ANALYTICAL CHEMISTRY
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2022
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IF 8.008
Yamin Ma,Cheng Liu,Lishi Wang
A strategy for the fast analysis of ion transfer/facilitated ion transfer toward a tiny (femtoliter) water-in-oil droplet has been established. This scenario is embodied by the fusion of a w/o microdroplet at the micro liquid/liquid (L/L) interface, with the use of Fourier transform fast-scan cyclic voltammetry (FT-FSCV) to express the apparent half-wave potentials of anions or cations encapsulated inside the w/o microdroplet. First, the half-wave potential is in strict accordance with the transfer Gibbs free energy of either cations or anions. Second, the half-wave potential has been found to be positively proportional to the logarithmic concentration of ions, shedding thermodynamic insight into ion transfer. Third, as an instance of multivalent biopolymers, the transfer of protamine inside the single w/o microdroplet has been investigated. Obvious discrepancies in the behaviors of the fusion impacts at different pH, as well as in the absence and presence of the cationic surfactant DNNS–, are revealed. The internal mechanism of protamine transfer has been thoroughly investigated. This work proposes a strategy to sensitively and quickly determine the transfer Gibbs energy and the concentration of ions encapsulated in a single microdroplet, and it provides the possibility of analyzing the interfacial transfer properties of trace biomacromolecules inside an aqueous micro- or nanoscale droplet.
研究领域:
分析化学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yun Liu,Guoqiang Zhang,Jia Chen,Ziying Zhang,Quanxin Wu,Lei Zhang,Shanhui Zhu,Qikai Liang,Zhongtao Wu,Xiliang Luo,Zhen Wang,Weishen Zeng
Protamine (PA) is the only licensed antidote for reversing heparin anticoagulation by electrostatically binding with heparin. Efforts have been made on designing various heparin-scavengers, while, it remains a great challenge for gaining the external-stimuli responsive PA-release material. In this study, a generic strategy is developed for fabricating photoresponsive protein materials with the designed azobenzene-containing surfactant. For the first time, based on the isomerization of azobenzene, both cationic and anionic proteins could be phase change biomaterials which are capable of transiting to isotropic state under UV irradiation at room temperature. The formation of isotropic state could set the proteins free from the binding state, activating their intrinsic biological functions. Employing this mechanism, one smart PA material for inhibiting heparin is developed, which could effectively photo-modulate the heparin concentration by turning on-and-off the free state of PA from the binding state. With good biocompatibility, the PA material addresses photoresponsive hemostatic activity in biological studies, confirming its great potential clinical values. This work provides a new designing strategy for gaining photocontrollable hemostasis materials, also opening new opportunities for developing photoresponsive protein drugs and biomedical materials.
研究领域:
生物医学工程
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材料科学
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药物开发
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 4
·1-7
·
IF 6.232
Huang Qingwei,Zhang Jinli,Li Wei,Fu Yan
Heparin was employed as the stabilizing agent in the synthesis of peroxidase-mimicking Pd nanoparticles. The heparin-capped Pd nanozyme can act as both the signal amplifier and the selective binder of protamine. The most efficient nanozyme with the mean size of 3.5 nm consists of 70.8% metallic Pd 0 and 29.2% Pd 2+ species. Enzyme kinetic studies show that the K m values are 0.036 mM for 3,3′,5,5′-tetramethylbenzidine and 78 mM for H 2 O 2 . Protamine shows strong affinity to the heparin-capped Pd nanozyme, and induces an apparent aggregation of the nanoparticles. This results in a significant inhibition of the peroxidase-mimicking activities. Hence, the oxidation of TMB by H 2 O 2 to a blue product with a maximum absorption at 652 nm is suppressed. Based on this finding, a photometric assay is developed for the determination of protamine. The linear response is in the concentration range 0.02 ~ 0.8 μg mL −1 , and the limit of detection is 0.014 μg mL −1 . This assay presents high selectivity toward other biological substances.
Highly active and selective Pd nanozyme was synthesized through adopting heparin as the capping agent for quantitative determination of protamine.
