A monoclonal antibody (mAb) was raised against tebuconazole (TEB) using a hapten where the p-chloro substituent of the TEB molecule was replaced with a long-chain carboxylic acid. The resulting mAb showed high sensitivity and specificity against TEB characterized by ELISA with a half-maximal inhibitory concentration (IC50) of 0.19 ng mL–1 and with cross-reactivity (CR) values below 0.01% to several analogues of triazole fungicides. On the basis of the mAb produced, a quantum dot beads-based fluorescence immunochromatographic test strip assay (QBs–FITSA) was developed for rapid and sensitive detection of TEB in agricultural product samples. The QBs–FITSA exhibited a linear detection range from 0.02 to 1.25 ng mL–1 with a limit of detection (LOD) of 0.02 ng mL–1. Furthermore, using produced mAb, multiple high-throughput rapid immunoassay formats could be achieved as a convenient monitoring tool for evaluation of human and environmental exposure to TEB.
Conventional surface plasmon resonance (SPR) sensor often fails to detect low-concentration small molecules due to insufficient refractive index changes. To address this challenge, we have developed an enhanced SPR sensor utilizing Fe 3 O 4 @Au@polydopamine (PDA) core–shell magnetic nanoparticles as signal amplification tags for the detection of tebuconazole (TBZ). These nanoparticles improve sensitivity through their high refractive indices and enable TBZ enrichment from complex matrices via their magnetic properties. The sensor surface is further modified with PDA, which acts as a reducing agent for Au nanoparticle deposition, enhancing monoclonal antibody (Ab) immobilization and boosting the SPR signal. Our optimized sensor reaches a limit of detection (LOD) at 0.61 ng/mL for TBZ, with a linear response from 1-200 ng/mL, and demonstrates high selectivity and reproducibility, ideal for pesticide monitoring in food samples.
危险品化学品经营许可证（带存储）