JOURNAL OF DRUG TARGETING
·
2019
·
IF 3.277
Lambert Nzungize,Md Kaisar Ali,Xiaoyu Wang,Xue Huang,Wenmin Yang,Xiangke Duan,Shuangquan Yan,Chunyan Li,Abualgasim Elgaili Abdalla,Ponmani Jeyakkumar,Jianping Xie
Tuberculosis, especially multidrug resistant cases, remains an enormous public health threat. Mycobacterium tuberculosis metC (Rv3340) an enzyme involved in methionine biosynthesis was identified and characterised for antimicrobial susceptibility. We reported that the overexpression of Rv3340 in Mycobacterium smegmatis (Ms_Rv3340) produces hydrogen sulphide (H2S) for its energy in harsh conditions. The produced H2S sustained Ms_Rv3340 against streptomycin, whereas the chemical inhibition of H2S caused streptomycin lethality to Ms_Rv3340. Further analysis showed that cysteine–H2O2 treatment of Ms-Rv3340 initiated DNA damage via Fenton reaction. Ms_Rv3340 downregulated the expression levels of three streptomycin responsive genes. To our knowledge, no study has been previously reported that M. tuberculosis metC (Rv3340) can generates H2S modulating resistant to streptomycin which provides a greater perception toward the treatment and control of tuberculosis.
研究领域:
微生物学
·
医学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2019
·
IF 6.044
Yuanhao Qiu,Chunbo Lu,Peng Chen,Feifei Sun,Dongdong Wang,Zhaojie Wang,Chunyan Hou,Haibo Mu,Jinyou Duan
Many pathogenic bacteria can invade phagocytic and non-phagocytic cells and colonize inside, which protects them from the attack by host immune system and antibiotics. A novel amphiphilic molecule was synthesized through conjugation of streptomycin and decylamine to hyaluronan. Rapamycin was encapsulted by the spontaneous self-assembly of hyaluronan-based amphiphilic molecules. The newly formed micelles not only facilitated the entry of drugs into host cells in part via CD44 phagocytic receptor, released streptomycin in the acidic compartment, but also could activate autophagy. The micelles elicited an efficient killing capacity against intracellular bacteria through the promotion of streptomycin uptake and rapamycin-initiated activation of autophagy. This strategy might highlight an acid-sensitive hyaluronan-based drug delivery system for effective treatment of intracellular infections.
研究领域:
药理学
·
微生物学
·
药物递送系统
·
传染病
✓ 已验证
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2024
·
IF 4.3
Jie Liu,Yinping Miao,Xiaolan Li,Mingpan Bi,Yanxi Wang,Yang Zhang
Streptomycin sulfate (STS) is one of the major sources of contamination in water. Excessive intake of STS can be a serious health hazard to humans, causing vestibular and auditory nerve damage, nephrotoxicity, myasthenia gravis or Parkinson’s disease. To address this, we propose an STS concentration sensor leveraging multiwalled carbon nanotubes (MWCNTs) and the vernier effect of a microfiber coupler (MFC). The vernier effect of MFC is caused by the interference of two supermodes in two orthogonal polarizations in a refringent optical fiber, and the high sensitivity can be achieved directly by monitoring the vernier envelope. MWCNTs can adsorb more biomolecules to enhance the light absorption of analytes to further improve the detection sensitivity. The influence of MFC structure parameters on sensor performance is analyzed by simulation and verified by experiment. Theoretical analyses and experimental results indicate that for specific diameter MFC, the sensor will have better performance. The sensor exhibits a remarkably high sensitivity of 1577.5 nm/(mg/ml) in the detection range of 0.02-0.1 mg/ml, namely sensitivity amplification factor nearly one order of magnitude, and the sensor achieves an ultralow limit of detection of 12.68 ng/ml. The vernier effect of MFC and the modification of MWCNTs effectively enhance the sensitivity of the STS sensor. The device does not require complex pretreatment or antibody preparation, making it highly promising for applications in food safety, environmental pollution, and health monitoring.