研究领域:
分析化学
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纳米技术
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生物化学
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DOI
PubMed
高影响因子
Acta Pharmaceutica Sinica B
·
2017
·
IF 0
Zhili Yu,Junxiao Ye,Xing Pei,Lu Sun,Ergang Liu,Jianxin Wang,Yongzhuo Huang,Seung Jin Lee,Huining He
RNAi technology has aroused wide public interest due to its high efficiency and specificity to treat multiple types of diseases. However, the effective delivery of siRNA remains a challenge due to its large molecular weight and strong anionic charge. Considering their remarkable functions in vivo and features that are often desired in drug delivery carriers, biomimetic systems for siRNA delivery become an effective and promising strategy. Based on this, covalent attachment of synthetic cell penetrating peptides (CPP) to siRNA has become of great interest. We developed a monomeric covalent conjugate of low molecular weight protamine (LMWP, a well-established CPP) and siRNA via a cytosol-cleavable disulfide linkage using PEG as a crosslinker. Results showed that the conjugates didn't generate coagulation, and exhibited much better RNAi potency and intracellular delivery compared with the conventional charge-complexed CPP/siRNA aggregates. Three different synthetic and purification methods were compared in order to optimize synthesis efficiency and product yield. The methodology using hetero-bifunctional NHS–PEG–OPSS as a crosslinker to synthesize LMWP–siRNA simplified the synthesis and purification process and produced the highest yield. These results pave the way towards siRNA biomimetic delivery and future clinical translation.
研究领域:
药学
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分子生物学
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药物递送系统
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DOI
PubMed
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Yuxia Gao,Kailun Wei,Jianzuo Li,Ying Li,Jun Hu
In this research a four-armed amino-tailored tetraphenylethene TPE-NH 2 was synthesized, and it could detect heparin (Hep) quantitatively, primarily promoted by the electrostatic interactions between its positively charged amino groups and the sulfates, carboxylates of heparin. On account of the aggregation-induced emission (AIE) nature of tetraphenylethene backbone, the aggregation restricted the rotation of C C single bond through rigidifying the conformation of TPE-NH 2 , thus making it display intense fluorescence with a broad working range (≤2.0 μg/mL) and a low detection limit (down to 35.89 ng/mL) for heparin. Meanwhile, the TPE-NH 2 /Hep complex can be further disaggregated by protamine due to the stronger affinity between heparin and protamine, consequently leading to the highly sensitive detection of protamine. What’s more, the low toxicity of TPE-NH 2 makes it a potential functional sensor for detecting heparin in the matrix of human serum.
研究领域:
生物化学
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医学检测
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材料科学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
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2022
·
IF 7.425
Wanwen Chen,Xiaojiao Li,Hao Cheng,Xiaobei Zhan,Wenshui Xia
Selenium nanoparticles (SeNPs) have attracted much recent interest as nutraceuticals, while they face great challenges, such as poor stability and low cellular uptake efficiency. This study introduced a facile approach to synthesizing protamine sulfate (PS) functionalized selenium nanoparticles (PS-SeNPs) by using PS as a surface decorator. The monodisperse spherical PS-SeNPs with a particle size of 130 nm and a ζ-potential of +31 mV were ligated with PS through Se-N, Se-O bonds, and physical adsorption, which exhibits excellent physical stability against pH, temperature, and storage time. The positive surface charge of PS-SeNPs contributed to the enhancement of cellular uptake efficiency by endocytosis, which was 3-times higher than bare SeNPs. Compared to SeNPs (IC 50 = 17.675 μg/mL), PS-SeNPs could dramatically inhibit the proliferation of HepG2 cells with an IC 50 value of 5.507 μg/mL, as reflected by the induction of apoptosis, S phase arresting, overproduction of intracellular ROS, and depolarization of mitochondria membrane. Overall, these results demonstrated the great potential of PS-SeNPs that can be applied as a functional ingredient in foods and nutraceuticals.
研究领域:
纳米医学
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药理学
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生物化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Maoshuang Chen,Jianan ShangGuan,Junyi Jiang,Jingjing Jiang,Fengjuan Li,Qingqi Dong,Hongyan Diao,Xiangdong Liu
The development of antibacterial cotton fabrics with an overall performance is critical but remains challenging. In this study, we propose a facile method to prepare durable antibacterial cotton fabric without significant sacrifices of wearing comfortability. Cotton fabric is firstly oxidated to obtain dialdehyde groups, then treated with PM molecules to establish a PM coating on the fiber surfaces via Schiff base linkages. The resultant cotton fabrics show durably antibacterial activity, realizing high bacterial reduction rates against both E. coli and S. aureus higher than 99.99 %, and offering remarkable durabilities tolerable 50 washing cycles and 500 rubbing times. These fabrics also show reliable safety for human skin that proofed by a series of cytotoxicity tests with positive results. This work demonstrates an example of versatile strategy to impart effective antibacterial function with durable activity to cotton textiles, showing great potential for practical applications in functional textile fields.
