FEBS Open Bio
·
2020
·Vol. 10
·期 12
·2733-2739
·
IF 2.231
Sai-sai Ding,Jin-peng Zhu,Yang Wang,Bin Wu,Zongpei Zhao
In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco-friendly process called biobleaching, which can help minimize the usage of chlorine in the delignification process. However, a bottleneck in the adoption of biobleaching is the cost of xylanase and the requirement that xylanase be active and stable at extreme conditions. Here, we investigated whether using sodium alginate beads to immobilize an extracellular xylanase from Bacillus subtilis (Lucky9) can reduce the potential cost of enzyme usage. The optimal pH and the activity of the immobilized enzyme were increased at optimal temperature compared with the free enzyme. In addition, immobilized xylanase was shown to be more stable than free xylanase. The results of this study suggest that the immobilized xylanase has potential applications in the biobleaching industry.
研究领域:
生物技术
·
酶工程
·
制浆造纸工业
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PubMed
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Wen-Han Yu,Zhi-Qiang Gan,Jia-Rong Wang,Yu Zhao,Jun Han,Li-Feng Fang,Xiu-Zhen Wei,Ze-Lin Qiu,Bao-Ku Zhu
Nanofiltration (NF) membrane has an extensive application in the field of water treatment, but weakly negative surface-charge and even charge inversion in acidic environment restrict wide applications of traditional aromatic polyamide (PA) thin film composite (TFC) NF membrane in a broad pH range. In this work, 4, 4′-diaminostilbene-2, 2′-disulfonic acid (DSDA), a novel acidic aqueous monomer , was introduced into interfacial polymerization to fabricate a TFC NF membrane with enhanced negative charge and pH stability. The effects of interfacial polymerization parameters were investigated to improve membrane performance. The optimal membrane exhibits a pure water permeance (PWP) of 10.0 ± 0.5 L m −2 h −1 ·bar −1 and a high Na 2 SO 4 rejection (i.e., ~97.0%). The resultant membrane remains a strongly negative charge when the pH value is changed from 2 to 12. Due to enhanced negative charge, Donnan exclusion plays a significant role in membrane separation process, which results in a huge superiority in removal of Na 2 SO 4 , Na 3 PO 4 and K 2 Cr 2 O 7 in a broad pH range especially in acidic environment. Hence, it is demonstrated that the negative-charged NF membrane prepared in this work has improved and stable separation performance under a broad pH range, which expands application scopes of NF membrane, especially in removing Cr (VI) and phosphate in acidic environments. Besides, this study also provides a feasible route for preparation of suitable TFC NF membrane for working in acidic environment via introducing strongly acidic groups in selective layer.
研究领域:
环境科学
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材料科学
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DOI
高影响因子
Journal of Analytical Science and Technology
·
2021
·Vol. 12
·期 1
·1-12
·
IF 2.769
Chen Kangni,Li Yanbo,Shang Jinyan,Li Heping
Biothiols play important roles in various physiological and biological processes, which closely related to many diseases. Hydrazine is widely used in the chemical industry, but it is harmful to humans and animals. Therefore, it is very important to develop a fluorescent probe that can simultaneously detect biological thiols and hydrazine. In this work, a new fluorescent probe (2E,4Z)-2-(benzo[d]thiazol-2-yl)-5-chloro-5-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)penta-2,4-dienenitrile (BCD) was synthesized by integrating coumarin and benzothiazole acetonitrile. Featured with four binding sites and different bonding mechanism between probe with biothiols and hydrazine, this probe exhibited fluorescent turn-on for distinguishing Cys, Hcy, GSH and hydrazine with 760-, 8-, 6- and 637-fold fluorescent intensity increase at 502, 479, 476 and 458 nm, respectively, through different excitation wavelengths. Research on the effect of pH on the fluorescent performance of BCD shows that the probe exhibits superior stability in a weakly alkaline to weakly acidic environment, which will facilitate the detection of biological thiols or hydrazine hydrate by BCD. Selectivity studies have shown that the probe has high specificity to biological thiols and hydrazine, which is of great significance to the application of BCD.
