International Journal of Nanomedicine
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2023
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IF 8
Yuanjing Zhu,Fangfang Song,Yanyun Ju,Liyuan Huang,Lu Zhang,Chuliang Tang,Hongye Yang,Cui Huang
Purpose In this study, we aimed to develop a unique N-acetyl cysteine (NAC)-loaded polylactic-co-glycolic acid (PLGA) electrospun system with separate compartments for the promotion of osteogenesis.Materials and methods We first prepared solutions of NAC-loaded mesoporous silica nanoparticles (MSNs), PLGA, and NAC in N, N-dimethylformamide and tetrahydrofuran for the construction of the electrospun system. We then fed solutions to a specific injector for electrospinning. The physical and chemical properties of the scaffold were characterized through scanning electron microscopy, transmission electron microscopy, and Fourier transform infrared spectroscopy. The release of NAC and Si from different PLGA scaffolds was estimated. The cell viability, cell growth, and osteogenic potential of rat bone marrow-derived stroma cell (rBMSCs) on different PLGA scaffolds were evaluated through MTT assay, live/dead staining, phalloidin staining, and Alizarin red staining. The expression levels of osteogenic-related markers were analyzed through real-time PCR (qRT-PCR).Results NAC was successfully loaded into MSNs. The addition of MSNs and NAC decreased the diameters of the electrospun fibers, increased the hydrophilicity and mechanical property of the PLGA scaffold. The release kinetic curve indicated that NAC was released from (PLGA + NAC)/(NAC@MSN) in a biphasic pattern, that featured an initial burst release stage and a later sustained release stage. This release pattern of NAC encapsulated on the (PLGA + NAC)/(NAC@MSN) scaffolds enabled to prolong the high concentrations of release of NAC, thus drastically affecting the osteogenic differentiation of rBMSCs.Conclusion A PLGA electrospun scaffold was developed, and MSNs were used as separate nanocarriers for recharging NAC concentration, demonstrating the promising use of (PLGA + NAC)/(NAC@MSN) for bone tissue engineering.
研究领域:
组织工程
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材料科学
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细胞生物学
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再生医学
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
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2024
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IF 6.1
Xu Wei,Chang Wan,Xu Peng,Yihao Luo,Mengyue Hu,Can Cheng,Shaoxiong Feng,Xixun Yu
Biomaterials with dual functions of osteoimmunomodulation and bone repair are very promising in the field of orthopedic materials. For this purpose, we prepared a copper-based carbon dots (CuCDs) and doped it into oxychondroitin sulfate / poly-acrylamide hydrogel (OPAM) to obtain a hybrid hydrogel (CuCDs/OPAM). We evaluated its osteoimmunomodulatory and bone repair properties in vitro and in vivo. The obtained CuCDs/OPAM exhibited good rBMSCs-cytocompatibility and anti-inflammatory properties in vitro. It also could effectively promote rBMSCs differentiation and the expression of osteogenic differentiation factor from rBMSCs under an inflammatory environment. Moreover, CuCDs/OPAM could induce macrophage phenotype switching (from M1-type macrophages to M2-type macrophages) in vivo which was beneficial to anti-inflammatory and present a good osteoimmunomodulation capability to induce a bone immune microenvironment to promote the differentiation of rBMSCs. In conclusion, CuCDs/OPAM hydrogel has dual functions of osteoimmunomodulatory and bone repair, and is a promising bone filling and repair material.
研究领域:
生物材料
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骨科
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Stem Cell Research & Therapy
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2020
·Vol. 11
·期 1
·1-13
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IF 5.116
Chai Miaomiao,Gu Ce,Shen Qihua,Liu Jiaxing,Zhou Yi,Jin Ziyang,Xiong Wanli,Zhou Yan,Tan Wensong
Background and aim Inadequate vascularization is a challenge in bone tissue engineering because internal cells are prone to necrosis due to a lack of nutrient supply. Rat bone marrow-derived mesenchymal stem cells (rBMSCs) and human umbilical vein endothelial cells (HUVECs) were cocultured to construct prevascularized bone tissue in osteogenic induction medium (OIM) in vitro. The angiogenic capacity of HUVECs was limited in the coculture system. In this study, the effects of the components in the medium on HUVEC angiogenesis were analyzed. Methods The coculture system was established in OIM. Alizarin red staining and alkaline phosphatase staining were used to assess the osteogenic ability of MSCs. A Matrigel tube assay was used to assess the angiogenic ability of HUVECs in vitro. The proliferation of HUVECs was evaluated by cell counting and CCK-8 assays, and migration was evaluated by the streaked plate assay. The expression levels of angiogenesis-associated genes and proteins in HUVECs were measured by qRT-PCR and Western blotting, respectively. Results Dexamethasone in the OIM suppressed the proliferation and migration of HUVECs, inhibiting the formation of capillary-like structures. Our research showed that dexamethasone stimulated HUVECs to secrete tissue inhibitor of metalloproteinase (TIMP-3), which competed with vascular endothelial growth factor (VEGF-A) to bind to vascular endothelial growth factor receptor 2 (VEGFR2, KDR). This effect was related to inhibiting the phosphorylation of ERK and AKT, which are two downstream targets of KDR. However, under hypoxia, the enhanced expression of hypoxia-inducible factor-1α (HIF-1α) decreased the expression of TIMP-3 and promoted the phosphorylation of KDR, improving HUVEC angiogenesis in the coculture system. Conclusion Coculture of hypoxia-preconditioned HUVECs and MSCs showed robust angiogenesis and osteogenesis in OIM, which has important implications for prevascularization in bone tissue engineering in the future.
