FOOD CHEMISTRY
·
2023
·
IF 8.8
Beibei Zhao,Xinfang Liu,Zheng Cheng,Xu Liu,Xiaoyu Zhang,Xun Feng
Pymetrozine (PYM) is an effective pyridine insecticide for controlling aphids, while its residues pose a serious threat to human health. Herein, a europium complex (Eu-DBPA, DBPA represents deprotonated 2,5-dibromoterephthalic acid ligand) probe was prepared for the detection of PYM via fluorescence quenching . The detection process has the advantages of short response time (2 min), wide linear range (0–4 and 4–45 mg/kg) and low detection limit (2.2 μg/kg). Furthermore, a portable detection platform was designed by integrating Eu-DBPA-based paper strip with smartphone and applied for the visual detection of PYM in real cucumber, tomato, cabbage and apple samples, obtaining satisfactory recovery (99.00 %–107.00 %) and low standard deviation (RSD < 3.4 %). In addition, a logic gate device was designed to simplify the detection process. The smartphone-integrated paper-based probe detection platform provides a new strategy for intelligent and online identification of hazards in environmental and biological samples.
研究领域:
分析化学
·
环境科学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 77
·期 9
·4128-4137
·
IF 4.845
Li-Xiang Wang,Sha Tao,Yan Zhang,Ya-Long Jia,Shun-Fan Wu,Cong-Fen Gao
BACKGROUND Pymetrozine is commonly used for the control of Nilaparvata lugens , and resistance to pymetrozine has been frequently reported in the field populations in recent years. However, the mechanism of brown planthopper resistance to pymetrozine is still unknown. RESULTS In this study, a pymetrozine-resistant strain (PMR) was established, and the potential biochemical resistance mechanism of N. lugens to pymetrozine was investigated. Pymetrozine was synergized by the inhibitor piperonyl butoxide (PBO) in the PMR with 2.83-fold relative synergistic ratios compared with the susceptible strain (Sus). Compared with the Sus, the cytochrome P450 monooxygenase activity of PMR was increased by 1.7 times, and two P450 genes ( NlCYP6CS1 and NlCYP301B1 ) were found to be significantly overexpressed more than 6.0-fold in the PMR. Pymetrozine exposure induced upregulation of NlCYP6CS1 expression in the Sus, but the expression of NlCYP301B1 did not change significantly. In addition, RNA interference (RNAi)-mediated suppression of NlCYP6CS1 gene expression dramatically increased the toxicity of pymetrozine against N. lugens . Moreover, transgenic lines of Drosophila melanogaster expressing NlCYP6CS1 were less susceptible to pymetrozine, and had a stronger ability to metabolize pymetrozine. CONCLUSIONS Taken together, our findings indicate that the overexpression of NlCYP6CS1 is one of the key factors contributing to pymetrozine resistance in N. lugens . And this result is helpful in proposing a management strategy for pymetrozine resistance.
研究领域:
昆虫学
·
分子生物学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2022
·
IF 6.443
Meiting Guo,Fang Wang,Wang Guo,Run Tian,Tingtiao Pan,Ping Lu
Pymetrozine is widely used in agriculture to control pests, and its residue may pose a threat to humans. In this study, the adsorption behavior of pymetrozine on Au@AgNPs surfaces in different solutions was investigated by calculation of ACD/Labs, density functional theory, UV–vis spectra, zeta potential and surface-enhanced Raman scattering. Then, a SERS method for detection of pymetrozine residues in apples was established based on the adsorption study. The results showed that pymetrozine was adsorbed on Au@AgNPs surface in different forms in various solutions and high SERS sensitivity of pymetrozine was obtained by the synergistic effect of pymetrozine, Au@AgNPs and NaOH. A simple SERS method has been established to detect pymetrozine in apples with a LOD of 0.038 mg/kg, linear range of 0.05–1.00 mg/kg, recovery of 71.93–117.49 % and RSD low than 11.70 %. This study provides a reference for rapid detection of pymetrozine in agricultural products.
