BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Weiran Mao,Junlin He,Zhiyong Tang,Chengli Zhang,Jun Chen,Jia Li,Chao Yu
A novel electrochemical immunosensor was proposed for the detection of monocyte chemoattractant protein-1 (MCP-1), a biomarker of cardiovascular disease. Due to thionine (Thi) possessing electroactive redox properties, a one-step approach was utilized to synthesize a reduced graphene oxide-tetraethylene-thionine-Au (rGO-TEPA-Thi-Au) nanocomposite at room temperature using the synergistic effect of Thi and rGO-TEPA towards HAuCl 4 . We obtained the excellent matrix material , which immobilized more primary antibody MCP-1-Ab 1 on rGO-TEPA on a modified glassy carbon electrode (GCE). To further enhance the sensitivity of the sensor, a novel signal generation and amplification strategy was developed for detection. RuPdPt trimetallic nanoalloy particles (RuPdPt TNPs), a novel nanomaterial , were synthesized by a one-pot method, displayed a uniform morphology as well as good electrochemical activity and bound with the secondary antibodies against MCP-1 via the Pt-NH 2 bond. Most importantly, RuPdPt TNPs have a significant ability to catalyze H 2 O 2 to produce an electron. The electrochemical signal was highly amplified because the electrochemical signal was primarily derived from the synergistic catalysis of H 2 O 2 by RuPdPt TNPs and recorded by chronoamperometry . Under the optimal conditions, this newly designed biosensor exhibited sensitive detection of MCP-1 in the range from 20 fg mL −1 to 1000 pg mL −1 , with a detection limit of 8.9 fg mL −1 (based on a S/N = 3). Additionally, the designed immunosensor showed acceptable selectivity , reproducibility and stability. This immunosensor is a promising strategy for analyzing clinical serum samples in the future.
研究领域:
生物化学
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纳米技术
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电化学
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生物传感器
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医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Shuang Zhao,Yuchan Zhang,Songtao Ding,Jingchuan Fan,Zhi Luo,Kunguo Liu,Qi Shi,Weichi Liu,Guangchao Zang
A reliable and non-invasive electrochemical immunosensor for nuclear matrix protein 22 (NMP22) was presented based on reduced graphene oxide-tetraethylenepentamine (rGO-TEPA) and gold nanoparticles‑platinum nanoparticles-metal organic frameworks (AuNPs-PtNPs-MOFs) nanomaterial . rGO-TEPA is a promising template to enhance the loading ability of electrode for its large specific surface area and abundance in amino groups. AuNPs-PtNPs-MOFs nanomaterial was synthesized by NaBH 4 reduction method and applied to immobilize biomolecules due to their good biocompatibility, well conductivity , and excellent H 2 O 2 electrocatalytic ability. The electrochemical response signal was primarily originated from the obstruction of immobilized NMP22 antigen towards electrocatalytic reduction of H 2 O 2 and the signal was recorded by differential pulse voltammetry . Under optimum conditions, the immunosensor showed a sensitive response towards NMP22 at a broad concentration range from 0.005 ng·mL −1 to 20 ng·mL −1 . Two strong linear ranges could be observed at 0.005 ng·mL −1 to 0.5 ng·mL −1 (R 2 = 0.9833), and 0.5 ng·mL −1 to 20 ng·mL −1 (R 2 = 0.9909), with a low LOD of 1.7 pg·mL −1 (S/N = 3). Satisfactory reproducibility, stability and selectivity were obtained for NMP22 detection in real urine samples compared with standard ELISA assay, demonstrating the good analytical performance and potential non-invasive clinical application of this label-free electrochemical immunosensor.
研究领域:
分析化学
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生物传感器
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纳米技术
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Shanshan Wei,Haolin Xiao,Miao Gu,Zhencheng Chen,Liangli Cao
In this study, a sensitive and quantitative label-free electrochemical immunosensor based on bimetallic [email protected] nanoparticles , Prussian blue (PB), and reduced graphene oxide-tetraethylene pentamine nanocomposite (rGO-TEPA) was constructed to achieve sensitive detection of HBsAg. A one-pot green method was used to synthesize interlocked rGO-TEPA/PB hybrid composites as high-performance materials with good conductivity . PB was the electron transfer mediator used to provide electrochemical signals. By utilizing rGO-TEPA as supporting material , the stability and intensity of PB were greatly improved. The incorporation of highly conductive [email protected] nanoparticles could further amplify the electrochemical signal, and the high adsorption capacity could provide an efficient substrate for hepatitis B surface antibody (HBsAb) immobilization. Under optimal conditions, the immunoassay had a wide linear range from 0.25 pg mL −1 to 400 ng mL −1 and a low detection limit of 0.08 pg mL −1 (S/N = 3). This method was also applied to the analysis of human serum samples, and the obtained results were on par with those of electrochemiluminescence immunoassay (ECLIA), suggesting significant potential for this new method in clinical research.
