Food & Function
·
2015
·Vol. 6
·期 10
·3291-3295
·
IF 2.791
Hui-da Wan,Yong-mei Xia
Enzymatic hydrolysis and transgalactosylation of stevioside (St) were investigated using a β-galactosidase from Sulfolobus sp. The hydrolysis yielded steviol as the main final product. Under the optimal transgalactosylation conditions, the highest conversion of stevioside was 87.3% with lactose as a donor, several galactosylated products (St-Gals) were obtained. Metal ions such as Na+, K2+, Ca2+, Ba2+, Mn2+ and Mg2+ (2 mM) did not affect the transgalactosylation activity, while Fe2+, Fe3+ and Cu2+ reduced the transgalactosylation activity of β-galactosidase to 64%, 33% and 18%, respectively.
研究领域:
生物化学
·
食品科学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2022
·Vol. 45
·期 19
·3763-3773
·
IF 3.614
Nana Chen,Mingli Huang,Qiaoge Fu,Chaoyi Shi,Ruili Guo
The separation and removal of stevioside from natural product steviol glycosides to obtain high-purity rebaudioside A is of great significance for the application of steviol glycosides in food, medicine, and other fields. Here, in order to explore the adsorbent pore structure suitable for the separation of stevioside and rebaudioside A, a hierarchically porous amino-functionalized metal–organic framework (HP-NH 2 -MIL-53) with an appropriate and narrow pore size distribution was prepared using a modulator-induced defect-formation strategy. The results showed that the hierarchically porous structure with micropores and mesopores increased the specific surface area and exposed amino groups compared with original metal organic framework (NH 2 -MIL-53), and the maximum adsorption capacity of HP-NH 2 -MIL-53 for stevioside and rebaudioside A was 233.89 mg/g. The narrow pore size distribution close to 3.80 nm promoted the screening effect, resulting in a maximum adsorption selectivity of 4.13. This work proves that when the pore size of the adsorbent is between 1.41 and 3.80 nm, it has a certain pore size screening effect on stevioside and rebaudioside A, and the hierarchically porous metal-organic frameworks provide a pre-design idea of adsorbent structure for the separation of natural products with molecular weight of 800–1000 Da.
研究领域:
食品科学
·
材料科学
·
天然产物化学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Zhuqing Dai,Jiangfeng Song,Ye Chen,Lei Feng,Yayuan Xu,Dajing Li,Caie Wu,Zhongyuan Zhang,Jun Liu
This study aims to develop novel lutein nanoparticles encapsulized by stevioside (LUT-STE, 165 ± 2 nm average particles size) and systematically evaluate its bioavailability. Multiple spectroscopy and NMR analyses showed lutein and stevioside could interact through hydrogen bonds, C H π interaction and van der Waals forces. Molecular docking simulation showed lutein was well distributed in the hydrophobic cavity of stevioside. Analyzed by Caco-2 cellular models, the transported amount of LUT-STE was 2.39 times that of lutein in 120 min with a P app (B → A)/ P app (A → B) value of 0.63 ± 0.04. Nystatin and dynasore significantly reduced the cellular uptake of LUT-STE by 41.3% and 57.7%, respectively. Compared with free lutein, LUT-STE increased the C max in mice plasma by 5.01-fold and promoted the accumulation in multiple organs. LUT-STE promoted the protein expressions of CD36, NPC1L1 and PPARγ in both cell and animal models. In conclusion, stevioside entrapment significantly promote the bioavailability of lutein through multiple transmembrane pathways.
研究领域:
药学
·
营养学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-8
·
IF 7.8
Luo Yingting,Li Yuxin,Song Xuening,Wang Yuzhuo,Liu Simiao,Ren Fazheng,Zhang Hao
Coenzyme Q10 (CoQ10) plays a vital role in aerobic respiration and cardiovascular diseases; however, its application is limited owing to poor water solubility. In this study, equimolar CoQ10 and stevioside (STE) formed a co-amorphous (CM) system by lyophilization, and its solubility was approximately 63 times higher than that of CoQ10. Through crystal, thermodynamic, and morphological characterization of the formula, the formation of the CM system was confirmed. The intermolecular interactions were investigated by spectroscopies. The relationship of 8 intermolecular interaction sites between the two was confirmed via molecular dynamics simulation, firmly indicating the strong intermolecular forces. Further, CM products remained stable even under accelerated storage conditions, equivalent to 1 year at room temperature. Meanwhile, the area under curve (AUC) values increased by 5 times in the in vivo bioavailability study. In conclusion, the CoQ10 was transformed into an amorphous structure by initially employing STE through intermolecular interactions to enhance solubility.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2015
·
IF 2.128
Abubakr Musa,Ming Miao,Mohammed A.A. Gasmalla,Tao Zhang,Ahmed Eibaid,Waleed Aboshora,Bo Jiang
