IEEE SENSORS JOURNAL
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2024
·
IF 4.3
Zhenpeng Men,Lucan Xiao,Ragini Singh,Bingyuan Zhang,Santosh Kumar
In this work, a sensitive and portable fiber-optic WaveFlex biosensor is developed for the detection of paraoxon pesticide in agricultural products. A combination of simulation and experiment is used to analyze the distribution of the evanescent field in optical fiber. The flexible W-shaped optical fiber biosensor (OFB) based on localized surface plasmon resonance (LSPR) effect is developed, also known as WaveFlex biosensor. Gold nanoparticles (AuNPs) were immobilized on the surface of the optical fiber probe to successfully excite the LSPR phenomenon. In addition, in order to modify the optical fiber probe, cerium dioxide nanorods (CeO2-NRs) and tungsten disulfide quantum dots (WS2-QDs) were functionalized. It is used to enhance the biocompatibility of the sensor and increase the specific surface area of the fiber probe. The enzymatic determination of organophosphorus pesticides (OPs) is usually based on cholinesterase and organophosphorus hydrolase. Paraoxon has an irreversible inhibitory effect on butyrylcholinesterase. The content of paraoxon was detected by the inhibitory effect of paraoxon on butyrylcholinesterase. The reproducibility, reusability, stability, selectivity, and pH tests of the W-shaped WaveFlex sensor and its application in real samples are evaluated using different concentrations of paraoxon solution. The linear range of the sensor is 0– $100~\mu $ g/mL, and the limit of detection (LoD) is $7.999~\mu $ g/mL. The sensitivity is as high as 0.0312 nm/( $\mu $ g/mL), which verifies that the sensor provides an indispensable technology, cost-effective and portable solution for paraoxon-specific detection.
研究领域:
环境科学
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生物传感技术
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农业科学
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DOI
高影响因子
Analytical Methods
·
2021
·Vol. 13
·期 47
·5727-5734
·
IF 2.896
Mengyao Li,Lulu Guo,Lili Chen,Chunhua Lin,Li Wang
Methyl paraoxon (MP) has attracted more and more attention in recent years because of its severe neurotoxicity and respiratory toxicity. Therefore, it is very urgent to develop new and sensitive MP detection methods for health protection and public safety. Covalent organic frameworks (COFs) are widely used in fluorescence detection because they can effectively transmit and amplify probe signals with multiple identical binding sites within an extended framework. Here, COFML-DHTA nanosheet material was synthesized by the solvothermal reaction of melem (ML) and 2,5-dihydroxyterephthalaldehyde (DHTA). The resulting COFML-DHTA exhibits remarkable luminescence quenching toward MP due to the relationship of competitive absorption and Förster resonance energy transfer between MP and COFML-DHTA. COFML-DHTA can be used for sensitive and selective detection of MP in a wide concentration range of 0.57 ng mL−1 to 30 μg mL−1 with a detection limit of 0.19 ng mL−1. The material has good chemical stability, excellent selectivity, good reusability and hydrophilicity, which provide more possibilities for COFs in the detection of pesticides.
研究领域:
材料科学
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环境科学
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毒理学
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DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Shaoyang Liu,Mai Luo,Huizhen Liang,Liang Zou,Jiliang Cao,Jinchao Wei,Peng Li
Organophosphorus pesticide residues pose significant risks to human health, but conventional analytical methods are costly and complex. To address this, we developed a new environmentally friendly technique using a simple hydrothermal method to create a ratiometric fluorescent composite (RFC) with two distinct signals: blue and orange. This RFC allowed for rapid detection and quantitative analysis of organophosphorus pesticides. The composite incorporated a cerium-based metal–organic framework (Ce-UiO-66), which could degrade methyl paraoxon (MP) due to the two oxidation states of cerium. The resulting degradation product, p-nitrophenol (p-NP), specifically quenched the blue fluorescence of the RFC via the inner filter effect (IFE), while the orange fluorescence remained unaffected, ensuring consistency under microenvironmental and spectrometer conditions. This innovative approach was sensitive, with a detection limit of 0.55 μM, selective, and repeatable, offering a new strategy for pesticide analysis and safety evaluation of edible and medicinal plant and water samples.