研究领域:
生物医学工程
·
环境科学
·
光学传感
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2016
·Vol. 40
·期 11
·9711-9718
·
IF 3.277
Junling Yin,Wenjuan Guo,Xiaoli Qin,Meishan Pei,Luyan Wang,Feng Ding
In this paper, a convenient electrochemical aptasensor for sensitive and selective determination of streptomycin antibiotic was constructed. Signal amplification strategy of the aptasensor was achieved by utilizing porous carbon nanorods, gold nanoparticles and copper oxide functionalized multiwalled carbon nanotube composites as biosensing substrates. Porous carbon nanorods were adopted in our strategy as the core conductive material for signal amplification. The multiwalled carbon nanotube–copper oxide–gold nanoparticle nanocomposites combined the advantages of their components, which not only promote electron transfer, but also provide abundant binding sites for the immobilization of biomolecules. The streptomycin aptamer was immobilized on the nanocomposites' surface via the strong bonding interaction between the gold nanoparticles and the thiol group to generate a referenced differential pulse voltammetry signal, Iaptamer. Then, the streptomycin was immobilized on the aptasensor via the specific interaction between the aptamer and streptomycin to generate the attenuated signal, Istreptomycin. Under the optimized conditions, the changes in the signals were linear with the concentration of streptomycin in the range of 0.05–300 ng mL−1 with a low detection limit of 0.036 ng mL−1 when “ΔI = Iaptamer − Istreptomycin was used as the response signal for quantitative determination of streptomycin. In addition, the electrochemical aptasensor was also successfully applied to the determination of streptomycin in milk and honey samples. This highly sensitive electrochemical aptasensor is of great practical importance in food safety and could be widely extended to the detection of other antibiotics by replacing the sequence of the aptamer.
研究领域:
分析化学
·
环境科学
·
食品安全
·
纳米技术
·
生物传感器
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 11
·8714-8721
·
IF 3.289
Haibo Mu,Qianjin Liu,Hong Niu,Yuelin Sun,Jinyou Duan
The emergence of biofilm-associated resistance of microbes to traditional antibiotics has resulted in an urgent need for novel antimicrobial agents. Herein we developed a facile approach to overcome the problem through chitosan–streptomycin gold nanoparticles (CA NPs). The synthesized CA NPs were characterized by ultraviolet-visible absorption spectra (UV-vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The resulting CA NPs maintained their antibiofilm activities towards Gram-positive organisms. More importantly, CA NPs damaged established biofilms and inhibited biofilm formation of Gram-negative bacteria pathogens. Mechanistic insight demonstrated that CA NPs rendered streptomycin more accessible to biofilms, thereby it was available to interact with biofilm bacteria. Additionally, CA NPs were observed to kill more biofilm-dispersed cells than CS conjugate or streptomycin and inhibit the planktonic cell growth of Gram-positive and -negative bacteria. Thus, this work represents an innovative strategy whereby gold nanoparticles linked to carbohydrate–antibiotic conjugates can overcome antibiotic resistance of microbial biofilms, suggesting the potential of using the generated CA NPs as antimicrobial agents for bacterial infectious diseases.
研究领域:
纳米技术
·
微生物学
·
药学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Yingying Liu,Jiao Du,Haowen Wu,Chang Cong,Haifeng Zhang,Jixiao Wang,Zhi Wang
Nanofiltration (NF) membrane with excellent dye/salt permselectivity and anti-fouling performance is highly desired for the separation of dye/salt mixture in dye wastewater treatment . In this work, the antibacterial NF membrane with high permselectivity and anti-fouling performance was prepared by using a hydrophilic antibiotic, streptomycin (SM) with abundant amino and hydroxyl groups, as the aqueous monomer via interfacial polymerization (IP) with trimesoyl chloride (TMC). The as-prepared antibiotic membrane had a hydrophilic and highly smooth surface, which was beneficial to the membrane anti-fouling performance. The membrane performance was optimized by tuning the concentration of SM, and the optimized membrane with desirable water permeance of 23.5 L·m −2 ·h −1 ·bar −1 had excellent dyes rejections (the rejections of Direct red 23 (DR 23), Congo red (CR), Acid red 66 (AR 66), Victoria blue B (VB), and Chrome black T (CBT) were 99.7%, 99.6%, 99.0%, 99.5%, 99.7%, respectively) and low salts rejection (the rejections of NaCl and Na 2 SO 4 were 1.9% and 4.5%, respectively). The optimized membrane also showed excellent separation performance to salt/dye mixed solutions and operational stability. This newly streptomycin-based NF membrane showed excellent application potential in the dye wastewater treatment.
