JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Zhuoqun Su,Xue Zhang,Wanxiao Wang,Di Wu,Yongning Wu,Guoliang Li
Meat adulteration detection is crucial for ensuring food safety, protecting consumer rights, and maintaining market integrity. However, the current methods face challenges in achieving multiplexed detection through efficient signal conversion and output. This study introduces a nanopore-based approach for the simultaneous detection of multiple meat adulteration. Leveraging the interaction between DADA dipeptide and vancomycin, we designed a series of biomimetic ssDNA reporters with DADA-Van tags for multiplexed signal output. These ssDNA reporters can generate highly distinctive current blockage signals, which can be discriminated simultaneously by the current waveform. Combined with PCR-based strand displacement, ssDNA reporters can be released from magnetic beads in the presence of the target gene and are analyzed using α-hemolysin (α-HL) nanopores for multiplexed signal output. The signal frequency for each target gene has a linear relationship with the specific concentration range (for duck: 1 × 10–3 ∼ 10 nmol/L, for pork: 1 × 10–2 ∼ 10 nmol/L, and for chicken: 1 × 10–3 ∼ 10 nmol/L). The limits of detection are as follows: 0.418 pmol/L for duck, 4.473 pmol/L for pork, and 0.531 pmol/L for chicken. This method effectively enables the simultaneous detection of adulterated chicken, pork, and duck meat in lamb samples and holds potential for broad applications in ensuring the authenticity and safety of meat products.
研究领域:
食品安全
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生物技术
·
分子诊断
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jingjing Kuang,Shengli Ruan,Yangkun Sun,Zeyu Wu,Jiayao Xu,Ting Zhang,Xiaofan You,Shiman Yang,Min Zhang,Hongyang Zhang,Ping Hu
Exosomes are extracellular vesicles that inherit molecular markers from their parent cells for the transfer and delivery of membrane and cytosolic molecules between cells. Exosome detection and protein profiling provide noninvasive access to disease diagnosis and treatment. The nanozyme designed in this paper contains polydopamine-functionalization of graphene oxide (rGO@PDA) units that can adsorb ssDNA aptamers and CeO 2 metal oxide nanosheet units with peroxidase activity. As auxiliary regulator, adenosine triphosphate (ATP) enhances peroxidase activity of rGO@PDA@CeO 2 under a wide range of pH (4–7.4), which breaks through the limitation that traditional nanozymes can only exhibit peroxidase activity under acidic conditions. The ssDNA aptamers, which are recognition probes of exosome, can be adsorbed on the surface of rGO@PDA by aromatic stacking, competing with ATP molecules. Thus, the ssDNA aptamer acts as the switch for colorimetric sensor to flexibly regulate the peroxidase activity of the rGO@PDA@CeO 2 under physiological conditions , and realizes sensitive detection of exosomes. This assay achieves a detection linear range of 0.46 × 10 7 –10 8 particle/mL and a limit down to 3.81 × 10 5 particles/mL. In addition, this aptasensor sensitively profiles six exosomal protein across five cell types and clinical samples, suggesting their potential in clinic diagnosis.
研究领域:
生物医学工程
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分子生物学
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纳米技术
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Nuo Duan,Wenhui Gong,Shijia Wu,Zhouping Wang
Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA library immobilized systematic evolution of ligands by exponential enrichment (SELEX) technique. After cloning and sequencing, five aptamer candidates were picked out for affinity and specificity assays based on a graphene oxide (GO) adsorption method. The results showed that the aptamer CLB-2 binds specifically against CLB with a dissociation constant, Kd, value of 76.61 ± 12.70 nM. In addition, an aptamer-based fluorescence bioassay was established for CLB analysis. The correlation between the CLB concentration and fluorescent signal was found to be linear within the range of 0.10 to 50 ng/mL with a limit of detection of 0.07 ng/mL. It has been further applied for the determination of CLB in pork samples, showing its great potential for sensitive analysis in food safety control.
