ANALYTICA CHIMICA ACTA
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2023
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IF 6.2
Xuegang Li,Pengqi Gong,Xue Zhou,Shankun Wang,Yingxuan Liu,Yanan Zhang,Linh V. Nguyen,Stephen C. Warren-Smith,Yong Zhao
An advanced multi-parameter optical fiber sensing technology for EGFR gene detection based on DNA hybridization technology is demonstrated in this paper. For traditional DNA hybridization detection methods, temperature and pH compensation can not be realized or need multiple sensor probes. However, the multi-parameter detection technology we proposed can simultaneously detect complementary DNA, temperature and pH based on a single optical fiber probe. In this scheme, three optical signals including dual surface plasmon resonance signal (SPR) and Mach-Zehnder interference signal (MZI) are excited by binding the probe DNA sequence and pH-sensitive material with the optical fiber sensor. The paper proposes the first research to achieve simultaneous excitation of dual SPR signal and Mach-Zehnder interference signal in a single fiber and used for three-parameter detection. Three optical signals have different sensitivities to the three variables. From a mathematical point of view, the unique solutions of exon-20 concentration, temperature and pH can be obtained by analyzing the three optical signals. The experimental results show that the exon-20 sensitivity of the sensor can reach 0.07 nm nM −1 , and the limit of detection is 3.27 nM. The designed sensor gives a fast response, high sensitivity, and low detection limit, which is important for the field of DNA hybridization research and for solving the problems of biosensor susceptibility to temperature and pH.
研究领域:
生物医学工程
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分子生物学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Xuegang Li,Pengqi Gong,Qiming Zhao,Xue Zhou,Yanan Zhang,Yong Zhao
A novel DNA hybridization optical fiber sensor with temperature and pH compensation for detecting a common lung cancer gene of epidermal growth factor receptor (EGFR) gene was demonstrated in this paper. The paper proposes the first research to achieve dual surface plasmon resonance (SPR) in an Fiber Bragg grating (FBG) and implement a three-parameter detection of DNA sequence , temperature and pH using a single fiber. Probe DNA sequences (pDNA) and PAA/CS, a pH-sensitive material, are immobilized on the sensor surface for EGFR gene exon-20 detection and temperature and pH monitoring simultaneously. The effectiveness of this functionalized method has been proved by using an atomic force microscope (AFM) and scanning electron microscope (SEM). The limit of detection (LOD) of EGFR gene was experimentally verified to 13.5 nM. The advantages of fast response, high sensitivity, label free, plug in, low LOD and three-parameter detection demonstrated by this sensor in the experiment are important for the field of DNA hybridization research and solving the problems of biosensor susceptibility to temperature and pH.
研究领域:
生物医学工程
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分子生物学
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光学传感
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DOI
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 9
·5055-5064
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IF 3.361
Weikang Xu,Ruifang Zhao,Tingting Wu,Guixiang Li,Kun Wei,Liyan Wang
Sintered microsphere-based scaffolds provide a porous structure and high-resolution spatial organization control, show great potential for bone regeneration, mainly from biodegradable biomaterials including poly(lactic-glycolic acid) (PLGA). However, acidic monomer regeneration, mainly from biodegradable biomaterials including poly(lactic-glycolic acid) (PLGA). However, acidic monomers generated by PLGA degradation tend to cause tissue inflammation, which is the central issue of PLGA-based bone regeneration scaffolds development. In this work, calcium carbonate (CC)/hexagonal mesoporous silica (HMS)/PLGA sintered microsphere-based scaffolds were developed. The scaffolds possessed a three-dimensional (3D) network structure and 30–40% porosity. The degradation results indicated that CC/HMS/PLGA scaffolds could compensate for pH increased caused by PLGA acidic byproducts effectively. Degradation results showed that CC/HMS/PLGA scaffold could effectively compensate for the pH increase caused by PLGA acidic by-products. Composite CC additives can induce the increase of adhesive proteins in the environment, which is conducive to the adhesion of cells to scaffolds. Mesenchymal stem cells (MSCs) proliferation and osteogenic differentiation were evaluated by CCK-8 assay, alkaline phosphatase (ALP) activity, ALP staining, and Alizarin Red staining. The results showed that compared with HMS/PLGA scaffolds, the proliferation of MSCs cultured with CC/HMS/PLGA scaffolds was enhanced. When cultured on the CC/HMS/PLGA scaffolds, MSCs also showed significantly enhanced ALP activity and higher calcium secretion compared with the HMS/PLGA scaffolds. CC/HMS/PLGA sintered microsphere-based scaffolds provides an attractive strategy for bone repair and regeneration with better performance.
研究领域:
生物材料学
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组织工程学
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医学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
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IF 19
Zishuo Fang,Xiuxiu Liu,Tao Liu,Jing Zhang,Xinxin Liu,Mengjiao Zhu,Danfeng Jiang,Zhenyu Chu,Wanqin Jin
Wearable sweat sensors have emerged as a promising candidate for personalized health assessment due to their robust capabilities in non-invasive and dynamic monitoring of bioindicators. However, the ultralow concentration of metabolites and pH-induced signal fluctuations in sweat seriously affect the reliability of dynamic monitoring. To address these challenges, this study reports a non-invasive and sensitive wearable sensor that incorporates real-time pH calibration to accurately assess hyperuricemia via monitoring sweat uric acid (UA). To precisely capture the response of trace sweat UA, the surface roughness of hollow Mn 2 O 3 /Fe 2 O 3 is dramatically enhanced to improve interface contact and boost the charge transfer kinetics. Meanwhile, to eliminate the fluctuating pH influence, an integrated pH sensor continuously tracks sweat pH to calibrate the UA response, establishing the pH-dependent sensitivity calibration model. A rapid collection microchannel is also designed to efficiently transport fresh sweat while minimizing evaporation and contamination, further elevating the detection accuracy. During the 12-hour purine-controlled diet, the wearable sensor dynamically and accurately tracks the sweat UA, demonstrating a significant correlation with serum UA. This result confirms the feasibility of the wearable sensor for gout monitoring. This work establishes a non-invasive platform for the precise assessment of hyperuricemia, facilitating at-home early warning for gout.
研究领域:
可穿戴电子
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生物医学传感
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即时检测
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个性化医疗
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电化学传感器
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）