SEPARATION AND PURIFICATION TECHNOLOGY
·
2024
·
IF 8.1
Sheng Wang,Xin Liang,Tengfei Lu,Ying Ma,Ziteng Wang,Xiangyu Zheng,Kaiyu Ruan,Tong Wang
Finding sustainable and efficient ways to extract natural products is becoming increasingly important for both the pharmaceutical and food industries. In this study, we propose a target-oriented approach for the rational design of composite bio-derived solvents, specifically tailored for the simultaneous extraction of salvianolic acid B, tanshinone I, cryptotanshinone and tanshinone IIA from Salvia miltiorrhiza root. We systematically evaluated the extraction efficiency of various bio-derived solvents, including glycerol carbonate, ethylene glycol, levulinic acid, glycerolformal, ethyl lactate and γ-valerolactone, both individually and in combination. Using a mixture design integrated with statistical modeling and optimization algorithms, we identified that the composite solvent system of GF/LA (57.8/42.2, v/v) greatly improved the extraction of both hydrophilic and lipophilic compounds. The extraction yields were significantly higher compared to those obtained using individual bio-derived or traditional solvents, while the overall solvent consumption was lower. Additionally, we explored the use of macroporous adsorption resins to recover the extracted compounds from GF/LA (57.8/42.2, v/v) extracts solution, achieving impressive recovery yields of over 80%. Our findings provide valuable insights into the sustainable extraction of bioactive compounds and suggest practical applications for both analytical and industrial-scale processes, aligning with green chemistry principles.
研究领域:
药用植物学
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天然产物化学
·
绿色化学
✓ 已验证
产品关联
DOI
高影响因子
PHARMACEUTICAL BIOLOGY
·
2017
·
IF 1.916
Xu-Xin Zhang,Yun-Feng Cao,Li-Xuan Wang,Xiao-Lin Yuan,Zhong-Ze Fang
Contents: Danshen is a popular herb employed to treat cardiovascular and cerebrovascular diseases worldwide. Danshen–drug interaction has not been well studied.Objective: The inhibitory effects of four major tanshinones (tanshinone I, tanshinone IIA, cryptotanshinone, and dihydrotanshinone I) on UDP-glucuronosyltransferases (UGTs) isoforms were determined to better understand the mechanism of danshen–prescription drugs interaction.Materials and methods: In vitro recombinant UGTs-catalyzed 4-methylumbelliferone (4-MU) glucuronidation reaction was employed. Tanshinones (100 μM) was used to perform the initial screening of inhibition capability. High-performance liquid chromatography (HPLC) was used to separate 4-MU and its glucuronide. In vitro–in vivo extrapolation (IV–IVE) was employed to predict in vivo inhibition situation.Results: Cryptotanshinone inhibited UGT1A7 and UGT1A9 with IC50 values of 1.91 ± 0.27 and 0.27 ± 0.03 μM, respectively. Dihydrotanshinone I inhibited UGT1A9-catalyzed 4-MU glucuronidation reaction with the IC50 value of 0.72 ± 0.04 μM. The inhibition of cryptotanshinone towards UGT1A7 and UGT1A9 was best fit to competitive inhibition type, and UGT1A9 was non-competitively inhibited by dihydrotanshinone I. Using in vitro inhibition kinetic parameters (Ki) and in vivo maximum plasma concentration (Cmax) of cryptotanshinone and dihydrotanshinone I, the change of area-under-the-concentration–time curve (AUC) was predicted to be 0.4–4.2%, 3.7–56.3%, and 0.6–6.4% induced by cryptotanshinone and dihydrotanshinone inhibition towards UGT1A7 and UGT1A9, respectively.Discussion and conclusion: The inhibitory effects of tanshinones towards important UGT isoforms were evaluated in the present study, which provide helpful information for exploring the mechanism of danshen–clinical drugs interaction.