研究领域:
分析化学
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生物化学
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DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2019
·
IF 0
Sefiu A. Rasaki,Tiju Thomas,Minghui Yang
Due to high surface area and large pore volume, nanostructure zinc oxides/its composites have gained a lot of research interests for heavy metal removal from water. Low stability of the ZnO at low and high pH is however a problem. In this work, ZnO@MgO, ZnO and MgO are synthesized via co-precipitation, followed by calcinations. The samples show remarkable removal of Pb(II) from water when compared to commercial samples; the best results is obtained using ZnO@MgO which exhibit adsorption capacity of 250 mg g −1 equivalent to 100% removal capacity. The optimum removal capacity is retained in a wide range of pH (i.e. pH 6–9) at temperature 30 °C. Low adsorption capacity coupled with low stability in acidic condition; a problem associated with ZnO-based adsorbents when it is being used for the removal of heavy metals from water/wastewater; often prevents this system from being practically used; is overcome here. The ZnO@MgO adsorbent shows chemical stability over wide range of pH(2–9). The adsorption is chemisorptive in nature, endothermic and evidently aided by high surface heterogeneity (△H° ˜25 kJ mol-1 and △S°˜110.6 J mol −1 K −1 ). Appreciable adsorption capacity over wide range of pH-value, high recovery and reusability results from nanosphere morphology of the composite sample; all these novel properties are attributed to the strong electronic coupling effect between MgO and ZnO. This work is expected to spur further research on ZnO-based composite materials in allied areas, including (i) mitigation of atmospheric pollution and (ii) water treatment processes.
研究领域:
环境科学
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材料科学
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DOI
BIOSENSORS & BIOELECTRONICS
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2025
·
IF 10.5
Jiuhong Zhao,Jinnan Zhao,Xiaoyu Zhang,Guixia Ling,Peng Zhang
The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the in vivo DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.
研究领域:
生物化学
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医学检测
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纳米材料科学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2024
·
IF 3.7
Yuhang Wei,Qingpeng Zhu,Weiwei Xie,Xinyue Wang,Song Li,Zhiming Chen
Efficient and convenient treatment of industrial dyeing wastewater is of a great significance to guarantee human and animal health. This work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on amino-functionalized ZnFe 2 O 4 nanoparticles (ZnFe 2 O 4 -laccase) and its application for degradation of textile dyes. Due to the existence of a large number of oxygen vacancies on the surface of the ZnFe 2 O 4 nanoparticles, negative ions accumulated on the magnetic carriers, which resulted in a harsh optimal pH value of the ZnFe 2 O 4 -laccase. Laccase activity assays revealed that the ZnFe 2 O 4 -laccase possessed superior pH and thermal stabilities, excellent reusability, and noticeable organic solvent tolerance. Meanwhile, the ZnFe 2 O 4 -laccase presented efficient and sustainable degradation of high concentrations of textile dyes. The initial decoloration efficiencies of malachite green (MG), brilliant green (BG), azophloxine, crystal violet (CV), reactive blue 19 (RB19) and procion red MX-5B were approximately 99.1%, 95.0%, 93.3%, 87.4%, 86.1% and 85.3%, respectively. After 10 consecutive reuses, the degradation rates of the textile dyes still maintained about 98.2%, 92.5%, 83.2%, 81.5%, 79.8% and 65.9%, respectively. The excellent dye degradation properties indicate that the ZnFe 2 O 4 -laccase has a technical application in high concentrations of dyestuffs treatment.
