Scientific Reports
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2014
·Vol. 4
·期 1
·1-8
·
IF 5.078
Bai Yanfen,Bi Huiping,Zhuang Yibin,Liu Chang,Cai Tao,Liu Xiaonan,Zhang Xueli,Liu Tao,Ma Yanhe
Salidroside (1) is the most important bioactive component of Rhodiola (also called as “Tibetan Ginseng”), which is a valuable medicinal herb exhibiting several adaptogenic properties. Due to the inefficiency of plant extraction and chemical synthesis, the supply of salidroside (1) is currently limited. Herein, we achieved unprecedented biosynthesis of salidroside (1) from glucose in a microorganism. First, the pyruvate decarboxylase ARO10 and endogenous alcohol dehydrogenases were recruited to convert 4-hydroxyphenylpyruvate (2), an intermediate of L -tyrosine pathway, to tyrosol (3) in Escherichia coli . Subsequently, tyrosol production was improved by overexpressing the pathway genes and by eliminating competing pathways and feedback inhibition. Finally, by introducing Rhodiola -derived glycosyltransferase UGT73B6 into the above-mentioned recombinant strain, salidroside (1) was produced with a titer of 56.9 mg/L. Interestingly, the Rhodiola -derived glycosyltransferase, UGT73B6, also catalyzed the attachment of glucose to the phenol position of tyrosol (3) to form icariside D2 (4), which was not reported in any previous literatures.
研究领域:
生物化学
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合成生物学
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微生物学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Guosi Li,Shanyong Yi,Haijiao Wang,Hulin Qiu,Wei Wang,Leilei Gao,Qilin Xu,Bangxing Han,Xinjian Yin
Salidroside is a phenylpropanoid glycoside with wide applications in the food, pharmaceutical, and cosmetic industries; however, the plant genus Rhodiola , the natural source of salidroside, has slow growth and limited distribution. In this study, we designed a novel six-enzyme biocatalytic cascade for the efficient production of salidroside, utilizing cost-effective bio-based L-Tyrosine as the starting material. A preliminary analysis revealed that the poor thermostability of the Bacillus licheniformis UDP-glycosyltransferase (EC 2.4.1.384) Bl YjiC M6 is a bottleneck in the cascade. Therefore, a combined computational strategy was used to engineer it and finally obtained a mutant TSM6 (T304V/G307A/N309W/F123W/T344V/D271G) with a 134-fold longer half-life at 40 °C and a 13 °C higher T m app compared to M6. The integration of TSM6 into the cascade improved salidroside productivity significantly, while reducing residual intermediates. After further optimization, the whole-cell biocatalytic cascade achieved a high salidroside titer of 12.8 g·L −1 in a 5 L bioreactor, giving a productivity of 0.53 g·L −1 ·h −1 . This study provides a green and efficient biosynthetic process for salidroside production and highlights the potential of enzyme engineering to enhance the biocatalytic cascade.
研究领域:
生物化学
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酶学
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合成生物学
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Yang Rongling,Wang Yu,Zhao Xiangjie,Tong Zheng,Zhu Qianlin,He Xiaoxi,Wang Zhaoyu,Luo Hongzhen,Fang Fang
Salidroside, the main bioactive compound isolated from the plant source of Rhodiola rosea L, possesses broad-spectrum pharmacological activities, but suffers from the low cell membranes permeability and alimentary absorption due to its high polarity. Therefore, a whole-cell catalytic strategy for the synthesis of salidroside esters was explored to improve its lipophilicity. The results showed that Aspergillus oryzae demonstrated the highest biocatalytic activity among the microbial strains tested. For the synthesis of salidroside caprylate, the optimum conditions of reaction medium, Aspergillus oryzae amount, molar ratio of vinyl caprylate to salidroside and reaction temperature were acetone, 30 mg/ml, 10°C and 40°C, respectively. Under these conditions, the initial reaction rate was 15.36 mM/h, and substrate conversion and regioselectivity all reached 99%. Moreover, the results indicated that although various 6′-monoesters derivatives of salidroside were exclusively obtained with excellent conversions (96%–99%), the reaction rate varied greatly with different chain-length acyl donors. This study details an efficient and cost-effective biocatalytic approach for the synthesis of salidroside esters by using Aspergillus oryzae as a catalyst for the first time. Considering the whole cell catalytic efficiency and operational stability, this strategy may provide a new opportunity to develop green industrial processes production for ester derivatives of salidroside and its analogues.
