JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2021
·Vol. 102
·期 5
·2070-2079
·
IF 3.639
Hang Liu,Junbing Zhang,Yong Xiong,Shengfeng Peng,David Julian McClements,Liqiang Zou,Ruihong Liang,Wei Liu
BACKGROUND Norbixin, a carotenoid extracted from annatto seeds, is widely utilized as a natural pigment in foods, cosmetics and medicines. Its water solubility is relatively high under neutral or alkaline conditions but low under acidic conditions, which limits its application in some food products. RESULTS This problem was overcome by utilizing liposomes to encapsulate the carotenoids so that they could be easily dispersed within acidic solutions. The norbixin was loaded into the liposomes using the pH-driven method. Liposomes were produced by passing aqueous phospholipid dispersions through a microfluidizer under high pressure. Norbixin was then added to the liposome dispersions at pH 7.0 and then driven into the hydrophobic domains of the phospholipid bilayers by acidifying the system. Measurements of the encapsulation efficiency showed that the norbixin was successfully loaded into the liposomes using the pH-driven method. X-ray diffraction analysis showed that the norbixin was in an amorphous state after incorporation into the liposomes. Encapsulation of norbixin within the liposomes was also shown to increase its water dispersibility and chemical stability under acidic pH conditions. CONCLUSION The pH-driven method therefore provides a useful means of increasing the application of this bioactive carotenoid within functional foods and other products. © 2021 Society of Chemical Industry.
研究领域:
食品科学
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生物技术
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化学工程
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Hanyu Li,Minghui Zhao,Jinghong Li,Jiaqi Wang,Huajiang Zhang,Jing Wang,Ning Xia,Zhongjiang Wang,Ahmed M. Rayan
The pH-driven method, recognized for its material safety and avoidance of organic solvents, is considered a promising approach for the encapsulation of hydrophobic active substances. However, the interaction mode and mechanism between carriers and receptors in two stages (alkalization and neutralization) remain underexplored. In this study, the hydrophilic globular protein ovalbumin (OVA) was utilized as a carrier to load curcumin (Cur) by the pH-driven method, with an alkalization pH of 8–12. The results indicated that the solubility of Cur and the degree of protein unfolding increased with increasing alkalization pH, with a transformation in the secondary structure observed at pH 12. During the alkalization stage at pH 11 and 12, the loading efficiency of Cur exceeded 90%, with binding constants reaching as high as 5.79 × 10 5 M −1 and 1.44 × 10 5 M −1 , respectively. The interactions transitioned through three phases in the alkalization process: electrostatic dominance to hydrophobic dominance, electrostatic assistance to hydrophobic dominance, and electrostatic repulsion hindrance. Notably, the generation of electrostatic repulsion and the potential loss of binding sites resulted in a decrease in the binding affinity between OVA and Cur at pH 12. In the neutralization stage, a stable structure dominated by hydrogen bonds and van der Waals forces was observed, with Cur encapsulated within the hydrophobic core of OVA. The mechanism proposed in this study elucidates the factors affecting the efficacy of the pH-driven method and helps to refine the application in hydrophobic active substance delivery systems.
研究领域:
食品科学
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生物化学
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药物递送系统
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yexuan Yao,Li Ma,Chengwei Yu,Ce Cheng,Hongxia Gao,Teng Wei,Litong Li,Zhiyue Wang,Wei Liu,Zeyuan Deng,Liqiang Zou,Ting Luo
To improve the poor water solubility and oral bioavailability of tyrosol, novel tyrosol liposomes (Tyr-LPs) were prepared by pH-driven method. Fourier transform infrared (FTIR) absorption spectra and X-ray diffraction (XRD) analysis indicated that Tyr-LPs were successfully encapsulated and tyrosol was in an amorphous state in liposomes. When tyrosol content in Tyr-LP was 1.33 mg/ml and the Tyr:LP (mass ratio) = 1:2, favorable dispersibility of Tyr-LP was exhibited, with an instability index of 0.049 ± 0.004, PDI of 0.274 ± 0.003, and the EE of 94.8 ± 2.5 %. In vivo pharmacokinetic studies showed that after oral administration of tyrosol or Tyr-LP (Tyr:LP = 1:2), concentration-versus-time curve (AUC 0–720mins ) and maximum concentration (C max ) values of Tyr-LP was respectively 1.5-fold ( P < 0.01) and 2.25-fold ( P < 0.01) higher than tyrosol, which indicated that the oral bioavailability of tyrosol was effectively improved in Tyr-LPs. Our study thereby provides theoretical support for the application of Tyr-LP for optimal delivery of tryosol.
