorescence intensity ratio and pH in the ranges of 7.0-9.5 and 10.0-12.0 with a △pH of 0.1, indicating a broad detection range and high precision. Then the Si dots were used to detect urease activity via urea hydrolysis-mediated pH changes. A linear range of 1-80 U/L was established with a detection limit of 0.28 U/L. Furthermore, the Si dots were used for pH imaging in living HeLa cells. The cells changed from green to blue when the pH of HeLa cells increased from 6.0 to 10.0. Significance These findings collectively suggest that the intrinsic dual-emitting Si dots may offer a simple and versatile platform for developing pH-relevant biosensors and bioimaging applications. Additionally, this approach provides reliable methods for preparing intrinsic dual-emitting probe and constructing fluorescence ratiometrics, which widely used in health status assessment.
研究领域:
分析化学
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生物化学
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生物物理学
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生物传感器
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yan Xu,Weitao Yang,Zhuoyao Wu,Hui Wang,Tianming Cui,Weiwei Zeng,Yanjing Yun,Bingbo Zhang
Effective monitoring of gastric fluid pH is crucial for diagnosing gastric diseases and evaluating anti-gastric-acid therapies. The invasiveness and low patient compliance with traditional gastric fluid aspiration methods highlight the need of a non-invasive, real-time technique for in situ gastric pH monitoring. This study introduces a novel chromium (Cr)-based magnetic resonance probe, Cr(OH) 3 @HSA, specifically designed for pH-sensitive gastric imaging. Synthesized via an albumin-mediated biomimetic mineralization process, Cr(OH) 3 @HSA possesses an amorphous structure that facilitates the sensitive release of paramagnetic Cr(III) ions in acidic environments. This release substantially decreases the longitudinal relaxation time of surrounding hydrogen protons, as demonstrated by an 18.29-fold increase in the longitudinal relaxation rate from pH 6.5 to pH 1.0. In vivo experiments show that Cr(OH) 3 @HSA accurately detects abnormal gastric pH levels and evaluates the efficacy of gastric antiacids, surpassing the performance of the conventional “always-on” contrast agent, Magnevist®. Notably, our research reveals that chromium picolinate, a common dietary supplement for diabetes, generates a “turn-on” signal in the gastrointestinal tract, suggesting potential MR signal interference in diabetic patients. Consequently, we recommend that diabetic patients avoid chromium-based dietary supplements prior to MRI procedures to prevent Cr(III)-induced signal alterations.
研究领域:
医学影像
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胃肠病学
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生物医学工程
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材料科学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Huangmei Zhou,Yu Zhao,Xihang Chen,Sanjun Zhang
pH is a fundamental physiological parameter related to myriad applications, therefore great efforts are continuously made to develop high performance pH indicators. Here, we exhaustively investigated the pH response of an acridine analog, 9-acridinecarboxylic acid with steady state absorption and fluorescence spectroscopies, picosecond resolved fluorescence spectroscopy, and femtosecond resolved transient absorption spectroscopy. The fluorescence intensity ratio (R 450/525 ) of 9-acridinecarboxylic acid showed a 7-fold change in the physiological pH range (pH ∼ 5–8), and it can be served as an excellent ratiometric and lifetime pH indicator. Analysis of the picosecond and femtosecond-resolved spectra combined with theoretical calculation revealed that 9-acridinecarboxylic acid existed in zwitterionic form at acidic condition with emission centered at 480 nm and lifetime of about 2.1 ns and anionic form at alkaline condition with emission centered at 430 nm and lifetime of about 6.6 ns. As pH decreased, the protonation of heterocyclic N atom resulted in the redshift of fluorescence peak and shortening of the fluorescence lifetime. Moreover, 9-acridinecarboxylic acid exhibited good selectivity, photostability and excellent biocompatibility and was successfully applied for intracellular pH imaging by ratiometric fluorescence.
研究领域:
分析化学
·
生物物理学
·
光谱学
·
荧光成像
·
分子生物学
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产品关联
DOI
高影响因子
ANALYST
·
2018
·Vol. 143
·期 20
·5054-5060
·
IF 3.864
Bo Lin,Li Fan,Jinyin Ge,Wenjia Zhang,Caihong Zhang,Chuan Dong,Shaomin Shuang
A mitochondrial-targeted pH fluorescent probe 4-(2-(6-hydroxynaphthalen-2-yl)vinyl)-1-methylpyridin-1-ium was facilely synthesized via the carbon–carbon double bond bridging of 6-hydroxy-2-naphthaldehyde and 1,4-dimethylpyridinium iodide salt. The probe exhibited remarkable pH-dependent behavior in the linear range of 7.60–10.00, with a pKa value of 8.85 ± 0.04 near mitochondrial pH. A significantly large Stokes shift of 196 nm was obtained, which reduces the interference of excitation light. Application of the probe in live HepG2 cells indicated that the probe had excellent mitochondrial targeting ability and was successfully used to visualize mitochondrial pH fluctuations in live cells.
研究领域:
化学
·
生物化学
·
细胞生物学
·
荧光成像
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 15
·2097-2104
·
IF 2.378
Zhenzhen Feng,Yanyun Ma,Bingjie Li,Leiliang He,Qing Wang,Jin Huang,Jianbo Liu,Xiaohai Yang,Kemin Wang
Acatastatic pH values of organelles are associated with cell dysfunction and various diseases, which may increase the risk of diabetes, obesity, cancer, Alzheimer's disease and so on. Thus, in situ monitoring of the pH of organelles is extremely essential. Herein, we designed ratiometric fluorescent nanoprobes based on self-assembly for imaging of mitochondrial pH in living cells. The nanoprobes consisted of three parts: (1) a β-cyclodextrin polymer (β-CDP) acted as the backbone of the nanoprobes; (2) two lipophilic dyes acted as the pH sensitive unit and reference unit, i.e. adamantane-labeled fluorescein (Ad-F) and adamantane-labeled rhodamine (Ad-R); (3) adamantane-labeled triphenylphosphonium (Ad-TPP) acted as the mitochondrial targeting unit. Confocal microscopy images proved that the nanoprobes have been successfully applied to monitor intracellular pH in the range of pH 4.0–8.0. Moreover, as evidenced by co-localization imaging, the nanoprobes also showed good ability to target mitochondria (Pearson correlation coefficient was 0.88). Additionally, the nanoprobes showed low toxicity, excellent pH reversibility, and flexibility of construction, and have the potential to monitor pH of other organelles if different targeting units are conjugated. Our strategy is expected to provide new tools for pH monitoring in specific organelles and diagnosis of pH related diseases.
研究领域:
细胞生物学
·
生物医学工程
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纳米技术
·
荧光成像
·
疾病诊断
✓ 已验证
DOI
高影响因子
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