研究领域:
药理学
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生物化学
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工业微生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2014
·
IF 3.107
Hailong Peng,Wenjian Li,Fangjian Ning,Lihua Yao,Mei Luo,Xuemei Zhu,Qiang Zhao,Hua Xiong
A novel amphiphilic chitosan derivative of N,N-dimethylhexadecyl carboxymethyl chitosan (DCMCs) was synthesized. The structure of DCMCs was confirmed via FT-IR and 1H NMR, and the critical micelle concentration (CMC) was investigated by fluorescence spectroscopy. The results indicated that DCMCs has hydrophilic carboxyl and hydrophobic methylene groups and the CMC value was 23.00 mg·L–1. The polymeric liposomes (DCMCs/cholesterol liposomes, DC-Ls) were developed, and its properties were evaluated. The DC-Ls exhibited multilamellar spheres with positive charge (+73.30 mV), narrow size distribution (PDI = 0.277), and good crystal properties. Salidroside was first to encapsulate into DC-Ls. Compared with traditional liposomes (phosphatidylcholine/cholesterol liposome, PC-Ls), DC-Ls showed higher encapsulation efficiency (82.46%) and slower sustained release rate. The in vitro salidroside release from DC-Ls was governed by two distinct stages (i.e., burst release and sustained release) and was dependent on the pH of the release medium. The case II transport and case I Fichian diffusion were the main release mechanisms for the entire release procedure. These results indicated that DC-Ls may be a potential carrier system for the production of functional foods that contain salidroside or other bioactive food ingredients.
研究领域:
药剂学
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食品科学
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生物材料
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产品关联
DOI
PubMed
高影响因子
CHEMICAL & PHARMACEUTICAL BULLETIN
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2011
·Vol. 59
·期 8
·1045-1047
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IF 1.507
Yibing Guo,Xiaohong Li,Yahong Zhao,Yongxing Si,Hui Zhu,Yumin Yang
Salidroside is a phenylpropanoid glycoside isolated from Rhodiola rosea L., a traditional Chinese medicinal plant, and has displayed a broad spectrum of pharmacological properties. In this paper, two analogues were prepared with the glucosamine and N-acetylglucosamine as glycosyl donor, 2-(4-hydroxyphenyl)ethanol as glycosyl acceptor. The effects of them over PC12 cell model exposed to hypoglycemia and serum limitation were assessed with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Flow Cytometry and Western blot analysis.
研究领域:
药理学
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神经生物学
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DOI
PubMed
高影响因子
International Journal of Electrochemical Science
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2023
·
IF 1.5
He Zheng,Wei Yan,Zhongfu Zuo,Xuezheng Liu
In recent years, molecular imprinting has emerged as a promising strategy for developing highly selective and sensitive sensors for various analytes. In this study, we present the fabrication and characterization of an electrochemical sensor based on the molecular imprinting strategy for the detection of salidroside, a bioactive compound found in Rhodiola rosea . The sensor employs a modified glassy carbon electrode functionalized with multi-walled carbon nanotubes (MWCNTs) as the working electrode. The molecularly imprinted polymer (MIP) was prepared using acrylamide as the functional monomer and MWCNTs as the supporting material. The MIP-based sensor exhibited exceptional sensitivity and selectivity towards salidroside, showing a linear correlation between the logarithm of salidroside concentration and the reduced peak current in the range of 1 nM to 1 μM, with a low detection limit of 0.51 nM. The sensor demonstrated excellent reproducibility and stability, with a relative standard deviation (RSD) of 3.1 % for parallel measurements and 94.8 % retained response after a 7-day storage period. The spiked recovery experiments for Rhodiola rosea samples yielded high accuracy and precision, with recovery rates ranging from 96 % to 99 % and RSDs of 1–4 %. The developed MIP-based electrochemical sensor offers a powerful and reliable approach for the detection of salidroside, holding great potential for applications in pharmaceutical and herbal medicine industries.
研究领域:
分析化学
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生物医学工程
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中药学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Xiaojing SI,Chaolin WANG,Li LI,Daixin YE,Youli WEI,Yaping DING
A facile and highly sensitive electrochemical sensor for salidroside was fabricated based on multilayer ultrathin films (UTFs) containing Ni–Al layered double hydroxide (LDH) and carbon fibers that obtained by electrospinning technology. The X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) images show that the film surface is synthesized successfully. In addition, we constructed the electrochemical sensor using the composite nanomaterials we prepared to achieve detection of salidroside and this sensor showed a good linear range (0.2–120 μM) and a low detection limit (0.067 μM). It has also been successfully applied to the detection of actual samples, compared with HPLC method, satisfactory recovery rates have also been obtained.