研究领域:
组织工程
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细胞生物学
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分子生物学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2019
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IF 6.864
Syed Asadullah,Han Wu,Shiqi Mei,Deqiang Wang,Yongkang Pan,Dongliang Wang,Jun Zhao,Jie Wei
Polyimide (PI) may become a promising biomaterial for dental and orthopedic application due to its high mechanical strength , good biocompatibility and bio-stability as well as modulus of elasticity similar to the bone of human body. In this study, bioactive tantalum pentoxide (Ta 2 O 5 , TO)/polyimide (PI) composites (TPC) were fabricated by blending modification through a process of cold-pressing and sintering. The results revealed that the compressive strength , thermal stability, surface roughness , hydrophilicity and surface energy of TPC were significantly enhanced with the increase of TO content. Moreover, apatite mineralization of TPC in simulated body fluid (SBF) was obviously improved, in which TPC with TO of 50 wt% content (TPC50) displayed the best bioactivity . Furthermore, the cellular responses (adhesion, proliferation and differentiation) of rat bone mesenchymal stem cells (rBMSCs) to TPC were obviously upregulated with the increase of TO content. The results demonstrated that the TO content in the composites remarkably affected not only physical-chemical properties but also the cells behaviors. It can be suggested that TPC50 with improved surface properties and bioactivity as well as good cytocompatibility would have great potential and prospect for dental and orthopedic applications.
研究领域:
生物材料学
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骨科与牙科应用
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
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IF 3.973
Lishu Ren,Songchao Tang,Xuening Shen,Chao Gao,Yun Kyung Jung,Dongliang Wang,Axiang He,Lili Yang,Jung-Woog Shin,Jie Wei
Dental implanted materials require excellent mechanical properties, biocompatibility as well as integration with bone tissue and gingival tissue to achieve early loading and long-term stability. In this study, cubic shape sodium tantalite (ST) submicro-particles with the size of around 180 nm were synthesized by a hydrothermal method, and ST/polyetheretherketone (PEEK) composites (TPC) with ST content of 20 w% (TPC20) and 40 w% (TPC40) were prepared by melting blend. The results showed that the compressive strength, thermal properties, surface roughness, hydrophilicity and surface energy as well as adsorption of proteins on TPC40 were also significantly enhanced compared with TPC20 and PEEK. Moreover, the responses (adhesion and proliferation as well as differentiation) of rat bone marrow mesenchymal stem cells (rBMSCs), and responses (adhesion, and proliferation) of human gingival epithelial (HGE-1) cells to TPC40 were significantly promoted compared with TPC20 and PEEK. The results demonstrated that ST content in TPC had remarkable effects on the surface properties, which played key roles in stimulating the responses of both rBMSCs and HGE-1 cells. TPC40 with increased surface properties and excellent cytocompatibility might have great potential as an implanted material for dental application.
研究领域:
生物材料学
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口腔医学
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Today
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2021
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IF 20.722
Yang Liu,Xiaoping Zhang,Tengling Wu,Bo Liu,Jianhai Yang,Wenguang Liu
Injectable hydrogels and stem cells have been increasingly applied to treat myocardial infarction (MI). However, the harsh microenvironment adversely affects the survival of implanted stem cells and ultimate therapeutic outcomes. Herein, we design and prepare rat bone marrow mesenchymal stem cells (rBMSCs)-embedded polyzwitterionic microgels ( [email protected] ) via in situ Michael addition reaction between multivinyl carboxybetaine macromonomer and thiol-ended polyethylene glycol mixed with rBMSCs in a microfluidic system. The [email protected] are then loaded into a tyramine-modified hyaluronic acid (HA-Tyr), into which Chinese herb puerarin (PUE) and horseradish peroxidase (HRP)/H 2 O 2 are added to catalyze inter-phenol crosslinks to form an injectable HA-Tyr-PUE hydrogel encapsulating [email protected] ( [email protected] @microgels). This [email protected] @microgels is injected into the infarct region of rats, where HA-Tyr-PUE hydrogel serves to favorably modulate the microenvironment by scavenging active oxygen and releasing oxygen, and rBMSCs remain stemness in highly hydrophilic microgels and exert a paracrine effect to facilitate anti-inflammation and angiogenesis . These concerted actions boost the highly efficient restoration of ventricular function and structure. The ROS-scavenging medicine-crosslinked biodegradable biomacromolecule hydrogel bearing stemness-maintained microgels can be expanded to diverse tissue engineering and regenerative medicine fields.