研究领域:
分析化学
·
食品安全
·
纳米材料
·
农药残留检测
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表面增强拉曼光谱技术
✓ 已验证
DOI
高影响因子
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
·
2025
·
IF 4
Tengfei Liu,Jianguo Niu,Yuying Gao,Xuan Liu,Jianya Su
Flonicamid is an insecticide with a unique mode of action, primarily inhibiting the feeding behavior of sap-sucking insects. However, its molecular target remains controversial. Reports indicate that both flonicamid and pymetrozine affect insect behavior and mobility. To investigate further, we compared the susceptibility of three insect species ( Myzus persicae , Nilaparvata lugens , and Drosophila melanogaster ) to flonicamid and pymetrozine under two different feeding postures. Results showed that feeding posture did not affect the survival curves or mean lifespans of the three insects. However, insect lifespans were significantly reduced under exposure to either insecticide compared to untreated controls. The effects of the two insecticides on susceptibility and lifespan under different feeding postures were markedly different: feeding posture significantly influenced the sensitivity of aphids, planthoppers, and fruit flies to pymetrozine but had minimal effects on the sensitivity of aphids and planthoppers to flonicamid. Flonicamid had only a minor impact on fruit flies' sensitivity. Survival curve analysis also revealed significant differences under pymetrozine stress, while no such differences were observed under flonicamid stress. These findings indicate that flonicamid exerts different toxicological effects on target insects (aphids and planthoppers) versus non-target insects (fruit flies), depending on feeding posture. This suggests that the toxicological mechanisms of flonicamid differ substantially between target and non-target organisms, with implications that findings in non-target insects may not directly apply to target pests. Our study provides valuable insights and directions for further research into flonicamid's mode of action, aiding in elucidating its underlying mechanisms.
研究领域:
昆虫学
·
毒理学
·
农药科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2023
·
IF 3.8
Pei-Hui Shan,Ding-Wu Pan,Li-Xia Chen,Timothy J. Prior,Carl Redshaw,Zhu Tao,Xin Xiao
Herein, we report a simple fluorescence-enhanced system for the selective recognition and determination of the insecticide pymetrozine. 1 H NMR spectroscopic data indicate that 1,2-bis(4-pyridyl)ethylene (BPE) is partially encapsulated in the cavity of the cucurbit[8]uril (Q[8]) in aqueous solution, forming a stable 1:2 host-guest inclusion complex. Good evidence is also provided by other characterization techniques including single crystal X-ray diffraction, UV–Vis and fluorescence spectroscopies. This host-guest inclusion complex shows weak fluorescence in aqueous solution. Interestingly, the addition of pymetrozine greatly enhanced the fluorescence of the host-guest inclusion complex. In contrast, no significant fluorescence enhancement was observed on addition of 10 other pesticides. The concentration of pymetrozine in aqueous solution was easily detected based on the linear relationship between fluorescence intensity and pymetrozine concentration. Therefore, this paper reports a new method to identify and determine pymetrozine by fluorescence enhancement.
研究领域:
分析化学
·
农药科学
✓ 已验证
DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2020
·Vol. 138
·期 11
·50040
·
IF 2.52
Shupeng Zhang,Lei Zhang,Xiaodong Yang,Wendong Hua,Junyun Qiu,Mingqian Zhang,Bo Zhang,Tianrui Ren
Coalescence and flocculation are two challenges for the stability and efficacy of pesticide suspension concentrate (SC) during the production and storage. To improve the stability of 25% pymetrozine SC, three comb-like poly(styrene-co-acrylic acid-co-polyethylene glycol methyl ether maleate) (SA-M) with different length side chains were synthesized by free radical polymerization and characterized by Fourier transform infrared spectroscopy and H-nuclear magnetic resonance. Surface-active parameters, including critical aggregation concentration (critical micelle concentration), saturation surface excess concentration ( Γ max ), minimum area per molecule ( A min ), and standard Gibbs free energy values were obtained from surface tension measurement. It shows that the surface-active property of SA-M decreased with the side chain length. Besides, 25% pymetrozine SC prepared using SA-M as dispersant had low zeta potential and low viscosity but high dispersibility and thermal storage stability. It is speculated that the formation of hydrogen bonds between nonionic branches and pymetrozine molecule at the expense of interruption of intermolecular hydrogen bonding between pymetrozine and water molecule, plays a key role in the stability mechanism.