研究领域:
药剂学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Hanyu Li,Minghui Zhao,Sijie Zhou,Huajiang Zhang,Jing Wang,Ning Xia,Yujia Liu,Shihui Hua,Guixin Tan
Curcumin is a hydrophobic polyphenol with various health benefits, but its poor water solubility and stability limit its application in the food and pharmaceutical industries. In this study, a pH-driven method was developed to prepare ovalbumin (OVA)-curcumin nanocomplexes without organic solvents, reducing the environmental impact. The results showed that curcumin was encapsulated in OVA with a high efficiency of over 95% and remained in an amorphous state within the protein molecules. Molecular docking and spectroscopic analyses revealed that curcumin was mainly bound and stabilized by hydrogen bonds and hydrophobic interactions in the hydrophobic core of OVA. The binding strength of the intermediate stage of the pH-driven method was stronger than that of the final stage, due to the exposure of OVA molecular binding sites and the involvement of electrostatic attraction. Furthermore, the potential of polysaccharide blending to enhance the stability of the nanocomplexes was investigated. The results showed that sodium alginate effectively enhanced the photostability and thermal stability of the OVA-curcumin nanocomplexes by co-solubilization and electrostatic complex formation. In addition, simulated in vitro digestion also demonstrated that the pH-driven method combined with polysaccharide blending increased the bioaccessibility of curcumin in nanocomplexes from 17.79% to 52.54%. Therefore, the pH-driven method coupled with polysaccharide blending ensures the encapsulation and stabilization of hydrophobic bioactive substances for use in their delivery systems to improve bioaccessibility.
研究领域:
食品科学
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药物化学
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生物技术
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yuanhao Sheng,Yufu Li,Huizhong Hu,Jinhong Zhong,Xin Zhong,Feilin Wang,Haihui Pan,Yongguang Bi,Fansheng Kong
Apigenin(AP) exhibits significant biological activities and pharmacological effects, including antioxidant, hypoglycemic, lipid-lowering, and anti-inflammatory properties. However, its application is limited by its low solubility, poor bioavailability, and instability. Gelatin is commonly used as a drug delivery carrier. In this study, apigenin-gelatin nanoparticles(AP-GNPs) were prepared using a pH-driven method to better explore the practical applications of AP. Characterization of AP-GNPs using FT-IR, XRD, SEM and TEM revealed that apigenin was bound to gelatin via hydrogen bonding and encapsulated within, forming a spherical amorphous structure.The particle size, PDI, zeta potential, encapsulation efficiency, and loading content of AP-GNPs were 365.3 ± 7.7 nm, 0.209 ± 0.022, −11.53 ± 0.82 mV, 83.7 ± 2.2 %, and 9.3 ± 0.3 %, respectively. Stability tests and gastrointestinal simulation digestion experiments demonstrated that AP-GNPs exhibited superior stability, solubility, and bioaccessibility compared to AP. Additionally, AP-GNPs provided protection against AAPH-induced erythrocyte haemolysis and showed inhibitory effects on the activities of α-glucosidase and α-amylase. In summary, the significant enhancement of the solubility, stability, and bioaccessibility of AP by AP-GNPs offers novel insights for the extensive application of apigenin in the pharmaceutical and food industries.
研究领域:
药学
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食品科学
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF MATERIALS PROCESSING TECHNOLOGY
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2025
·
IF 7.5
Fukun Li,Yang Bai,HaiXiang Hu,Guanbo Qiao,Lingzhong Li,Feng Zhang,Xuejun Zhang
Achieving atomic-level surface integrity while maintaining a high material removal rate (MRR) remains a fundamental challenge in the polishing of fused silica due to its high hardness and brittleness. This study establishes a novel mechanistic framework for understanding how pH-driven interfacial bond dynamics govern chemo-mechanical polishing processes. By integrating multiscale experimental characterization (XPS, FTIR, Raman spectroscopy) with ReaxFF molecular dynamics simulations, we demonstrate for the first time that the dynamic equilibrium between the formation and rupture of Ce–O–Si interfacial bonds directly control removal efficiency and surface quality. Alkaline conditions enhance OH – activity, facilitating Si–O bond hydrolysis and stable Ce–O–Si linkage formation, resulting in the highest MRR (397.6 nm/min). Acidic environments promote citrate-mediated Ce 3 + complexation and nanocluster dispersion, enabling a rolling mechanism that achieves ultra-smooth surfaces (Sq = 0.086 nm). In contrast, neutral pH conditions suffer from PEG adsorption blocking active sites, leading to a 62 % reduction in MRR relative to alkaline systems. A 12.2-fold increase in MRR compared to SiO 2 abrasives (32.67 nm/min) confirms that chemical interfacial dynamics, rather than mechanical abrasion alone, are critical to material removal. This fundamental advancement provides a broadly generalizable theoretical basis for designing efficient, low-damage precision polishing processes across oxide-based optical materials and beyond.