研究领域:
纺织科学
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生物医学工程
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DOI
PubMed
高影响因子
TETRAHEDRON
·
2021
·
IF 2.457
Xuwei Zheng,Jianan Dai,Baoxing Shen,Xing Zhang
An aggregation induced emission (AIE) fluorescent protein chromophore derivative CIC-FP for detecting protamine and heparin under physiological environment was designed and synthesized. CIC-FP exhibited good biocompatibility and a large stoke shift (91 nm), thus well attenuating the background interference, which could be used for quantitative detection of heparin and protamine . Since protamine has strong positively groups, it can interact with CIC-FP where bearing carboxyl through electrostatic interactions to form a tightly bound CIC-FP/protamine complex, thus showing superior fluorescence intensity response with a detection limit of 65.28 ng/mL. Because heparin has a stronger affinity with protamine, it would compete with CIC-FP on protamine, resulting in the release of CIC-FP and making CIC-FP change into free state with decreased fluorescence intensity . Besides, CIC-FP was successfully applied to detect spiked protamine in human serum samples. In summary, CIC-FP can be used to sensitive detection of protamine with good accuracy, which provides a new idea for designing protamine probes.
研究领域:
生物化学
·
医学检测
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2018
·
IF 3.862
Liping Zhang,Yanan Shi,Yina Song,Xinfeng Sun,Xuemei Zhang,Kaoxiang Sun,Youxin Li
Although oral delivery of exenatide has significant advantages, its poor permeability through intestinal epithelial membranes and rapid digestion by pepsin and ereptase in the gastrointestinal tract make effective oral delivery of exenatide a formidable challenge. In this study, we constructed a zinc ion (Zn 2+ ) and exenatide complex functionalized nanoparticle (NP) oral delivery system to overcome the above-mentioned issue. Polyethylene glycol-poly(lactic-co-glycolic acid) (PEG-PLGA) was used as a drug carrier to escape enzymatic degradation in the gastrointestinal tract, and low molecular weight protamine (LMWP) was used as a functional group to increase penetration of NPs into the intestinal epithelium. The functionalized NPs exhibited significantly improved penetration across the intestinal epithelium, as shown by cell uptake and transmembrane transport experiments. Moreover, a significant hypoglycemic effect was observed in diabetic rats. The relative bioavailability of the orally administered functionalized NPs vs. subcutaneous injection was 7.44%, 29-fold that of the exenatide-Zn 2+ solution group. These findings indicate that our modification could effectively improve exenatide treatment.
研究领域:
药剂学
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生物医学工程
·
糖尿病治疗
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DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2024
·
IF 5.8
Hang Hu,Ting Yuan,Jiahao Qian,Xinya Lu,Xi Chen,Xiaoya Sun,Yuchen Lin,Yuxiang Tang,Qingbo Xu,Tianyu Zhu,Yu Hu,Defeng Xu
Photodynamic therapy attracts great attention in cancer treatment due to its minimal invasiveness, good tolerance and specific cancer targeting. However, most of tumors are hypoxic and the tumors will become more hypoxic after photodynamic therapy, which might cause the poor prognosis by inducing tumor angiogenesis. In the present work, polyethylene glycol modified protamine-chlorin e6 conjugate (PEG-protamine-Ce6) was synthesized for enhanced cancer photodynamic therapy. PEG-protamine-Ce6 was synthesized by conjugating protamine with methoxypolyethylene glycol acetaldehyde and Ce6 sequentially. The in vitro studies show that PEG-protamine-Ce6 self-assembles into nanoparticles in aqueous solution with uniform size (∼200 nm), positive zeta potential and spherical morphology. The in vitro cell studies show that PEG-protamine-Ce6 nanoparticles exhibit enhanced cellular uptake amount, intracellular ROS level elevation upon laser irradiation, cell apoptosis and photocytotoxicity as compared to Ce6. The in vivo studies show that PEG-protamine-Ce6 nanoparticles exhibit enhanced antitumor effect as compared to Ce6 upon laser irradiation. PEG-protamine-Ce6 with laser irradiation not only inhibits tumor cell proliferation, but also inhibits tumor angiogenesis by suppression of VEGF expression, whereas Ce6 with laser irradiation promotes tumor angiogenesis by enhancing VEGF expression. PEG-protamine-Ce6 nanoparticles developed in this work offer a novel nanomedicine for enhancing cancer photodynamic therapy.
