FOOD CHEMISTRY
·
2024
·
IF 8.5
Jiani Zhang,Xiaohui Guo,Jian Zhang,Xiaobing Guo,Yuying Xu,Lijuan Chen
Phoxim, extensively utilized in agriculture as an organothiophosphate insecticide, has the potential to cause neurotoxicity and pose human health hazards. In this study, an electrochemical enzyme biosensor based on Ti 3 C 2 MXene/MoS 2 @AuNPs/AChE was constructed for the sensitive detection of phoxim. The two-dimensional multilayer structure of Ti 3 C 2 MXene provides a robust framework for MoS 2 , leading to an expansion of the specific surface area and effectively preventing re-stacking of Ti 3 C 2 MXene. Additionally, the synergistic effect of self-reduced grown AuNPs with MoS 2 further improves the electrical conductivity of the composites, while the robust framework provides a favorable microenvironment for immobilization of enzyme molecules. Ti 3 C 2 MXene/MoS 2 @AuNPs electrochemical enzyme sensor showed a significant response to phoxim in the range of 1 × 10 −13 M to 1 × 10 −7 M with a detection limit of 5.29 × 10 −15 M. Moreover, the sensor demonstrated excellent repeatability, reproducibility, and stability, thereby showing its promising potential for real sample detection.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF INSECT SCIENCE
·
2021
·Vol. 21
·期 1
·
IF 1.857
Sheng Sheng,Wang Jiao,Zhang Xiao-rui,Liu Zhi-xiang,Yan Meng-wen,Shao Ying,Zhou Jin-cheng,Wu Fu-an,Wang Jun
Insecticides can have consequences for beneficial arthropods. Insect parasitoids can contact insecticides through direct exposure spray droplets or residues on crop foliage. Here, we focus on better understand the response of Meteorus pulchricornis (Wesmael), a parasitoid wasp of lepidopteran pests, and its detoxification mechanisms on stress caused by phoxim and cypermethrin. Hence, we determined the dose–mortality curves and estimating the sublethal concentrations (LC30 and LC50). Then, we applied the sublethal concentrations against adult parasitoids to assess its survival, parasitism efficacy, and also developmental and morphometric parameters of their offspring. Simultaneously, we check the activities of glutathione S-transferase (GST), acetylcholinesterase (AChE), and peroxidase (POD) after sublethal exposure of both insecticides, which has measured until 48 h after treatment. Overall, phoxim and cypermethrin exhibited acute lethal activity toward the parasitoid with LC50 values 4.608 and 8.570 mg/liter, respectively. Also, we detect that LC30 was able to trigger the enzymatic activity of GST, AChE, and POD, suggesting a potential detoxification mechanism. However, even when subjected to sublethal exposure, our results indicate strong negatives effects, in particular for phoxim, which has affected the parasitism efficacy and also the developmental and morphometric parameters of M. pulchricornis offspring. Therefore, it can be concluded that both phoxim and cypermethrin have negative impacts on M. pulchricornis and we suggest cautioning their use and the need for semifield and field assessments to confirm such an impact.
研究领域:
昆虫学
·
生态毒理学
✓ 已验证
DOI
PubMed
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 11
·4786-4792
·
IF 1.821
Xuecai Tan,Jiawen Wu,Qi Hu,Xiaoyu Li,Pengfei Li,Huicheng Yu,Xiaoyan Li,Fuhou Lei
Graphene and a novel cross-linker (ethylene glycol maleic rosinate acrylate) for preparing highly sensitive, molecularly imprinted sensors were proposed for phoxim determination. A molecularly imprinted polymer (MIP) film was created on a graphene-modified electrode for the determination of phoxim using a free radical polymerization method. The electrochemical properties of the MIP and non-molecularly imprinted polymer (NIP) sensors were investigated via cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The surface morphology of the imprinted film was characterized by scanning electron microscopy (SEM). Under the optimal experimental conditions, the peak currents were proportional to the concentrations of phoxim in the range of 8.0 × 10−7 to 1.4 × 10−4 mol L−1 with a detection limit of 2.0 × 10−8 mol L−1 (S/N = 3). The imprinted electrochemical sensor was employed to determine phoxim in cucumber samples with recovery ranging from 98.06% to 101.13%. An adsorption model for the imprinted sensor was investigated, and the measured imprinting factor β of the sensor was found to be 7.23, with the binding rate constant k being 14.2247 s. The developed electrochemical sensor based on the graphene modified electrode and the molecularly imprinted polymer exhibited good repeatability and stability, and can be successfully used to determine phoxim in cucumber samples.
