PHARMAZIE
·
2020
·Vol. 75
·期 9
·412-416
·
IF 1.198
Yang Xiao-Guang,Li Yan-Feng,Wang Yan-Jun,Kong Rui-Heng,Wang Dun
Recent studies have shown that monoamine oxidase A (MAOA) is significantly expressed in malignant prostate cancer (PCa) and plays an important role in tumorigenesis indicating its potential to serve as a target for PCa treatment. Here, we choose the small molecule isoniazid as the MAOA
inhibition functionality and incorporated it in the tumor-targeting moiety of heptamethine carbocyanine dyes via a pH sensitive hydrazone bond to design and synthesize novel MAOA inhibitor isoniazid-heptamethine carbocyanine dye conjugates. Cytotoxicity assay in PC-3 cells shows that
all conjugates possessed improved antitumor efficacy compared with isoniazid. The tested compounds also demonstrated a moderate MAOA inhibitory effect. In conclusion, these results indicate that these conjugates exert antitumor effects by delivering the MAOA-inhibiting moiety to PCa cells.
研究领域:
药理学
·
肿瘤学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2024
·Vol. 40
·期 2
·287-292
·
IF 3.1
Li Yaohua,Wang Wei
Intracellular pH (pH i ) is a fundamental indicator of cellular physiological state, regulating cellular state and function, and has important research values. Although various probes for measuring intracellular pH were available, it is challenging to reflect pH i in real-time and reversible manners. Herein, we developed a whole-cell bioluminescent (BL) probe based on wild type BL bacteria, photobacterium phosphoreum ( P. phosphoreum ), to determine and image pH i . The dependence of BL intensity of P. phosphoreum on pH values of culture solutions was established. It was found that BL intensity could respond to the change of pH values rapidly and reversibly. We further revealed that P. phosphoreum maintained pH homeostasis in the extracellular pH (pH e ) within the range of 5.0–7.0, while intracellular pH homeostasis was destroyed at the alkaline pH e . This method opens up the enormous potential of BL bacteria as an alternative to fluorescence for monitoring and imaging pH i .
研究领域:
生物化学
·
细胞生物学
·
生物物理学
·
微生物学
·
生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Lu Shi,Feifei Cao,Limin Zhang,Yang Tian
The development of switches responding to specific pH changes was particularly useful in wide application fields. Owing to flexible switches simulated by pH, i-motif DNAs are widely used as a pH sensor. But its character of structure transition strongly dependent on acidic pH severely hampers the application of i-motif DNA in physiological media. Herein, we report the stable i-motif structure formed at a physiological pH triggered by spatial confinement of silica nanochannels. Three classic DNA chains containing 21-mer i-motif domain base-pairs and a single-stranded multiply (T)n spacer, 5′-COOH-(T)n-CCCTAACCCTAACCCTAACCC-3′, were employed to evaluate the enhanced stability of i-motif structure. Compared to their free states in a dilute solution, the transition pH of all i-motif DNAs decorated in nanochannels remarkably shifts toward a neutral pH. Moreover, the transition midpoint can be tuned sensitively over the physiologically relevant pH range through slightly varying the length of T base spacer. Density functional theory (DFT) calculations validate that the increased proton density in a nanochannel triggers the formation of an i-motif structure under a neutral pH. Finally, this i-motif DNA based nanochannels electrode was successfully employed to monitor pH in brain microdialysates followed by cerebral ischemia. The present approach is not limited by fundamental investigation for DNA conformation but may extend toward the manipulation of i-motif based structures for artificial molecular machines and signaling systems.
研究领域:
生物化学
·
分子生物学
·
医学检测
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Huan Yu,Mingtai Sun,Kui Zhang,Houjuan Zhu,Zhengjie Liu,Yajun Zhang,Jun Zhao,Lijun Wu,Zhongping Zhang,Suhua Wang
The intracellular pH value controls many aspects of cell metabolism and is considered as an integral part of many metabolic reactions by maintaining the structure and function of nearly all proteins. Detection of pH fluctuation covering the whole intracellular pH window including both neutral and acidic is essential but challenging and still needs more sensitive pH probes. We have synthesized a new near-infrared fluorescent and colorimetric probe based on cyanine dye, which can be used for monitoring the pH fluctuation. The probe has a good water solubility, excellent photostability, reversibility, low cytotoxicity and exhibits a good performance to detect pH fluctuation in both solution and live cells. Upon incubation with live cells, it responds sensitively to low pH value in cell medium by exhibiting bright red fluorescence. On the contrary, the fluorescence intensity of probe seems to be very weak in neutral cellular environment. The satisfactory results show that the probe was a highly sensitive pH probe in living cells and further demonstrate its promising biological applications.
