JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
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IF 9.7
Chuanwei Shi,Peng Liu,Yeping Liu,Zhexu Gai,Fei Yang,Yanzhao Yang
The microenvironment around the wounds of chronic disease patients often exhibits weakly alkaline conditions, primarily due to defects in the patients' blood glucose regulation, tissue hypoxia, accumulation of metabolic products, and bacterial infection and growth. To regulate the wound microenvironment across a broad pH range, a Fe-doped ZIF-8 nanozyme loaded with glucose oxidase (Fe-ZIF-8@GOx) was synthesized via a one-step in situ growth method. This nanozyme, with ZIF-8 serving both as a protective matrix and immobilization scaffold, enhances the enzyme's operational microenvironment and maintains functionality across a wide pH range. It exhibits the activities of cascade glucose oxidase (GOx), catalase-like (CAT-like), and peroxidase-like (POD-like) enzymes. When Fe-ZIF-8@GOx nanozymes encounter high glucose levels at the site of weakly alkaline chronic ulcers, the GOx particles catalyze the reduction of local pH, simultaneously triggering CAT-like enzyme activity to release O₂ and improve the wound microenvironment. The subsequent pH reduction induces the controlled release of iron ions, enabling Fe 2+ to react with endogenously produced hydrogen peroxide to generate reactive oxygen species (ROS), conferring a distinctive guest–carrier synergistic antibacterial effect. Both in vitro and in vivo studies confirm that this material effectively reduces inflammation, modulates macrophage polarization, enhances collagen deposition, and promotes angiogenesis, ultimately accelerating wound healing under chronic diabetic conditions. Thus, our work not only overcomes microenvironmental limitations in antibacterial therapy but also offers a promising strategy for treating chronic diabetic infections.
研究领域:
生物医学工程
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纳米技术
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微生物学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Ruiqi Zou,Junxiao Shi,Qi Lu,Chunyan Sun,Haiqing Ye,Xu Yan,Fangjie Tian,Hongxia Li
Given the potential dangers of organophosphorus pesticides to food safety and human health, the development of a reliable and precise detection platform for pesticides is essential. In this study, we present a novel ‘armor-plating’ laccase-mimetic catalyst (DNA-Cu@MOFs)-based colorimetric platform, which enables stable and selective pesticide detection. The DNA-Cu@MOFs enhance catalytic stability and overcome pH limitations, enabling effective catalysis under neutral and alkaline physiological conditions, making them well-suited for practical applications in biosensor development. By combining the catalytic properties of DNA-Cu@MOFs with a high-affinity biorecognition element (acetylcholinesterase), the platform achieves a linear detection range of 3.0–90 ng mL −1 for chlorpyrifos, with a detection limit of 0.75 ng mL −1 . Notably, this platform demonstrates significant stability in chlorpyrifos detection even in the presence of environmental interferents. This robust colorimetric platform offers new possibilities for pesticide detection and provides a solid foundation for the development of comprehensive and accurate pesticide monitoring systems.
研究领域:
生物传感
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环境科学
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食品科学
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DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 145
·期 14
·5032-5040
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IF 3.978
Jie Lv,Shuangling Wang,Cong Zhang,Yulong Lin,Yan Fu,Meng Li
Breaking the pH limitation of the enzyme-like activity of nanomaterials is of great importance for extending their applications in environmental and biomedical fields. Herein, to mimic the role of histidine residues in horseradish peroxidase (HRP), adenosine 5′-triphosphate (ATP) is reported to improve the peroxidase-like activity of hollow Prussian blue nanocubes (hPBNCs). Due to the inherited porous structures, hPBNCs can expose all the binding sites as far as possible to ATP to significantly amplify their catalytic activity and broaden their applicable pH range up to pH 12. Introduction of ATP provides the possibility of realizing efficient catalytic reactions under alkaline conditions. Upon binding with hPBNCs, ATP can enhance the stability of hPBNCs, increase the affinities of the catalysts towards substrates and improve the conductivity of hPBNCs as well as change the decomposed product from H2O2. Moreover, on the basis of the different catalytic activities of hPBNCs towards ATP, adenosine 5′-diphosphate and adenosine 5′-monophosphate, hPBNCs–ATP is utilized to construct a novel colorimetric sensor for the detection of alkaline phosphatase (ALP) activity in biological fluids, which is significantly important for the clinical diagnosis of ALP-related diseases.