研究领域:
组织工程
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再生医学
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生物材料
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干细胞治疗
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心脏病学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Materials
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2022
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IF 3.985
Zhu Peijun,Lai Chunhua,Cheng Mingwei,He Yiheng,Xu Yan,Chen Jiahao,Zhou Zhengnan,Li Ping,Xu Shulan
A biomaterial-mediated immune response is a critical factor to determine the cell fate as well as the tissue-regenerative outcome. Although piezoelectric-membranes have attracted considerable interest in the field of guided bone regeneration thanks to their biomimetic electroactivity, the influence of their different surface-charge polarities on the immune-osteogenic microenvironment remains obscure. The present study aimed at investigating the interaction between piezoelectric poly (vinylidene fluoridetrifluoroethylene) [P (VDF-TrFE)] membranes with different surface polarities (negative or positive) and macrophage response, as well as their subsequent influence on osteogenesis from an immunomodulating perspective. Specifically, the morphology, wettability, crystal phase, piezoelectric performance, and surface potential of the synthetic P (VDF-TrFE) samples were systematically characterized. In addition, RAW 264.7 macrophages were seeded onto differently charged P (VDF-TrFE) surfaces, and the culture supernatants were used to supplement cultures of rat bone marrow mesenchymal stem cells (rBMSCs) on the corresponding P (VDF-TrFE) surfaces. Our results revealed that oppositely charged surfaces had different abilities in modulating the macrophage-immune-osteogenic microenvironment. Negatively charged P (VDF-TrFE), characterized by the highest macrophage elongation effect, induced a switch in the phenotype of macrophages from M0 (inactivated) to M2 (anti-inflammatory), thus promoting the osteogenic differentiation of rBMSCs by releasing anti-inflammatory cytokine IL-10. Interestingly, positively charged P (VDF-TrFE) possessed pro-inflammatory properties to induce an M1 (pro-inflammatory) macrophage-dominated reaction, without compromising the subsequent osteogenesis as expected. In conclusion, these findings highlighted the distinct modulatory effect of piezoelectric-P (VDF-TrFE) membranes on the macrophage phenotype, inflammatory reaction, and consequent immune-osteogenic microenvironment depending on their surface-charge polarity. This study provides significant insight into the design of effective immunoregulatory materials for the guided bone regeneration application.
研究领域:
生物材料
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组织工程
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免疫学
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再生医学
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骨科
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Bo Wang,Shuai Yuan,Wei Xin,Yi Chen,Qiwei Fu,Lexiang Li,Yang Jiao
Bone defect repair and tissue engineering is specifically challenging process because of the distinctive morphological and structural behaviours of natural bone with complex healing and biochemical mechanisms . In the present investigation, we designed dopamine adhesive chemistry-based fabrication of silk fibroin hydrogel (SFD) with incorporation of nano-hydroxyapatite (nHA)-graphene oxide (GO) hybrid nanofillers with well-arranged porous morphology immobilized with bone morphogenic protein-2 (BMP-2) for the effective in vitro rabbit bone marrow derived mesenchymal stem cells loading compatibility and in vivo new bone regrowth and collagen deposition ability. We have achieved bone-specific hydrogel scaffolds with upgraded structural features, mechanical properties and particularly promoted in vitro osteogenic differentiation and compatibility of rabbit bone marrow mesenchymal stem cells (rBMSCs). Structural and microscopic analyses established greater distributions of components and well-ordered and aligned porous structure of the hydrogel network. In vivo result of new bone regrowth was promisingly higher in the Bm@nHG-SFD hydrogel (85%) group as compared to the other treatment groups of nHG-SFD (77%) and nH-SFD (64%) hydrogel. Overall, we summarized that morphologically improved hydrogel material with immobilization of BMP-2 could be have more attentions for new generation bone regeneration therapies.