研究领域:
生物医学工程
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分析化学
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临床诊断
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 10
·1-10
·
IF 6.232
Cao Liangli,Xiao Haolin,Fang Cheng,Zhao Feijun,Chen Zhencheng
Using gold and magnetic nanoparticles co-decorated reduced graphene oxide-tetraethylenepentamine (rGO-TEPA/Au-MNPs) as the magnetic platform for capturing the primary antibody (Ab 1 ), separation and preconcentration of immunocomplex, a novel homogeneous electrochemical immunosensor was successfully developed. The newly prepared magnetic rGO-TEPA/Au-MNPs, compared with MNPs, exhibited better stability and enhanced electrical conductivity attributed to rGO-TEPA, and showed higher biorecognition efficiency due to AuNPs. In addition, Au@PtNPs were prepared and modified with secondary antibody (Ab 2 ) as an efficient signal probe for signal readout. Using carcinoembryonic antigen (CEA) as a model analyte, the prepared immunosensor demonstrated satisfactory properties like high stability, good repeatability and selectivity, wide linear range (5.0 pg mL −1 ~200.0 ng mL −1 ) as well as low detection limit (1.42 pg mL −1 ). The homogenous electrochemical immunosensor was applied to the detection of CEA in human serum and was found to exhibit good correlation with the reference method. Thus, the proposed rGO-TEPA/Au-MNPs-based homogenous immunoassay platform might open up a new way for biomarker diagnosis.
Graphical Abstract
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物医学工程
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Electrocatalysis
·
2020
·Vol. 11
·期 5
·513-521
·
IF 2.587
Xiao Haolin,Wei Shanshan,Chen Zhencheng,Cao Liangli
Herein we report a label-free amperometric immunosensor based on reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) hybrid material co-modified by gold and iron-oxide magnetic nanoparticles (Au/MNPs-rGO-TEPA) for detection of carcinoembryonic antigen (CEA). The Au/MNPs-rGO-TEPA nanomaterials were prepared and coated on the working electrode as a sensing platform for antibody (anti-CEA) immobilization and signal amplification. This nanomaterial exhibited some excellent functionalities, such as magnetic and enhanced electrical properties due to its large specific surface area and favorable conductivity. Thionine (Thi) molecule was attached onto rGO-TEPA via π-π stacking and was used as an electrochemically active substance. The immunosensor was developed based on the increase in quantity of CEA captured, proportional to the decrease in peak currents of Thi. Under optimal conditions, the Au/MNPs-rGO-TEPA/Thi-based immunosensor showed impressive linear range from 0.1 pg/mL to 150 ng/mL. In addition, the proposed method had excellent linearity, high sensitivity, and relatively good stability, thereby offering a good platform for early clinical analysis.
A novel label-free binary nanoparticle-decorated reduced graphene oxide-tetraethylene pentamine-based electrochemical immunosensor was developed. Au/MNPs-rGO-TEPA/Thi was used as electrochemical sensitive platform for quantitative detection of CEA.
研究领域:
生物传感器
·
纳米技术
·
分析化学
·
癌症诊断
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Haolin Xiao,Shanshan Wei,Miao Gu,Zhencheng Chen,Liangli Cao
Alpha-fetoprotein (AFP) plays an important role as a marker in the clinical diagnosis of cancer. However, the sensitive detection of AFP in clinical samples is still challenging. Therefore, we developed a highly sensitive electrochemical immunosensor for the effective detection of AFP. In this study, thionine (Thi) and gold nanoparticles (AuNPs) co-modified reduced graphene oxide-tetraethylene pentamine (rGO-TEPA-Thi-Au), which was used as the immunosensor platform to fix the primary antibody (Ab 1 ), leading to a large improvement in the sensitivity of the immunosensor. Furthermore, ordered mesoporous carbon (CMK-3) modified with AuPt binary nanoparticles ( [email protected] ) was used as the signal probe for further signal amplification because CMK-3 can provide a large specific surface area for in-situ reduction of AuPtNPs with good conductivity. Therefore, a dual signal amplification was observed in the amperometric i-t curve, and the detection limit of the proposed immunosensor was effectively enhanced. The constructed immunosensor showed a wide linear range of 0.005 to 100 ng mL −1 and a low detection limit of 0.0022 ng mL −1 at S/N = 3. Furthermore, the proposed immunosensor exhibited excellent stability and recovery, demonstrating its potential for clinical applications.
研究领域:
生物传感器
·
癌症诊断
·
纳米技术
·
电化学
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分析化学
·
材料科学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Shengzhong Rong,Lina Zou,Ye Zhu,Ze Zhang,Hongfeng Liu,Yingcong Zhang,Hui Zhang,Hongmin Gao,Hongjun Guan,Jing Dong,Yupeng Guo,Fenghai Liu,Xiaoxia Li,Hongzhi Pan,Dong Chang
Nuclear matrix protein 22 (NMP22) is a crucial bladder cancer marker, the accurate detection of which is conductive to improving the effectiveness of tumor prevention and treatment. However, due to low sensitivity, it is impossible for conventional immunoassay to detect the trace of NMP22 at the early stages of tumor progression. It is crucial to develop a simple, sensitive and accurate detection method of NMP22. As a variety of two-dimensional (2D) material, reduced graphene oxide-tetraethylene pentamine (rGO-TEPA) demonstrates excellent conductivity and contains abundant amino groups. As a sort of 3D material, silica-modified Cu-based metalorganic frameworks deposited silver nanoparticles ( [email protected] 2 @AgNPs) composites are characterized by large surface area, excellent biocompatibility and water stability, which allows the immobilization of anti-NMP22 in large amounts via Ag-N bonds. Through a combination of the advantages exhibited by 2D material, 3D material, AgNPs and disposable screen-printed electrode, a disposable and sensitive label-free electrochemical immunosensor has been successfully assembled for NMP22 detection. In addition to exhibiting a wide linear range (0.1 pg∙mL −1 -1000 ng∙mL −1 ) and a low detection limit (33.33 fg∙mL −1 , S/N = 3), the proposed immunosensor also demonstrated satisfactory selectivity, strong reproducibility and high stability. Moreover, the excellent performance in detecting human urine samples makes it applicable in practice.
研究领域:
生物医学工程
·
分析化学
·
癌症研究
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）