研究领域:
分析化学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2021
·
IF 3.963
Zhi-Xiang Liu,Xiao-Rong Xing,Xin-Hao Liang,Jian-hao Ding,Yi-Jiangcheng Li,Ying Shao,Fu-An Wu,Jun Wang,Sheng Sheng
Glyphodes pyloalis Walker is a destructive pest on mulberry trees and poses a significant threat to the sericultural industry in China. Phoxim and chlorfenapyr are two commonly used insecticides in mulberry fields. Glutathione-S-transferases (GSTs) comprise a multifunctional protein superfamily that plays important roles in the detoxification of insecticides and xenobiotic compounds in insects. However, whether GSTs participate in the tolerance of phoxim and chlorfenapyr in G. pyloalis is still unknown . To better understand the mechanism of insecticide tolerance in G. pyloalis , the enzymatic activity of GSTs was evaluated under phoxim and chlorfenapyr exposure, respectively. GST enzyme activity was significantly increased after 12, 36 and 48 h of phoxim treatment and 12, 24, 36 and 48 h of chlorfenapyr treatment. Subsequently, eighteen GST genes were identified from the larvae transcriptome of G. pyloalis . Among these, ten GpGSTs had GSH-binding sites and fifteen GpGSTs had variable hydrophobic substrate-binding sites. The expression levels of Delta-GpGST and Epsilon-GpGST genes were significantly influenced by phoxim and chlorfenapyr treatment, and by the time post insecticide application . Furthermore, after silencing GpGST-E4 , the mortality rate of G. pyloalis larvae was increased when they were exposed to chlorfenapyr, but it did not significantly alter when the larvae were exposed to phoxim. Our results indicated the vital roles of GpGSTs in the tolerance of insecticides and this action depends on the categories of insecticides. The present study provides a theoretical basis for elucidating insecticide susceptibility and promotes functional research on GST genes in G. pyloalis .
研究领域:
昆虫学
·
分子生物学
·
害虫防治
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xiaoyu Su,Zhiyu Chen,Huan Wang,Lei Yuan,Kaiyi Zheng,Wen Zhang,Xiaobo Zou
A ratiometric electrochemical immunosensor, based on DNA tetrahedron nanostructure (DTNS), is introduced for vegetable phoxim determination. DTNS spontaneously adheres onto gold-nanoparticle-modified electrode and forms stable three-dimensional structure, providing plenty of binding sites to the built-in reference, methylene blue (MB). Monoclonal antibody (m-Ab) is vertically linked onto DTNS vertex, selectively responses antigenic phoxim, and promotes the target signal of I PHO . Thus, a ratiometric indicator, I PHO / I MB , is sensibly established with the target signal ( I PHO ) and the reference signal ( I MB ). Modifications, mechanisms and advances of the proposed method are subsequently examined with morphological methods and electrochemical experiments. This method brings considerable advances in analytical behaviors. The ratiometric signal presents better performance than solo system in repeatability and long-time stability. As-fabricated sensor presents wide dynamic range as 0.1∼30 μg/L, and limit of detection is well defined as 0.003 μg/L (S/N = 3). Finally, this method is verified with real-vegetable-sample analysis, certified HPLC and recovery test.
研究领域:
分析化学
·
食品科学
·
生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Xinyue Huang,Lei Jiang,Lin Yuan,Wensi Ma,Xintao Cui,Jiaxuan Liu,Xiuling Ji,YujiaoTu
Pesticides are chemicals widely utilized in various industries, particularly agriculture, to manage pests and enhance crop yields. However, the excessive use of pesticides, such as phoxim, poses threats to the environment, food safety, and human health. Thus, it is crucial to use pesticides judiciously, limiting their application to what is necessary. Fluorescent probes have garnered attention for their cost-effectiveness, straightforward preparation, and high sensitivity, positioning them as promising candidates for detecting phoxim. In this context, orange ratiometric fluorescent carbon dots were synthesized using rhodamine B and NaOH as precursors through a hydrothermal method. The synthesized Rh B Na CDs were then purified via dialysis, thin-layer chromatography, and chromatographic columns to develop an accurate and precise ratiometric fluorescent probe. This nanoprobe demonstrated excellent linear ranges for detecting phoxim levels in various food samples. It exhibited a low detection limit of 4.486 nM for phoxim concentrations ranging from 0 to 25 μM and can accurately detect concentrations between 25 and 250 μM. Moreover, it proved sensitive in identifying phoxim content in samples of red grapes, green grapes, tomatoes, and pitayas.