研究领域:
生物材料学
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组织工程学
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再生医学
✓ 已验证
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2020
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IF 10.652
Junkai Zeng,Yitong Wang,Zhenyu Sun,Haishuang Chang,Mi Cao,Jie Zhao,Kaili Lin,Youzhuan Xie
Surgical site infection (SSI) with instrumentation is an undesired and troublesome complication in orthopaedics. Surgical debridement is currently the main treatment option. However, the poor prognosis remains the major problem and over 40% of patients needed additional surgery to remove the implant. To eliminate SSI, antibacterial and biocompatible implants are urgently needed. Herein, a novel biocompatible polydopamine (PDA)-IR820-Daptomycin (DAP) coating with triple therapy functions was successfully fabricated to enhance the anti-infection ability and osteogenic property of titanium implants. In which, PDA served as a biocompatible binder to immobilize IR820 and DAP, and possessed photothermal and osteogenic properties. The releasing of DAP inhibited the growth of Staphylococcus aureus and damaged the bacterial membrane. Meanwhile, after 808 nm laser irradiation, the combination of hyperthermia generated by PDA and singlet oxygen ( 1 O 2 ) produced by IR820 eradicated the biofilm noninvasively. Furthermore, this coating successfully accelerated glutathione (GSH) oxidation and destroyed the bacterial membrane, reaching an anti-bacterial efficiency of 97.2% in vitro . Additionally, the coating significantly stimulated the proliferation and osteogenic differentiation of rat bone marrow stem cells (rBMSCs). In vivo, Ti-PDA-IR820-DAP reached an anti-bacterial efficiency of 97.9% in a S. aureus biofilm-infected rat model with 15 min of irradiation at 50 °C and drastically promoted the bone-implant osseointegration in a rat femur condyle defect model. The present study demonstrates that combining antibiotics and phototherapy represents a feasible way to treat bone-implant-associated infections.
研究领域:
生物医学工程
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材料科学
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传染病学
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骨科
✓ 已验证
DOI
高影响因子
MATERIALS & DESIGN
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2022
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IF 9.417
Hanbang Chen,Jia Yan,Shuying Hu,Shangwen Sun,Fang Zhou,Jun Liu,Shijia Tang,Qiao Zhou,Haonan Ding,Feimin Zhang,Ning Gu
Placing a barrier membrane between an alveolar bone wound and gingival soft tissue is a common and effective method to prevent fibroblast invasion and promote new bone formation. However, the combined use of a barrier membrane with bone-forming substitutes remains a challenge for dentists during surgery. Thus, a Janus fibre/sponge composite combining iron oxide nanoparticles (IONPs, γ-Fe 2 O 3 ) was fabricated to prevent epithelial invasion and promote alveolar reconstruction. In the scaffold, a poly (lactic-co-glycolic acid)/polycaprolactone (PP) electrospun layer was covalently bound with a chitosan sponge containing γ-Fe 2 O 3 by polydopamine. The resilient chitosan sponge can easily adhere to the wound surface and encompass haemostatic capability by swelling and forming blood clots to block bleeding. The introduction of IONPs promoted the osteogenesis of rBMSCs, as shown by ALP analysis and cytokine assay. The PP layer maintained its structure integrity and prevented epithelial cells from invading into blood clots or fibroblasts from penetrating through the critical pore size. Assessment with a rat model having one-sided calvarial bone injury further confirmed the effectiveness of Janus scaffolds in facilitating the bone regeneration in the postoperative areas.
研究领域:
牙科
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生物材料
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组织工程
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再生医学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Hui Wang,Jueying Yang,Wei Tian,Kelin Peng,Yun Xue,Haosen Zhao,Xilan Ma,Rui Shi,Yu Chen
Recently, injectable dual-crosslinked (DC) hydrogel scaffolds have attracted many attentions as a class of excellent bone regeneration biomaterials with in-situ tunable functions. However, the design of injectable DC hydrogels with cell behavior-compatible network structure and mechanical property remains a bottleneck. Herein, based on the in-situ gelling method, we constructed an injectable CMCS/PEG+SA/CaCl 2 (CPSC) chemical/physical DC hydrogel scaffold with tunable softness/hardness mechanical properties and good biocompatibility. The formation mechanism and properties of the CPSC hydrogel scaffold were investigated by FTIR , XRD, rheometry, and mechanical testing. It is found that proper softness/hardness mechanical properties can be obtained by adjusting the secondary network structure of the hydrogel. The CPSC hydrogel scaffold prepared under optimal conditions can effectively promote cell infiltration , nutrient transport, and the osteogenic differentiation of rat bone mesenchymal stem cells (rBMSCs). The in vivo experiments show that the rBMSCs-loaded injectable CPSC hydrogels with appropriate mechanical properties can effectively promote bone reconstruction . This study has provided important guidance for the construction of injectable DC hydrogels with adjustable softness/hardness to promote osteogenesis for bone defect repair.
研究领域:
材料科学
·
生物材料
·
组织工程
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生物医学工程
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骨再生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）