研究领域:
化学
·
生物学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Xiao Long Xiong,Yun Peng Ma,Hui Liu,Cheng Zhi Huang,Jun Zhou
The determination of pH values is crucial in various fields, such as analytical chemistry, medical diagnostics, and biochemical research. pH test strips, renowned for their convenience and cost-effectiveness, are commonly utilized for pH qualitative estimation. Recently, quantitative methods for determining pH values using pH test strips have been developed. However, these methods can be prone to errors due to environmental factors, such as lighting conditions, which affect the imaging quality of the pH test strips. To address these challenges, we developed an innovative approach that combines machine learning techniques with pH test strips for the quantitative determination of pH values. Our method involves extracting artificial features from the pH test strip images and combining them across multiple dimensions for comprehensive analysis. To ensure optimal feature selection, we developed a feature selection strategy based on SHAP importance. This strategy helps in identifying the most relevant features that contribute to accurate pH prediction. Furthermore, we integrated multiple machine learning algorithms, employing a robust stacking fusion strategy to establish a highly reliable pH value prediction model. Our proposed method automates the determination of pH values through pH test strips, effectively overcoming the limitations associated with environmental lighting interference. Experimental results demonstrate that this method is convenient, effective, and highly reliable for the determination of pH values.
研究领域:
分析化学
·
生物信息学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2019
·Vol. 10
·期 1
·21-28
·
IF 3.049
Yongqian Li,Yunlong Mao,Chi Xiao,Xiaoli Xu,Xueyong Li
pH is a critical parameter used to specify the acidity or alkalinity of an aqueous solution in chemistry, food processing, and medical care. In this study, a conductimetric-type micro pH sensor has been achieved using PANI membrane fabricated on a flexible substrate film aiming to monitor wound healing. The sensor is based on the incorporation of a polyaniline (PANI) membrane, interdigital electrode, and polyimide (PI) substrate. PANI was doped with dodecyl benzene sulfonic acid (DBSA) to obtain good conductivity. The electrodes were patterned on the PI film by etching. The contact area between the PANI and interdigital electrodes improves the responsiveness of the pH sensor. A sensitivity of 58.57 mV per pH over the entire pH range from 5.45 to 8.62 was obtained experimentally, along with a superior repeatability of 8% FS (full scale) and a temperature drift of 6.8% FS. This micro flexible pH sensor aims to monitor the pH value of wound healing, which also facilitates the realization of online monitoring of the pH for telemedicine, food safety, and home health care.
研究领域:
化学传感器
·
医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2010
·
IF 3.898
Ming Fang,Jiang Long,Weifeng Zhao,Liwei Wang,Guohua Chen
Homogeneous aqueous suspensions of graphene have been prepared by chemical reduction of graphene oxide in the presence of chitosan. The graphene sheets in thus prepared suspensions can be switched reversibly between a well dispersed and a more aggregated state with pH as a stimulus.
研究领域:
材料科学
·
生物医学工程
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Haixin Tan,Bin Chen,Meihuan Liu,Jiamiao Jiang,Juanfeng Ou,Lu Liu,Fei Wang,Yicheng Ye,Junbin Gao,Jia Sun,Fei Peng,Yingfeng Tu
Light-driven micro/nanomotors have made enormous progress in various practical applications owing to their excellent controllability and programmability. However, it is still far from real intellectualization. Herein we demonstrate the successful fabrication of hollow cuprous oxide (Cu 2 O) micromotors with autonomous phototaxis reversal behavior by in-situ sensing the local pH change of surrounding environment without altering the lighting direction. The fabricated motors exhibit excellent and controllable propulsion under low-strength visible light illumination (1100 LUX) due to faster charge separation between different facets of the crosslinked polyhedral Cu 2 O microstructures. Intriguingly, our Cu 2 O micromotors are possible to respond smartly to the local pH gradient and reverse from negative to positive phototaxis, therefore the motors are capable of evacuating automatically after mission. We envision that such adaptive micromotor with great intellectualization via responding autonomously to the local stimuli shows great promise in various applications especially biomedical scenarios.
研究领域:
纳米技术
·
生物医学工程
·
材料科学
·
机器人技术
✓ 已验证
DOI
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2014
·Vol. 21
·期 7
·1-8
·
IF 1.897
Wu Wei,Wang Weigang,Li Shuai,Wang Jiantao,Zhang Qiujing,Li Xiuhua,Luo Xianglin,Li Jianshu
In order to develop polymeric self-assembly with an intelligent morphological transition property under appropriate stimuli, a series of pH-sensitive amphiphilic block copolymers have been prepared via atom transfer radical polymerization (ATRP). Due to the delicately designed macromolecular structure, poly(ethylene glycol) 44 - b -poly(2-diisopropylaminoethyl methacrylate) 15 (PEG 44 - b -PDPA 15 ) could form self-assemblies with a controllable morphological transition in response to the physiological pH changes. As demonstrated by the results of dynamic light scattering (DLS) records and transmission electron microscopy (TEM) micrographs, PEG 44 - b -PDPA 15 could self-assemble into toruloid aggregates arranged by several single micelles at pH 7.4, but the morphology changed into uniform single micelles at pH 6.5. With a further decrease of pH value to 5.5, PEG- b -PDPA became double hydrophilic and could not self-assemble into any nanostructure. Therefore, this block copolymer provides a feasible approach to construct a nanoscale smart self-assembly with adjustable morphologies, which exhibits its potential for biomedical applications with specific physiological pH stimuli, such as intracellular delivery and tumor therapy.