研究领域:
食品安全
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Junhui Du,Haibin Liu,Hongshuo Chen,Chuanjin Cui,Wensi Zhang,Xuechao Zhang,Zhang Bin
A competitive electrochemical aptamer biosensor for the selective detection of Pb 2+ was developed in this study. The PAMAM@Au hybrid materials and the bioprobe MB-GNPs-ssDNA were synthesized with chemical methods . Modified electrode with carboxylated graphene oxide was prepared via electrodeposition . PAMAM@Au was immobilized on the modified electrode using the EDC/NHS crosslinker, and aptamer (Apt) was bound to PAMAM@Au hybrid materials using the glutaraldehyde crosslinker. The assembly of MB-GNPs-ssDNA/Apt/PAMAM@Au/GO/SPCE biosensors was achieved through immobilizing bioprobe onto electrodes, based on the theory of aptamer-complementary chain reactions. The fabricated biosensor was characterized through electrochemical experiments, SEM, AFM , TEM, XPS and FTIR . The biosensor's performance was evaluated in a series of Pb 2+ aqueous solutions by differential pulse voltammetry (DPV), and the results demonstrated this biosensor had excellent repeatability, stability, and specificity. The detection range spanned from 0.5 pM/L to 500 nM/L, with a detection limit of 5 fM/L. Consequently, the proposed biosensor can serve as a reliable analytical tool for the sensitive detection of lead ions .
研究领域:
生物化学
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分析化学
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DOI
高影响因子
iScience
·
2024
·
IF 4.6
Xuan Wu,Rong Xiang,Danting Yang,Xiaoyao He,Lu Zhu,Fangfang Sun,Hanqing Li,Ningning Pi,Yi Li
Global pandemic has emphasized the needs for advanced pathogen diagnosis in dealing with newly emerged infectious threats, including the Langya henipavirus (LayV). LayV, as an emerging zoonotic pathogen, has potential to cause pandemic, but lacks of rapid diagnostic tools, particularly at point-of-care level. Here, we leveraged the merits of CRISPR-Cas12a biosensing and established a highly sensitive LayV detection method. By integrating CRISPR-Cas12a with RPA, 10 copies/μL ultra-sensitive LayV RNA detection has been achieved at room temperature within 30 min. More importantly, this study developed a special horseradish peroxidase (HRP)-single-stranded DNA (ssDNA) reporter enabling CRISPR-Cas12a to detect LayV RNA without pre-amplification, achieving a sensitivity of 1,200 copies/μL detectable by the naked eye. These explorations can serve as accelerator for CRISPR-Cas biosensing toward rapid response for newly emerged biological threats, and also provide a method to realize simple, precise, and amplicon-free point-of-care pathogen screening for resource limited or underdevelopment regions.
研究领域:
分子生物学
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传染病学
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诊断技术
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Zhehan Yang,Zhenjia Yang,Xiaoshu Lv,Guangming Jiang,Xiaolong Zhang,Xiaodan Li,Xiaoyu Li,Ruo Yuan,Ying Zhuo
Quantifying trace markers in clinical samples with electrochemical impedance spectroscopy (EIS) biosensors is limited mainly by ubiquitous biofouling because traditional antifouling coatings suffer from low stability and unsatisfactory antifouling capability etc. Herein, a simple and effective strategy was constructed to achieve a highly specific EIS biosensing platform for quantifying protein and DNA in serum by using single-stranded oligonucleotides (ssDNA) as antifouling coating. To achieve the optimized antifouling capability of ssDNA coating, the influence factors of ssDNA structure are investigated by combining electrochemical experiments with theoretical calculations. The results demonstrated that the chemical adsorption of adenine on the Au surface is stronger than that of the other three bases, which endows the poly adenine-ssDNA (polyAn) coating with outstanding antifouling capability. Importantly, we found that the capture ligand structure and ssDNA length remarkably affected the antifouling capability of polyAn because of steric hindrance. As a proof of concept, EIS biosensors based on polyAn antifouling coating were developed to detect target DNA and nuclear factor kappa b p65 (NF-kB p65), respectively, exhibiting outstanding capability of detecting DNA and NF-kB p65 in human serum with low interference and good accuracy. Overall, the presented work may contribute to a deeper understanding of the influence factors of ssDNA-based antifouling coating and holds great insight into the construction of highly specific biosensors for quantifying disease markers in clinical samples.
