SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2019
·
IF 2.931
Yingying Zhang,Yanliang Zhao,Yingnan Wu,Bing Zhao,Liyan Wang,Bo Song
Lysosome as the typical acidic organelle involved in many biological processes, the dysfunction of its pH would alter many diseases. Small-molecule fluorescent probe has been considered as vital tool for monitoring pH fluctuation in living cells. Herein, a hemicyanine based ratiometric fluorescent probe was synthesized, namely, 2,3-trimethy-3-[2-(dimethyl-amino-4-yl) vinyl]-3 H -benzo[ e ]indole ( BiDL ), for rapidly detection pH under acidic conditions. BiDL exhibited ratio fluorescence emission ( F 534nm / F 622nm ) characteristic with p K a 4.25. BiDL showed good linearly response in pH 3.4–4.82, indicating that the probe can be used for quantitative detection pH. The probe also displayed large Stokes shift (112 nm/201 nm) under neutral and acidic conditions, respectively, which could effectively reduce the excitation interference. BiDL had excellent cell membrane permeability , good photo-stability and low toxicity in living cells. The dual-channel confocal fluorescent microscopic ratiometric imaging application of pH in HeLa cells and lysosome were achieved successfully, indicating that BiDL has good potential for investigating lysosome-relevant pathological and physiological processes.
研究领域:
生物化学
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医学
·
细胞生物学
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DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Jun Zhang,Chao Hou,He Yu,Md All Amin Newton,Binjie Xin,Tingxiao Li
To address persistent challenges in wound care, including exudate management, infection prevention, and healing monitoring, this study developed a multifunctional fiber dressing using electrospinning technology. The system integrates a patterned hydrophobic polylactic acid/zinc oxide (PLA/ZnO) inner layer providing antimicrobial protection and exudate drainage; a polyacrylonitrile/polydopamine (PAN/PDA) intermediate water transport layer enabling unidirectional exudate transfer; and an outer PAN-based functional membrane incorporating sodium polyacrylate (SPA), phenol red (PSP), and tetracycline hydrochloride (TCH) for controlled drug release and visual wound status monitoring. The comprehensive evaluation revealed exceptional performance: rapid exudate removal within 3.35 s, 96 h sustained antibacterial drug release (68.6% cumulative release), pH-responsive chromatic transition (yellow-to-red at pH 5–9), and significant antimicrobial efficacy against Escherichia coli and Staphylococcus aureus (inhibition zone diameters of 10 and 18 mm, respectively). The dressing exhibited a balance of mechanical strength (6.1 MPa tensile strength) and breathability (13.4 mm/s air permeability rate). By integrating three core functions─efficient exudate management, antimicrobial regulation, and intelligent healing monitoring─this multifunctional architecture demonstrates clinical applicability in postoperative care and chronic wound therapy.
研究领域:
生物材料学
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伤口护理
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药剂学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lu Shi,Feifei Cao,Limin Zhang,Yang Tian
The development of switches responding to specific pH changes was particularly useful in wide application fields. Owing to flexible switches simulated by pH, i-motif DNAs are widely used as a pH sensor. But its character of structure transition strongly dependent on acidic pH severely hampers the application of i-motif DNA in physiological media. Herein, we report the stable i-motif structure formed at a physiological pH triggered by spatial confinement of silica nanochannels. Three classic DNA chains containing 21-mer i-motif domain base-pairs and a single-stranded multiply (T)n spacer, 5′-COOH-(T)n-CCCTAACCCTAACCCTAACCC-3′, were employed to evaluate the enhanced stability of i-motif structure. Compared to their free states in a dilute solution, the transition pH of all i-motif DNAs decorated in nanochannels remarkably shifts toward a neutral pH. Moreover, the transition midpoint can be tuned sensitively over the physiologically relevant pH range through slightly varying the length of T base spacer. Density functional theory (DFT) calculations validate that the increased proton density in a nanochannel triggers the formation of an i-motif structure under a neutral pH. Finally, this i-motif DNA based nanochannels electrode was successfully employed to monitor pH in brain microdialysates followed by cerebral ischemia. The present approach is not limited by fundamental investigation for DNA conformation but may extend toward the manipulation of i-motif based structures for artificial molecular machines and signaling systems.