研究领域:
环境科学
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生物技术
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DOI
高影响因子
Marine Drugs
·
2024
·Vol. 22
·期 6
·287
·
IF 4.9
Han-Zhong Guo,Dou Wang,Hui-Ting Yang,Yu-Le Wu,Yong-Cheng Li,Guang-Hua Xia,Xue-Ying Zhang
To promote the bioconversion of marine chitin waste into value-added products, we expressed a novel pH-stableMicromonospora aurantiaca-derived chitinase,MaChi1, inEscherichia coliand subsequently purified, characterized, and evaluated it for its chitin-converting capacity. Our results indicated thatMaChi1 is of the glycoside hydrolase (GH) family 18 with a molecular weight of approximately 57 kDa, consisting of a GH18 catalytic domain and a cellulose-binding domain. We recorded its optimal activity at pH 5.0 and 55 °C. It exhibited excellent stability in a wide pH range of 3.0–10.0. Mg2+(5 mM), and dithiothreitol (10 mM) significantly promotedMaChi1 activity.MaChi1 exhibited broad substrate specificity and hydrolyzed chitin, chitosan, cellulose, soluble starch, andN-acetyl chitooligosaccharides with polymerization degrees ranging from three to six. Moreover,MaChi1 exhibited an endo-type cleavage pattern, and it could efficiently convert colloidal chitin intoN-acetyl-D-glucosamine (GlcNAc) and (GlcNAc)2with yields of 227.2 and 505.9 mg/g chitin, respectively. Its high chitin-degrading capacity and exceptional pH tolerance makes it a promising tool with potential applications in chitin waste treatment and bioactive oligosaccharide production.
研究领域:
酶学
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微生物学
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生物化学
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PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2017
·Vol. 27
·期 5
·1721-1727
·
IF 1.461
Wang Fanwei,Duan Hongdong,Xing Dianxiang,Yang Geng
One novel turn-on fluorescence probe founded on conjugated pyrazole Schiff base for detecting Al 3+ was invented. The UV–vis and fluorescence spectrometer were employed to explore optical properties of this probe. The results got from those experiments indicated that this fluorescence probe manifested excellent sensitivity and selectivity for Al 3+ compared with other cations examined(Ag + , Co 2+ , Mg 2+ , Cu 2+ , Ni 2+ , Pb 2+ ,and Zn 2+ ). In addition, this probe displayed a more rapid response and remained stable between pH 6 and 9 by investigating the fluorescence intensity under different response time and various pH values. Remarkably, the detection limit for Al 3+ could lower to 1.0×10 −9 M. Therefore, the probe could be potentially applied to the environment for the detection of Al 3+ , and the availability in biological range of pH that could be further studied to make this probe apply to biological systems in the future.
研究领域:
分析化学
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环境科学
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生物医学
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DOI
PubMed
高影响因子
DYES AND PIGMENTS
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2025
·
IF 4.2
Xiangning Fang,Guanyu Jiang,Qinglong Qiao,Xiaogang Liu,Zhaochao Xu
Rhodamines are widely utilized for fluorescence switching, biosensing, and super-resolution imaging due to their dynamic equilibrium between a non-fluorescent "closed" spiro-ring form and a fluorescent "open" xanthylium form. This equilibrium has inspired extensive efforts to chemically modulate their spiro-ring behavior. In this study, we uncover a novel mechanism in aniline-rhodamine ( PR ), where the interplay of NH protonation, deprotonation, and hydrogen bonding collaboratively governs spirolactone ring opening. Notably, this interplay stabilizes the ring equilibrium in aqueous solutions within the pH range of 4–10, minimizing sensitivity to pH fluctuations. Leveraging this mechanism, we designed an aniline-rhodamine derivative, EPR , which maintains stable fluorescence regardless of lysosomal pH variations. This stability enables consistent, high-resolution imaging of lysosomal dynamics under both physiological and pathological conditions, offering deep insights into lysosomal functions within cellular regulatory networks. The NH groups in PR establish a new regulatory axis for rhodamine ring switching, providing a promising foundation for developing next-generation fluorescent probes with enhanced stability and functionality.