研究领域:
材料科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
·
2017
·Vol. 50
·期 9
·748-753
·
IF 0.629
Zhaoyu Wang,Rongling Yang,Jizhong Wang,Yanhong Bi,Chun Zhu,Xiangjie Zhao,Linghong Nie,Wenqian Li
Regioselective enzymatic glycosylation of D-glucose and various structural alcohols for synthesizing β-D-glucopyranosides was successfully conducted. Among the β-glycosidases from seven potential plant seeds, the low-cost β-glycosidase from Prunus persica seed displayed excellent activity for salidroside preparation. Several crucial parameters, such as the solvent, buffer pH, buffer content, substrate molar ratio, enzyme dosage, and temperature were examined for yield optimization. Under the optimal conditions, the initial reaction rate and yield of salidroside were as high as 3.04 mM/h and 18.90%, respectively. Moreover, the alcohol acceptor recognition of the enzyme in synthesizing β-D-glucopyranosides was also investigated. The experimental data indicated that β-glycosidase activity and yield varied widely among all the tested alcohols due to the specific spatial structures of various acceptors.
研究领域:
生物化学
·
酶工程
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DOI
高影响因子
Cardiovascular Toxicology
·
2026
·Vol. 26
·期 8
·92
·
IF 3.3
Yan Chi,Liu Xiaohong,Zhang Xiaoyan,Wei Pei,Li Xuefang,Li Dongxu,Zhang Guhao,Li Meng,Zang Xiayan,Lu Guangjian,Lin Fei
Mobocertinib, a tyrosine kinase inhibitor approved for EGFR exon 20 insertion-mutated non-small cell lung cancer, induces cardiotoxicity manifesting as QT prolongation. Salidroside, a bioactive compound from Rhodiola rosea, exhibits cardioprotective properties. We investigated electrophysiological mechanisms underlying mobocertinib cardiotoxicity and salidroside's protective efficacy using Langendorff-perfused rat hearts and neonatal rat cardiomyocytes (NRCMs). Hearts were treated with escalating mobocertinib concentrations (214, 428, 856 nM) with or without salidroside (20 μM) pretreatment; cardiac parameters including heart rate, PR, JT, QTc intervals and conduction were mapped via high-resolution multichannel electrophysiology. NRCMs underwent chronic mobocertinib exposure with/without salidroside, followed by transcriptomic sequencing, qRT-PCR, and western blot validation. Mobocertinib concentration-dependently prolonged PR, JT, QTc intervals, increased conduction time/heterogeneity, reduced heart rate/velocity, and JT/QTc prolongation persisted after washout. Salidroside pretreatment markedly attenuated these abnormalities. In NRCMs, mobocertinib prolonged field potential duration and impaired conduction; salidroside normalized parameters. Transcriptomic analysis identified enrichment in calcium signaling, aldosterone synthesis, cGMP-PKG, and adrenergic pathways. Notably, Atp2b2 was upregulated in mobocertinib group but downregulated in salidroside combination group, whereas Agtr1b showed opposite pattern. Thus, salidroside mitigates mobocertinib-induced electrophysiological toxicity by modulating Atp2b2/Agtr1b-related signaling networks, providing a novel cardioprotective strategy against mobocertinib-associated cardiotoxicity. Graphical
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高影响因子
Frontiers in Aging Neuroscience
·
2022
·
IF 5.702
Cai Yawen,Chai Yuhui,Fu Yu,Wang Yingdi,Zhang Yiming,Zhang Xue,Zhu Lingpeng,Miao Mingxing,Yan Tianhua
Amyloid β-protein (Aβ) is reported to activate NLRP3 inflammasomes and drive pyroptosis, which is subsequently involved in the pathogenesis of neurodegenerative diseases, such as Alzheimer's disease (AD). To date, the pathogenesis of AD is unfortunately insufficiently elucidated. Therefore, this study was conducted to explore whether Salidroside (Sal) treatment could benefit AD by improving pyroptosis. Firstly, two animal models of AD, induced, respectively, by Aβ1-42 and D-galactose (D-gal)/AlCl3, have been created to assist our appreciation of AD pathophysiology. We then confirmed that pyroptosis is related to the pathogenesis of AD, and Sal can slow the progression of AD by inhibiting pyroptosis. Subsequently, we established the D-gal and Nigericin-induced PC12 cells injury model in vitro to verify Sal blocks pyroptosis mainly by targeting the NLRP3 inflammasome. For in vivo studies, we observed that Aβ accumulation, Tau hyperphosphorylation, neurons of hippocampal damage, and cognitive dysfunction in AD mice, caused by bilateral injection of Aβ1-42 into the hippocampus and treatments with D-gal combine AlCl3. Besides, accumulated Aβ promotes NLRP3 inflammasome activation, which leads to the activation and release of a pro-inflammatory cytokine, interleukin-1 beta (IL-1β). Notably, both Aβ accumulation and hyperphosphorylation of Tau decreased and inhibited pyroptosis by downregulating the expression of IL-1β and IL-18, which can be attributed to the treatment of Sal. We further found that Sal can reverse the increased protein expression of TLR4, MyD88, NF-κB, P-NF-κB, NLRP3, ASC, cleaved Caspase-1, cleaved GSDMD, IL-1β, and IL-18 in vitro. The underlying mechanism may be through inhibiting TLR4/NF-κB/NLRP3/Caspase-1 signaling pathway. Our study highlights the importance of NLRP3 inflammasome-mediated pyroptosis in AD, and how the administration of pharmacological doses of Sal can inhibit NLRP3 inflammasome-mediated pyroptosis and ameliorate AD. Thus, we conclude that NLRP3 inflammasome-mediated pyroptosis plays a significant role in AD and Sal could be a therapeutic drug for AD.