研究领域:
生物化学
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分子生物学
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医学检测
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产品关联
DOI
PubMed
高影响因子
TALANTA
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2016
·
IF 4.035
Mingtai Sun,Libo Du,Huan Yu,Kui Zhang,Yang Liu,Suhua Wang
It is crucial to monitor intracellular pH values and their fluctuation since the organelles of cells have different pH distribution. Herein we construct a new small molecule fluorescent probe HBT-O for monitoring the subtle pH values within the scope of neutral to acid in living cells. The probe exhibited good water solubility, a marked turquoise to olivine emission color change in response to pH, and tremendous fluorescence hypochromatic shift of ∼50 nm (1718 cm −1 ) as well as the increased fluorescence intensity when the pH value changed from neutral to acid. Thus, the probe HBT-O can distinguish the subtle changes in the range of normal pH values from neutral to acid with significant fluorescence changes. These properties can be attributed to the intramolecular charge transfer (ICT) process of the probe upon protonation in buffer solutions at varied pH values. Moreover, the probe was reversible and nearly non-toxic for living cells. Then the probe was successfully used to detect pH fluctuation in living cells by exhibiting different fluorescence colors and intensity. These findings demonstrate that the probe will find useful applications in biology and biomedical research.
研究领域:
生物化学
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细胞生物学
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医学研究
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Xu Tang,Zhi Zhu,Yun Wang,Juan Han,Liang Ni,Lei Wang,Huiqin Zhang,Jing Li,Yue Qiu
A novel dual site controlled probe has been developed on the basis of rhodamine and biphenylcarbonitrile unit. The probe showed not only rapid fluorescence response to pH, but also good colorimetric recognition performance for copper ions in the HEPES buffer solution (pH 7.0). The optimal geometric configuration of the probe RhB-H with different structures and the [RhB-H-Cu 2+ ] complex were calculated at B3LYP level by DFT method. On this basis, the response mechanism of pH was analysed combining with the 1 H NMR titration experiment. Moreover, the cytotoxicity studies indicated that the probe had good biocompatibility and low toxicity. Then the probe was also successfully applied on real-time monitoring of the intracellular pH changes by exhibiting different fluorescence and emission intensity. These results demonstrated that the probe could be usefully involved in biological and biomedical research.
研究领域:
生物化学
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分子生物学
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分析化学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingying Chen,Danni Wu,Xiaoye Deng,Yingjie Li,Xiaoqin Sun,Junjie Ma,Congjin Chen,Xiufen Liao,Xijun Liu
Carbon dots (CDs), revolutionary carbon-based nanomaterial, have demonstrated tremendous potential for applications across multiple areas. Chitin-rich shrimp shells are a sustainable and renewable resource, and their utilization can turn waste into treasure. This work is dedicated to efficiently preparing nitrogen-doped green fluorescent CDs (N-CDs) for multi-modal applications from waste shrimp shells by hydrothermal methods. The detailed spectroscopic characterization reveals that the N-CDs possess excellent optical properties. The quantum yield is as high as 33.2%. Specifically, the N-CDs show excellent selectivity and sensitivity in the quantitative detection of Fe 3+ . Additionally, in response to pH changes, the surface functional groups of the N-CDs undergo protonation and deprotonation, leading to corresponding alterations in fluorescence colors. Capitalizing on this characteristic, a visual portable pH-measuring platform is developed. This platform ingeniously integrates nanoscale optical sensors with smartphone image processing technology, enabling real-time and highly accurate monitoring of environmental pH levels. Furthermore, they are ideal materials for fluorescence anti-counterfeiting detection due to their unique optical properties. This work simultaneously accomplishes the reuse of waste biomass and provides novel insights for multi-orbital applications, including metal ion detection, pH monitoring, and the anti-counterfeiting field.