研究领域:
农药科学
·
高分子化学
·
表面化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 10
·期 4
·417-421
·
IF 1.9
Jing-yan Kang,Yu-jie Zhang,Xing Li,Chen Dong,Hong-yu Liu,Li-jing Miao,Paul J. Low,Zhi-xian Gao,Narayan S. Hosmane,Ai-guo Wu
A colorimetric sensor for the pesticide pymetrozine (PYM) based on analyte-induced aggregation of melamine-modified gold nanoparticles (M-Au NPs) has been developed with high selectivity and sensitivity with a detection limit of 80 nM by the naked eye and 10 nM by UV-Vis spectroscopy. This colorimetric sensing design exhibits lower detection limits, compared with limits of 46–148 nM for existing instrumental methods. The new aggregation-induced nanoparticle sensor is applicable for rapid colorimetric detection of PYM in real water environments (tap water and lake water) and food samples (green tea and apple juice). The technical approach for the colorimetric sensor is easier and simpler than detection of PYM by, for example, HPLC whilst giving equivalent results within the error of the measurements.
研究领域:
环境科学
·
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2021
·
IF 10.588
Ximei Liang,Fangling Guan,Zhiyou Ling,Honghong Wang,Yunwen Tao,Elfi Kraka,Huajun Huang,Chenglong Yu,Danping Li,Jinbao He,Hansun Fang
Photodegradation of the insecticide pymetrozine (PYM) was studied on surface of wax films, and in aqueous and nonaqueous phase. The half-life of PYM on the wax surface was approximately 250 times longer than in water. Scavenging experiments, laser flash photolysis, and spectra analysis indicated the first singlet excited state of PYM (S1 * PYM ) to be the most important photoinduced species initiating the photodegradation. Quantum chemistry calculations identified significant molecular torsion and changes in the structure C–C N–N of S1 * PYM , and the absolute charges of the C N atoms increased and the bond strength weakened. Free energy surface analysis, and O 18 labeling experiments further confirmed that the mechanism was two-step photoinduced hydrolysis. The first step is the hydrolysis of S1 * PYM at C N upon reaction with 2–3 water molecules (one H 2 O molecule as the catalyst). The second step is an intramolecular hydrogen transfer coupled with the cleavage of C–N bond and formation of two cyclic products. During the interactions, water molecules experience catalytic activation by transferring protons, while there is a negligible solvent effect. Clarifying the detailed photodegradation mechanisms of PYM is beneficial for the development of green pesticides that are photostable and effective on leaf surfaces, and photolabile and detoxified in the aquatic environment.
研究领域:
环境科学
·
农药化学
·
量子化学
✓ 已验证
DOI
高影响因子
Analytical Methods
·
2015
·Vol. 7
·期 21
·9100-9107
·
IF 1.821
Donglin Jia,Jian Gao,Lu Wang,Yudong Gao,Baoxian Ye
A simple and sensitive electrochemical method for the determination of the insecticide pymetrozine was proposed using a simple electrochemically pretreated glassy carbon electrode (EPGCE). Compared with the bare GCE, the electrochemical response signal of pymetrozine at the EPGCE showed a significant increase. The electrochemical behavior of pymetrozine was investigated systematically and some dynamics were investigated in detail for the first time. The results indicate that the reaction of pymetrozine on the EPGCE is a two electron and two proton process, which is controlled by both diffusion and adsorption. Under optimum conditions, a good linear relationship was obtained between peak currents and pymetrozine concentrations in the range of 1 × 10−7 to 5 × 10−6 mol L−1 with a detection limit of 8 × 10−8 mol L−1 (S/N = 3). The proposed method was applied to the determination of pymetrozine in real samples with satisfactory recovery results. Finally, the degradation of pymetrozine was also studied in natural soil.
