FEBS Open Bio
·
2020
·Vol. 10
·期 12
·2733-2739
·
IF 2.231
Sai-sai Ding,Jin-peng Zhu,Yang Wang,Bin Wu,Zongpei Zhao
In the paper industry, chlorine is often used to treat the pulp for bleaching. After pulping, a large amount of xylan is present in the fiber. Xylanase can be used to degrade xylan in an eco-friendly process called biobleaching, which can help minimize the usage of chlorine in the delignification process. However, a bottleneck in the adoption of biobleaching is the cost of xylanase and the requirement that xylanase be active and stable at extreme conditions. Here, we investigated whether using sodium alginate beads to immobilize an extracellular xylanase from Bacillus subtilis (Lucky9) can reduce the potential cost of enzyme usage. The optimal pH and the activity of the immobilized enzyme were increased at optimal temperature compared with the free enzyme. In addition, immobilized xylanase was shown to be more stable than free xylanase. The results of this study suggest that the immobilized xylanase has potential applications in the biobleaching industry.
研究领域:
生物技术
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酶工程
·
制浆造纸工业
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PubMed
高影响因子
Journal of Analytical Science and Technology
·
2021
·Vol. 12
·期 1
·1-12
·
IF 2.769
Chen Kangni,Li Yanbo,Shang Jinyan,Li Heping
Biothiols play important roles in various physiological and biological processes, which closely related to many diseases. Hydrazine is widely used in the chemical industry, but it is harmful to humans and animals. Therefore, it is very important to develop a fluorescent probe that can simultaneously detect biological thiols and hydrazine. In this work, a new fluorescent probe (2E,4Z)-2-(benzo[d]thiazol-2-yl)-5-chloro-5-(7-(diethylamino)-2-oxo-2H-chromen-3-yl)penta-2,4-dienenitrile (BCD) was synthesized by integrating coumarin and benzothiazole acetonitrile. Featured with four binding sites and different bonding mechanism between probe with biothiols and hydrazine, this probe exhibited fluorescent turn-on for distinguishing Cys, Hcy, GSH and hydrazine with 760-, 8-, 6- and 637-fold fluorescent intensity increase at 502, 479, 476 and 458 nm, respectively, through different excitation wavelengths. Research on the effect of pH on the fluorescent performance of BCD shows that the probe exhibits superior stability in a weakly alkaline to weakly acidic environment, which will facilitate the detection of biological thiols or hydrazine hydrate by BCD. Selectivity studies have shown that the probe has high specificity to biological thiols and hydrazine, which is of great significance to the application of BCD.
研究领域:
分析化学
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生物化学
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高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2024
·
IF 3.7
Yuhang Wei,Qingpeng Zhu,Weiwei Xie,Xinyue Wang,Song Li,Zhiming Chen
Efficient and convenient treatment of industrial dyeing wastewater is of a great significance to guarantee human and animal health. This work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on amino-functionalized ZnFe 2 O 4 nanoparticles (ZnFe 2 O 4 -laccase) and its application for degradation of textile dyes. Due to the existence of a large number of oxygen vacancies on the surface of the ZnFe 2 O 4 nanoparticles, negative ions accumulated on the magnetic carriers, which resulted in a harsh optimal pH value of the ZnFe 2 O 4 -laccase. Laccase activity assays revealed that the ZnFe 2 O 4 -laccase possessed superior pH and thermal stabilities, excellent reusability, and noticeable organic solvent tolerance. Meanwhile, the ZnFe 2 O 4 -laccase presented efficient and sustainable degradation of high concentrations of textile dyes. The initial decoloration efficiencies of malachite green (MG), brilliant green (BG), azophloxine, crystal violet (CV), reactive blue 19 (RB19) and procion red MX-5B were approximately 99.1%, 95.0%, 93.3%, 87.4%, 86.1% and 85.3%, respectively. After 10 consecutive reuses, the degradation rates of the textile dyes still maintained about 98.2%, 92.5%, 83.2%, 81.5%, 79.8% and 65.9%, respectively. The excellent dye degradation properties indicate that the ZnFe 2 O 4 -laccase has a technical application in high concentrations of dyestuffs treatment.
