Stress Biology
·
2023
·Vol. 3
·期 1
·1-16
·
IF 0
Zhao Haipeng,Ding Xiangyu,Chu Xiaomeng,Zhang Haimiao,Wang Xinyu,Zhang Xinwen,Liu Haoqi,Zhang Xiaoying,Yin Ziyi,Li Yang,Ding Xinhua
Gray mold is a destructive disease caused by Botrytis cinerea , a pervasive plant pathogen, which poses a threat to both tomato growth and postharvest storage. The utilization of induced resistance presents a potential strategy for combating plant pathogenic attacks. ZNC (zhinengcong), an extract derived from the endophytic fungus Paecilomyces variotii , has been discovered to play a vital role in preventing diverse forms of bacterial infections. Nevertheless, the precise mechanism behind its ability to enhance tomato resistance to fungi remains unclear. In this study, we found that the exogenous spraying of ZNC could significantly improve the resistance of tomato plants to B. cinerea . The results of both the metabolomic analysis and high-performance liquid chromatography (HPLC) demonstrated that tomato plants responded to ZNC treatment by accumulating high levels of rutin. Additional transcriptome analysis uncovered that rutin enhances tomato resistance possible by initiating the generation of reactive oxygen species (ROS) and phosphorylation of mitogen-activated protein kinases (MPKs) related genes expression during the initial phase of invasion by B. cinerea . In addition, we also found that rutin might activate plant immunity by eliciting ethylene (ET) and jasmonic acid (JA)-mediated pathways. Therefore, plant immune inducer ZNC and rutin has bright application prospects and high utilization value to control gray mold. Graphical Abstract
研究领域:
植物病理学
·
分子生物学
✓ 已验证
DOI
TALANTA
·
2010
·
IF 3.29
Huan Zeng,Yuzhi Wang,Jinhuan Kong,Chan Nie,Ya Yuan
An ionic liquid-based microwave-assisted extraction (ILMAE) method has been developed for the effective extraction of rutin from Chinese medicinal plants including Saururus chinensis (Lour.) Bail. (S. chinensis) and Flos Sophorae. A series of 1-butyl-3-methylimidazolium ionic liquids with different anions were investigated. The results indicated that the characteristics of anions have remarkable effects on the extraction efficiency of rutin and among the investigated ionic liquids, 1-butyl-3-methylimidazolium bromide ([bmim]Br) aqueous solution was the best. In addition, the ILMAE procedures for the two kinds of medicinal herbs were also optimized by means of a series of single factor experiments and an L 9 (3 4 ) orthogonal design. Compared with the optimal ionic liquid-based heating extraction (ILHE), marinated extraction (ILME), ultrasonic-assisted extraction (ILUAE), the optimized approach of ILMAE gained higher extraction efficiency which is 4.879 mg/g in S. chinensis with RSD 1.33% and 171.82 mg/g in Flos Sophorae with RSD 1.47% within the shortest extraction time. Reversed phase high performance liquid chromatography (RP-HPLC) with ultraviolet detection was employed for the analysis of rutin in Chinese medicinal plants. Under the optimum conditions, the average recoveries of rutin from S. chinensis and Flos Sophorae were 101.23% and 99.62% with RSD lower than 3%, respectively. The developed approach is linear at concentrations from 42 to 252 mg L −1 of rutin solution, with the regression coefficient ( r ) at 0.99917. Moreover, the extraction mechanism of ILMAE and the microstructures and chemical structures of the two researched samples before and after extraction were also investigated. With the help of LC–MS, it was future demonstrated that the two researched herbs do contain active ingredient of rutin and ionic liquids would not influence the structure of rutin.