研究领域:
材料科学
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环境科学
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光学工程
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生物传感
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Junyong Sun,Pinghua Ling,Feng Gao
The accurate and sensitive monitoring and imaging of mitochondrial pH in living cells play vital roles in chemical biology and biomedicine. Herein, we design a novel ratiometric fluorescent chemical probe for monitoring and imaging the pH of mitochondria in living cells based on congo-red (CR)-modified dual-emission semiconducting polymer dots (Pdots) via a competitive fluorescence resonance energy transfer (FRET) mechanism. The Pdots are synthesized by triphenylphosphonium (TPP)-modified polyoxyethylene nonylphenylether (CO-520), poly(9,9-dioctylfluorenyl-2,7-diyl) (PFO), poly(9,9-dioctylfluorene)-co-(4,7-di-2-thienyl-2,1,3-benzothiadiazole) (PF-DBT5), and poly(styrene-co-maleic anhydride) (PSMA) via a nanoprecipitation method, and the prepared Pdots are further chemically linked with pH-sensitive, nonfluorescent CR to obtain the mitochondria-targeted pH fluorescent probes. This Pdots-based probe consists of two luminescent components including PFO and PF-DBT5 as fluorescence donors, an acid–base indicator CR as an energy acceptor, and TPP as the mitochondria-targeting group. At a different pH region, the FRET efficiency between CR and PFO or CR and PF-DBT5 can be modulated. This probe exhibits good biocompatibility, a wide pH detection range from 2.57 to 8.96, good reversibility, high selectivity, and excellent photostability for pH monitoring. This probe allows for the detecting and imaging of mitochondrial pH in living cells with satisfactory results.
研究领域:
化学生物学
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生物医学
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产品关联
DOI
PubMed
高影响因子
Materials Today Chemistry
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2022
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IF 7.613
X. Liu,J. Song,B. Yin,T. Jiang,H. Pang,L. Yang,S. Wu
Ascorbic acid (AA) is a critical nutrition and chemical for humans, due to its crucial role in metabolic processes and medical treatments. It is necessary to develop a simple and rapid method for real-time tracking AA level in the body. In this work, we fabricated a powerless, gas-permeable, flexible and transparent AA biosensor based on carbon micromesh electrode (CME). The AA biosensor worked without an external power supply and exhibited excellent performances, including high transmittance (>95%), wide detection range (1 μM–1 mM), low limit of detection (10 nM), good selectivity and long-term storage stability (30 days). In addition, we integrated a pH-sensing electrode to correct the AA analysis. A layer of polyaniline (PANI) film was electrodeposited on the carbon micromesh as pH-sensing electrode that had an average sensitivity of 58.1 mV/pH with a high correlation coefficient ( R 2 = 0.997) within the physiological range of human body fluids (pH 4.0–8.7). To verify the availability of the CME in practical application, the Ag/C electrode was used as reference electrode to fabricate the integrated dual-functional biosensors for artificial tears detection. The CME-based biosensors showed good sensitivity, selectivity and correlation coefficient, indicating its excellent practicability towards wearable electronics.
研究领域:
生物传感技术
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医学检测
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材料科学
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产品关联
DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 9
·1925
·
IF 4.2
Wuliang Sun,Junhui Dong,Wenbo Li,Xiaobo Gao,Jun Liu,Ding Nan
In recent years, renewable and sustainable triboelectric nanogenerators have attracted attention due to their high energy conversion rate, and enhancing their functionality further contributes to their applicability across various fields. A pH-sensitive triboelectric nanogenerator (pH-TENG) has been prepared by electrostatic spinning technology, with anthocyanin as the pH indicator and environmentally friendly polyvinyl alcohol (PVA) as the substrate. Among many friction-negative materials, the pH-TENG exhibits the best combination with fluorinated ethylene propylene (FEP) and yields an open-circuit voltage of 62 V, a short-circuit current of 370 nA, and a transferred charge of 21.8 nC. At a frequency of 3 Hz, it can charge a 4.7 μF capacitor to 2 V within 45 s, effectively powering a thermometer. Furthermore, the presence of anthocyanin does not affect the pH-TENG’s power generation performance and enables the monitoring of a wide range of environmental pH changes, with an ΔE change of 28.8 ± 7.6. Therefore, pH-TENG prepared with environmentally friendly materials can bring new available materials to the biological and medical fields.