研究领域:
环境科学
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生物技术
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高影响因子
Marine Drugs
·
2024
·Vol. 22
·期 6
·287
·
IF 4.9
Han-Zhong Guo,Dou Wang,Hui-Ting Yang,Yu-Le Wu,Yong-Cheng Li,Guang-Hua Xia,Xue-Ying Zhang
To promote the bioconversion of marine chitin waste into value-added products, we expressed a novel pH-stableMicromonospora aurantiaca-derived chitinase,MaChi1, inEscherichia coliand subsequently purified, characterized, and evaluated it for its chitin-converting capacity. Our results indicated thatMaChi1 is of the glycoside hydrolase (GH) family 18 with a molecular weight of approximately 57 kDa, consisting of a GH18 catalytic domain and a cellulose-binding domain. We recorded its optimal activity at pH 5.0 and 55 °C. It exhibited excellent stability in a wide pH range of 3.0–10.0. Mg2+(5 mM), and dithiothreitol (10 mM) significantly promotedMaChi1 activity.MaChi1 exhibited broad substrate specificity and hydrolyzed chitin, chitosan, cellulose, soluble starch, andN-acetyl chitooligosaccharides with polymerization degrees ranging from three to six. Moreover,MaChi1 exhibited an endo-type cleavage pattern, and it could efficiently convert colloidal chitin intoN-acetyl-D-glucosamine (GlcNAc) and (GlcNAc)2with yields of 227.2 and 505.9 mg/g chitin, respectively. Its high chitin-degrading capacity and exceptional pH tolerance makes it a promising tool with potential applications in chitin waste treatment and bioactive oligosaccharide production.
研究领域:
酶学
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微生物学
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生物化学
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DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Jiuhong Zhao,Jinnan Zhao,Xiaoyu Zhang,Guixia Ling,Peng Zhang
The level of dopamine (DA) in the human body has a certain correlation with neurological diseases. However, most detection methods of DA are complex and expensive. In this study, laccase-like DNAzyme@MOF with improved pH stability was successfully prepared. DNAzyme@MOF could catalyze the chromogenic substrate to change the color of the solution for the detection of DA in ISF. The addition of DNAzyme made DNAzyme@MOF possess higher stability and enzyme-like activity. The operation process was simple, rapid, and intuitive. In addition, the in vivo DA content in skin interstitial fluid (ISF) was analyzed by an off-line method. The swelling hydrogel microneedles (MNs) were prepared to extract skin ISF. DA in skin ISF was recovered and detected by laccase-like DNAzyme@MOF. This study realized the minimally invasive detection of DA. The proposed detection method of biomarkers in ISF based on DNAzyme@MOF would provide a new dimension towards the future development for the detection of other biomarkers in ISF.
研究领域:
生物化学
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医学检测
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纳米材料科学
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DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-9
·
IF 14.7
Su Yafei,Peng Huawen,Liu Xufei,Li Jiapeng,Zhao Qiang
Cation separation under extreme pH is crucial for lithium recovery from spent batteries, but conventional polyamide membranes suffer from pH-induced hydrolysis. Preparation of high performance nanofiltration membranes with excellent pH-resistance remains a challenge. Here we synthesize a high performance nanofiltration membrane (1,4,7,10-Tetraazacyclododecane (TAD)−1,3,5-Tris(bromomethyl)benzene (TBMB) thin film composite membranes (TFCMs)) with excellent pH-stability through interfacial quaternization reaction between TAD and TBMB. Due to the high stability of “C-N” bonds in TAD-TBMB TFCMs, its separation performance is stable even after 70 days immersion in concentrated acid (3 M H 2 SO 4 , HNO 3 , or HCl) and base (3 M NaOH), which is at least 15 times more stable than benchmark commercial membranes. The membrane shows an overall separation performance (11.3 L m −2 h −1 bar −1 (LMHB), R Co2+ : 97% in 2 M H 2 SO 4 ) due to the size sieving and the intensified charge repulsion, outperforming many of the state-of-the-art membranes. Finally, the TAD-TBMB TFCM remains stable during 30-days continuous nanofiltration of 2 M H 2 SO 4 and leachate (2 M H 2 SO 4 , ions: 6.2 g L −1 ) from spent batteries.
