ANALYTICAL LETTERS
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2023
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IF 2
Jian Huang,Guolin Yuan,Dongliang Cheng,Fei Xie,Xianru Xia,Xiandong Li,He An,Chunyan Peng
Atherosclerotic coronary heart disease (CHD) is ischemic and a worldwide health issue. The soluble suppression of tumorigenicity 2 (sST2) is a novel biomarker for atherosclerotic coronary heart disease. This work develops an immunosensor with a nanocomposite consisting of polyaniline (PANI) and MXene-Nafion for the specific and sensitive determination of sST2. PANI was first electropolymerized on a glassy carbon electrode (GCE) to enhance electron transfer. Subsequently, MXene-Nafion dispersion was drop cast on the PANI/GCE substrate to further increase the electrical conductivity and immobilize anti-sST2 which captures sST2 for detection. The current response as a function of sST2 concentration was measured by differential pulse voltammetry (DPV). Under the optimal conditions, this immunosensor demonstrates a linear range from 1 pg mL−1 to 1 μg mL−1 and a detection limit of 0.149 pg mL−1. The device offers high sensitivity, excellent specificity, satisfactory reproducibility, good stability, and has significant potential for detecting clinical biomarkers. In addition, the immunosensor is able differentiate between serum of healthy people and CHD patients.
研究领域:
医学诊断
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生物传感技术
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DOI
高影响因子
MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Xinzhu Huang,Jiaxuan Pu,Yang Liu,Yidi Zhang,Guanghua Li,Li Ma
Ce-MOF enabled dual-mode fluorescence/colorimetric sST2 detection. • A 3D-printed portable device provided smartphone-based readout. • The sensor exhibited high sensitivity and reliable analytical performance. • The platform demonstrated good selectivity and serum applicability. Soluble suppression of tumorigenicity 2 (sST2) is an important biomarker for the diagnosis and prognosis of heart failure. However, conventional sST2 assays generally require sophisticated instrumentation and trained personnel, limiting their applicability in point-of-care testing (POCT). To address this challenge, we developed an integrated dual-mode sensing platform for sST2 determination by exploiting the fluorescence and peroxidase-mimicking properties of cerium-based metal–organic frameworks (Ce-MOFs) in combination with the high specificity of aptamers. Under laboratory conditions, the fluorescence and colorimetric sensing systems exhibited excellent analytical performance, with linear detection ranges of 0.1–200 ng/mL and limits of detection (LODs) of 0.02 ng/mL and 0.01 ng/mL, respectively. Furthermore, a portable visual dual-mode device was fabricated using 3D-printing technology and integrated with a smartphone-based analytical application, enabling quantitative analysis within 1 h. The portable platform exhibited linear responses over the range of 1–180 ng/mL, with LODs of 0.11 ng/mL and 0.07 ng/mL in fluorescence and colorimetric modes, respectively. Recovery studies in spiked serum samples yielded satisfactory recoveries ranging from 93.15% to 105.18%. The developed platform also demonstrated excellent selectivity and strong agreement with established clinical assays. This integrated dual-mode POCT platform provides a rapid, sensitive, accurate, and cost-effective strategy for sST2 determination, demonstrating significant potential for decentralized heart failure screening, prognosis assessment, and point-of-care clinical monitoring. Download: Download high-res image (84KB) Download: Download full-size image
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DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2024
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IF 4.9
Ziyan Hu,Zhongbing Huang,Juan Wang,Jirui Wen,Ximing Pu,Qianlei Lang,Peng Yang,Zhongxiang Shao,Jia Hu,Jiang Wu,Guangfu Yin
Typical symptoms of acute myocardial infarction (AMI) and acute aortic dissection (AAD) are similar chest pain, leading to higher clinical misdiagnosis rate, evenly the mortality rate of patients. In this work, ZrO 2 @PEI-QDs nanospheres with higher fluorescence were prepared by sequential electrostatic adsorption of branched PEI chains and CdSe/ZnS-COOH QDs on hollow ZrO 2 nanospheres. Three multi-channel LFIA strips (on sST2 and cTnI, cTnI and h-FABP, HMGB1 and sST2, respectively) based on ZrO 2 @PEI-QDs for rapid and correct distinguishing of AMI and ADD were constructed. Their visual limit of detection (vLOD for sST2 and cTnI, cTnI and h-FABP, HMGB1 and sST2) within 10–15 min reached 0.075 and 0.1 ng/mL, 0.1 and 1 ng/mL, 1 and 0.1 ng/mL, respectively, which were all lower than the normal level of healthy people. Compared with previous studies, ZrO 2 surface adsorbs more QDs, the fluorescence intensity was increased by about 2.84 times, and vLODs of three strips based on the quantitative fluorescence analysis were also increased by up to one orders of magnitude. The test results of five clinical serum samples indicated that three multi-channel LFIA strips could quantitatively detect four biomarkers of chest pain, and successfully distinguish AMI patients from AAD patients. These results indicated that this set of multi-channel LFIA strips could become an effective tool for the early and accurate detection or distinguish of ADD and AMI.
研究领域:
纳米技术
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生物传感器
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医学诊断
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心脏病学
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DOI
高影响因子
ACS Sensors
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2023
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IF 8.9
Jimei Chi,Meng Su,Bingjie Xue,Lijun Cheng,Zewei Lian,Yang Yun,Xu Yang,Xue Wang,Hongfei Xie,Huadong Wang,Yuan Wang,Jie Du,Yanlin Song
With the demand for point-of-care testing (POCT) in cardiovascular diseases, the detection of biomarkers in trace blood samples is of great significance in emergency medicine settings. Here, we demonstrated an all-printed photonic crystal microarray for POCT of protein markers (named “P4 microarray”). The paired nanobodies were printed as probes to target the soluble suppression of tumorigenicity 2 (sST2) as a certified cardiovascular protein marker. Benefiting from photonic crystal-enhanced fluorescence and integrated microarrays, quantitative detection of sST2 is 2 orders of magnitude lower than that of a traditional fluorescent immunoassay. The limit of detection is down to 10 pg/mL with the coefficient of variation being less than 8%. Detection of sST2 via fingertip blood is achieved in 10 min. Moreover, the P4 microarray after 180 days of storage at room temperature showed excellent stability for detection. This P4 microarray, as a convenient and reliable immunoassay for rapid and quantitative detection of protein markers in trace blood samples, has high sensitivity and strong storage stability, which hold great potential to advance cardiovascular precision medicine.
研究领域:
生物医学工程
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临床化学
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心脏病学
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即时检测
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）