研究领域:
药学
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肿瘤学
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纳米医学
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DOI
高影响因子
ChemistrySelect
·
2024
·Vol. 9
·期 9
·e202303466
·
IF 1.9
Xiaochen Gao,Ke Li,Yu Xiao,Jianan Dai,Zheyu Shen,Guangping Lv,Yaqiong Yang
Graphical In this research, a new TICT probe (IDL-FP) was designed for sensitive detection of protamine. The introduction of indole-3-carboxaldehyde and 4-dimethylaminobenzaldehyde gave IDL-FP both an electron-donor and electron-acceptor, which urged the complete intramolecular charge transfer and a large stokes shift, allowing IDL-FP to process minimal background noise and excellent biocompatibility. Additionally, protamine can directly induce the aggregation of IDL-FP caused by electrostatic attraction. In this way, the turn-on detection of protamine was achieved and the detection limit was as low as 8.87 ng mL −1 , which showed a better sensitivity and higher stability. As IDL-FP exhibited good stability in physiological pH environment, we successfully applied IDL-FP to detect spiked-in protamine in human serum. In summary, probe IDL-FP is a promising tool for the quantitative determination of protamine for potential clinical usage. As effective methods to accurately and rapidly detect protamine remains a major challenge, here we present a fluorescent protein chromophore-based probe (IDL-FP) utilizing twisted-intramolecular charge transfer (TICT) to detect protamine. The introduction of an electron-donor group 4-dimethylaminobenzaldehyde extends the emission wavelength of IDL-FP to the red region. The deprotection of t-butyloxy carbonyl groups can then generate an electronegative carboxyl group, which promotes the binding to protamine via electrostatic attraction. Thus, IDL-FP is sensitive and specific towards protamine and can serve as a highly selective detector for protamine with a detection limit as low as 8.87 ng/mL. As IDL-FP exhibited good stability in physiological pH environment, we successfully apply IDL-FP to detect spiked-in protamine in human serum. In summary, probe IDL-FP is a promising tool for the quantitative determination of protamine.
研究领域:
生物化学
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医学检测
·
分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 4
·1-8
·
IF 5.7
Wu Ning-Ning,Chen Lin-Ge,Xiao Min-Zhi,Yuan Rong-Yao,Wang Hai-Bo
A fluorescent method is described for trypsin determination through the strong electrostatic interactions between cationic polyelectrolytes and single-stranded DNA (ssDNA) templated Au nanoclusters (AuNCs). The ssDNA-AuNCs display improved fluorescence emission with excitation/emission maxima at 280/475 nm after being incorporated with poly(diallyldimethylammonium chloride) (PDDA). Fluorescent enhancement is mainly attributed to the electrostatic interactions occurring between PDDA and ssDNA templates. This can make the conformation of the ssDNA templates to change. Thus, it offers a better microenvironment for stabilizing and protecting ssDNA-AuNCs, and results in fluorescence emission enhancement. By using protamine as a model, the method is employed for the determination of trypsin. The assay enables trypsin to be determined with good sensitivity and a linear response ranging from 5 ng⋅mL −1 to 60 ng⋅mL −1 with a 1.5 ng⋅mL −1 limit of detection. It is also extended to determine the trypsin contents in human’s serum samples with recoveries between 98.7% and 103.5% with relative standard deviations (RSDs) between 3.5% and 4.8%. Graphical abstract A novel fluorescent strategy has been developed for of trypsin determination by using protamine mediated fluorescent enhancement of DNA templated Au nanoclusters.
研究领域:
分析化学
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生物化学
·
纳米技术
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）