NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 10
·4667-4673
·
IF 3.3
Yufei Lei,Yujie Li,Chen Liu,Liqiong He,Qian Li,Lifu Liao,Xilin Xiao
A convenient and portable smartphone-assisted method for the colorimetric detection of uranyl ions (UO22+) in aqueous solutions is proposed using rifampicin and 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (Br-PADAP) as chromogenic agents. The addition of UO22+ would cause reaction with chromogenic agents to form complexes, changing the colour from orange to dark red in the rifampicin system and from yellow to light pink in the Br-PADAP system. To achieve the on-site estimation of the trace UO22+ concentration range, digital images of colour changes captured by the smartphone under fixed lighting conditions were imported into the built-in application named “Detect” to obtain HSV (hue, saturation, and value) and HSL (hue, saturation, lightness) information, which could be associated with the concentration of uranyl ions. For more precise laboratory-level detection, ultraviolet-visible (UV-vis) spectroscopy was employed as well. The absorbance of the rifampicin system exhibited strong linearity with the UO22+ concentration in the range from 4 to 50 μM at 375 nm with a method detection limit (MDL) of 3.17 μM by the UV-vis-based method, while that of Br-PADAP system had a linear relationship with the concentration from 0.9 to 7 μM at 562 nm with an MDL of 0.89 μM. The two strategies may be combined to detect UO22+ across a larger concentration range, and this method has been successfully used to analyze real samples including soil around uranium mines.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Flow Chemistry
·
2018
·Vol. 8
·期 3
·129-138
·
IF 1.658
Li Xin,Liu Zhuang,Qi Hao,Fang Zheng,Huang Siyu,Miao Shanshan,Guo Kai
To reduce the cost and improve the efficiency for rifampicin preparation, a continuous flow synthesis of rifampicin starting from rifamycin S and tert -butylamine was studied in a microreactor. Two reaction steps and one purification step were coupled in a microreactor, and rifampicin was obtained with 67% overall yield. This method used 25% less 1-amino-4-methyl piperazine and got 16% higher overall yield without changing solvent and purification process between steps. This method has a good potential for further industrial application.
研究领域:
制药工程
·
有机化学
✓ 已验证
DOI
高影响因子
Applied Nanoscience
·
2021
·Vol. 11
·期 8
·2271-2280
·
IF 3.674
Liu Yueqi,Li Chenxi,Chen Jia,Han Yufen,Wei Ming,Liu Jun,Yu Xiaomin,Li Fangjun,Hu Ping,Fu Lulu,Liu Yong
Rifampicin (RIF) and Isoniazid (INH) are two first-line drugs for tuberculosis treatment. Compared to isoniazid, rifampicin has poor water solubility, which results in low bioavailability. Combining the two drugs can improve the treatment effect of tuberculosis, but thier direct contact may accelerate the degradation of rifampicin, which is also considered to be another reason for RIF’s low bioavailability. In this study, the polyvinylpyrrolidone (PVP) was selected as the carrier and mixed with the loaded drug of rifampicin and isoniazid separately. The quick-released rifampicin/isoniazid-polyvinylpyrrolidone mingled fibers (RIPMF) were fabricated by the electrospinning method to promote the absorption of rifampicin. The results showed that isoniazid-polyvinylpyrrolidone (INH-PVP) nanofibers and rifampicin-polyvinylpyrrolidone (RIF-PVP) microfibers were mixed in RIPMF, instead of two drugs coexisting in one fiber. Rifampicin and isoniazid in the fibers changed from crystalline to amorphous states, which facilitated the release of the rifampicin from the fibers. The hydrophilicity of RIF-PVP fibers was not distinct, but that of RIPMF increased with the addition of INH-PVP fibers. The release rate of rifampicin in rifampicin-loaded fibers was twice as fast as rifampicin powder. Additionally, in vitro antibacterial test showed that RIPMF had an obvious inhibition effect on Staphylococcus aureus . Finally, the rifampicin-loaded fibers enhanced the release rate of rifampicin and improved its bioavailability. Increased bioavailability reduces the waste of rifampicin and prevents damage to the kidneys due to the massive metabolism of rifampicin in biomedicine.