研究领域:
分析化学
·
环境科学
·
农药检测
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Physiology
·
2022
·
IF 4.755
Zhao Ruoying,Li Danting,Wang Xinlong,Li Zhong,Yu Xiaoping,Shentu Xuping
Management of the rice brown planthopper Nilaparvata lugens Stål is challenging because it can rapidly adapt to new pesticides within several generations. Combined use of chemical insecticides and antimicrobials was proposed as an alternative strategy to control N. lugens. Our previous experiments identified two effective agents (chemical insecticide: pymetrozine and antimicrobial: zhongshengmycin) that act on different targets in N. lugens. However, conditions and effectiveness of combinations of antimicrobials and insecticides against N. lugens are still unknown. Here, we evaluated separate and combined effects of pymetrozine and zhongshengmycin on third instar nymphs of N. lugens under laboratory and greenhouse conditions. Results showed that zhongshengmycin exerts significant inhibitory effects on the three endosymbionts Pichia guilliermondii, Cryptococcus peneaus, and Pichia anomala cultured in vitro of N. lugens. Combinations of pymetrozine and zhongshengmycin under laboratory conditions produced additive or synergistic effects on N. lugens and caused higher mortality in third instar nymphs than either of them used alone. Experiments under greenhouse conditions further demonstrated that effective component quality ratio of pymetrozine to zhongshengmycin of 1:10 and 1:40 with co-toxicity coefficients of 221.63 and 672.87, respectively, also produced significant synergistic effects against N. lugens. Our results indicated that chemical insecticides combined with antimicrobials may provide a potential novel strategy for controlling N. lugens by inhibiting its endosymbionts.
研究领域:
昆虫学
·
植物保护
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Chemistry
·
2020
·Vol. 17
·期 6
·436-444
·
IF 1.91
Hansun Fang,Zhiyou Ling,Fangling Guan,Wei Liao,Faying Lai,Ximei Liang
Environmental context Photodegradation, a natural process for removing pesticides from water, is particularly important in rice fields with their large surface area and sunlight exposure. By studying two commonly used pesticides, we show that the properties of rice field water, such as dissolved organic matter, facilitate indirect photolytic processes while reducing direct photolysis. A better understanding of the natural degradation mechanisms for pesticides will inform their safer use in agriculture. Rice paddy fields (RFs) make ecologically important contributions to agricultural wetland ecosystems in south China. In recent years, the elevated use of pesticides has resulted in concerns over the water quality in RFs and their downstream aquatic environment. Photodegradation is important for the natural elimination of nonpoint source agricultural contaminants, and RF water provides an ideal environment for the photochemical removal of pesticides, owing to its large surface area and photic conditions. Therefore, in this study, the physical and photochemical properties of water collected from the irrigation area were investigated, along with the direct and indirect photodegradation of two commonly used pesticides in RFs, tricyclazole (TC) and pymetrozine (PYM). Results indicated that allochthonous-like dissolved organic matter (DOM) was abundant in the irrigation water, possibly originating from the decomposition of rice straw. Moreover, the light screen effect and the formation rate of singlet oxygen (1O2) and the triplet excited state of DOM (3DOM*) were generally increased in RF water, while the formation of HO• was marginally reduced. Evapoconcentration and photobleaching processes were found to correlate with crop leaf coverage and had a significant impact on the levels and compositions of water constituents. Finally, the photodegradation kinetics of TC and PYM further confirmed that RF water would facilitate indirect photolysis and reduce the direct photolysis of pesticides.
研究领域:
环境科学
·
农业生态学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 8
·1874
·
IF 4.35
Ting-Tiao Pan,Mei-Ting Guo,Wang Guo,Ping Lu,De-Yu Hu
Residual pesticides are one of the major food safety concerns around the world. There is a demand for simple and reliable methods to monitor pesticide residues in foods. In this study, a sensitive method for determination of pymetrozine in apple and cabbage samples using surface-enhanced Raman spectroscopy (SERS) based on decanethiol functionalized silver nanoparticles was established. The proposed method performed satisfactorily with the linear detection range of 0.01–1.00 mg/L and limit of detection (LOD) of 0.01 mg/L in methanol. In addition, it was successfully used to detect pymetrozine in apple and cabbage samples, the LOD was 0.02 and 0.03 mg/L, respectively, and the recoveries of spiked cabbage and apple ranged 70.40–104.00%, with relative standard deviations below 12.18% and 10.33% for intra-day and inter-day tests. Moreover, the results of the correlation test with real cabbage samples of liquid chromatography-tandem mass spectrometry showed that they were highly correlated (slope = 0.9895,R2= 0.9953). This study provides a sensitive approach for detection of pymetrozine in apple and cabbage, which has great potential for determination of pymetrozine residues in food products.Keywords:insecticide;pymetrozine;residual detection;food;surface-enhanced Raman spectroscopy
研究领域:
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）