SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Haimei Yang,Qianghong Zhao,Xian Wang,Yu Wu,Yuchen Su,Weili Wei
The monitoring of biological hypochlorous acid (HClO) in living cells is vital in clarifying its physiological functions. Generally, HClO fluorescent probes that can be used for intracellular detection are small organic molecules that require complex synthesis steps. Herein, the as-prepared WSe 2 quantum dots (QDs) without any further modification was verified to be a HClO fluorescent probe due to the unique chemical property of selenium. Compared with molecular probes, WSe 2 QDs displayed improved performance in HClO detection, such as the low limit of detection (1.1 nM), high selectivity, fast response, good aqueous solubility, friendly operation, excellent photostability, and pH-independent response. Furthermore, the most outstanding advantage of as-prepared WSe 2 QDs was their ready availability with up to the gram-large scale. WSe 2 QDs were successfully used for the detection of exogenous and endogenous HClO in living cells. Meanwhile, this probe was applied to discriminate normal cells and cancerous cells on the basal of their different HClO levels. The sensing mechanism of WSe 2 QDs to HClO was further carefully demonstrated and proposed to be HClO induced the oxidation and degradation of WSe 2 QDs into non-fluorescent products. We anticipate that the as-prepared WSe 2 QDs should have broad applications in chemical analysis and revealing the physiological function of HClO in vivo .
研究领域:
化学
·
生物学
·
材料科学
·
分析化学
·
细胞生物学
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-8
·
IF 14.7
Wu Qianbao,Yang Na,Xiao Mengjun,Wang Wei,Cui Chunhua
Near-neutral HCO 3 – aqueous solution plays an essential role in respiratory, mineralization and catalysis, yet the interconversion between hydrated CO 2 , HCO 3 – and CO 3 2– and the associated proton transfer under such proton-deficient conditions remain uncovered. Here we reveal that cation enables HCO 3 – to self-dissociate into OH – and CO 2 through a pH-independent process, where CO 2 hydration and subsequent proton transfer in acid-base reactions lead to the overall exchange of oxygen isotopes between HCO 3 – and H 2 O tracked by oxygen isotope-labeled Raman spectroscopy. Isolating HCO 3 – from cations with crown ether impedes HCO 3 – dissociation and the following reactions. Further molecular dynamics simulations demonstrate that the interplay between HCO 3 – and hydrated cations drives HCO 3 – dissociation. This study suggests a natural proton channel upon coupling HCO 3 – with cations.
研究领域:
化学
·
生物化学
·
催化
·
光谱学
·
分子动力学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2025
·
IF 9.6
Jie Wang,Shenyuan Ouyang,Gaolong Lin,Dingwei Li,Bingyu Ding,Yumo Chen,Bingjie Tong,Ting Ouyang,Helin Xu,Wenzhi Wu
Current therapies for ulcerative colitis (UC) face critical challenges, including systemic toxicity and inadequate mucosal regeneration. Herein, we present a pioneering dual-mechanism therapeutic platform integrating pH-independent acid-treated sucralfate (ASF) with autophagy-inducing spermidine (Spd) to synergistically address UC pathogenesis. Unlike conventional pH-dependent barrier therapies, ASF forms a mechanically tunable, injectable hydrogel that adheres robustly to colonic mucosa regardless of local pH. Spermidine was easily mounted into ASF hydrogel matrix via electrostatic interaction, with more than 90 % encapsulation efficiency. Moreover, the mechanical strength of ASF hydrogel was precisely modulated by adjusting spermidine amount in formula, making it suitable for effective gut mucosa coverage. Importantly, in vitro permeability test showed that spermidine-loaded ASF hydrogel (Spd-ASF) demonstrated the selective barrier functionality, blocking > 80 % of Escherichia coli and LPS penetration while allowing nutrient permeability. Moreover, micro-CT images demonstrated that rectally infused Spd-ASF hydrogel was uniformly adhered to the colon wall at least for 8 h. In DSS-induced colitis mice, rectally administrating Spd-ASF hydrogel uniquely restored colon length to near-normal levels ( vs. 30 % shortening in controls), reduced proinflammatory cytokines (TNF-α, IL-6, IL-1β) by 60–75 %, and doubled goblet cell density. Mechanistically, Spd-ASF reprogrammed macrophage behavior by activating autophagy and enhancing efferocytosis, driving M2 polarization to resolve inflammation. Notably, Spd-ASF uniquely reversed dysbiosis, elevating beneficial Lactobacillus while suppressing colitis-associated Muribaculaceae and Clostridia . This study is the first to combine mucoadhesive biomaterial engineering with autophagy-mediated immunomodulation, offering a paradigm shift in UC therapy by simultaneously shielding the mucosa and reprogramming inflammatory pathways. Statement of significance Here, autophagy-inducing spermidine (Spd) and pH-independent acid-treated sucralfate (ASF) were combined to create the novel dual-mechanism hydrogel (Spd-ASF), which works in concert to combat UC pathogenesis. The selective barrier functioning of Spd-ASF hydrogel was established by its capacity to permit nutritional permeability while preventing the transfer of toxins (LPS) and pathogenic bacteria ( E. coli ). Additionally, rectally infused Spd-ASF hydrogel was consistently attached on the colon wall for at least eight hours, as seen by micro-CT images. Spd-ASF therapy doubled the density of goblet cells, decreased proinflammatory cytokines (TNF-α, IL-6, and IL-1β) by 60–75 %, boosted helpful Lactobacillus , and lowered pathogenic Muribaculaceae and Clostridia . It also restored colon length in DSS-induced colitis animals. Spd-ASF's therapeutic action was closely linked to promoting cellular efferocytosis and triggering macrophage autophagy.
研究领域:
生物医学工程
·
免疫学
·
胃肠病学
·
药物递送系统
·
生物材料
·
炎症性肠病（IBD）
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED CATALYSIS B-ENVIRONMENTAL
·
2016
·
IF 8.328
Hongying Zhao,Ying Chen,Qiusheng Peng,Qingning Wang,Guohua Zhao
A novel bifunctional MOF(2Fe/Co)/CA cathode with a high surface possessing area, and good electrocatalytic and photocatalytic activity, was proposed for the solar photo–electro–Fenton (SPEF) process. The optimal nFe: nCo ratio was 2:1 for successfully introducing MOF(2Fe/Co) on the surface of CA, which is due to the specific interaction affinity of FeOOH between MOF(2Fe/Co) and CA. Continuous on–site generation of H 2 O 2 through an oxygen reduction reaction (ORR) was achieved over the CA cathode. The electron transfer number (n) of the ORR with CA was 2.5 in the selected potential range. Moreover, the addition of MOF can behave as the active sites for enhancing the ORR activity and hence can increase the yield of the H 2 O 2 . Simultaneously, the photoinduced electron (e cb − ) of MOF(2Fe/Co) efficiently in–situ decomposed H 2 O 2 to form hydroxyl radicals ( OH). The observation indicated that the e cb − is an efficient and new Fenton–like catalysts that is pH–independent. Efficient photo–electro–catalytic performance was exhibited when applied to degrade Rhodamine B (RhB) and dimethyl phthalate (DMP). Almost 100% RhB removal and 85% DMP removal were reached in 45 min and 120 min, respectively. The MOF(Fe/Co)/CA presented very low iron and cobalt leaching (<0.5 ppm) even in an acidic media, and it retained an efficient degradation efficiency in the wide pH range of 3–9.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）