研究领域:
药学
·
分析化学
✓ 已验证
DOI
高影响因子
FOOD AND CHEMICAL TOXICOLOGY
·
2022
·
IF 5.572
Ahmet Şenocak,Vildan Sanko,Süreyya Oğuz Tümay,Yasin Orooji,Erhan Demirbas,Yeojoon Yoon,Alireza Khataee
MAX phases have attracted great attention due to unique features such as thermal and electrical conductivity, easy fabrication, heat resistant, and lightweight. In this study, an easy and green method was employed to successfully develop a Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase structure, and a Ti 3 Al 0.5 Cu 0.5 C 2 based glassy carbon electrode (GCE) was applied for the electrochemical determination of rutin antioxidants in mandarin and kiwi samples. The developed Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase was characterized by different techniques such as X-ray photoelectron spectroscopy (XPS), thermogravimetry and differential scanning calorimetry (TG-DSC), X-ray diffraction (XRD), Brunauer-Emmett-Teller (BET), diffuse reflectance spectroscopy (DRS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) to obtain information on the structural and morphological properties. Electrochemical methods such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were employed for the determination of rutin using Ti 3 Al 0.5 Cu 0.5 C 2 /GCE. The GCE modified with Ti 3 Al 0.5 Cu 0.5 C 2 demonstrated amplified electrochemical response (ca. 4.25 times) in comparison to the bare GCE towards rutin, and exhibited ultra-sensitivity and selectivity in the presence of other interfering antioxidants. Under the optimum conditions, good linearity in the range of 0.02–50.00 μmol L −1 was obtained for rutin analysis by the Ti 3 Al 0.5 Cu 0.5 C 2 -based sensor with a limit of detection (LOD, 3σ/K) as low as 0.015 μmol L −1 . The fabricated Ti 3 Al 0.5 Cu 0.5 C 2 MAX phase was applied to determine trace levels of rutin in mandarin and kiwi samples with validation by high-performance liquid chromatography (HPLC), thus highlighting its potential for the electrochemical determination of small molecules in the agricultural field.
研究领域:
食品科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ENTOMOLOGICAL RESEARCH
·
2024
·Vol. 54
·期 1
·e12702
·
IF 1.2
Ling Wang,Dong Xu,Yi Yang,Weiguo Liu,Shengbo Cong,Wenjing Li,Nina Yang,Haichen Yin,Lirong Jin,Huaiheng Wu,Peng Wan
The evolution of pest resistance to insecticidal proteins from Bacillus thuringiensis (Bt) restricts the sustainable application of transgenic Bt crops, and exploring other ecofriendly insecticides to efficiently suppress Bt-resistant populations is thus urgently needed. The plant secondary metabolite rutin possesses pest resistance effects and plays an important role in plant resistance to pests, but the effect of rutin on pink bollworms is unclear. We investigated the effects of rutin on two types (Bt-susceptible and Bt-resistant) of pink bollworms ( Pectinophora gossypiella ). Rutin had significant inhibitory effects on the growth, development, and oviposition of both Bt-susceptible and Bt-resistant strains. For both strains, rutin ingestion by larvae did not significantly affect larval survival at 7 days after treatment or egg hatching rate but significantly decreased pupation rate, eclosion rate, neonate-to-adult survival, pupal weight and eggs per female and significantly prolonged larval developmental periods, and the effect increased with increasing rutin content. The response to rutin ingestion was basically consistent between Bt-resistant and Bt-susceptible strains, indicating that the Bt-resistant strain did not exhibit cross-resistance to rutin. These results provide a reference basis for green control and resistance management of pink bollworms and may be used as a component of comprehensive management for this pest.
研究领域:
昆虫学
·
植物化学
·
害虫管理
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2023
·Vol. 47
·期 42
·19760-19766
·
IF 3.3
Bowen Zha,Ling Zheng,Sining Zheng,Liangbin Lin,Hongyu Guo,Fafu Yang
Rutin hydrate has some pharmacological properties and is widely available in human food and household samples. Although many methods based on large-scale instruments have been established for the detection of rutin hydrate, a simple, rapid and in situ measurement strategy was rarely reported up to now. In this work, a fluorescent sensor based on the cyanostilbene macrocycle (BCM) was developed for detecting rutin hydrate with high selectivity and sensitivity. BCM is a bis-cyanostilbene macrocycle with a bridging thiourea chain prepared by “1 + 1” condensation in 75% yield. BCM possessed a good fluorescence emission at 475 nm with an absolute fluorescence quantum yield of 0.26 in DMSO–H2O (5 : 95). It exhibited good selective detection for rutin hydrate among a series of normal ions and biomolecules with a “turn-off” fluorescence response. The binding constant of BCM for rutin hydrate was 2.4 × 104 M−1 and the detection limit attained 1.16 × 10−7 M. The 1 : 1 sensing mechanism was clarified using Job's plot, and 1H NMR and MS binding spectra. The sensing ability of BCM for rutin hydrate was successfully applied in detecting rutin hydrate in a test paper, three fruits and experiments of standard addition recovery with the recoveries of 90–95%, implying the simple, rapid and in situ detecting potential of BCM for rutin hydrate in real samples and the environment.