研究领域:
药学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2024
·
IF 10.7
Xiaodi Yang,Xiyu Tang,Sisi Yi,Tao Guo,Yue Liao,Yan Wang,Xiangjun Zhang
Intracellular bacteria are recognized as a crucial factor in the persistence and recurrence of infections. The efficacy of current antibiotic treatments faces substantial challenges due to the dormant state formation of intracellular bacteria. In this study, we devised a strategy aimed at reverting intracellular dormant bacteria to a metabolically active state, thereby increasing their vulnerability to antibiotics. We found that oligosaccharides, especially maltodextrin (MD), can be absorbed by dormant S. aureus , leading to their revival and restoration of sensitivity to rifampicin (Rif). We then synthesized a reactive oxygen species (ROS)-responsive MD-prodrug by covalently binding MD with 4-(hydroxymethyl) phenylboronic acid pinacol ester (MD-PBAP) and prepared a ROS-responsive nanoparticles (MDNP) using a nanoprecipitation and self-assembly method. Once internalized by host cells, MDNP was degraded to MD, reactivating dormant S. aureus , and enhancing their susceptibility to Rif. More importantly, MDNP treatment restored the sensitivity of intracellular persistent S. aureus to Rif in both a reservoir transfer model and whole-body infection model. Additionally, MDNP have demonstrated excellent biocompatibility in both in vitro and in vivo settings. These results offer a promising therapeutic avenue for managing persistent intracellular bacterial infections by reviving and resensitizing intracellular dormant bacteria to conventional antibiotics.
研究领域:
药理学
·
微生物学
·
纳米技术
·
传染病学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF BACTERIOLOGY
·
2023
·Vol. 205
·期 8
·
IF 3.2
Meng-na Zhang,Xiao-ou Zhao,Qi Cui,Dao-mi Zhu,Muhammad Asif Wisal,Han-dong Yu,Ling-cong Kong,Hong-xia Ma
The development of novel antibiotic adjuvants is imminent because of the frequent emergence of resistance in Gram-negative bacteria, which severely restricts the efficiency and longevity of commonly used clinical antibiotics. It is reported that famotidine, a clinical inhibitor of gastric acid secretion, enhances the antibacterial activity of rifamycin antibiotics, especially rifampicin, against Gram-negative bacteria and reverses drug resistance. Studies have shown that famotidine disrupts the cell membrane of Acinetobacter baumannii and inhibits the expression of the outer membrane protein ompA gene, while causing a dissipation of the plasma membrane potential, compensatively upregulating the pH gradient and ultimately increasing the accumulation of reactive oxygen species by leading to increased bacterial mortality. In addition, famotidine also inhibited the efflux pump activity and the biofilm formation of A. baumannii. In the Galleria mellonella and mouse infection models, the combination of famotidine and rifampicin increased the survival rate of infected animals and decreased the bacterial load in mouse organs. In conclusion, famotidine has the potential to be a novel rifampicin adjuvant, providing a new option for the treatment of clinical Gram-negative bacterial infections.IMPORTANCE In this study, famotidine was discovered for the first time to have potential as an antibiotic adjuvant, enhancing the antibacterial activity of rifamycin antibiotics against A. baumannii and overcoming the limitations of drug therapy. With the discovery of novel applications for the guanidine-containing medication famotidine, the viability of screening prospective antibiotic adjuvants from guanidine-based molecules was further explored. In addition, famotidine exerts activity by affecting the OmpA protein of the cell membrane, indicating that this protein might be used as a therapeutic drug target to treat A. baumannii infections.
研究领域:
微生物学
·
药理学
·
传染病
·
抗菌素耐药性
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Longjie Liu,Qianyu Xu,Gary Owens,Zuliang Chen
Antibiotics are an emerging class of persistent contaminants that are now of major environmental concern because they pose potential risks to both environmental and human health. Here reduced graphene oxide composited with bimetallic iron/palladium nanoparticles (rGO@ n Fe/Pd) was synthesized via a green tea extract and used to remove a common antibiotic, rifampicin from aqueous solution. The innate physical rifampicin removal efficiency of the composite (79.9 %) was increased to 85.7 % when combined with Fenton-oxidation. The mechanism and the main factors controlling Fenton-oxidation of rifampicin by rGO@ n Fe/Pd were investigated. Oxidation followed a pseudo-second-order degradation kinetic model with an activation energy of 47.3 kJ mol −1 . rGO@ n Fe/Pd were characterized by Brunauer-Emmett-Teller (BET), fourier transform infrared (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray energy spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-Ray powder diffraction (XRD), and zeta potential. Rifampicin degradation products observed by LC-UV, where subsequently confirmed to be mainly 5,6,9-trihydroxynaphtho [2,1-b] furan-1(2 H)-one, 5,6-dihydroxy-1-oxo-1,2-dihydronaphtho [2,1-b] furan-2-yl formate and ( S )-5,6,9-trihydroxy-2-(3-methoxypropoxy)-2-methylnaphtho [2,1-b] furan-1(2 H)-one by LC–MS. Finally, the practical effectiveness of the composite material for antibiotic removal was demonstrated by the treatment of representative wastewaters, where rifampicin removal efficiencies of 80.4, 77.9 and 70.2 % were observed for river, aquaculture wastewater and domestic wastewater, respectively.