研究领域:
纳米材料
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生物化学
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催化
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医学诊断
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产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY
·
2024
·
IF 4.3
Wenxin Bao,Luyuan Tian,Hao Wang,Aidong Tang,Huaming Yang
Overcoming the intrinsic low activity of most peroxidase mimics under neutral pH is crucial but still extremely challenging for the detection of disease markers in biological samples. Here, we chose nanoclay (i.e., montmorillonite K10, MK10) as a carrier to modulate the structure of Fe1–xS nanozyme components through an interfacial modulation strategy, aiming at breaking the neutral pH limitation of Fe1–xS. MK10 with abundant hydroxyl groups on its surface acts as a carrier to increase the ratio of Fe(II) and S(II−) content in surface Fe1–xS. We verify that Fe(II)-promoted surface hydroxyl radical generation and S(II−)-promoted regeneration of Fe(II) play key roles in endowing peroxidase-like activity to Fe1–xS at neutral pH. As expected, Fe1–xS/MK10 exhibited 11-fold higher Vmax and 52-fold higher catalytic efficiency than bare Fe1–xS. As a proof of concept, the sensor constructed based on Fe1–xS/MK10 achieved colorimetric detection of xanthine under neutral conditions with a linear range of 5–300 μM and a limit of detection of 2.49 μM. Finally, we achieved highly sensitive detection of xanthine in serum using the constructed biosensor. Our contribution is the novel use of a nanoclay-mediated interfacial modulation strategy for boosting the peroxidase-mimicking activity and breaking the pH limitation, which contributes to the in situ detection of disease markers by nanozymes under physiological conditions.
研究领域:
纳米技术
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生物传感器
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分析化学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xingxing Jiang,Shuping Liu,Xianwen Song,Qiang Li,Minghui Yang,Xiang Chen
How to overcome the intrinsic low activity of most oxidase and peroxidase mimics at neutral pH has been extremely challenging. Herein, we represent a chromium-mediated and ligand-dependent strategy to activate the oxidase-like activity of boron-doped g-C3N4 (B-g-C3N4, denoted as BG), aiming at breaking the pH limitation. Cr (III) can be in situ oxidized to Cr (IV) by generated •O2– upon UV light irradiation, which then works as a catalysis mediator to oxidize TMB under a neutral environment. Excitingly, the TMB oxidation can be rationally modulated by ligands on the BG coordinating with chromium. We verify that the PEI-Cr3+ coordination outperformed Cit-PEI-Cr3+ on the oxidase-like activity through a more accelerated electron transfer, unveiled by the Gauss theoretical calculations. This study highlights a paradigm of tuning the coordination environment on nanozyme surface via the ligand engineering strategy for boosting the oxidase-mimicking activity and breaking the pH limitation. Meanwhile, the catalysis-based colorimetric assay for accurate and selective identification of Cr3+ was achieved.
研究领域:
材料科学
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催化
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纳米技术
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分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Weiyi Lu,Shuyan Lou,Bin Yang,Meixuan Wu,Huimin Hao,Zihua Guo
The convenient monitoring of carbaryl is necessary because of its serious threat to environment and public health. In enzyme inhibition-based colorimetric detection, the oxidation of colorimetric substrate (3,3′,5,5′-tetramethyl benzidine) only proceeds at pH below 5, while most enzymes only work efficiently at neutral pH. Here fluorescein was doped in coordination polymers to serve as novel nanozymes for efficient light-activated oxidation. The long triplet state lifetime of Ce 3+ made the substrate oxidize at neutral pH to overcome a pH limitation. Furthermore, one interesting light-activated biosensing system was developed based on the carbaryl’s unique toxicity towards acetylcholinesterase. It can sensitively detect carbaryl at neutral pH with a limit of detection 1.53 μg/L, which is much lower than the maximum residue limit (50 μg/L) in European Union database. It also enjoys a good selectivity for carbaryl detection over other pesticides or endocrine disruptors . Besides, the biosensing system showed a satisfied performance to detect carbaryl in both water and food samples.
研究领域:
分析化学
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环境科学
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材料科学
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生物传感器
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）