研究领域:
分析化学
·
药理学
·
食品科学
✓ 已验证
DOI
高影响因子
ANALYST
·
2016
·Vol. 141
·期 20
·5792-5798
·
IF 4.033
Chunlei Wang,Qi Wang,Min Zhong,Xianwen Kan
Multi-walled carbon nanotubes (MWNTs) and boronic acid based molecular imprinting polymer (MIP) were successively modified on a glassy carbon electrode surface to fabricate a novel electrochemical sensor for rutin recognition and detection. 3-Aminophenylboronic acid (APBA) was chosen as a monomer for the electropolymerization of MIP film in the presence of rutin. In addition to the imprinted cavities in MIP film to complement the template molecule in shape and functional groups, the high affinity between the boronic acid group of APBA and vicinal diols of rutin also enhanced the selectivity of the sensor, which made the sensor display a good selectivity to rutin. Moreover, the modified MWNTs improved the sensitivity of the sensor for rutin detection. The mole ratios of rutin and APBA, electropolymerized scan cycles and rates, and pH value of the detection solution were optimized. Under optimal conditions, the sensor was used to detect rutin in a linear range from 4.0 × 10−7 to 1.0 × 10−5 mol L−1 with a detection limit of 1.1 × 10−7 mol L−1. The sensor has also been applied to assay rutin in tablets with satisfactory results.
研究领域:
分析化学
·
药物分析
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Meng Jiang,Lin Zhu,Zejun Zhang,Yijing Ai,Lina Zeng,Shuhai He,Lin Li,Wei Sun
In this article the ZIF-8 based metal-organic frameworks-derived porous carbon was mixed with aminated graphene to obtain the nanocomposite, which was used to modify the surface of glassy carbon electrode (GCE). SEM, XRD and FT-IR were used to characterize the nanocomposite, which exhibited the unique morphology with functional groups. The modified electrode was applied to the electrochemical detection of rutin. Compared with that of GCE, the current response of rutin increased obviously on the modified electrode. Square wave voltammetry was further applied to quantitative analysis of rutin with a linear relationship between the concentration and peak current in two sections from 0.25 to 0.7 μmol·L -1 and 0.7 to 1.5 μmol·L -1 with detection limit of 15 nmol∙L -1 . Consequently, the detection of real samples was realized by this method.
研究领域:
材料科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2012
·Vol. 137
·期 10
·2503-2512
·
IF 4.23
Huan Zeng,Yuzhi Wang,Chan Nie,Jinhuan Kong,Xiaojie Liu
The preparation of magnetic molecularly imprinted polymers (MMIPs) which can be used for the separation and purification of rutin from Chinese medicinal plants has been proposed. By applying the improved co-precipitation method, magnetic Fe3O4 particles were easily prepared, followed by the modification of TEOS and functionalization with –CHCH2. Using functionalized Fe3O4 particles as the magnetic cores, rutin as the template, and acrylamide as the functional monomer, MMIPs were synthesized by surface-imprinted polymerization under the protection of nitrogen gas and successive mechanical stirring at 60 °C for 24 h. Magnetic non-molecularly imprinted polymers (MNIPs) were also prepared with the same synthesis procedure as with MMIPs only without the presence of rutin. Magnetic particles were characterized by FT-IR, XRD, and TG analysis. And the selectivity of MMIPs was also investigated in detail. In addition, the performance of the MMIPs for the adsorption of rutin in the analysis of Chinese medicinal plants was assessed. The mean recoveries were 84.33% (RSD: 3.22%, n = 3) for Saururus chinensis (Lour.) Bail and 85.20% (RSD: 3.58%, n = 3) for Flos Sophorae, respectively, which showed that the prepared MMIPs with many advantages possess the value of practical application.
研究领域:
药物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Jiajia Xi,Hao Wang,Bihong Zhang,Faqiong Zhao,Baizhao Zeng
A molecularly imprinted polymer/Bi 2 S 3 /ZnIn 2 S 4 /ITO sensor was fabricated and used for the photoelectrochemical detection of rutin. The Bi 2 S 3 /ZnIn 2 S 4 composite was synthesized by solvothermal method and it displayed good photoelectrochemical property because of the matched energy levels of Bi 2 S 3 and ZnIn 2 S 4 . In addition, ZnIn 2 S 4 could improve the stability of the composite and avoid photocorrosion. The photocurrent signal of the Bi 2 S 3 /ZnIn 2 S 4 composite was much larger than that of Bi 2 S 3 and ZnIn 2 S 4 under visible-light irradiation. Moreover, rutin imprinted chitosan was prepared by mixing rutin and chitosan solutions, followed by simple cross-linking reaction with glutaraldehyde at room temperature. The molecularly imprinted material was combined with Bi 2 S 3 /ZnIn 2 S 4 /ITO to construct photoelectrochemical sensor, and the resulting sensor showed a low detection limit (i.e. 0.003 μM) and a wide detection range (i.e. 0.01–100.0 μM) for rutin. It also displayed high stability and selectivity. The sensor could be used for the detection of rutin in real samples.