研究领域:
环境科学
·
材料科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2026
·
IF 3
Dian Li,Shangbin Kao,Mengwei Li,Yuan Qin,Xiumei Zhou,Xueqin Zhao,Jie Chen,Biao Huang
This study developed a novel streptomycin immunodetection method based on the Amplified Luminescence Proximity Homogeneous Assay (AlphaLICA), termed STR-AlphaLICA, for the highly sensitive and rapid detection of streptomycin residues in infant formula. The method was established by conjugating streptomycin-bovine serum albumin (STR-BSA) to receptor microspheres and goat anti-mouse IgG antibody to donor microspheres, leveraging the principle of competitive binding between free streptomycin and the immobilized STR-BSA for specific monoclonal antibodies. In the homogeneous system, fluorescence signals are generated through singlet oxygen energy transfer upon 680 nm laser excitation, allowing direct detection without washing steps. The established standard curve demonstrated excellent linearity (r = 0.99977) within the range of 0.1–312.5 ng/mL, with a detection limit (LOD) of 0.169 ng/mL and a quantification limit (LOQ) of 0.776 ng/mL. Validation results showed excellent intra- and inter-batch precision (CV < 5.02%) and spiked recovery rates ranging from 91.21% to 106.64%. Cross-reactivity with the structural analog dihydrostreptomycin was 69.87%, while responses to other common antibiotics remained below 0.05%, indicating high specificity. Furthermore, validation results in five complex matrices—including honey and shrimp meat—further demonstrate the method's excellent universality and reliability. In actual sample testing, the results showed significant correlation (r²=0.9414) when compared with commercial ELISA kits, confirming the method's suitability for high-throughput screening and precise quantification of streptomycin residues in complex matrices such as infant formula. This provides new technical support for food safety monitoring.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IEEE SENSORS JOURNAL
·
2020
·Vol. 21
·期 5
·6300-6305
·
IF 3.073
Yujing Liu,Juan Yu,Fan Zhang,Pingang He
An all-solid-state potentiometric aptasensor based on signal amplification by polycation has been proposed for the detection of streptomycin. The carboxylated multi-walled carbon nanotubes (CMWCNTs), polyethylenimine (PEI), and the aptamers of streptomycin were assembled successively on the screen-printing carbon electrode surface by Layer-by-Layer (LbL) technique. The streptomycin would specifically bind to the aptamers, leading to the release of aptamers from the modified electrode surface. Thus, the charges of electrode were changed, causing the variation of open circuit potential (OCP). The introduction of polycation - PEI makes the electrode exposing a large amount of positive charges, so it can combine more aptamers to realize signal amplification. Consequently, under the optimal conditions, this aptasensor showed high sensitivity with corresponding linear response ranges of 1.00 χ 10_11 _ 1.00 χ 10_9 M, and a detection limit of 3.43 χ 10_12 M. Moreover, this aptasensor presented good selectivity, reproducibilityand stability. It could be applied to the detection of milk samples, demonstrating the practicability of this aptasensor.
研究领域:
分析化学
·
生物传感器
·
电化学
·
食品安全
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Shaochi Wang,Ting Du,Ying Zhu,Sijie Liu,Junqi Huangmin,Liang Zhang,Daize Shi,Minghui Zhang,Jing Sun,Daohong Zhang,Jianlong Wang
Immunochromatographic assays (ICAs) are famous as the point of care test (POCT) technology against food fraud for antibiotics detection. However, the poor sensitivity, stability and the monoclonal antibodies (mAbs)-depended signals limited their further applications. Here, inspired by the vivid color display and functionalization of natural pigments, we developed natural edible pigment-derived needle microstructure (NNMS) as signal tracers by simple crosslinking of alizarin and formaldehyde, allowing high color rendering ability, significant water dispersibility and antibody enrichment. Particularly, immune-network technology was introduced to achieve the simultaneous promotion of colorimetric signal and sensitivity and avoid visual errors in ICA. As a result, the prepared eco-friendly and sustainable NNMS-ICA showed satisfactory performance (LOD was 0.56 ng mL −1 ), specificity and probe stability (CV was 1.90 %). Furthermore, the NNMS-ICA was successfully applied in food samples (milk and honey) with total recoveries at 91.00–118.82 %.