研究领域:
环境科学
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分析化学
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食品安全
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产品关联
DOI
高影响因子
Journal of Advanced Research
·
2025
·
IF 13
Mai Luo,Jihai Cai,Sijia Pu,Ting Wang,W. Sijia,Mengwei Wang,Shuangpeng Wang,Songnan Qu,Liang Zou,Peng Li,Jinchao Wei
Introduction Pesticide residues pose a significant threat to human health. Inner filter effect (IFE) stands out for its ability to enable the rapid and cost-effective establishment of fluorescent detection systems. Nevertheless, the practical implementation is often hindered by issues such as low spectral overlap and interference from impurities, which can compromise its effectiveness. Objectives To develop novel AIEE-type CDs and apply them to improve the efficiency of IFE-based strategies. With the aid of AIEE-type CDs, we aim to develop a multi-channel detection platform that provides more reliable fluorescent probes for real sample and imaging applications. Methods Preparation of N, S-CDs was achieved using a novel combination of sources. The activation mechanism of Supra-CDs’ AIEE characteristics was verified. In the methodological experiments, detection performance of Supra-CDs was tested to confirm their improvement in the IFE-based strategy, practical applicability for real sample detection, and imaging capability. Results In this work, p-PA and thiourea were used for the first time to prepare N, S-CDs. After adding water, the fluorescence intensity of Supra-CDs increased by nearly 360 % compared to N, S-CDs. Spectral overlap rates of the target and probe in the controllable IFE-based system improved from 1.80 % to 33.11 %, indicating superior analytical performance in aqueous solutions compared to ethanol. The fluorescent method exhibited a linear range of 0.5–100 μM, with a detection limit of 0.5 μM. Furthermore, a smartphone-based system has been proposed for the colorimetric analysis of methyl paraoxon, offering a more convenient and efficient alternative. Additionally, Supra-CD demonstrated exceptional capabilities in cell imaging, specifically targeting intracellular lipids within HepG2 cells. Conclusion This study unveils a new type of Supra-CDs that exhibit AIEE property. It also introduces a creative fluorescent sensor with tunable IFE. In summary, this research delivers important insights into AIEE-type Supra-CDs and affirms their potential to inspire broader applications.
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Mai Luo,Jinchao Wei,Yunyang Zhao,Yuzhou Sun,Hengxing Liang,Shuangpeng Wang,Peng Li
Methyl-paraoxon (MP) is a representative organophosphorus pesticide (OP), which is a huge threat to the safety of human beings. In this study, carbon dots doped with boron and nitrogen (B, N–CDs) were used as a fluorescent probe for MP detection. The prepared cerium oxide (CeO 2 ) decompose MP efficiently into p-nitrophenol (p-NP) and phosphate group in the alkaline condition. The dissociative p-NP could reduce the fluorescent intensity of B, N–CDs through the inner filter effect (IFE). The pH value, incubation time, the amount of B, N–CDs and solvent effect were investigated and optimized for detection system. Under the optimum conditions, the limit of the detection was calculated to be 24.7 ng mL −1 with simple procedures. Tap water, lake water, Semen Coicis and Panax quinquefolius L. samples were used to test the recoveries of the detection system. The recoveries ranged from 94.68% to 107.28%. Furthermore, the fluorescence laminate was developed by painting B, N–CDs on the polyamide laminate. The MP residue could be observed directly under the 365 nm lamp, and linearity for MP by using the fluorescent laminates was ranged from 0.015 to 1.5 mM. The present work provides a rapid and dual-signal based method (fluorescent and visual) for routine detection of MP in real samples.