研究领域:
生物材料学
·
高分子化学
·
生物医药工程
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Huihui Yang,Jun Chen,Yuling Liang,Yanfei Zhang,Wen Yin,Yuzhi Xu,Si-Yang Liu,Zong Dai,Xiaoyong Zou
Dynamic imaging of cell surface pH is extremely challenging due to the slight changes in pH and the fast diffusion of secreted acid to the extracellular environment. In this work, we construct a novel metal–organic framework (MOF)-shell-confined i-motif-based pH probe (MOFC-i) strategy that enables sensitive and dynamic imaging of cell surface pH. The CY3- and CY5-labeled i-motif, which is hybridized via its short complementary chain with two-base mismatches, is optimized for sensing at physiological pH. After efficiently anchoring the optimized pH probes onto the cell membrane with the aid of cholesterol groups, a biocompatible microporous MOF shell is then formed around the cell by cross-linking ZIF-8 nanoparticles via tannic acid. The microporous MOF shell can confine secreted acid without inhibiting the normal physiological activities of cells; thus, the MOFC-i strategy can be used to monitor dynamic changes in the cell surface pH of living cells. Furthermore, this method can not only clearly distinguish the different metabolic behaviors of cancer cells and normal cells but also reveal drug effects on the cell surface pH or metabolism, providing promising prospects in pH-related diagnostics and drug screening.
研究领域:
生物化学
·
细胞生物学
·
分子生物学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Chunmin Qiu,Guoqiang Wu,Puying Zhao,Junbiao Dai,Qian Luo,Liqing Zhao,Guobin Mao,Yingxin Ma
Background High-precision and broad-range pH detection is critical for health status assessment, such as signal transduction, enzyme activity, endocytosis, and cell proliferation and apoptosis. Although pH-responsive ratiometric fluorescent probes offer an effective pH monitoring strategy, their preparation often requires multi-step modification and decreases fluorescence efficiency and stability. Herein, we developed a simple method to prepare fluorescent Si dots with dual emission centers for high-precision and broad-range pH monitoring, and the detection of urease based on pH-responsive Si dots and pH monitoring in living cell was further explored. Results The dual-emitting Si dots had two emission centers at 427 nm and 500 nm. The emission at 427 nm showed a fluorescence enhancement and effective quenching in the pH ranges of 3.0-10.0 and 10.0-12.0, respectively. While the emission at 500 nm increased gradually with increasing pH from 3.0 to 12.0. Two linear relationships were obtained between the synchronous fluorescence intensity ratio and pH in the ranges of 7.0-9.5 and 10.0-12.0 with a △pH of 0.1, indicating a broad detection range and high precision. Then the Si dots were used to detect urease activity via urea hydrolysis-mediated pH changes. A linear range of 1-80 U/L was established with a detection limit of 0.28 U/L. Furthermore, the Si dots were used for pH imaging in living HeLa cells. The cells changed from green to blue when the pH of HeLa cells increased from 6.0 to 10.0. Significance These findings collectively suggest that the intrinsic dual-emitting Si dots may offer a simple and versatile platform for developing pH-relevant biosensors and bioimaging applications. Additionally, this approach provides reliable methods for preparing intrinsic dual-emitting probe and constructing fluorescence ratiometrics, which widely used in health status assessment.
研究领域:
分析化学
·
生物化学
·
生物物理学
·
生物传感器
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2022
·
IF 4.831
Yutao Jia,Yanting Shen,Yanyan Zhu,Jing Wang
Lysosomes are the acidic organelles in the cells that play an important role in intracellular degradation and other various cellular functions. The pH disturbance of lysosomes will result in the lysosomal dysfunction and many lysosomal related diseases. In this work, we reported a methoxy-based covalent organic framework (TAPB-DMTP-COF) that a novel pH-responsive fluorescent probe for lysosomal pH imaging in cells. The prepared TAPB-DMTP-COF presented regular crystal structure, low toxicity and good pH responsive property. The rich imine structure in the material enabled pH-responsive properties of the TAPB-DMTP-COF and made it exhibited pH-dependent fluorescence response. Good detection linearity for pH measurements in aqueous solution was achieved by this probe. Moreover, the TAPB-DMTP-COF can be used for the selective lysosomal pH imaging. Confocal fluorescence imaging results demonstrated that the pH fluctuations (from 4.0 to 7.4) and the pH changes in lysosomes can be effectively monitored in situ by the developed probe. This study may provide a new avenue for the intracellular pH sensing, deep study and understanding about the mechanism of diseases related to abnormal lysosomal pH.
研究领域:
生物化学
·
材料科学
·
医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）