EUROPEAN JOURNAL OF PLANT PATHOLOGY
·
2015
·Vol. 142
·期 3
·547-556
·
IF 1.49
Luo Zaiqi,Guo Huili,Yang Yadong,Yang Mingfen,Ma Lanqin,Wang Younian
Huping jujube is widely cultivated in northern China. PcPKS5, a resveratrol synthase (STS) gene, contains all functionally divergent plant specific type III PKSs and is involved in resveratrol synthesis. This study aimed to assess the effects of heterologous overexpression of the resveratrol synthase (PcPKS5) gene on Huping jujube (Ziziphus jujuba Mill.) plant resistance and nutritional quality. Stems with leaves and shoot tips of Huping jujube (Ziziphus jujuba Mill) were infected with agrobacterium expressing PcPKS5 and GUS, and three positive plants were identified. Resveratrol (0.45 μg/g fresh weight) was detected by HPLC in line 2 transgenic plants. Interestingly, 100 μl extract of 1 g/ml transgenic plant crude extract showed an inhibitory effect equivalent to 1.0 μg of pure resveratrol. In addition, transgenic plants displayed increased anti-mite resistance compared with the wild-type counterparts. Importantly, higher PcPKS5 expression levels were found in young tissues compared with the stem and mature leaves. This study also provides a stable genetic transformation mechanism for developing new plant germplasms in Huping jujube.
研究领域:
植物科学
·
分子生物学
·
基因工程
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DOI
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 21
·3931
·
IF 3.06
Yuxiang Wang,Zeyu Kao,Ting Zhang,Yujun Zhang,Lili Qin,Zhihua Zhang,Bin Zhou,Guangming Wu,Jun Shen,Ai Du
The trans-resveratrol (RSV)-loaded silica aerogel (RLSA) was prepared by the sol-gel method, adding the drug during the aging process, solvent replacement and freeze drying. A series of characterizations showed that RSV stays in the silica aerogel in two ways. First, RSV precipitates due to minimal solubility in water during the solvent replacement process. After freeze drying, the solvent evaporates and the RSV recrystallizes. It can be seen from scanning electron microscope (SEM) and transmission electron microscope (TEM) images that the recrystallized RSV with micron-sized long rod-shaped is integrated with the dense silica network skeleton. Second, from small-angle X-ray scattering (SAXS) results, a portion of the RSV molecules is not crystallized and the size is extremely small. This can be attached to the primary and secondary particles of silica to enhance its network structure and inhibit shrinkage, which is why the volume and pore size of RLSA is larger. In addition, the diffusion of RSV in silica alcogel was studied by a one-dimensional model. The apparent diffusion coefficients of inward diffusion, outward diffusion and internal diffusion were calculated by fitting the time- and position-dependent concentration data. It was found that the outward diffusion coefficient (5.25 × 10−10m2/s) is larger than the inward (2.93 × 10−10m2/s), which is probably due to the interface effect. The diffusion coefficients obtained for different concentrations in the same process (inward diffusion) are found to be different. This suggests that the apparent diffusion coefficient obtained is affected by molecular adsorption.Keywords:RLSA;one-dimension diffusion;alcogel;adsorption/desorption;Silica Aerogel
研究领域:
药学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Royal Society Open Science
·
2018
·Vol. 5
·期 11
·
IF 2.504
Shuqian Wan,Long Zhang,Yunyun Quan,Kun Wei