研究领域:
生物传感器
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分析化学
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生物化学
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分子生物学
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临床诊断
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DOI
高影响因子
DYES AND PIGMENTS
·
2019
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IF 4.018
Muhammad Sohail Haroone,Yuehua Hu,Ping Zhang,Ruili Ma,Xiaorang Zhang,Qari Muhammad Kaleem,Muhammad Bashir Khan,Jun Lu
Nucleic acids , the hereditary material of living beings, perform a vital role in major life activities including growth, heredity, and variation. The genetic information is coded in specific nitrogenous bases , which are the key players in genetic transformation and identification of hereditary diseases. Telomeres are specialized DNA structures present at the end of eukaryotic chromosomes, consisting of repetitive non-transcribed sequences (TTAGGG) and some binding proteins . They protect the chromosomal ends from sticking to each other, and play a key role in the maintenance of genomic integrity. Currently, a number of methods are reported for nucleic acids and telomeres detection but they are not easy going. Therefore, development of sensitive and fast DNA-biosensors to measure the nucleic acids and telomeres sequences is of great importance. This paper describes a systematic study on the preparation of simple ultrathin films (UTFs) composite of fluorescent dye 4′,6-diamidino-2-phenylindole (DAPI), having binding properties in the minor groove of AT-rich DNA segments, blending with single-stranded DNA (ssDNA) and incorporation into layered double hydroxides (LDH) nanosheets using layer-by-layer self-assembly method. The resulting UTFs exhibits excellent sensitivity, selectivity and reversibility for long complementary ssDNA sequence on the basis of DNA hybridization and fluorescence enhancement strategy. The dynamic range was 3–20 μg/mL with a detection limit of 20 μg/mL. This new (ssDNA @DAPI/LDHs) n UTFs, could be an efficient hybrid composite with potential applications in the field of bioluminescent sensoring materials.
研究领域:
生物化学
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分子生物学
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DOI
高影响因子
MICROWAVE AND OPTICAL TECHNOLOGY LETTERS
·
2025
·Vol. 67
·期 6
·e70278
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IF 1.2
Xiang Li,Rui-jie Tong,Yu Wang,Lu-yao Wang
Dopamine, as a neurotransmitter, is essential for maintaining the function of the extrapyramidal nervous system. This article introduces an optical fiber sensor based on surface plasmon resonance (SPR) for detecting trace dopamine. The SPR effect is induced by an Au film coated on a plastic-cladding optical fiber. The specificity for dopamine is achieved through an aptamer, while graphene serves as a mediator between the gold-coated optical fibers and adapters. The sensor shows a linear sensitivity of 0.853 nm/log(M) over a dopamine concentration range of 10 −11 to 10 −4 M, with a detection limit of 1 × 10 −11 M. Compared with existing dopamine detection methods, the proposed sensor exhibits a lower detection limit, a sufficiently wide linear range, and broad application prospects in biosensing.
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DOI
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 190
·期 1
·1-10
·
IF 6.408
Zhu Jing,Xu Wenxing,Yang Ye,Kong Rongmei,Wang Junmei
A nanozyme sensor array based on the ssDNA-distensible C3N4 nanosheet sensor elements for discriminating multiple mycotoxins commonly existing in contaminated cereals has been explored. The sensor array exploited (a) three DNA nonspecific sequences (A40, T40, C40) absorbed on the C3N4 nanosheets as sensor elements catalyzing the oxidation of TMB; (b) the presence of five mycotoxins affected the catalytic activity of three nanozymes with various degrees. The parameter (A 0 -A) was employed as the signal output to obtain the response patterns for different mycotoxins with the same concentration where A 0 and A were the absorption peak values at 650 nm of oxTMB in the absence and presence of target mycotoxins, respectively. After the raw data was subjected to principal component analysis, 3D canonical score plots were obtained. The sensor array was capable of separating five mycotoxins from each other with 100% accuracy even if the concentration of the mycotoxins was as low as 1 nM. Moreover, the array performed well in discriminating the mycotoxin mixtures with different ratios. Importantly, the practicality of this sensor array was demonstrated by discriminating the five mycotoxins spiking in corn-free samples in 3D canonical score plots, validating that the sensor array can act as a flexible detection tool for food safety. Graphical abstract A nanozyme sensor array was developed based on the ssDNA-distensible C3N4 NSs sensor elements for discriminating muitiple mycotoxins.
