CARBOHYDRATE POLYMERS
·
2020
·
IF 7.182
Yihua Yin,Mengdan Yang,Jing Xi,Weiquan Cai,Ying Yi,Guanghua He,Yue Dai,Ting Zhou,Mengqing Jiang
To improve in vitro photostability and enhance insecticidal activity, a novel esterase/glutathione (GSH) responsive photoactivated nano-pesticide delivery system was synthesized by conjugation of photoactivated pesticide phloxine B(PB) to sodium alginate (SA) via esterase/GSH sensitive phenolic ester bond followed by ultrasonic dispersion. The system was stable in PBS (pH 7.4) and could protect effectively the conjugated PB from in vitro photodegradation because of aggregation-caused quenching effect, whose maximum photodegradation rate did not exceed 10 % after 270 min illumination. However, upon exposure to esterase-6 or GSH stimulus, high photoactivity was observed due to the destruction of the system and accompanied by PB release. The combined stimulation could trigger more PB release than any single stimulus and thus resulting in a higher photoactivity. Compared with free PB, The system showed a higher phototoxicity on Sf9 insect cells and the in vitro light exposure had little influence on the phototoxicity.
研究领域:
生物化学
·
农药科学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Yu Ling,Zhong Feng Gao,Qian Zhou,Nian Bing Li,Hong Qun Luo
A multidimensional optical sensing platform which combines the advantages of resonance Rayleigh scattering (RRS), fluorescence, and colorimetry has been designed for detection of heparin. Phloxine B, a fluorescein derivative showing the special RRS spectrum in the long wavelength region, was selected to develop an easy-to-get system which can achieve switch-on sensing to obtain high sensitivity. The noise level of RRS in the long wavelength region is much weaker, and the reproducibility is much better; in this way, the sensitivity and selectivity can be improved. In the absence of heparin, the phloxine B and polyethyleneimine (PEI) form a complex through electrostatic interaction. Thus, the RRS signal at 554 nm is low; the phloxine B fluorescence is quenched, and the absorption signal is low. In the presence of heparin, competitive binding occurred between phloxine B and heparin toward PEI; then, phloxine B is gradually released from the phloxine B/PEI complex, causing obvious enhancement of the RRS, fluorescence, and absorption signals. Besides, the desorption of phloxine B is less effective for the heparin analogues, such as hyaluronic acid and chondroitin sulfate. In addition, the system presents a low detection limit of heparin to 5.0 × 10–4 U mL–1 and can also be applied to the detection of heparin in heparin sodium injection and 50% human serum samples with satisfactory results. Finally, the potential application of this method in reversible on–off molecular logic gate fabrication was discussed using the triple-channel optical signals as outputs.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Mingguang Ma,Jing Lu,Ling Wang,Xia Qiu,Tingting Xu,Sufan Wang,Yinggui Zhu
In this work, we first reported the electrochemiluminescence (ECL) properties of 2-hydroxynicotinic acid (2-HNA) with 0.09 M K 2 S 2 O 8 in 0.5 M H 3 BO 3 – Na 2 B 4 O 7 buffer solution. The effects of the type of co-reactant, the concentration of K 2 S 2 O 8 and the pH of the buffer solution on the ECL intensity of this system were investigated. And the possible mechanism of 2-HNA/ K 2 S 2 O 8 system has been speculated by fluorescence spectrum and ECL spectrum. Besides, we found that phloxine B (PB) can significantly enhance the ECL intensity of the 2-HNA/ K 2 S 2 O 8 system. It is speculated that an electrochemiluminescence resonance energy transfer (ECL-RET) process may occur between 2-HNA and PB, so that PB enhanced the ECL of the 2-HNA system. Combining the ultraviolet spectrum and density functional theory (DFT) calculations further proved the rationality of this speculation. Based on this, a new method of ECL detection of PB with high sensitivity and high selectivity was established.
研究领域:
分析化学
·
电化学
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Haiyun Zhai,Zihao Su,Zuanguang Chen,Zhenping Liu,Kaisong Yuan,Lu Huang
A method was developed to sensitively determine phloxine B in coffee bean by molecularly imprinted polymers (MIPs) coated graphene oxide (GO) solid-phase extraction (GO-MISPE) coupled with high-performance liquid chromatography and laser-induced fluorescence detection (HPLC–LIF). The GO-MISPE capillary monolithic column was prepared by water-bath in situ polymerization, using GO as supporting material, phloxine B, methacrylic acid (MAA), and ethylene dimethacrylate (EDMA) as template, functional monomer, and cross-linker, respectively. The properties of the homemade GO-MISPE capillary monolithic column, including capacity and specificity, were investigated under optimized conditions. The GO-MIPs were characterized by scanning electron microscopy (SEM) and Fourier transform-infrared spectroscopy (FT-IR). The mean recoveries of phloxine B in coffee bean ranged from 89.5% to 91.4% and the intra-day and inter-day relative standard deviation (RSD) values all ranged from 3.6% to 4.7%. Good linearity was obtained over 0.001–2.0 μg mL −1 ( r = 0.9995) with the detection limit (S/N = 3) of 0.075 ng mL −1 . Under the selected conditions, enrichment factors of over 90-fold were obtained and extraction on the monolithic column effectively cleaned up the coffee bean matrix. The results demonstrated that the proposed GO-MISPE HPLC–LIF method can be applied to sensitively determine phloxine B in coffee bean.
研究领域:
分析化学
·
食品安全
·
分离科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）