Resveratrol (3, 4′, 5-trihydroxy-trans-stilbene, RSV), a nutraceutical, has recently attracted lots of attention because of its outstanding pharmacological potential. The effects of RSV on non-alcoholic fatty liver disease (NAFLD) remain inconclusive, although a wealth of research has been done. The major obstacle presented was RSV's poor bioavailability due to its poor aqueous solubility, chemical instability and intestinal metabolism. In this study, nanotechnology was used to encapsulate RSV to enhance its stability, water solubility and bioactivity, which can be used to treat NAFLD by HepG2 hepatocytes-induced in vitro. RSV-loaded poly (d, l-lactide-co-glycolide acid) (PLGA) nanoparticles (RSV-PLGA-NPs) were prepared according to an oil/water (O/W) emulsion technique. The RSV-PLGA-NPs were of spherical morphology with an average size of 176.1 nm and a negative charge of −22.6 mV. These nanoparticles exhibited remarkable encapsulation efficiency (EE%) (97.25%) and drug loading (14.9%) for RSV. A sustained RSV release from RSV-PLGA-NPs could be achieved especially in acidic conditions when simulating transporting through the gastrointestinal tract. In addition, these nanoparticles were stable enough to store at 4°C for a least six months with unchanged EE%. Moreover, RSV-PLGA-NPs were more efficient in alleviating lipogenesis, promoting lipolysis and reducing hepatocellular proliferation than free RSV due to its improved stability, water solubility and bioactivity. These findings indicated that the RSV-PLGA-NPs provided superb and stable drug delivery with small particle size, high capsulation efficiency, well-controlled drug release, which greatly enhanced the stability, water solubility and bioactivity. Besides, the discovery that the inhibitory effect of RSV-PLGA-NPs on hepatocellular proliferation and lipid accumulation in steatotic HepG2 cells may provide a new way to study the mechanism of NAFLD. Therefore, RSV-PLGA-NPs have a promising potential for NAFLD therapy.
研究领域:
药理学
·
纳米技术
·
肝病学
·
药物递送系统
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Cai Cheng,Yan Lin,Xin Chen,Yang Xie,Xing Jiang,Jinting Chen,Yanling Wu,Zaiyi Luo,Jiayi Xu,Zhirong Zhang,Changguang Wang,Pei Jing,Zhongbing Liu,Xingjie Du,Jun Jiang,Xiaoduan Sun,Xiangyu Zhou,Zhirong Zhong
Atherosclerosis, a leading pathological basis of cardiovascular diseases, is closely associated with oxidative stress, inflammation, and lipid metabolism disorders. Resveratrol has demonstrated anti-atherosclerotic activity, but its clinical application is limited due to poor solubility, short circulation time, and lacking targeting. To overcome these limitations and establish a multi-pathway synergistic strategy for treating atherosclerosis, we designed a stealth lipid nanosphere system with macrophage-targeted and pH-responsive properties. The complete lipid nanospheres were evaluated for biocompatibility, cellular uptake, antioxidant and anti-inflammatory activities, as well as the inhibitory effects on foam cell formation. Pharmacokinetics and targeting efficiency were analyzed using both high-performance liquid chromatography and an in vivo imaging system. Therapeutic efficacy and biosafety were assessed via serum biochemistry and histopathological examination. Results showed that the complete lipid nanospheres exhibited uniform spherical morphology, high encapsulation efficiency, and pH-responsive release. In vitro evaluations demonstrated excellent biocompatibility of the complete lipid nanospheres, along with selective uptake by activated macrophages. Furthermore, the complete lipid nanospheres exhibited potent antioxidant and anti-inflammatory effects, as well as significant inhibition of foam cell formation. In vivo studies demonstrated that the treatment significantly extended the plasma half-life of resveratrol while enhancing its targeted accumulation within atherosclerotic plaques. In ApoE −/− mice with atherosclerosis, this intervention exhibited multifaceted therapeutic effects, including attenuation of oxidative stress, suppression of inflammatory responses, and reduction of lipid deposition. The study concludes that the complete formulation, stealth folic acid-modified pH-responsive lipid nanospheres, can specifically target resveratrol to the activated macrophages in plaques and inhibit plaque progression with minimal side effects, primarily due to resveratrol's antioxidant, anti-inflammatory, and lipid metabolism regulatory activities.