研究领域:
环境科学
·
材料科学
·
药物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2022
·
IF 5.545
Sun Xun,Chen Mingming,Lei Jiayu,Liu Xinran,Ke Xin,Liu Wengang,Wang Jingkuan,Gao Xiaodan,Liu Xin,Zhang Yun
In this study, β-CD@mesoporous SiO2 nanospheres (β-CD@mSi) were prepared by loading β-cyclodextrin (β-CD) onto mesoporous silica nanospheres through an in situ synthesis. This not only solved the defect of β-CD being easily soluble in water, but also changed the physical structure of the mesoporous silica nanospheres. FTIR and XPS results showed that β-CD was successfully loaded onto mesoporous silica nanospheres (mSi), while enhancing the adsorption effect. β-CD@mSi with a monomer diameter of about 150 nm were prepared. At a temperature of 298k, the removal efficiency of a 100 mg/L solution of rifampicin can reach 90% in 4 h and the adsorption capacity was 275.42 mg g−1 at high concentration. Through the calculation and analysis of adsorption kinetics, adsorption isotherms and adsorption thermodynamics based on the experimental data, the reaction is a spontaneous endothermic reaction dominated by chemical adsorption. The electron transfer pathway, structure–activity relationship and energy between β-CD@mSi and rifampicin were investigated by quantum chemical calculations. The accuracy of the characterization test results to judge the adsorption mechanism was verified, to show the process of rifampicin removal by β-CD@mSi more clearly and convincingly. The simulation results show that π–π interaction plays a major interaction in the reaction process, followed by intermolecular hydrogen bonding and electrostatic interactions.
研究领域:
材料科学
·
药学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2026
·
IF 5.2
Zhen Lin,Jialin Yang,Wensong Yao,Shan Lian,Xudi Zhuang,Xuemei Zhang,Yan Hu,Xinhua Lin,Wei Chen
Red-emissive carbon dots (R-CDs) with an appropriate HOMO energy level were synthesized. • A red-emission and glow-type R-CDs-peroxyoxalate CL system with high efficiency was developed. • Visual analysis of rifampin has been developed based on the CL system. • No complicated pre-treatment is required for the detection of RFP in real samples. • Selective detection of RFP in complex samples with good accuracy. Sensitive detection of Rifampin (RFP) is crucial for medication monitoring and food safety. Chemiluminescence (CL) is a significant detection method for RFP. However, most CL systems suffer from short luminescence durations and cannot enable visual detection. In this study, red-emissive carbon dots (R-CDs) with suitable HOMO energy levels and high fluorescent quantum yields were prepared. These R-CDs served as excellent energy acceptors and luminophores in a peroxyoxalate chemiluminescence (POCL) system, achieving a high CL quantum yield up to 4.59 × 10 −3 einsteins mol −1 . Based on the inhibitory effect of RFP on the CL emission of the POCL system, a visible CL sensor was developed for its rapid and sensitive detection. The method achieved a detection limit as low as 0.16 μM and a wide linear range of 0.25–50 μM. A visual analytical platform was further established, extending the measurable range to 0.5–80 μM. The strategy was successfully applied to the accurate determination of RFP in real samples, advancing nanomaterial-based CL sensing for applications in safety and health monitoring. Download: Download high-res image (110KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-10
·
IF 5.3
Yang Shuning,Tian Li,Feng Yongbo,Song Yujia,Li Ruidan,Guo Yanjia,Li Huiling,Li Chao,Lu Juan
A highly sensitive molecularly imprinted electrochemiluminescence (MIECL) sensor was developed for detecting rifampicin (RIF) based on luminol@Co-MOF. Co-MOF had a significant enhancement of ECL signaling in the luminol-O 2 system. Molecular imprinted polymers (MIPs) with the introduction of RIF provide new properties for the specific recognition of RIF. A noteworthy decrease in ECL intensity was observed with higher concentrations of RIF. Consequently, the ECL signal was controlled by RIF elution from and adsorption by the MIP, thus establishing a new method for RIF detection. Under optimal conditions, this sensor exhibited linear detection ranges of RIF between 1.0 × 10 −11 mol L −1 and 1.0 × 10 −6 mol L −1 , with a detection limit of 3.3 × 10 −12 mol L −1 ( S / N = 3). The recoveries ranged between 98.1 and 106.0% in fish samples. This method can be used as a sensitive and rapid method to detect RIF in real samples. Graphical
研究领域:
分析化学
·
药物检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2012
·
IF 2.102
Zhengqing Liu,Pengfei Yin,Huiping Gong,Pingping Li,Xiaodan Wang,Youqiu He
Aqueous glutathione (GSH)-capped CdTe / ZnS QDs with the diameter of 3–4 nm were synthesized. The fluorescence of CdTe / ZnS QDs at 577 nm was quenched in the presence of rifampicin (Rfp), with excitation wavelength at 350 nm. The mechanism of the interaction of CdTe / ZnS QDs with Rfp was investigated. Under the optimal conditions, the calibration plot of ln( F 0 /F ) was linear in the range 0.83–56 μg mL −1 with concentration of Rfp, and the detection limit was 0.25 μg mL −1 . The proposed method was successfully applied to the determination of Rfp in its commercial capsules, and satisfactory results were obtained. The recovery of the method was in the range 98.6–103.2%.