研究领域:
分析化学
·
材料科学
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
Toxins
·
2024
·Vol. 16
·期 3
·121
·
IF 3.9
Hira Sayed,Qiongqiong Zhang,Yu Tang,Yanan Wang,Yongpeng Guo,Jianyun Zhang,Cheng Ji,Qiugang Ma,Lihong Zhao
Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level ofbcl-2, decreasing the mRNA expression level ofkiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity.
研究领域:
毒理学
·
生殖生物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 37
·16045-16053
·
IF 3.288
Yang Ma,Mingming Li,Keren Shi,Zhifeng Chen,Baozhong Yang,Deyu Rao,Xiawei Li,Wei Ma,Shaozhang Hou,Guojing Gou,Huiqin Yao
Based on semi-interpenetrating polymer network (semi-IPN) hydrogel films, a multiple stimuli-responsive film system composed of poly(acrylic acid) (PAA) and N,N-diethyl acrylamide (DEA), designated as PAA–PDEA semi-IPN films, was realized on a glassy carbon (GC) electrode surface with a one-step polymerization method. Rutin (RT), an electroactive probe, displayed invertible pH-, thermo- and SO42− concentration–sensitive cyclic voltammogram (CV) behaviors in the film system. This multiply sensitive electrocatalysis of RT at the PAA–PDEA films was employed to further investigate the multiple stimuli-responsive ON–OFF behaviors of RT. The scanning electron microscopy (SEM) images and control experiments showed pH-sensitive ON–OFF properties of RT, which could be ascribed to the protonated/deprotonated carboxylic (–COOH) groups of the PAA component in different pH buffers and the properties of RT itself. However, the confirmation change or phase transition of the PDEA hydrogel film between the coil and globular states led to thermo- and SO42− concentration–sensitive ON–OFF properties. According to these results, a 3-input/3-output logic gate and a 2-to-1 encoder were constructed successfully. This present system was fabricated for the first time, which may offer numerous possibilities for the design of new electrochemical drug sensors, drug-mimicking release processes and other medical applications. Furthermore, it also can contribute to the development of biomolecular or molecular computing.
研究领域:
材料科学
·
生物医学工程
·
电化学
✓ 已验证
DOI
高影响因子
TALANTA
·
2012
·
IF 3.794
Huan Zeng,Yuzhi Wang,Xiaojie Liu,Jinhuan Kong,Chan Nie
Molecular imprinted polymers (MIPs) were prepared using rutin as the template, different reagents as the functional monomer and different reagents as the cross-linker by solution polymerization. Several parameters that would influence the performance of MIPs were investigated including the type of functional monomer (single or double) and cross-linker (single or double), and the molar ratio of the template, the functional monomer and the cross-linker. The optimum synthesis conditions of MIPs were found to be bi-monomers (acrylamide-co-2-vinyl pyridine, 3:1) and bi-crosslinker (ethylene glycol dimethacrylate-co-divinylbenzene, 3:1). The ratio of the template, the functional monomer and the cross-linker was found to be 1:6:20. MIPs synthesized under these conditions were filled into the cartridges as the adsorbents of solid-phase extraction (SPE). A competition test was conducted to authenticate the selectivity and the specificity of molecularly imprinted solid-phase extraction (MISPE) for rutin using the mixture solution of standard rutin and its structural analogs including quercetin, naringenin and kaempferol. Compared with purchased SPE including C 18 , silica and PCX, MISPE showed better selectivity and enrichment property for rutin in the extracted solutions of Chinese medicinal plants than any others. The mean recoveries were 85.93% (RSD: 3.04%, n = 3) for Saururus chinensis (Lour.) Bail and 88.61% (RSD: 3.36%, n = 3) for Flos Sophorae, respectively, which indicated that the optimized rutin -MIPs possess the value of practical application.
研究领域:
药物化学
·
分析化学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）