研究领域:
分析化学
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环境科学
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食品安全
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Mai Luo,Ling Chen,Jinchao Wei,Xiping Cui,Zehua Cheng,Ting Wang,Incheng Chao,Yunyang Zhao,Hao Gao,Peng Li
Exogenous pollution of Chinese medicinal materials by pesticide residues and heavy metal ions has attracted great attention. Relying on the rapid development of nanotechnology and multidisciplinary fields, fluorescent techniques have been widely applied in contaminant detection and pollution monitoring due to their advantages of simple preparation, low cost, high throughput and others. Most importantly, synchronous detection of multi-targets has always been pursued as one of the major goals in the design of fluorescent probes. Herein, we firstly develop a simultaneous sensing method for methyl-paraoxon (MP) and Nickel ion (Ni, Ⅱ) by using carbon based fluorescent nanocomposite with ratiometric signal readout and nanozyme. Notably, the designed system showed excellent effectiveness even when the two pollutants co-exist. Under the optimum conditions, this method provides low limits of detection of 1.25 µM for methyl-paraoxon and 0.01 µM for Ni (Ⅱ). To further verify the reliability, recovery studies of these two analytes were performed on ginseng radix et rhizoma, nelumbinis semen, and water samples. In addition, smartphone-based visual analysis has been introduced to expand its applicability in point of care detection. This work not only expands the application of the dual-mode approach to pollutant detection, but also provides insights into the analysis of multiple pollutants in a single assay.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2018
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IF 6.735
Xiao-hui Wei,Chao Li,Chunlai Wang,Song Lin,Jinhui Wu,Min-jie Guo
Hierarchical organic-inorganic nanocomposites derived from polyethersulfone (PES) nanofibrous membranes with a post-modification of dopamine (DA) and polyethylenimine (PEI), comprising a high content (33 wt%) of MgO nanoparticles of smaller dimensions (12.7 ± 3.0 nm), were successively fabricated using electrospinning and DA/PEI self-polymerization methods. The obtained hybrid membranes have a rapid capture and great destructive capacity towards paraoxon-ethyl toxin degradation with a relatively short interaction time (up to 92% within 40 min) for effective environmental remediation.
研究领域:
环境科学
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材料科学
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毒理学
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产品关联
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 9
·1429
·
IF 5.719
Shuo Liu,Peng Zhang,Yuming Miao,Chenmin Li,Yu-e Shi,Jinhua Liu,Yun-kai Lv,Zhenguang Wang
Selective and sensitive identification of paraoxon residue in agricultural products is greatly significant for food safety but remains a challenging task. Herein, a detection platform was developed by integrating Cu nanoclusters (Cu NCs) with MnO2nanosheets, where the fluorescence of Cu NCs was effectively quenched. Upon introducing butyrylcholinesterase and butyrylcholine into the system, their hydrolysate, thiocholine, leads to the decomposition of the platform through a reaction between the MnO2nanosheets and thiol groups on thiocholine. The electron-rich groups on thiocholine can further promote the fluorescence intensity of Cu NCs through host–guest interactions. Adding paraoxon results in the failure of fluorescence recovery and further promotion, which could be utilized for the quantitative detection of paraoxon, and a limit of detection as low as 0.22 ng/mL can be achieved. The detection platform shows strong tolerance to common interference species, which endows its applications for the detection of paraoxon in vegetables and fruit. These presented results not only open a new door for the functionalization of metal nanoclusters but also offer an inspiring strategy for analytic techniques in nanomedicine and environmental science.Keywords:organophosphorus pesticides;fluorometry assay;metal nanoclusters;MnO2nanosheets;fluorescence quenching
研究领域:
食品安全
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分析化学
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纳米医学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
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2022
·
IF 7.968
Meijie Cai,Shisheng Liu,Junjie Cai,Lei Zhao,Xiaohui Wei,Minjie Guo,Song Lin
A combination microwave-assisted hydrothermal and calcination method was used to produce serial mesoporous [email protected] ( [email protected] ) hybrid nanocomposites, which were studied and validated by TEM, XPS, XRD, and Raman spectra . The calcined [email protected] nanocomposites have a high specific surface area and small grain sizes with an enhanced capacity to degrade paraoxon after optimal preparation. The optimal [email protected] nanocomposites was obtained by the calcining temperature at 600 °C, and the particle size was about 6.4 ± 2 nm with a higher specific surface area of 255.6 m 2 /g. At room temperature, the decomposed amount of paraoxon toxins by [email protected] is ca. 55.65 mg/ [email protected] min. Furthermore, the pseudo-second-order kinetic model (R 2 > 0.99) fits the experimental degradation data better. The enhanced degradation effect of [email protected] was verified due to the generations of more superoxide radical (·O 2 - ) and hydroxyl radical (·OH) active sites. A photothermal effect was also observed in the [email protected] sample. The removal efficiency increased 8-fold after being exposed to near-infrared (NIR) radiation at 808 nm compared to the non-irradiating sample. Because of their ease of manufacture, low cost, and high removal effectiveness, [email protected] nanocomposites could be a suitable adsorbent choice for eliminating organophosphorus hazardous contaminants.