研究领域:
药理学
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中药学
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代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2021
·
IF 5.811
Chen Tingting,Zhu Zhaoming,Du Qunqun,Wang Zhuxian,Wu Wenfeng,Xue Yaqi,Wang Yuan,Wu Yufan,Zeng Quanfu,Jiang Cuiping,Shen Chunyan,Liu Li,Zhu Hongxia,Liu Qiang
Tanshinone (TAN), a class of bioactive components in traditional Chinese medicinal plant Salvia miltiorrhiza, has antibacterial and anti-inflammatory effects, can enhance blood circulation, remove blood stasis, and promote wound healing. For these reasons it has been developed as a drug to treat acne. The purpose of this study was to evaluate the therapeutic effects of TAN in rats with oleic acid-induced acne and to explore its possible mechanisms of action through the identification of potential lipid biomarkers. In this study, a rat model of acne was established by applying 0.5 ml of 80% oleic acid to rats’ back skin. The potential metabolites and targets involved in the anti-acne effects of TAN were predicted using lipidomics. The results indicate that TAN has therapeutic efficacy for acne, as supported by the results of the histological analyses and biochemical index assays for interleukin (IL)-8, IL-6, IL-β and tumor necrosis factor alpha. The orthogonal projection of latent structure discriminant analysis score was used to analyze the lipidomic profiles between control and acne rats. Ninety-six potential biomarkers were identified in the skin samples of the acne rats. These biomarkers were mainly related to glycerophospholipid and sphingolipid metabolism, and the regulation of their dysfunction is thought to be a possible therapeutic mechanism of action of TAN on acne.
研究领域:
药理学
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皮肤病学
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传统中医药学
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产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2015
·Vol. 33
·期 9
·1084-1088
·
IF 1.578
Yafang Tan,Xiaohui Sun,Fuyue Dong,Haiyan Tian,Renwang Jiang
To enhance the structural diversity of tanshinones and provide more derivatives for the biological studies, a one-pot combinatorial modification strategy was performed on total tanshinones. Six new quinoxlinetanshinones 1 – 6 were subsequently isolated from the combinatorial modified semi-synthetic mixture. The structures were elucidated by spectroscopic analysis in combination with single-crystal X-ray diffraction. These quinoxlinetanshinones demonstrated strong DNA binding properties. Rapid synthesis of new quinoxlinetanshinones with significant biological activity highlights the great potential of one-pot combinatorial modification for the diversification of natural products.
研究领域:
药理学
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有机化学
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天然产物研究
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2018
·
IF 4.946
Qiang Huang,Meihong Sun,Tingpan Yuan,Yu Wang,Min Shi,Sunjie Lu,Boping Tang,Jingxian Pan,Yao Wang,Guoyin Kai
Tanshinones and phenolic acids are two important metabolites synthesized by the traditional Chinese medicinal plant Salvia miltiorrhiza . There is increasing market demand for these compounds. Here, we isolated and functionally characterized SmERF1L1 , a novel JA (Jasmonic acid)-responsive gene encoding AP2/ERF transcription factor, from Salvia miltiorrhiza . SmERF1L1 was responsive to methyl jasmonate (MJ), yeast extraction (YE), salicylic acid (SA) and ethylene treatments. Subcellular localization assay indicated that SmERF1L1 located in the nucleus. Overexpression of SmERF1L1 significantly increased tanshinones production in transgenic S. miltiorrhiza hairy roots by comprehensively upregulating tanshinone biosynthetic pathway genes, especially SmDXR . Yeast one-hybrid (Y1H) and electrophoretic mobility shift assay (EMSA) showed that SmERF1L1 binds to the GCC-box of SmDXR promoter while dual luciferase (Dual-LUC) assay showed that SmERF1L1 positively regulated the expression of SmDXR . Our study suggested that the SmERF1L1 may be a good potential target for further metabolic engineering of bioactive component biosynthesis in S. miltiorrhiza .
研究领域:
植物学
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药用植物学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Hao Xu,Ning Ning Guo,Chen Ying Zhu,Lin Yan Ye,Xing Yi Yan,Yong Qin Liu,Ze Yan Zhang,Guangji Zhang,Liaqat Hussain
Diterpenoid tanshinones (DTs) are a bioactive fraction extracted from Salvia miltiorrhiza. High-performance liquid chromatography analysis revealed the presence of four compounds, namely, tanshinone IIA, tanshinone I, cryptotanshinone, and dihydrotanshinone. In this study, we aimed to propose a possible mechanism for the anti-lung cancer effect of DT. To do so, we utilized a lung cancer nude mice model and a lung cancer cell line (PC9) to investigate the effect of DT on lung cancer. We employed immunohistochemistry, enzyme-linked immunosorbent assay, hematoxylin and eosin staining, and immunofluorescence to analyze the pharmacological role of DT in the inhibition of lung cancer growth. The results showed that DT inhibited tumor growth, induced apoptosis in the nude mice model, and reduced inflammatory cell infiltration. Additionally, DT inhibited PC9 lung cancer cells, growth, proliferation, and migration. The mechanism of action of DT involves not only directly inhibiting cell proliferation and migration but also improving the tumor microenvironment. DT significantly increased the expression of important intestinal gap junction proteins, such as zonula occludens 1 (ZO-1) and occludin I. This upregulation contributes to the reinforcement of the intestinal mucosal barrier, thereby reducing the paracellular transport of lipopolysaccharides (LPS) through the intestine. Consequently, the decreased LPS levels lead to the inhibition of NF-κB expression and downregulation of macrophage polarization, as indicated by the decreased expression of CD68. In conclusion, this study has confirmed that DT has anti-lung cancer properties by improving the inflammatory tumor microenvironment via regulating macrophage polarization and inhibiting LPS-associated immune response. These results provide new insights into the mechanism of DT action against lung cancer.