研究领域:
生物医学工程
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材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2020
·
IF 10.652
Xing Dong,Junjie Chi,Changmin Shao,Lanjie Lei,Lei Yang,Chao Zhao,Hong Liu
Tumor microenvironment (TME) is a pivotal factor in tumor progression. Here we report an injectable multifunctional hydrogel microsphere with responsive photonic structure for in vivo monitoring of TME pH. The pH determination is based on measurement of the characteristic reflection peak of the hydrogel microsphere in near-infrared (NIR) region that is sensitive to pH, and decreasing pH leads to a blue shift of the peak. With deep tissue penetration ability of the NIR light, the pH of normal and tumor tissues of mice are continuously monitored in vivo using the microspheres injected subcutaneously. The biocompatibility and the cytotoxicity of the material is investigated based on MTT assays to evaluate its potential for long-term continuous in vivo monitoring of TME pH. A drug for cancer treatment is also preloaded into the hydrogel microsphere for simultaneous drug release in TME and monitoring of the treatment effectiveness of drug by the TME pH tests.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Materials Chemistry B
·
2019
·Vol. 7
·期 16
·2626-2633
·
IF 5.047
Nan Pan,Juanrong Qin,Pingping Feng,Zeke Li,Botao Song
Real-time monitoring of wound pH may provide information about the wound healing status and potential bacterial infection. Herein, we integrated the biocompatible color changing substance curcumin into a fibrous material, capable of in situ real-time visually monitoring the wound pH. The results indicate that the curcumin-loaded fibrous mat exhibits an obvious pH-dependent color change from yellow to red brown with a change in pH from 6.0 to 9.0, which can be easily detected by the human naked eye. Moreover, the wound pH conditions can be determined with the aid of a smart phone App after image analysis. Due to their flexibility, the fibrous materials have been further processed into various shapes from 1D to 3D for fitting the irregular wounds. It is believed that smart fibrous materials that can simultaneously real-time monitor the wound pH and repair the wound may change wound management to a convenient and comfortable way.
研究领域:
生物医学工程
·
材料科学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jinglei Yang,Bohan Zhang,Qingling Nie,Ruping Zheng,Ping Lin,Chuyi Wang,Yudong Lu,Yunli Xu,Ruiyun You
Uric acid, urea, and other metabolites in urine after exercise often reflect chronic injury syndrome in athletes. However, traditional urine detection methods have issues such as high costs and low detection sensitivity. SERS can rapidly, continuously, and sensitively monitor metabolites in human urine. In this research, a combined SERS substrate (CMBCM@Ag NPs@PGA) was developed based on the carboxymethyl modification of the bacterial cellulose membrane (BCM) surface. The numerous carboxyl groups on the CMBCM surface made it easier for silver ions to be adsorbed, leading to their conversion into silver nanoparticles (Ag NPs) when a reducing agent was introduced. This process allowed the nanoparticles to firmly adhere to the CMBCM surface, forming a uniform and stable “hot spot.” “The CMBCM@Ag NPs@PGA substrate maintains excellent stability and sensitivity in the assay.” It can detect very small amounts of urea and uric acid in urine with high sensitivity, with LOD of 1.05 μM for urea and 0.0075 μM for uric acid. Additionally, it exhibits good stability, antibacterial properties, and cell compatibility. In addition, the substrate can be used as a sensor to monitor pH in real-time. This expands the use of cellulose in flexible SERS sensing and detecting human exercise metabolic health.
研究领域:
生物医学工程
·
分析化学
·
运动医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）