In this study, effect of shaking velocity on mono-glycosyl-stevioside production by Leuconostoc citreum SK24.002 alternansucrase acceptor reaction was investigated, with four different level of shaking velocity (75, 100, 125 and 150 rpm) up to 24 h at 25 °C, using sucrose as donor and stevioside as acceptor. The results revealed that mono-glycosyl-stevioside yield significantly increased with increase in the reaction shaking velocity and reached maximum yield of 3.78 ± .02 mg/mL at 150 rpm shaking velocity after 6 h of reaction. And an increase in the reaction shaking velocity up to 150 rpm reduced the final reaction time and increased the productivity of mono-glycosyl-stevioside product such that the high quantity of mono-glycosyl-stevioside product was produced in only 6 h rather than 24 h. The mono-glycosyl-stevioside product was completely separated using macroporous resin AB-8 flowed by semi-preparative HPLC. Macroporous resin AB-8 showed high selectivity and capacity toward mono-glycosyl-stevioside. The structure of mono-glycosyl-stevioside was characterized, as 13-{[α- d -glucopyranosyl-(1→6)-β- d -glucopyranosyl-(1→2)-β- d -glucopyranosyl]oxy}kaur-16-en-19-oic acid β- d -glucopyranosyl eater, a corroding to extensive 1D and 2D NMR (1H and 13C, COSY, HSQC, HMBC) and mass spectral data.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2025
·
IF 9.6
Junying Zhang,Tianyu Shao,Hailiang Li,Luying Zhu,Lamyaa Albakr,Nial J. Wheate,Lifeng Kang,Chungyong Wu
Androgenetic alopecia (AGA) is a prevalent form of non-scarring hair loss. Standard treatments, which include minoxidil (MXD) tincture and foam, face challenges due to MXD's water insolubility and poor skin permeability. The result is extended treatment duration and reduced therapeutic effectiveness. This study utilized stevioside (STV), a natural sweetener derived from the Stevia plant, as a novel solubilizing excipient and microneedle (MN) material. A solid dispersion of STV with insoluble drugs is developed and molded into an MN patch. STV significantly increased MXD's solubility to 47 mg mL −1 in water, ≈18 fold higher than the control. STV solubilizes MXD by forming micelles in aqueous solution with a critical micelle concentration of 15 mg mL −1 . In vitro skin permeation studies showed cumulative drug release of 85% and 18% skin retention for the MN patch, which indicated excellent drug absorption into the skin. Animal studies demonstrated that the MN patch significantly promoted hair growth. There is a significant increase in hair follicle transition to the growth phase, which resulted in 67.5% coverage of the treatment area by day 35. Collectively, the results highlight the potential of the STV MN delivery system for the treatment of AGA.
✓ 已验证
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2019
·Vol. 13
·期 2
·1159-1165
·
IF 1.415
Musa Abubakr,Jiang Bo,Ma Haile,Gasmalla Mohammed A. A.,Abdalhai Mandour H.,Al-alfarga Ammar
In this work, alternansucrase enzyme from Leuconostoc citreum SK24.002 was used to produce di-glycosyl-stevioside through acceptor reaction. This was detected by high-performance liquid chromatography (HPLC) and was isolated using AB-8 macroporous resin and semi-preparative HPLC. With the help of mass spectroscopy, 1D NMR (H 1 and C 13 ) and 2D NMR (COSY, HMQC, HMBC) the di-glycosyl-stevioside structure was identified to be 13-{[α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-β-D glucopyranosyl-(1→2)-β-D-glucopyranosyl]oxy} kaur-16-en-19-oic acid β-D glucopyranosyl eater. In order to increase the modified stevioside (di-glycosyl-stevioside) product and reduce production time, the impact of velocity of shaking on the di-glycosyl-stevioside production was also evaluated, using 75–150 rpm for 24 h at 25 °C. The results showed that the di-glycosyl-stevioside production increased significantly (p < 0.05) from 0.35 ± 0.0.01 mg/mL for 75 rpm to 1.0667 ± 0.0152 mg/mL for 150 rpm at 6 h during the glycosylation reaction.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Shuwei Luo,Yan Zhang,Jiangfeng Song,Ying Li,Caie Wu,Chenchen Zhang
This study investigated the solubilizing capacity of glycosylated stevioside/hydroxypropyl-methylcellulose (stevia-G-HPMC) complexes with varying mass ratios on lutein . The impact on the steady-state flux and permeability coefficient of intracellular lutein was also explored through the construction of a Caco-2 cellular transport model. The results indicated that the equilibrium solubility of lutein linearly increased with an increase in stevia-G amount. The stability constants of the ternary system surpassed those of the binary system. Molecular dynamics simulation revealed a tight and stable structure in lutein supersaturated complexes. Meanwhile, lutein-stevia-G-HPMC complexes demonstrated superior cumulative penetrations, with the peak P app (AP → BL) value being (3.24 ± 0.89) × 10 −5 cm·s −1 . There was a slight decrease in P app (BL → AP), which improved the forward transport of lutein. Highly soluble lutein in aqueous environments saturated the extracellular transport proteins on the AP side of cell membranes, thereby maintaining the high permeability transport. Notably, the permeability trend of lutein in Caco-2 cells negatively correlated with the equilibrium solubility and matched the single exponential growth model. When the mass ratio of lutein, stevia-G and HPMC was 1:21:5, the solubility-permeability trade-off of lutein was effectively maintained.
