JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2016
·
IF 3.169
Xialin Chen,Yujie Guo,Yuanjia Hu,Boyang Yu,Jin Qi
Salvianolate Lyophilized Injection (SLI), a traditional Chinese medicinal (TCM) preparation which is used to treat stroke, is composed of multiple salvianolic acids from the aqueous extracts of Salvia miltiorrhiza , and includes mainly protocatechualdehyde, rosmarinic acid, salvianolic acid B, salvianolic acid D, salvianolic acid E, diastereomer of salvianolic acid E, salvianolic acid Y, lithospermic acid and diastereomer of lithospermic acid. It is difficult to quantitatively control the quality of SLI using traditional high performance liquid chromatography due to the highly similar structure of these constituents including three pairs of diastereomers and the lack of commercial resources for most of these constituents as standards. Thus, a highly reproducible, fast, accurate and simple 1 H quantitative nuclear magnetic resonance (qNMR) method without the need for calibration curves and complex computation was established by optimizing the solvent system and acquisition parameters to simultaneously determine the nine salvianolic acids and mannitol in SLI. This method was validated and successfully used to determine 10 batches of SLI and the qNMR data were further analyzed with a vector including angle cosine and the partial least squares method for the quality control of SLI. The results indicated that qNMR can be used as a routine method for the quality control of SLI and may have potential in the quantification of diastereomers in other TCM preparations.
研究领域:
药学
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分析化学
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传统中医药
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产品关联
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2023
·
IF 8.6
Jiajia Su,Jun Qian,Weihuan Zeng,Yue Wang,Guoyin Kai
It is important to separate salvianolic acids from Danshen owing to their significant therapy-related activities of cardiovascular and cerebrovascular diseases. In this study, phenylboronic acid functionalized polyethyleneimine-intercalated montmorillonite (PAPIM) was successfully synthesized via intercalation and amidation to capture salvianolic acid A (SAA), salvianolic acid B (SAB) and rosmarinic acid (RA) effectively. Adsorption of three salvianolic acids kinetically follows a pseudo-second-order model. The maximum adsorption capacities can be determined to be 1.285, 0.692 and 1.154 mmol/g for SAA, SAB and RA by Langmuir adsorption model, respectively. Comprehensive factors of function groups of adsorbents and structure of phenolic acids result in the affinity order of adsorbent for phenolic acids: SAA > SAB > RA > other phenolic acids with simple structure. Different dominant processes lead to different adsorption thermodynamics of three salvianolic acids. It is found that the adsorption mechanism includes covalent bond, electrostatic interaction, hydrogen bond and π-π stacking interaction. Remarkably, boronic acid affinity and electrostatic interaction play the important role in the selective adsorption process, which is verified by density functional theory (DFT) calculations. This work highlights that PAPIM is a promising adsorbent to realize effective separation of salvianolic acids and provides an alternative potential strategy to selective separate certain compound with multiple synergetic interactions.
研究领域:
化学
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药学
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材料科学
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产品关联
DOI
高影响因子
Journal of Zhejiang University-SCIENCE B
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2016
·Vol. 17
·期 5
·391
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IF 1.303
Yun Xing,Le Cai,Tian-peng Yin,Yang Chen,Jing Yu,Ya-rong Wang,Zhong-tao Ding
The antioxidant activities and total phenolic content of fermented Salvia miltiorrhiza with fungus Geomyces luteus were investigated. The results revealed that G. luteus fermentation could significantly improve the antioxidant activity and total phenolic content of S. miltiorrhiza . The main antioxidant constituents were characterized by spectroscopic analysis as salvianolic acids. High-performance liquid chromatography (HPLC) quantification also showed the enhanced content of salvianolic acid B after fermentation. The present study suggests that G. luteus fermentations are effective in the S. miltiorrhiza salvianolic acids’ enrichment process.
研究领域:
药用植物学
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天然产物化学
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发酵工程
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DOI
PubMed
高影响因子
Analytical Methods
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2017
·Vol. 9
·期 38
·5580-5585
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IF 1.9
Xialin Chen,Jin Qi,Boyang Yu
Compound Danshen Dripping Pills (CDDPs) are a Chinese herbal medicinal preparation composed of Salviae miltiorrhizae Radix and two other Chinese medicines, Notoginseng Radix and Borneolum. The main components of CDDPs are protocatechuic aldehyde, danshensu, salvianolic acid A, rosmarinic acid, ginsenoside Rg1, ginsenoside Rb1, L-(−)-borneol and isoborneol. The complex composition of CDDPs, which contains phenolic acids, ginseng saponins and borneols, makes quantitative analysis by traditional methods difficult. Therefore, a fast and accurate 1H qNMR method was established that can simultaneously determine the eight main components of CDDPs. The qNMR method was well validated and successfully applied in the determination of ten sample batches. The vector cosine angle method was used for further analysis of the results to evaluate the sample quality. The results indicate that the qNMR method is suitable for the quality control of CDDPs and has potential as an easy alternative for the analysis of other complex Traditional Chinese Medicines (TCMs) that require two or more traditional instruments for comprehensive quantitative analysis.