研究领域:
神经科学
·
药理学
·
分子生物学
·
炎症
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产品关联
DOI
PubMed
高影响因子
Chinese Medicine
·
2022
·Vol. 17
·期 1
·1-17
·
IF 4.546
Yi Qingqing,Liang Pengchen,Liang Dongyu,Cao Liou,Sha Shuang,Jiang Xinquan,Chang Qing
Background Microarc oxidation (MAO) on the surface of medical pure titanium can improve its histocompatibility, and loading drugs on the surface can resist excessive intimal hyperplasia.Methods In this study, salidroside (SAL) was loaded on the surface of porous titanium (Ti) with polydopamine (PDA) carrier. The effects of SAL on the osteogenesis and angiogenesis of Ti implants were studied by phalloidin staining, alizarin red staining, ALP staining, wound-healing assay, cell transwell assay, matrigel tube formation, and osteogenic and angiogenic genes and proteins expression detected by PCR and western blot in vitro. The bone defect model experiments in rats was established in vivo including X-ray, micro CT, hematoxylin and eosin staining (HE), immunohistochemistry (IHC), Goldner's trichrome analysis, Safranin O-fast green staining and determination of contents of TNF-α and IL-6 in serum.Results EDS and EDS mapping showed that SAL could be loaded on the surface of the MAO coating by PDA. A drug release experiment showed that SAL loaded on the Ti coating could release slowly and stably without sudden release risk. In vitro cell experiments showed that the SAL coating could promote the proliferation, morphology, calcification and alkaline phosphate activity of MC3T3-E1 cells. At the same time, it promoted the migration and tube formation of HUVEC cells. The SAL coating promoted osteogenesis and angiogenesis by promoting the expression of genes and proteins related to. In vivo experiments, HE and IHC showed that SAL significantly promoted the expression of COL-1 and CD31. Goldner's trichrome and Safranin O-fast green staining showed that SAL coating could increase the new bone tissue around the implantation site. The SAL coating had anti-inflammatory activity by reducing the levels of TNF-α and IL-6 in vivo.Conclusion Therefore, SAL could improve osteogenesis and angiogenesis in conjunction with the Ti-PDA coating.
研究领域:
生物材料
·
组织工程
·
骨科
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2026
·
IF 8.7
Yuting Fu,Anji Chen,Ziyi Fu,Qingjuan Zhang,Wuqiang Deng,Liuyun Jiang,Xiang Hu
Polysaccharide-based hydrogels with integrated bioactive functionality are highly desirable for wound dressing applications. Here, we report an injectable, self-healing hydrogel (OPC@Sal) fabricated via dynamic Schiff-base crosslinking between oxidized pullulan (OPL) and carboxymethyl chitosan (CMCS), with simultaneous incorporation of salidroside as a therapeutic agent. The gelation kinetics, mechanical properties, and microstructure were finely tuned by varying the OPL concentration at a fixed oxidation degree. The optimized OPC@Sal hydrogel exhibited rapid self-healing, excellent injectability, favorable biocompatibility, moisture retention, exudate absorption, and sustained salidroside release, along with pronounced antibacterial activity against E. coli and S. aureus . In vitro, the hydrogel effectively scavenged intracellular reactive oxygen species and suppressed the expression of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in LPS-stimulated macrophages. Based on the established in vitro effects, network pharmacology analysis was performed as a mechanistic interpretation tool, identifying key targets (TNF-α, IL-6, CASP3, IL-1β) through which salidroside exerts its multi-target regulation of wound healing. In a mouse full-thickness skin defect model, OPC@Sal significantly accelerated wound closure, promoted collagen deposition, and enhanced re-epithelialization. Collectively, this work establishes a fully polysaccharide-derived hydrogel platform that synergistically integrates structural functionality with intrinsic bioactivity. The molecular engineering of oxidized pullulan and carboxymethyl chitosan, combined with network pharmacology-guided active compound selection, offers a sustainable and scalable strategy for developing advanced wound dressings.
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高影响因子
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