研究领域:
材料科学
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环境工程
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DOI
PubMed
高影响因子
Analytical Methods
·
2025
·
IF 2.6
Yanan Zhang,Yimin Wang,Ningjing Huang,Wei zheng,Hang Guo,Chunyin Li
A sensor for non-enzymatic hydrogen peroxide (H2O2) was developed based on silicon quantum dots (SiQDs). The fluorescent SiQDs were synthesized using a one-pot hydrothermal method. The SiQDs were made water-soluble and pH-stable by covering their surfaces with amino and hydroxyl groups. Additionally, the prepared SiQDs had a large Stokes shift (131 nm), excellent photostability (442 min), and hypotoxicity (e.g., 99% of cell viability was kept after a 24 h-treatment at 8.0 mg/mL of the SiQDs). The dynamic quenching effect induced by H2O2 effectively suppresses the blue fluorescence of SiQDs. Under the optimum conditions, the SiQD-based H2O2 sensor exhibited a wide linear range (10–1000 μM), high sensitivity and selectivity, and a good anti-interference ability. The detection limit and relative standard deviation were 1.7 μM and 4.5% (c = 20 μM, n = 7), respectively. Accordingly, a simple, enzyme-free, and highly efficient method was established for detecting H2O2 in aqueous solutions. Moreover, this eco-friendly fluorescent sensor successfully determined H2O2 in human serum samples with 92%–102% recoveries.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2025
·
IF 4.2
Xiangning Fang,Guanyu Jiang,Qinglong Qiao,Xiaogang Liu,Zhaochao Xu
Rhodamines are widely utilized for fluorescence switching, biosensing, and super-resolution imaging due to their dynamic equilibrium between a non-fluorescent "closed" spiro-ring form and a fluorescent "open" xanthylium form. This equilibrium has inspired extensive efforts to chemically modulate their spiro-ring behavior. In this study, we uncover a novel mechanism in aniline-rhodamine ( PR ), where the interplay of NH protonation, deprotonation, and hydrogen bonding collaboratively governs spirolactone ring opening. Notably, this interplay stabilizes the ring equilibrium in aqueous solutions within the pH range of 4–10, minimizing sensitivity to pH fluctuations. Leveraging this mechanism, we designed an aniline-rhodamine derivative, EPR , which maintains stable fluorescence regardless of lysosomal pH variations. This stability enables consistent, high-resolution imaging of lysosomal dynamics under both physiological and pathological conditions, offering deep insights into lysosomal functions within cellular regulatory networks. The NH groups in PR establish a new regulatory axis for rhodamine ring switching, providing a promising foundation for developing next-generation fluorescent probes with enhanced stability and functionality.
研究领域:
细胞生物学
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分子成像
·
化学生物学
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生物医学工程
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超分辨显微技术
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2017
·Vol. 41
·期 14
·6646-6652
·
IF 3.269
Ke Zhang,Lingling Luo,Wenjie Li,Honglin Zhang,Yi Zhang,Jiupeng Zhao,Yao Li
The preparation of polystyrene (PS) beads loaded with platinum octaethylporphyrin (PtOEP) via a swelling method has been well incorporated into dissolved oxygen (DO) sensing detection. The experimental results showed that the highest value of I0/I100 was 7.26 and the loading ratio of PtOEP was 51.33%; the luminescent intensity declined by only 2.23% under UV light for 1 hour. The response and recovery times were 16 s and 180 s, respectively. The peak position and intensity of the emission spectrum in buffer solutions with different pH values did not change. This indicates that this oxygen sensor has excellent sensitivity, light stability, reversibility, and pH resistance. Moreover, on this basis, we incorporated another oxygen insensitive dye, coumarin 6 (C6), to prepare a ratiometric oxygen sensor, which showed high sensitivity towards O2 with superior quenching efficiency. Finally, this oxygen sensor was used to monitor the content of DO in fish tank, tap water, and the Songhua River. All the measured results were well within the reference range.