研究领域:
肿瘤学
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药理学
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免疫学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF EXPERIMENTAL BOTANY
·
2020
·Vol. 71
·期 19
·5948-5962
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IF 5.908
Deng Changping,Shi Min,Fu Rong,Zhang Yi,Wang Qiang,Zhou Yang,Wang Yao,Ma Xingyuan,Kai Guoyin
Phenolic acids and tanshinones are major bioactive ingredients in Salvia miltiorrhiza, which possess pharmacological activities with great market demand. However, transcriptional regulation of phenolic acid and tanshinone biosynthesis remains poorly understood. Here, a basic leucine zipper transcription factor (TF) named SmbZIP1 was screened from the abscisic acid (ABA)-induced transcriptome library. Overexpression of SmbZIP1 positively promoted phenolic acid biosynthesis by enhancing expression of biosynthetic genes such as cinnamate-4-hydroxylase (C4H1). Furthermore, biochemical experiments revealed that SmbZIP1 bound the G-Box-like1 element in the promoter of the C4H1 gene. Meanwhile, SmbZIP1 inhibited accumulation of tanshinones mainly by suppressing the expression of biosynthetic genes including geranylgeranyl diphosphate synthase (GGPPS) which was confirmed as a target gene by in vitro and in vivo experiments. In contrast, the phenolic acid content was reduced and tanshinone was enhanced in CRISPR/Cas9 [clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9]-mediated knockout lines. In addition, the previously reported positive regulator of tanshinone biosynthesis, SmERF1L1, was found to be inhibited in SmbZIP1 overexpression lines indicated by RNA sequencing, and was proven to be the target of SmbZIP1. In summary, this work uncovers a novel regulator and deepens our understanding of the transcriptional and regulatory mechanisms of phenolic acid and tanshinone biosynthesis, and also sheds new light on metabolic engineering in S. miltiorrhiza.
研究领域:
植物生物学
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分子生物学
·
药理学
·
基因工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2016
·Vol. 54
·期 8
·1435-1444
·
IF 1.32
Cao Jiliang,Wei Jinchao,Xiang Cheng,Zhang Mi,Li Baocai,Wan Jianbo,Su Huanxing,Li Peng
A simple and fast micellar electrokinetic chromatography (MEKC) method using ionic liquids as modifier was established for simultaneous determination of four hydrophobic tanshinones, including dihydrotanshinone I (1), cryptotanshinone (2), tanshinone I (3) and tanshinone IIA (4), in Danshen and related medicinal plants. In normal MEKC using sodium dodecyl sulfate (SDS) as surfactant and organic solvents as additives, the four tanshinones, especially cryptotanshinone and tanshinone I, could not be well separated. Fortunately, further addition of ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim]BF4) resulted in a baseline separation of these four analytes. After an optimization study, 10 mM borax–10 mM SDS–10 mM [bmim]BF4 containing 15% acetonitrile (v/v) at pH 9.6 was adopted as the running buffer to complete the separation within 16 min at the voltage of 25 kV, temperature of 25°C and detection wavelength of 254 nm. The relative standard deviations of migration time and peak area were in the range of 0.62–2.21 and 1.33–3.90%, respectively, indicating the good repeatability of the developed method. This method was extensively validated by evaluating the linearity (R2 ≥ 0.9992), limits of detection (0.75–1.11 μg mL−1), limits of quantification (2.26–3.32 μg mL−1) and recovery (96.11–103.74%). Under the optimum conditions, samples of Danshen and related medicinal plants were well analyzed with high separation efficiency.
研究领域:
分析化学
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药物化学
·
药用植物
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）