研究领域:
细胞生物学
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分子成像
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化学生物学
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生物医学工程
·
超分辨显微技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yanan Zhang,Yimin Wang,Ningjing Huang,Wei zheng,Hang Guo,Chunyin Li
A sensor for non-enzymatic hydrogen peroxide (H2O2) was developed based on silicon quantum dots (SiQDs). The fluorescent SiQDs were synthesized using a one-pot hydrothermal method. The SiQDs were made water-soluble and pH-stable by covering their surfaces with amino and hydroxyl groups. Additionally, the prepared SiQDs had a large Stokes shift (131 nm), excellent photostability (442 min), and hypotoxicity (e.g., 99% of cell viability was kept after a 24 h-treatment at 8.0 mg/mL of the SiQDs). The dynamic quenching effect induced by H2O2 effectively suppresses the blue fluorescence of SiQDs. Under the optimum conditions, the SiQD-based H2O2 sensor exhibited a wide linear range (10–1000 μM), high sensitivity and selectivity, and a good anti-interference ability. The detection limit and relative standard deviation were 1.7 μM and 4.5% (c = 20 μM, n = 7), respectively. Accordingly, a simple, enzyme-free, and highly efficient method was established for detecting H2O2 in aqueous solutions. Moreover, this eco-friendly fluorescent sensor successfully determined H2O2 in human serum samples with 92%–102% recoveries.
研究领域:
生物化学
·
分析化学
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DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Su Yafei,Peng Huawen,Liu Xufei,Li Jiapeng,Zhao Qiang
Cation separation under extreme pH is crucial for lithium recovery from spent batteries, but conventional polyamide membranes suffer from pH-induced hydrolysis. Preparation of high performance nanofiltration membranes with excellent pH-resistance remains a challenge. Here we synthesize a high performance nanofiltration membrane (1,4,7,10-Tetraazacyclododecane (TAD)−1,3,5-Tris(bromomethyl)benzene (TBMB) thin film composite membranes (TFCMs)) with excellent pH-stability through interfacial quaternization reaction between TAD and TBMB. Due to the high stability of “C-N” bonds in TAD-TBMB TFCMs, its separation performance is stable even after 70 days immersion in concentrated acid (3 M H 2 SO 4 , HNO 3 , or HCl) and base (3 M NaOH), which is at least 15 times more stable than benchmark commercial membranes. The membrane shows an overall separation performance (11.3 L m −2 h −1 bar −1 (LMHB), R Co2+ : 97% in 2 M H 2 SO 4 ) due to the size sieving and the intensified charge repulsion, outperforming many of the state-of-the-art membranes. Finally, the TAD-TBMB TFCM remains stable during 30-days continuous nanofiltration of 2 M H 2 SO 4 and leachate (2 M H 2 SO 4 , ions: 6.2 g L −1 ) from spent batteries.
研究领域:
材料科学
·
环境工程
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DOI
PubMed
高影响因子
CHEMISTRY-A EUROPEAN JOURNAL
·
2015
·Vol. 21
·期 9
·3791-3797
·
IF 5.731
Hanjun Sun,Haiwei Ji,Enguo Ju,Yijia Guan,Jinsong Ren,Xiaogang Qu
Graphical Caught on camera : By combining a microwave-assisted technique with hydrothermal treatment and using fluorinated graphene oxide (FGO) as a raw material, fluorinated graphene quantum dots (GQDs) and nonfluorinated GQDs are synthesized (see figure). Fluorinated GQDs show improved pH and photostability compared with nonfluorinated GQDs due to fluorine. Fluorinated GQDs are suitable for long-term cellular imaging. Herein, a new strategy has been developed through combining a microwave-assisted technique with hydrothermal treatment to reduce graphene waste and improve production yield of graphene quantum dots (GQDs) prepared by top-down methods. By using fluorinated graphene oxide (FGO) as a raw material, fluorinated GQDs and nonfluorinated GQDs can be synthesized. Additionally, in the fluorinated GQDs, the protective shell supplied by fluorine improves the pH stability of photoluminescence and the strong electron-withdrawing group, F, reduces the π- electron density of the aromatic structure; thus inhibiting reactivity toward singlet oxygen produced during irradiation and improving the photostability. Therefore, the as-prepared fluorinated GQDs with excellent photo- and pH stability are suitable for long-term cellular imaging.
研究领域:
材料科学
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生物医学工程
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）