研究领域:
生物化学
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材料科学
·
环境监测
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2018
·
IF 6.434
Binbin Shao,Zhifeng Liu,Guangming Zeng,Yang Liu,Xin Yang,Chengyun Zhou,Ming Chen,Yujie Liu,Yilin Jiang,Ming Yan
In this study, the hollow mesoporous carbon spheres (HMCs) were synthesized and modified for laccase (Lac) immobilization, and the structural characteristics of HMCs materials were determined by FESEM, TEM and FTIR etc. The maximum loading of Lac on the HMCs materials could reach 835 mg/g, meanwhile, the immobilized Lac exhibited excellent thermo-stability, pH stability, storage stability and reusability. The antibiotics removal experiments indicated that the immobilized Lac possess efficient removal efficiency for both tetracycline hydrochloride (TCH) and ciprofloxacin hydrochloride (CPH) in the presence of redox mediator. The synergy of the adsorption by HMCs and the degradation by Lac could be the reasons for the high removal of antibiotics. Meanwhile, for investigating degradation mechanism, the degradation product analysis and molecular docking method had been introduced to this study. According to the degradation products, dehydroxylation and demethylation are major degradation reactions for TCH degradation, and the oxidation of the piperazinyl substituent and hydroxylation are the major degradation for CPH degradation. The docking results showed that some important residues played the key role in the degradation process. This study indicated that the immobilization of Lac on HMCs could be potentially applied in environmental remediation of antibiotics.
研究领域:
环境科学
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生物技术
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材料科学
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jiahui Wang,Zixian Shen,Xianfeng Ma,Wenjing Ding,Hua Wang,Mingqi Wang,Xiaofeng Yu
Mitochondria, the primary energy-producing organelles, critically regulate cellular activities. Their internal viscosity—a key microenvironmental parameter—directly affects material transport and energy conversion, with aberrant levels linked to disease pathogenesis. Most reported mitochondrial viscosity probes were susceptible to polar interference, resulting in insufficient specificity for viscosity detection. This study designed and developed a novel polar-insensitive fluorescent probe, Y-CN , based on a phenothiazine skeleton - a small molecular compound with excellent fluorescent properties. The experimental data revealed an excellent linear relationship (R 2 > 0.99) between Y-CN 's fluorescence intensity and viscosity over a wide range from 1.005 to 219 cP. When viscosity increased from 1.005 cP (aqueous buffer) to 219 cP, Y-CN demonstrated a dramatic 209-fold fluorescence amplification. Furthermore, the probe exhibited excellent selectivity, pH stability, and low toxicity. Moreover, Y-CN could detect viscosity changes induced by food thickeners. Y-CN successfully monitored dynamic changes in mitochondrial viscosity within live cells, providing a powerful tool for specific investigation of mitochondria-associated physiological and pathological processes, with significant potential for biological applications.
研究领域:
生物化学
·
细胞生物学
·
生物物理
·
荧光成像
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分子探针
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
·
2017
·Vol. 17
·期 1
·370-376
·
IF 1.483
Xia Guo-Hua,Liu Wei,Jiang Xiao-Ping,Wang Xiang-Yu,Zhang Ye-Wang,Guo Jianyong
Magnetic Fe3O4 nanoparticles were prepared through hydrothermal method and coated with silica on the surface to obtain Fe3O4@SiO2 core–shell nanoparticles. After modification with different functional groups including aldehyde,
amine and diimide, the nanoparticles were used as carrier for covalent immobilization of lipase. The nanoparticles with aldehyde groups showed highest immobilization yield (52.8%) and efficiency (86.5%). And the immobilization conditions including pH, temperature and the concentration of enzyme
were optimized. After immobilization, the K
m of lipase was altered from 2.3 to 3.2 mM. The thermal stability and pH stability were enhanced by immobilization at the investigated conditions: pH 5.0–8.0 and temperature 30–70 °C. After 10 batches conversion of
4-Nitrophenyl palmitate into p-Nitrophenol, the immobilized lipase retained over 75% of the original activity. Compared with the commercial lipase Novozym435, the immobilized lipase showed better stability and higher catalytic efficiency. These results demonstrate that the immobilized lipase
on the modified Fe3O4@SiO2 magnetic nanoparticles has enhanced stability and reusability, which make lipase of potential interest in a number of industrial applications.
研究领域:
生物化学
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纳米材料科学
·
酶工程
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）