研究领域:
分析化学
·
环境科学
·
食品科学
·
纳米技术
·
农药检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2024
·
IF 3.9
Mengting Huang,Yuan Ma,Qianru Fan,Shunli Che,Jun Zhang,Shuquan Ding,Shuren Zhu,Xilei Li
Nanopolystyrene (NP) and phoxim (PHO) are pervasive environmental contaminants that pose a significant threat to the health of aquatic organisms, prompting widespread concern among researchers and the public alike. The hepatopancreas play important roles in the Chinese mitten crab ( Eriocheir sinensis ), such as digestion, absorption and detoxification. This study assessed the hepatopancreatic toxicity caused by the exposure of Eriocheir sinensis to environmentally relevant concentrations of NP and/or PHO. After a 21-day exposure period, NP (1.0 × 10 10 particles/L) and PHO (24 μg/L) exposure resulted in reduced number of blister-like, resorptive, and fibrillar cells and an elevation in lipid droplets within the hepatopancreas compared to the control group. Furthermore, trypsin and lipase activity decreased, amylase activity increased, and a significantly decrease in the expression of digestion-related genes, including CHT , CarL , and CarB , suggested impairment in both digestive and metabolic functions. The marked upregulation of key genes, including PPARγ , GYK , PEPCK , and SCD , as well as key metabolites such as 4-methylzymosterol-carboxylate, zymosterone, lathosterol, 7-dehydro-desmosterol, vitamin D2, 24-methylene-cycloartanol, 5-dehydroepisterol, and sitosterol in the lipid metabolic pathway, showed that the peroxisome proliferator-activated receptor (PPAR) and steroid biosynthesis signaling pathways were highly affected by exposure to NP and/or PHO. These findings indicated that exposure to NP and/or PHO might adversely affect the hepatopancreatic physiological homeostasis in E. sinensis by causing tissue damage and interfering with digestive and metabolic functions. Our results provide ecotoxicological insights into the effects of nanopolystyrene and/or phoxim exposure on the digestive function of Eriocheir sinensis .
研究领域:
环境科学
·
生态毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Zhenjun Xiao,Junlong Zhang,Yijie Lin,Kaixuan Zheng,Zili Lin,Mengyang Niu,Wanlin Lin,Yuchun Hong,Zhongyang Li,Ping Chen,Qingbin Song,Wenying Lv,Guoguang Liu
Developing new technologies to address emerging contaminants are urgently needed. To this end, a novel advanced oxidation system (US/CaSO 3 /PDS) utilizing ultrasound to activate persulfate (PDS) and calcium sulfite (CaSO 3 ) for the effective degradation of emerging contaminants was constructed in this study. The pseudo first-order kinetics constant of Phoxim (PHO) can reach 4.3 × 10 −2 min −1 in US/CaSO 3 /PDS system, revealing the prospects for the treatment of pollutants in water. Electron paramagnetic resonance spectra demonstrated that persulfate anion radicals, hydroperoxyl radicals, hydroxyl radicals, and singlet oxygen were produced in the system. Further, quenching experiments and reactive species probes were used to verify the chemical reaction mechanism of this novel system, and the experimental results highlight the key role of 1 O 2 in the degradation of pollutants thanks to the participation of oxygen. In addition, 11 main products in the PHO degradation process were identified by liquid chromatography-high-resolution precise mass spectrometry, and the possible degradation path of PHO in US/CaSO 3 /PDS system was proposed. US/CaSO 3 /PDS system had high pollutant removal efficiency against a variety of emerging pollutants and was applicable in real water matrix. This study provides a new scheme for the development of advanced oxidation technology based on PDS activation for the removal of refractory emerging pollutants in water.