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产品关联
DOI
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2025
·
IF 5.8
Yijun Zhou,Yanan Song,Ling Zhu,Hui Zhang
Resveratrol and curcumin, natural plant-derived polyphenols with antioxidant, anti-inflammatory, and anticancer properties, are limited by poor bioavailability and stability. Delivery systems offer an effective strategy to address these limitations. Notably, their co-encapsulation shows synergistic effects, significantly enhancing encapsulation efficiency, stability, and bioactivity. In this study, nanoparticles were prepared from pectin and sodium caseinate to deliver resveratrol and curcumin using a solvent evaporation method combined with electrostatic deposition, and their physicochemical and structural properties were characterized. The nanoparticles (PE-SC-C-RNP) had a narrow size distribution (247.57 nm), low polydispersity index (0.32), and high encapsulation efficiency (95.24 ± 0.76%). Structural analyses indicated that electrostatic interactions between pectin and sodium caseinate formed the nanoparticle outer layer, with resveratrol and curcumin in an amorphous state. In vitro digestion experiments showed that curcumin enhanced the binding of resveratrol to carriers without affecting the release rate of resveratrol or free amino acids, leading to a significant increase in antioxidant activity. Animal model experiments showed that the resveratrol concentrations were greater in the plasma of mice administered PE-SC-C-RNP than those administered nanoparticles encapsulating only resveratrol orally, with a 0.83-1.27-fold increase in the maximal tissue concentration, and better pharmacokinetics. The maximum tissue concentration of resveratrol increased 0.83–1.27-fold in the liver, kidney, and other tissues within 6.0 h of administration. These results demonstrated that pectin sodium caseinate nanoparticles and co-encapsulated curcumin effectively improved the bioavailability of resveratrol for the delivery of bioactive compounds.
研究领域:
药学
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食品科学
·
纳米技术
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生物化学
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营养学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2021
·
IF 5.89
Wang Wenrui,Zhou Mengyang,Xu Yang,Peng Wei,Zhang Shiwen,Li Rongjie,Zhang Han,Zhang Hui,Cheng Shumin,Wang Youjing,Wei Xinyu,Yue Chengxu,Yang Qingling,Chen Changjie
Multidrug resistance (MDR) is a serious problem during cancer therapy. The purpose of the present study was to formulate D-α-Tocopheryl polyethylene glycol 1000 succinate-resveratrol-solid lipid nanoparticles (TPGS-Res-SLNs) to improve its therapeutic efficacy against breast cancer. In this study, the solvent injection method was used to prepare the TPGS-Res-SLNs. It was found that the TPGS-Res-SLNs exhibited zeta potential and drug-loading of −25.6 ± 1.3 mV and 32.4 ± 2.6%, respectively. Therefore, it was evident that the TPGS-Res-SLNs can increase cellular uptake of chemotherapeutic drugs, induce mitochondrial dysfunction, and augment tumor treatment efficiency by inducing apoptosis. Moreover, it was found that SKBR3/PR cells treated with TPGS-Res-SLNs exhibited significant inhibition of cell migration and invasion, as compared with free resveratrol. In addition, results from in vivo SKBR3/PR xenograft tumor models revealed that TPGS-Res-SLNs has better efficacy in promoting apoptosis of tumor cells owing to high therapeutic outcomes on tumors when compared with the efficacy of free resveratrol. In conclusion, the findings of the present study indicate significant potential for use of TPGS-Res-SLNs as an efficient drug delivery vehicle to overcome drug resistance in breast cancer therapy.