研究领域:
药学
·
药物递送系统
·
生物药剂学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Qingqing Sun,Zhibin Dong,Zhihui Jiang,Mengran Wang,Mengzhen Xing,Xiaocen Wei,Yuning Ma,Lei Shi,Daoran Pang,Hehe Jiang,Yuxia Ma,Shuzhong Gao
Hydrogels derived from natural biomass are viewed as promising alternatives to petroleum-based fruit packaging due to the unique features of sustainable nature, biosafety, and environmental friendliness. However, biological macromolecules (e.g., polysaccharide and protein) generally lack antibacterial effect and UV shielding performance while existing hydrogels assembled from herbal small molecules (HSMs) are inadequate in mechanical properties, adhesion, and film-forming ability. To address this, we proposed a simple physical mixing method to construct supramolecular hydrogel based on tannic acid (TA) and stevioside (St) for fruit preservation. The monomers were proved to co-assemble into dense network structure driven by hydrogen bonding and π-π stacking interactions. Besides the good mechanical property, adhesion capability (87.55 kPa on polytetrafluoroethylene), and biocompatibility, the TA-St hydrogel exhibited excellent gas barrier (5.31 and 20.01 g/(m 2 ·h) for oxygen barrier and water vapor barrier, respectively), UV blocking performance (<350 nm), antioxidant activity (>86.66 %), and antibacterial ability (>99.99 %). Profiting from these comprehensive performances, the hydrogels were demonstrated to effectively reduce weight loss and maintain the fruit quality, significantly prolonging the shelf life to 12 days for blueberries. Notably, the TA-St hydrogel film possessing washability and biodegradability (5 days in soil) could be easily removed from the fruit surfaces. This work provides a new strategy to develop binary HSM-based supramolecular hydrogels with multifunctionality, boosting their applications in the fields of sustainable fruit preservation.
研究领域:
食品科学与技术
·
材料科学
·
可持续包装
·
超分子化学
·
采后生物学
✓ 已验证
产品关联
DOI
高影响因子
Canadian Journal of Chemistry
·
2020
·Vol. 98
·期 11
·
IF 1.171
Guanyu Wang,Qiaoge Fu,Ruili Guo,Zhong Wei
The boronic acid functionalization metal-organic frameworks (MOFs), as unique boronate affinity adsorbents, have desired specific molecular affinity for the separation and enrichment of cis-diol-compounds. Herein, the boronic acid functionalized Zn-based MOF adsorbent (MOF-BA) was synthesized through a simple one-step microwave method and used for the recognition and isolation of steviol glycosides (SGs). This MOF-BA exhibits the same spherical structure and isostructure with the parent framework composed only of the primitive ligand as verified by SEM and XRD characterization. It was confirmed that changing the ratio of ligands could achieve the adjustability of the boron content in the framework. At the same time, the MOF-BA-1.0 showed a suitable pore size (4.69 nm), and the presence of boric acid functional groups showed favorable selectivity for stevioside (STV). The static adsorption results showed that adsorption performances of rebaudioside A (RA) and STV from crude sugar solution (5.0 mg mL−1, pH 8) on MOF-BA-1.0 were investigated at 303 K for 15 h. The adsorption capacities for STV and RA were 42.93 mg g−1 and 22.96 mg g−1, respectively, and the adsorption selectivity (αSTV/RA) reached 4.35. The adsorption isotherm and kinetic data of MOF-BA-1.0 for RA and STV obeyed the Langmuir isotherm model and pseudo second order kinetic model, respectively. The study demonstrated that MOF-BA-1.0 adsorbent could be used as a potential adsorbent to purify the active ingredients of stevia and obtain a high concentration of RA products.
研究领域:
化学
·
材料科学
·
药学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Microorganisms
·
2025
·Vol. 13
·期 2
·216
·
IF 4.2
Samra Basharat,Lixin Zhai,Fuyao Jiang,Tanzila Asjad,Adil Khan,Xiangru Liao
The purpose of this research is to identify and characterize lactic acid bacteria (LAB) species in bee bread produced by honey bees (Apis Cerana) in the east mountain area of Suzhou, China. We isolated three strains,Apilactobacillus kunkeei(S1),Lactiplantibacillus plantarum(S2), andLacticaseibacillus pentosus(S3), with S2 producing the highest amount of lactic acid. Phylogenetic analysis indicated that these isolates, along with the type strain, formed a distinct sub-cluster within the LAB group. The strains exhibited non-hemolytic activity, lacked functional virulence factors, demonstrated high acid and bile tolerance, strong adhesion to intestinal cells, and antimicrobial activity against pathogens, collectively indicating their safety and high probiotic potential for therapeutic applications. Our studies demonstrated that S2 and S3 grew well in the presence of stevia leaf powder and steviosides, while S1 showed reduced growth and inhibitory effects. Importantly, the stevia-fermented strains exhibited strong probiotic potential along with significant antidiabetic, antioxidant, and antifungal properties in vitro. These findings highlight their potential applications in the food, feed, and pharmaceutical industries. Future research should focus on in vivo experiments to validate these results and evaluate compatibility among the strains before their application in functional foods.
研究领域:
微生物学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）