研究领域:
分析化学
·
药学
✓ 已验证
DOI
高影响因子
Biosensors-Basel
·
2026
·Vol. 16
·期 5
·236
·
IF 6.2
Jiahui Lin,Zhongping Mao,Fei Yan
Developing an ultrasensitive electrochemiluminescence (ECL) detection platform remains challenging due to the limited enrichment efficiency of ECL emitters and co-reactants at the electrode interface, as well as the insufficient catalytic enhancement of co-reactant conversion. Moreover, simultaneous in situ analyte enrichment and efficient anti-interference capability are often difficult to achieve in a single sensing interface. Herein, a new ECL platform was developed based on nanocatalyst-supported nanochannel-confined surfactant micelle (SM) system, which integrates an enhanced luminol-dissolved oxygen (DO) ECL response for the ultrasensitive detection of antibiotic rifampicin (RIF). A nanocomposite comprising nitrogen-doped graphene quantum dots and a molybdenum disulfide nanosheet (NGQDs@MoS2) was modified on an indium tin oxide (ITO) electrode. This nanocomposite layer catalyzed the oxygen reduction reaction (ORR), boosting the co-reactant efficiency of DO. Vertically ordered mesoporous silica film filled with surfactant micelles (SM@VMSF) was subsequently grown in situ on the NGQDs@MoS2surface. The hydrophobic micelles enable the simultaneous enrichment of luminol, DO, and RIF. Integrating the triple-enrichment effect of surfactant micelles with the high electrocatalytic effect of NGQDs@MoS2nanocomposite results in significant ECL enhancement of the luminol–DO. SM@VMSF also provides an excellent molecular sieving effect, endowing the sensor with high anti-interference capability and stability. RIF quenches the ECL signal by consuming superoxide anion radicals, enabling sensitive detection. Detection of RIF was established with a high sensitivity (2927 a.u. per nM) wide linear range (10 pM to 10 μM) and a low limit of detection (LOD, 2.5 pM). The fabricated sensor exhibits good selectivity and high fabrication reproducibility (relative standard deviation, RSD, of 1.9%). Additionally, the determination of RIF in eye drops and seawater samples was realized. This work offers new insights for the design of high-performance ECL sensing interfaces and sensitive detection of RIF.
✓ 已验证
DOI
高影响因子
TALANTA
·
2026
·
IF 6.7
Yuanjing Sun,Yanli Zhang,Wenfeng Zhuge,Yuxia Liu,Zhiying Yu,Jinyun Peng
Rapid and reliable determination of rifampicin (RIF) is important for dose optimization and therapeutic drug monitoring during tuberculosis treatment. Herein, In 2 O 3 /CdS heterojunctions were fabricated through calcination followed by in situ CdS growth and employed as an efficient photoactive interface for photoelectrochemical RIF sensing. The In 2 O 3 /CdS heterojunction enhanced photoinduced charge separation and provided an amplified photocurrent background, while RIF induced a concentration-dependent photocurrent quenching response for signal-off PEC quantification. Under optimized conditions, the proposed sensor showed a linear response to RIF over the concentration range of 0.010-75 μM, with a detection limit of 1.45 nM (S/N = 3). The sensor exhibited satisfactory selectivity against common coexisting substances, as well as good repeatability and storage stability. Accurate RIF quantification was further achieved in spiked human serum, urine, and pharmaceutical capsule samples, affording recoveries of 97.73% and 103.49% with relative standard deviations below 4.1%. This work provides a sensitive and reliable PEC platform for RIF determination in complex biological and pharmaceutical matrices.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）