研究领域:
药学
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肿瘤学
·
纳米技术
·
药物递送系统
·
癌症治疗
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2019
·Vol. 74
·期 5
·1377-1382
·
IF 1.246
Zhang Xue,Wang Yanhe,Zhang Zhuanzhuan,Xia Wenlong,Song Xiaofeng,Ma Liwei
Resveratrol which is a natural polyphenol with good biological activity is mainly used in medicine, but it has poor photostability in vitro and short plasma half-life in vivo. Multi-armed poly( ε -caprolactone) (PCL) has attracted great attention because the increased number of terminal group broadens the polymeric flexibility in biomedical application. In the work, six-armed PCL was coupled with resveratrol through the hydrolysable ester bond to solve its disadvantage and realize protection for more resveratrol. The synthesized product was characterized with 1 H NMR, GPC and DSC. The molecular weight of the designed polymer was less than the experimental, and the polymeric distribution of molecular weight was narrow. In addition, resveratrol changed the physical form of six-armed PCL. The resveratrol coupling with six-armed PCL presented better photostability compared to original resveratrol. The concentration curve of the coupled resveratrol extended to the plain terrain after 80 min of irradiation, while the original resveratrol continued to decline. They were 72.10% and 52.35% at 2 h, respectively. Moreover, six-armed PCL could effectively protect resveratrol in vivo, and buy time for reaching the target site. The in vitro released amount of the original resveratrol was 52.89% within 3 h, while the coupled resveratrol was only 6.28%. Then this study provides a promising strategy for efficient usage of resveratrol.
研究领域:
药学
·
高分子化学
·
生物医学工程
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Qing Cheng,Yanzhuo Lv,Deyu Li,Ning Li,Qiao Ding,Yinghai Zhao,Qi Zhao
The Sn-Fe alloy coating is a potential choice for decorative coatings and electronic products because of its excellent performance. However, oxidation of the bath is likely to occur in the process of use, which limits the production of electrodeposition of Sn-Fe alloy. Resveratrol has excellent antioxidant capacity and is considered as a non-polluting natural antioxidant. However, the understanding of using of resveratrol as stabilizer for electroplating solution in the field of electrodeposition is limited. In this study, resveratrol, hydroquinone and TBHQ are selected as the stabilizers of the electroplating bath to conduct comparative experiments for measuring the stability effect of resveratrol. The antioxidant capacities of three stabilizers are compared by means of the natural oxidation experiment, H 2 O 2 accelerated oxidation experiment and electrochemical testing. The natural oxidation experiment and H 2 O 2 accelerated oxidation experiment confirm that the antioxidant effect of resveratrol is higher than hydroquinone and TBHQ. To determine the optimal concentration of resveratrol in the electroplating solution, EIS and salt spray test were conducted. These results show that appropriate resveratrol can improve the corrosion resistance of the coating, but excessive resveratrol is detrimental to corrosion resistance. Therefore, the optimal concentration of resveratrol in the electroplating solution is 1.0 g/L.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Antioxidants
·
2022
·Vol. 11
·期 9
·1666
·
IF 7.675
Lei Wang,Congting Lai,Dong Li,Zisheng Luo,Lingling Liu,Yunbin Jiang,Li Li
Poor water solubility and low chemical stability, seriously limit the efficient bioavailability of resveratrol. Here, we propose encapsulating resveratrol in lecithin-polysaccharide self-assembled microspheres (LPSM). An LPSM was designed with a lecithin core, and alginate-carboxymethyl chitosan biolayer shell. The LPSM had a spherical shape with 12.171 ± 0.960 μm of particle size and −30.86 ± 1.37 mV of zeta potential. The introduce of lecithin remarkably increased the encapsulation efficiency of resveratrol to 92.78 ± 0.82%. The LPSM elevated the antioxidant capacity and ultraviolet resistance of resveratrol. Moreover, LPSM inhibited release in a simulated gastric environment, promoted sustained release in simulated intestinal environment, and elevated the bioavailability of resveratrol during in vitro simulated digestion. Results indicate that LPSM is promising as a carrier for resveratrol delivery to enhance stability and bioaccessibility.Keywords:resveratrol;encapsulation;soybean lecithin;polysaccharide;microspheres;stability
研究领域:
食品科学
·
药物递送
·
生物材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Nutrition
·
2022
·
IF 6.59