研究领域:
材料科学
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化学工程
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光学材料加工
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产品关联
DOI
高影响因子
Food Biophysics
·
2022
·Vol. 17
·期 3
·386-396
·
IF 3.34
Wang Yuhang,Fu Yuying,McClements David Julian,Ba Chujie,Li Teng
Propolis is a natural bioactive compound that is being explored as a nutraceutical because of its potential health benefits. In this study, propolis-loaded biopolymer nanoparticles were fabricated from folic acid-modified carboxymethyl chitosan (FA-CMCS) using the pH-driven method. The mean diameter of the propolis-loaded FA-CMCS nanoparticles was about 180 nm, and transmission electron microscopy showed they had a spherical shape. Fourier transform infrared spectroscopy analysis demonstrated that hydrogen bonding, electrostatic, and hydrophobic interactions were the major driving forces responsible for nanoparticle formation. The nanoparticles had good colloidal stability at pH 2.0, 3.0, 7.0, and 8.0 but exhibited some aggregation at intermediate pH values. The nanoparticles were also stable to salt addition up to 400 mM NaCl. Propolis-loaded FA-CMCS nanoparticles exhibited improved antioxidant properties compared to pure propolis and propolis-free nanoparticles. A simulated digestion model (fed state conditions) indicated that different amounts of propolis were released in different gastrointestinal regions: around 25%, 15%, and 25% in the stomach, small intestine, and colon, respectively. Our results suggest that the nanoparticles fabricated in this study have potential as colloidal delivery systems for sustained release of propolis in the human gut.
研究领域:
药剂学
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生物材料
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营养学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2020
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IF 5.065
Xiaojie Yu,Mo Li,Abu ElGasim A. Yagoub,Li Chen,Cunshan Zhou,Dong Yan
The function to induce dynamic-phase shifts between mono/biphasic systems is crucial to separation processes. Switchable (pH driven) ATPS composed of DESs and salts are here disclosed to act as integrated platforms for the production-separation processes. The catalytic carbohydrate conversion to 5-hydroxymethylfurfural (5HMF) was conducted in a homogeneous acidic aqueous-DES medium. Then, the formation of the biphasic region was induced by tailoring the pH of the aqueous homogeneous medium, leading to the complete separation of 5-HMF from the carbohydrate. Herein, the effects of the phase-forming DES species (composed of ethanol/n-propanol and [N 4444 ]Cl), the K 3 C 6 H 5 O 7 concentration and pH on the distribution of 5-HMF and fructose in DES-ATPSs were investigated. The optimal composition of the DES-ATPS system was 30 wt% [N 4444 ]Cl:ethanol (1:2) or [N 4444 ]Cl:n-propanol (1:2) and 35 and 40 wt% K 3 C 6 H 5 O 7 , respectively. The results showed that the integrated platforms have good catalytic effect, and the conversion rate of fructose reached 87.21%, with a 5-HMF yield of 46.76% and 0.1 g of apple peel produced 15.43 mg 5-HMF. The extraction efficiency of the DES-ATPS composed of [N 4444 ]Cl:ethanol (1:2) and [N 4444 ]Cl:n-propanol (1:2) were the highest within 20 min (5-HMF extraction efficiency 98.53% and 98.59%, respectively), which has economic benefits and comprehensiveness.
研究领域:
化学工程
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生物转化
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yanli Kong,Fan Lu,Aihua Huang,Zhonglin Chen,Jiangya Ma
Recent studies have predominantly focused on improving the oxidation capability of ferrate (Fe(VI)) by promoting the formation of Fe(IV)/Fe(V) through various activation methods. However, the low utilization rate of intermediate active iron species caused by the rapid self-decomposition of Fe(VI) limits its water treatment application. This study proposed to use covalently bonded aluminum-iron modified activated carbon (CAFM@PAC) to moderately activate Fe(VI) for carbamazepine (CBZ) degradation. The mutual interfacial between Fe(VI) and CAFM@PAC was explored, especially the mechanism of moderately activating to enhance the stability and utilization of activated intermediate iron and reactive oxygen species through metal modification modulation and pH-driven processes. Results showed that CBZ removal in Fe(VI)/CAFM@PAC system was higher than that of Fe(VI) alone system, especially in acidic conditions, which should be attributed to the enhanced interfacial reaction and electron transfer path through the synergistic effect of aluminum, iron and carbon. The interaction of Fe(VI) and CAFM@PAC had a moderate activating effect on Fe(VI) and inhibited its excessive self-decomposition, stabilizing and improving the utilization of active species. pH showed great influence on the high-valent iron species (Fe(IV)/Fe(V)) and reactive oxygen species (·OH, ·O 2 – , and 1 O 2 ) evolution and active species played different roles within varying pH. Moreover, the freshly generated rich aluminum-iron oxides enhance adsorption by effectively capturing and complexing CBZ degradation intermediate products. Fe(VI)/CAFM@PAC system possessed strong anti-interference ability and universality across a broad pH spectrum. The system integrated the advantages of highly active free radicals and selective metal active substances, achieving efficient removal of refractory organic pollutants. This study provided a new insight into the moderate activation effect for Fe(VI) by CAFM@PAC within dynamic pH and enriched the fundamental knowledge of Fe(VI) activation.