研究领域:
环境科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2026
·
IF 6.6
Qing Li,Xiaohui Xue,Shun Zou,Bin He
Phoxim, a typical organophosphorus pesticide widely used in agriculture and aquaculture, poses significant ecotoxicological risks to fish species due to its environmental residues. In this study, we focused on the liver, the core detoxification organ, systematically investigated the transcriptomic responses of Carassius auratus exposed to different concentrations (low, medium, high) of phoxim for 24 h and 96 h. Integrating WGCNA with qRT-PCR validation, we elucidated the dynamic, stage-specific toxic mechanisms and adaptive responses. The results demonstrated that phoxim toxicity exhibits marked time-dependent accumulation and concentration dependence. A total of 30,540 differentially expressed genes (DEGs) were enriched at 24 h (defense phase), increasing to 38,320 DEGs at 96 h (adaptation phase). With increasing concentration, the number of DEGs decreased at 24 h but increased at 96 h. GO and KEGG enrichment analyses revealed that 24 h exposure triggered broad cellular stress and defense responses, whereas 96 h exposure shifted toward concentration-dependent pathological processes: low concentrations continuously activated detoxification and tissue remodeling pathways, medium concentrations were dominated by sustained signaling pathway perturbations, and high concentrations exhibited energy depletion and amino acid metabolic disorders. WGCNA identified functionally distinct co-expression modules, constructing a dynamic model spanning homeostasis (Cyan module), defense (Blue, Green, and Purple modules), and adaptation (Yellow module). qRT-PCR validation of key CYP450 isoforms showed concentration- and time-dependent induction patterns, with sustained high expression even at 96 h under high concentration, corroborating the significant enrichment of the drug metabolism-cytochrome P450 pathway in the Yellow module. This study presents comprehensive analysis of time- and concentration-dependent phoxim effect on C. auratus liver cells gene transcription modulation.
✓ 已验证
DOI
PubMed
高影响因子
Food Analytical Methods
·
2014
·Vol. 7
·期 9
·1729-1736
·
IF 1.802
Chao Mingyong,Chen Meifeng
Employing a graphene-modified glassy carbon electrode, a sensitive and convenient electrochemical method for the determination of phoxim by linear sweep voltammetry was developed. The electrochemical behavior of phoxim at the modified electrode was studied by cyclic voltammetry. In citric acid–phosphate buffers, the modified electrode exhibited excellent electrocatalytical effect on the reduction of phoxim and this was further used for the determination of phoxim. Under optimized analytical conditions, the reduction peak current showed a linear relationship with the concentration of phoxim in a range of 5.97 to 5,966 μg L −1 , with a correlation coefficient of 0.9993 and a detection limit of 2.39 μg L −1 . The proposed method shows excellent sensitivity, selectivity, and linearity and has been successfully applied for the determination of phoxim in a variety of food samples with satisfactory results.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Wenhui Hao,Gengli Huang,Guoyong Jiang,Sa-adu Abiola Dauda,Fuwei Pi
To control and on-site evaluate the contamination of organophosphorus pesticides, one direct sensing approach based on spontaneous fluorescence resonance energy transfer (FRET) was developed in this study. The luminescent metal-organic frameworks (LMOFs), a novel achievement of MOFs, could provide adjustable adsorption capacity and fluorescence index to scientific analysis was first employed to evaluate phoxim molecules in vegetables and fruits. Through the π-π recognition and FRET sensing between phoxim molecules and skeleton of Nu-1000 LMOFs probes, an ultra-low detection limit of 1.65 pg/L in the range of 5×10 −7 to 5×10 −3 mg/mL ( R 2 =0.998) was achieved in the presence of phoxim. Such simple and accurate LMOFs- FRET-based sensing strategy could offer valuable reference for developing sensitive FRET sensors and/or on-site evaluation approaches in the areas of food quality control and food safety.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Sensor Letters
·
2014
·Vol. 12
·期 10
·1462-1468
·
IF 0.558
Liu Zhi-Min,Zheng Ying-Ying,Jing Yan-Feng,Li Jie,Zhan Hai-Jun,Shen Qi
A facile, one-step synthesis of ionic liquid-functionalized graphene (IL-GR) nanocomposite using graphene oxide (GO) and amine-terminated ionic liquid (NH2-IL) was first presented through an epoxide ring-opening reaction, then acetylcholinesterase (AChE) was immobilized onto
IL-GR nanocomposite film modified glassy carbon electrode to prepare a highly sensitive AChE biosensor. The morphologies and electrochemistry properties of the composite were investigated by using scanning electron microscopy, transmission electron microscopy and cyclic voltammetry, respectively.
The nanocomposite not only provided a biocompatible microenvironment to keep the bioactivity of AChE, but also exhibited a strong synergetic effect on improving the sensing properties of phoxim. The immobilized AChE showed favorable affinity to acetylthiocholine (ATCl) and could catalyze the
hydrolysis of ATCl with a Kapp
m
value of 0.143 mM. The proposed biosensor exhibited a detection limit of 6.0 × 10–11 M (S/N = 3) towards phoxim. Besides, the biosensor is easy to prepare, have good stability, and will have potential application
in organophosphate pesticides.
研究领域:
分析化学
·
环境科学
·
生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）