Zhao Guangshan,Yang Lian,Zhong Wenshen,Hu Yuze,Tan Yu,Ren Zhe,Ban Qiuyan,Yang Chung S.,Wang Yifei,Wang Zhiping
Resveratrol (RES) is considered to be an activator of AMP-activated protein kinase (AMPK) with many reported health benefits. Polydatin (POD) is a natural precursor and glycosylated form of RES. The glycoside structure of POD alters the bioactivity. Overnutrition-stimulated reactive oxygen species (ROS) promote the AMPK suppression and metabolic dysregulation. The present work compared the effects of POD and RES in ameliorating energy homeostasis imbalance in mice fed a high-fructose diet and elucidated the underlying mechanisms of action. Our results showed that POD elevated the fecal levels of valeric acid and caproic acid via modification of gut microbiota, while RES did not significantly influence the levels of fecal short-chain fatty acids (SCFAs). Both POD and RES markedly decreased the oxidative stress and activated the AMPK signaling pathways in the liver. POD and RES exerted a similar effect in alleviating glucose dysmetabolism, but POD was more effective in ameliorating lipid dysmetabolism than RES. Furthermore, valeric acid and caproic acid alone can activate the AMPK and ameliorate hypercholesterolemia, and enhance the effects of POD on improving lipid metabolism in mice. Overall, for the first time, we demonstrated that POD administration elevated the fecal levels of valeric acid and caproic acid by modifying gut microbiota, thus promoting AMPK activation may be the underlying mechanism that POD is superior to RES in alleviating the lipid dysmetabolism. Our results suggest that POD may be an alternative for RES as an AMPK activator.
研究领域:
药理学
·
营养学
·
代谢性疾病
·
胃肠病学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhenyu Wang,Yulin Lei,Xiwei Peng,Huifang Zhang,Huanqing Niu,Yong Chen,Chenjie Zhu,Dong Liu,Hanjie Ying
Resveratrol is an important natural product with a variety of biological activities. Plants and fruits are the primary dietary sources of resveratrol. To produce resveratrol more efficiently at a nutraceutical grade, a grape suspension cell culture was developed as an alternative resource for resveratrol. Subsequently, surface-adhesion-based immobilization of the grape cells was exploited to establish a continuous (repeated-batch) production process. The maximum resveratrol production by immobilized cells in the first batch culture reached 413 mg/L but dropped apparently in the second batch. A preimmobilization approach was further developed to enhance the grape cell adhesion on the carrier, which greatly improved the stability of continuous production. The immobilized cells were used continuously for 56 days throughout seven repeated batches with an average production of 163 mg/L, 2.2-fold higher than that of the suspension culture. This study demonstrated that surface adhesion of plant cells could be an effective method for the long-term continuous production of plant metabolites.
研究领域:
植物生物技术
·
生物化学工程
·
天然产物
·
医药生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
·
2014
·Vol. 140
·期 12
·2065-2075
·
IF 3.009
Li Peichun,Yang Sen,Dou Mengmeng,Chen Youran,Zhang Jie,Zhao Xiaoyan
Purpose The aim of this study was to investigate whether resveratrol (Res) combined with artemisinin (ART) possess synergistic effect on different cancer cells. Materials and methods The viability of HepG2 and HeLa cells treated with ART and Res was detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Combination index (CI) analysis and isobologram were used to assess the synergistic effect of ART and Res in different ratios. Wound-healing assay was used to investigate the migration rate. AO staining and fluorescent microscopy measurements were performed to detect the cell apoptosis. Reactive oxygen species (ROS) was measured with 2′7′-dichlorofluorescein diacetate (DCFH-DA). Results MTT assay indicated that ART and Res inhibited the growth of HeLa and HepG2 cells in a dose-dependent manner. The combination of ART and Res exhibited the strongest anticancer effect at the ratio of 1:2 (ART to Res). The combination of the two drugs also markedly reduced the ability of cell migration. Apoptosis analysis showed that combination of ART and Res significantly increased the apoptosis and necrosis rather than use singly. Additionally, ROS levels were elevated by combining ART with Res. Conclusions Taken together, the present study suggested that ART and Res possessed the synergistic anti-tumor effect. ART in combination with Res could be an effective therapeutic strategy for cancer.
研究领域:
肿瘤学
·
药理学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）