研究领域:
食品科学
·
生物技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 12
·2868-2876
·
IF 3.641
Al Fatease Adel,Yin Junling,Guo Wenjuan,Umar Ahmad
A novel electrochemical aptasensor modified with the nanocomposite of porous carbon nanospheres and Au urchins as the signal amplification and immobility platforms for aptamer was successfully constructed for ultrasensitive and selective determination of streptomycin. The streptomycin
aptamer was fixed on the surface of the nanocomposite via the strong Au–S bond between Au urchins and aptamer. The target binding-induced conformational change of aptamer resulted in signal attenuation, which was expressed as “ΔI = I
BSA − I
streptomycin.”
Based on the synergic signal amplification platform, the as-prepared aptamer-based sensor showed a wider linearity to streptomycin from 0.01 to 350 ng/mL with a low detection limit of 5.0 pg/mL under the optimized condition. Finally, the aptasensor was operated in milk and honey to detect
streptomycin. This study has provided a facile way to develop highly sensitive, effective and efficient aptamer-based electrochemical sensors for the detection of antibiotics at very low concentration.
研究领域:
生物传感技术
·
环境科学
·
食品安全检测
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2019
·
IF 8.456
Xiaoyan He,Huimin Han,Liqin Liu,Wenyu Shi,Xiong Lu,Jiandi Dong,Wu Yang,Xiaoquan Lu
In terms of detection of antibiotics within complex media, the nonspecific adsorption is an enormous challenge and antifouling sensing interfaces capable of reducing the nonspecific adsorption from complex biological samples are highly desirable. In this work, a novel antifouling electrochemical immunosensor was explored based on the self-assembly of two kinds of poly(N-isopropylacrylamide) microgels on the surface of graphene oxide for sensitive detection of streptomycin (STR). The microgels modified with glycidyl methacrylate (GMA) and zwitterionic liquid 1-propyl-3-vinylimidazole sulfonate (PVIS) were prepared. The microgels with GMA were used by combining specific recognition of anti-STR. The rapid specific binding of antigen and anti-STR resulted in a decrease of current density to generate electrochemical responsive signals. Zwitterionic liquid-modified microgels were used for antifouling, which can form stronger hydration and show excellent antifouling ability. As a result, we achieved efficient and sensitive detection of STR in the complex sample with evidently resisted nonspecific adsorption effect, the wide linear range toward STR was from 0.05 to 100 ng mL–1, with a detection limit down to 1.7 pg mL–1. The immunosensor based on the surface functionalization of microgels showed promising applications for the detection of antibiotics in complex media.
研究领域:
分析化学
·
材料科学
·
生物化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
GLYCOBIOLOGY
·
2017
·Vol. 27
·期 9
·861-867
·
IF 3.112
Qiu Yuanhao,Hou Yilin,Sun Feifei,Chen Peng,Wang Dongdong,Mu Haibo,Zhang Xiaoli,Ding Kan,Duan Jinyou
Antibiotics such as β-lactams and aminoglycosides are often subtherapeutic to intracellular infections due to their high hydrophilicity, resulting in low effectiveness against intracellular pathogens and the emergence of antibiotic resistance. Here we reported that an endogenous aminoglycan, hyaluronic acid could be an effective carbohydrate carrier of the aminoglycoside antibiotic, streptomycin against intracellular pathogens. This conjugation could enhance phagocytic activity, and facilitated the entry of streptomycin into host cells via a CD44-mediated pathway. It appeared that this conjugate could clear intracellular bacteria in phagocytic or nonphagocytic cells in a short-term therapy (4 h) at a lower effective dose. In addition, this conjugate was more efficient in reducing bacteria burden in an in vivo acute infection model than streptomycin did. Interestingly, subcutaneous injection of this conjugate at an excess amount had undetectable side effects such as nephrotoxicity. These results suggested that hyaluronic acid might be an efficient Trojan horse for the delivery of hydrophilic antibiotics to deal with intracellular infections.
研究领域:
药理学
·
微生物学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）