研究领域:
分析化学
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食品科学
·
生物化学
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DOI
PubMed
高影响因子
IEEE TRANSACTIONS ON ELECTRON DEVICES
·
2025
·
IF 3.2
Yao Li,Yuying Zhou,Bing Chen,Shuaidi Zhang,Ke Hu,Peiyun Li,Congyan Lu,Jiawei Wang,Xiaonan Yang,Feng Zhang,Wenchang Zhang,Ling Li
This work proposes a dual-gate InGaZnO thin-film transistor sensor array for label-free and cross-scale multiple biomarkers detection, including single-stranded deoxyribonucleic acid (ssDNA) and protein CA125. We exploit dual gate modulation for threshold voltage and CMOS-based SAR-assisted digital-to-analog converter (DAC) VBG calibration circuit to eliminate initial VTH offset variation caused by different functionalizations, which improve the reliability of the multibiomarker sensor array. At the same time, receptor enhancement on the indium gallium zinc oxide (IGZO) sensing membrane was detected by fluorescence assay, realizing higher sensitivity for biosensor. We demonstrate the sensor array response for pH in static and real-time mode, realizing near-Nernst limit sensitivity (55.02 mV/pH). Furthermore, at the top-gate configuration, with low operation voltage (0.1 V) and low offset variation (about 2 nA), the IGZO sensor array exhibits great performance for biomarker sensing which achieves sensitivity up to 4.426%/decade for ssDNA and 1102 nA/decade for CA125, with the limit of detection (LoD) of 1.67 aM for ssDNA and 0.1 fg/mL for CA125. This biosensor array provides a potential approach to realize miniaturization and high-density integration of multidimensional-biomarker detection.
研究领域:
生物医学工程
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传感器技术
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分子生物学
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DOI
高影响因子
RSC Advances
·
2019
·Vol. 9
·期 60
·34958-34962
·
IF 3.049
Lu Zhou,Guojie Liu,Yang Wang,Jianling Liu,Yajie Zhang,Yong Ma
In order to improve drug efficacy, and reduce drug toxicity and side effects, a novel drug controlled release system was developed based on mesoporous silica nanoparticles (MSNs) with gold nanoparticles (AuNPs) acting as pore caps and short single-stranded DNA (ssDNA) oligomers as the linker. The synthesised composites were characterized by Fourier transform infrared spectroscopy (FTIR), powder X-ray diffraction (XRD), thermogravimetric analysis (TGA), zeta potential measurement, transmission electron microscopy (TEM) and UV-vis spectroscopy. The anticancer drug doxorubicin (Dox) was applied as a model drug to investigate the 808 nm near infrared (NIR) laser-controlled drug release behavior at different pH by fluorescence measurements. The investigation results demonstrate that this nanocarrier could achieve drug controlled release by external near-infrared (NIR) laser stimulation, which is expected to be applied in cancer therapy.
研究领域:
纳米技术
·
药学
·
癌症研究
·
材料科学
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Huili Tian,Nuo Duan,Shijia Wu,Zhouping Wang
Spermine contamination ranks as one of the food safety issues, it will cause some adverse reactions if the intake of spermine is excessive in human body. So it is of great significance to establish fast and efficient analysis method to detect spermine in foods. In this study, the spermine aptamers with high affinity and specificity were obtained by the capture systematic evolution of ligands by exponential enrichment (Capture-SELEX) technique. Forty-one aptamer sequences were obtained by cloning and sequencing, and were divided into eight families based on homology and secondary structure analysis. The affinity and specificity of candidate aptamers was analyzed by isothermal titration calorimetry (ITC) and fluorescence assay . The aptamers named APJ-6 was picked out as the optimal aptamer that recognizes spermine specifically with the Kd value of 9.648 ± 0.896 nM. In order to verify the practicability of the selected aptamers, the sensitive aptamer-based fluorescene assay was designed. Under optimized conditions, this aptasensor exhibited a low detection limit of 0.052 nM, as well as a linear within the range of 0.1–20 nM. Besides, it has been further applied for the determination of spermine in pork samples and the recoveries ranged from 86.45% to 98.15%, showing its great potential for sensitive analysis in food safety control.
研究领域:
分子生物学
·
食品科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）