研究领域:
药学
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分析化学
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中药学
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
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2018
·
IF 4.244
Qian Zhang,Dong-Dong Zhou,Jia-Wei Zhang,Die Gao,Feng-Qing Yang,Hua Chen,Zhi-Ning Xia
In this study, a novel amino-terminated supramolecular cucurbit [6] uril (CB [6]) pseudorotaxane motif coated magnetic nanoparticles (MNPs) was successfully fabricated through cucurbit [6] uril promoted azide-alkyne cycloaddition. The physicochemical properties of the obtained functional MNPs were investigated by scanning electron microscope, transmission electron microscope, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffractometer, vibrating sample magnetometer, thermal gravimetric-derivative thermogravimetric analysis , and Zeta potential analyzer. Due to the abundant carboxyl groups in the structure of CB [6] and amino residue on the surface of the pseudorotaxane motif MNPs, the prepared materials were employed to extract salvianolic acids (SAs) for the first time. A series of significant parameters in the extraction and elution process, including sample pH, acetonitrile percentage, ionic strength and extraction temperature, as well as eluent type and desorption time, were examined systematically. Based on the results, the electrostatic interaction , van der Waals interaction , hydrophilic interaction and hydrogen bonding formation between the analytes and sorbent were speculated. The equilibrium state of static adsorption study indicated the outstanding adsorption capacity of pseudorotaxane motif MNPs toward six SAs to be 15–80 mg/g, providing much better adsorption affinity for SAs compared with MNPs only modified with amino, and their adsorption behaviors were all fitted with Freundlich adsorption isotherm model. In addition, the developed pseudorotaxane motif MNPs offered good recyclability and stability. Taking advantage of these merits, four SAs from Salvia miltiorrhiza Bunge were successfully extracted using the prepared pseudorotaxane motif MNPs with relative recovery in the range from 95.1% to 106.5% and RSDs below 9.7%. These results demonstrated that the synthesized pseudorotaxane motif MNPs showed potential as a candidate in the extraction of SAs.
研究领域:
材料科学
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纳米技术
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分析化学
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药学
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产品关联
DOI
PubMed
高影响因子
Separation Science Plus
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2022
·Vol. 5
·期 8
·368-377
·
IF 0
Jian Gao,Lin Zhou,Lele Zhang,Xi Luo,Zhifei Fu,Guixiang Pan,Qiang Xu,Lifeng Han
In this work, an effective method for simultaneous isolation of eight compounds from Salvianolic Acids for Injection was developed by preparative high-performance liquid chromatography based on improved dry load injection. Firstly, the chemical structures of the obtained compounds were identified by mass spectrometry and nuclear magnetic resonance spectra. Subsequently, a sensitive liquid chromatography-tandem mass spectrometry method was established to precisely quantify the eight isolated target analytes. The quantitative results show salvianolic acid B as the most abundant component (460.06–514.68‰) and protocatechualdehyde (0.03–0.08‰) as the least. Finally, the antioxidant activities of the isolated compounds were further investigated. The 2,2-diphenyl-1-picrylhydrazyl-scavenging assay indicated that (7′ R , 8′ S , 8″ R , 8′′′ R )-epi-salvianolic acid B had the lowest half-maximal inhibitory concentration (5.05 μg/ml). This study reports on the development of an effective and rapid method for simultaneous isolation of multiple compounds from traditional Chinese medicine preparation, which could be used as reference compounds for further quality control and activities screening.
研究领域:
药学
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分析化学
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中药学
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产品关联
DOI
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Jingtong Xia,Minhua Qian,Jiahao Zhou,Zhaoyan Wang,Hongli Li,Lei Zhou,Qiaosheng Pu
ABSTRACT Salvianolic acids (SAs) are a class of natural active substances that have been widely used in clinical treatment and food adjuvant therapy. In this work, we found that SAs could form the ternary complex with borax and 2-hydroxypropyl-β-cyclodextrin (HP-β-CD), thereby sensitizing their weak intrinsic fluorescence to maximized 92-fold for salvianolic acid B (SAB). The formation of ternary complex was dynamic and could complete once mixed, and the fluorescence intensity remained stable within 3 h. On this basis, an integrated strategy of derivatization and separation was proposed for the sensitive analysis of SAs using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection. After the systematic investigation, a solution consisting of 20 mM borax and 20 mM HP-β-CD (pH 8.5) was selected as the running buffer. By the direct injection of SAs, the dynamic derivatization was realized in the capillary, and the baseline separation was achieved within 6 min, with the detection limits of 1.2-21.2 nM for four SAs (S/N=3). Then, the developed CE-LIF method was successfully applied to the quantitative analysis of SAs in four traditional ginsengs, including Salvia miltiorrhiza, Codonopsis pilosula, Panax quinquefolius and Panax ginseng with the recoveries ranging from 95.2% to 110.7%. Except for four target SAs, a large number of unknown electrophoretic peaks had also been observed in four ginsengs, and then were utilized for the identification of ginseng species via principal component analysis. Furthermore, a hypoxia/reoxygenation model was constructed using Rat cardiomyocyte H9c2 cells, and subsequently, the developed method was applied to continuously monitor the consumption of SAB in cell culture medium after its intervention.
研究领域:
分析化学
·
药学
·
生物医学工程
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植物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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