研究领域:
材料科学
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环境科学
·
纳米技术
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催化
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毒理学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenjing Li,Mengqing Xu,Zhuoyun Xia,Zheng Wang,Yingying Feng,Hui Li,Chuang Jiang,Yingnan Liu
The highly selective and sensitive detection of paraoxon residues at low environmental concentrations remains challenging. Herein, a novel fluorescent metal-organic framework composite (Ce 4+ @UiO-66-NH 2 ) with excellent phosphatase-like activity was developed to catalyze the hydrolysis of paraoxon into yellow p -nitrophenol ( p -NP), which quenched the composite's blue fluorescence via the inner filter effect. Based on this mechanism, a simple, portable, non-biological colorimetric/fluorescent hydrogel nanosensor (CM-SA spheres) was constructed by incorporating red fluorescent Mn-CDs with aggregation-induced emission as an internal reference. Upon increasing paraoxon concentration, the spheres showed visible color changes from colorless to yellow under daylight and blue to red under UV light. The sensor demonstrated a visual detection sensitivity of 0.05 μg mL −1 and limits of detection of 0.013 μg mL −1 (daylight) and 0.027 μg mL −1 (UV). In real sample analyses of tea and water, the CM-SA spheres exhibited strong anti-interference capability and reliability, offering a practical strategy for paraoxon degradation and detection.
研究领域:
环境监测
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分析化学
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纳米技术
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传感器开发
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食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2019
·
IF 4.474
Lingpu Jia,Yaxin Zhou,Kaipeng Wu,Qingling Feng,Chunming Wang,Ping He
A flexible acetylcholinesterase (AChE) film biosensor, based on a AuNPs-MoS 2 -reduced graphene oxide/polyimide flexible film (rGO/PI) electrode, has been synthesized for paraoxon detection. In this study, the rGO/PI film acts as the flexible substrate and AuNPs are reduced by monolayer MoS 2 under illumination. Transmission electron microscopy revealed that AuNPs are uniformly dispersed on the MoS 2 -rGO/PI electrode surface with a diameter ~10 nm. X-ray photoelectron spectroscopy indicated that a strong binding force exists between reduced AuNPs and monolayer MoS 2 . The AChE modified AuNPs-MoS 2 -rGO/PI flexible film biosensor is used to hydrolyze acetylcholine chloride and obtain a large current response at 0.49 V by differential pulse voltammetry , demonstrating successful immobilization of AChE. In view of the inhibition of paraoxon on the AChE, under optimal conditions, the AChE/AuNPs-MoS 2 -rGO/PI film biosensor shows a linear response over a concentration range 0.005–0.150 μg/mL, a sensitivity of 4.44 uA/μg/ mL, a detection limit of 0.0014 μg/mL, acceptable reproducibility and stability to paraoxon. The flexible film biosensor has also proved used for detection of paraoxon in real samples.
研究领域:
材料科学
·
分析化学
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生物传感器
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环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2020
·
IF 2.673
Yuzhou Sun,Jinchao Wei,Jian Zou,Zehua Cheng,Zhongming Huang,Liqiang Gu,Zhangfeng Zhong,Shengliang Li,Yitao Wang,Peng Li
A new electrochemical sensor for organophosphate pesticide (methyl-paraoxon) detection based on bifunctional cerium oxide (CeO 2 ) nanozyme is here reported for the first time. Methyl-paraoxon was degraded into p -nitrophenol by using CeO 2 with phosphatase mimicking activity. The CeO 2 nanozyme-modified electrode was then synthesized to detect p -nitrophenol. Cyclic voltammetry was applied to investigate the electrochemical behavior of the modified electrode, which indicates that the signal enhancement effect may attribute to the coating of CeO 2 nanozyme. The current research also studied and discussed the main parameters affecting the analytical signal, including accumulation potential, accumulation time, and pH. Under the optimum conditions, the present method provided a wider linear range from 0.1 to 100 μmol/L for methyl-paraoxon with a detection limit of 0.06 μmol/L. To validate the proof of concept, the electrochemical sensor was then successfully applied for the determination of methyl-paraoxon in three herb samples, i.e., Coix lacryma-jobi, Adenophora stricta and Semen nelumbinis. Our findings may provide new insights into the application of bifunctional nanozyme in electrochemical detection of organophosphorus pesticide.
研究领域:
分析化学
·
环境科学
·
农药检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）