研究领域:
环境科学
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水处理技术
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产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 5
·1074
·
IF 4.35
Wei Zhou,Yun Zhang,Ruyi Li,Shengfeng Peng,Roger Ruan,Jihua Li,Wei Liu
Thymol has been applied as a spice and antibacterial agent in commercial products. However, the utilization of thymol in the food and pharmaceutical field has recently been limited by its poor water solubility and stability. In this work, a caseinate-stabilized thymol nanosuspension was fabricated by pH-driven methods to overcome those limitations. Firstly, the chemical stability of thymol at different pH value conditions was investigated. The physiochemical properties of thymol nanosuspensions were then characterized, such as average particle size, zeta potential, encapsulation efficiency, and loading capacity. Meanwhile, the X-ray diffraction results showed that thymol was present as an amorphous state in the nanosuspensions. The thermal stability of thymol was slightly enhanced by encapsulation through this process, and the thymol nanosuspensions were stable during the long-term storage, and the average particle size of nanosuspensions showed that there was no aggregation of nanosuspensions during storage and high temperature. Finally, the antimicrobial activity of thymol nanosuspensions was evaluated by investigating the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) againstSalmomella enterca,Staphlococcus aureus,Escherichia coli, andListeria monocytogenes. These results could provide useful information and implications for promoting the application of thymol in food, cosmetic, and pharmaceutical commercial products.Keywords:thymol;nanosuspensions;caseinate;pH-driven method;water solubility
研究领域:
食品科学
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药物学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2023
·
IF 10.7
Huishan Rao,Fei Chen,Lianzhu Lin,Mouming Zhao
Flavonoids have a variety of bioactivities, but their poor solubility, low bioavailability, and weak stability limit their application as functional factors in food systems. Soy extracts (SE) mainly composed of soy proteins and soluble polysaccharides at mass ratios of 4.1–5.8: 1 and possessing good bioactivity and nutritional property might be candidate functional encapsulation carriers to improve defects of flavonoids and enhance overall nutritional quality . In this study, galangin (Gal), a poorly water-soluble flavonoid, was used as an example to evaluate the application potential of SE in flavonoid delivery system. Gal-loaded SE nanoparticles (Gal-loaded SE NPs) with high Gal solubility, encapsulation efficiency and bioaccessibility were successfully constructed by pH-driven method. The solubilizing effect of SE on Gal largely depended on the concentrations of SE and Gal. Combining the encapsulation efficiency and retention rate, the optimal conditions were identified as SE concentration of 40 mg/mL, binding time of 5 min, and Gal to SE mass ratio of 1%. Gal-loaded SE NPs showed good storage, thermal, redissolution, and sodium ions stabilities. Based on Fourier transform infrared spectroscopy , X-ray diffraction, and transmission electron microscope analysis , the proteins in SE might undergo a typical de-folding and re-folding process during pH-shifting and mainly combine with Gal by hydrophobic interaction to form Gal-loaded SE NPs with spherical shape (particle diameter <100 nm). This study broadened the application of SE in flavonoid delivery system and provided new ideas for the design and development of new plant-based health food ingredients with multiple functional factors and high nutritional quality.
研究领域:
食品科学
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营养学
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生物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Xiaoting Liu,Limei Yu,Yu Fang,Weijia Zhang,Guanghui Li,Xiaofang Zeng,Yuanhong Zhang
This study aimed to evaluate the potential of pH-driven assembled soy peptide nanoparticle (SPN) to prepare high internal phase emulsions (HIPEs) containing sweet orange essential oil (SOEO), and the effects of SPN concentration and oil phase fraction on the formation, stability and flavor release characteristics were investigated. Results showed that stable HIPEs with excellent self-supporting state were successfully fabricated at relative high SPN concentrations (0.5–3.0 wt%). And the increase in SPN concentration could cause smaller droplet size, better viscoelastic properties and stability. The flavor release of SOEO in SPN-stabilized HIPEs could be slowed down and modulated by regulating SPN concentration, and the retention of key flavor compound ( d -limonene) in SOEO encapsulated in HIPEs could be reached to higher than 70 % after 120 days of storage. All these indicated the effective encapsulation and delivery of SOEO in SPN-stabilized HIPEs and their prospective application as fat substitutes in plant-based food systems.
研究领域:
食品科学
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生物材料
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）