ANALYST
·
2023
·Vol. 148
·期 4
·912-918
·
IF 4.2
Xinai Zhang,Zhenzhong Wang,Xu Li,Wei Xiao,Xiaobo Zou,Qilin Huang,Lili Zhou
The development of effective methods for tracking cancer cells is of significant importance in the early diagnosis and treatment of tumor diseases. Compared with the developed techniques, the electrochemical assay has shown considerable potential for monitoring glycan expression on the cell surface using nondestructive means. However, the application expansion of the electrochemical strategy is strongly impeded owing to its dependence on electroactive species. In this study, a competitive electrochemical strategy was reported for monitoring cancer cells based on mannose (a typical glycan) as a clinical biomarker. Herein, functionalized carbon nanotubes were used to load the thiomannosyl dimer, and thionine-interlinking signal probes were designed for competitive recognition. After effective competition between cancer cells and the anchored mannose, a decreased current was obtained as the cell concentration increased. Under optimal conditions, the proposed biosensor exhibited attractive performance for cancer cell analysis with a detection limit as low as 20 cells per mL for QGY-7701 and 35 cells per mL for QGY-7703, facilitating great promise for the sensitive detection of cancer cells and thus showing potential applications in cancer diagnosis.
研究领域:
生物医学工程
·
肿瘤学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2012
·
IF 5.602
Limei Zhu,Liqiang Luo,Zhenxin Wang
A novel protocol for development of DNA electrochemical biosensor based on thionine-graphene nanocomposite modified gold electrode was presented. The thionine-graphene nanocomposite layer with highly conductive property was characterized by scanning electron microscopy, transmission electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. An amino-substituted oligonucleotide probe was covalently grafted onto the surface of the thionine-graphene nanocomposite by the cross-linker glutaraldehyde. The hybridization reaction on the modified electrode was monitored by differential pulse voltammetry analysis using an electroactive intercalator daunomycin as the indicator. Under optimum conditions, the proposed biosensor exhibited high sensitivity and low detection limit for detecting complementary oligonucleotide. The complementary oligonucleotide could be quantified in a wide range of 1.0 × 10 −12 to 1.0 × 10 −7 M with a good linearity ( R 2 = 0.9976) and a low detection limit of 1.26 × 10 −13 M (S/N = 3). In addition, the biosensor was highly selective to discriminate one-base or two-base mismatched sequences.
研究领域:
生物化学
·
纳米技术
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 18
·5365-5370
·
IF 3.969
Zhao Zhang,Liqiang Luo,Limei Zhu,Yaping Ding,Dongmei Deng,Zhenxin Wang
A novel protocol of electrochemical thrombin aptasensor based on a goldnanoparticles/thionine–graphene (AuNPs/Th–G) nanocomposite modified glassy carbon electrode was presented. Graphene was non-covalently functionalized by thionine via π-stacking interaction, and then AuNPs were electrodeposited onto the Th–G surface. The morphology and conductivity of the AuNPs/Th–G nanocomposite were characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. The ethanethiol-substituted oligonucleotide probe was immobilized onto the surface of the AuNPs/Th–G nanocomposite to prepare an electrochemical biosensing platform. The aptamer–thrombin reaction was monitored by differential pulse voltammetry. Under optimum conditions, the proposed biosensor exhibits high sensitivity and a low detection limit for thrombin determination. The thrombin could be quantified in a wide range of 0.5 to 40 nM and with a low detection limit of 0.093 nM (S/N = 3).
研究领域:
生物化学
·
纳米技术
·
分析化学
·
电化学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Li Wang,Longfei Miao,Han Yang,Jie Yu,Yingzhen Xie,Lijuan Xu,Yonghai Song
Here, a new nanoenzyme of Fe 3 O 4 nanoparticles (NPs) magnetic molecularly imprinted polymers (MMIPs) was prepared by polymerizing dopamine on the Fe 3 O 4 NPs surface in the presence of templated thionine (Thi) for the first time. The results showed that uniform spherical and core-shell structured Fe 3 O 4 NPs MMIPs which were about 600 nm in diameter were successfully formed and the imprinting sites improved the selectivity of Fe 3 O 4 NPs MMIPs greatly. The as-prepared Fe 3 O 4 NPs MMIPs could catalyze the reduction of Thi selectively, which could be enhanced by H 2 O 2 owing to the peroxidase-like activity of Fe 3 O 4 NPs. Accordingly, a highly selective and sensitive H 2 O 2 electrochemical biosensor was proposed based on the Fe 3 O 4 NPs MMIPs-modified glassy carbon electrode. The electrochemical biosensor based on the Fe 3 O 4 NPs MMIPs nanoenzyme exhibited low detection limit of 1.58 nM and high selectivity. Since acetylthiocholine chloride (AChl) could be hydrolyzed into choline with the help of acetylcholinesterase (AChE) and simultaneously the choline oxidase (ChOx) could reduce choline into betaine accompanied by the production of H 2 O 2 , the proposed electrochemical H 2 O 2 biosensor could be further used to detect AChl, AChE and ChOx. The results also exhibited wide linear range (2.85–160 μM for AChl, 0.53–20000 ng mL −1 for AChE and 22.76–400 ng mL −1 for ChOx), low detection limit (0.86 μM for AChl, 0.16 ng mL −1 for AChE and 6.83 ng mL −1 for ChOx) and high selectivity. Therefore, the Fe 3 O 4 NPs MMIPs should be a promising nanoenzyme for electrochemical biosensors.
研究领域:
生物化学
·
纳米生物学
·
分析化学
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产品关联
DOI
高影响因子
ANALYST
·
2015
·Vol. 140
·期 16
·5570-5577
·
IF 4.107
Zhen Zhao,Hongda Chen,Lina Ma,Dianjun Liu,Zhenxin Wang
It is important to develop methods to determine cylindrospermopsin (CYN) at trace levels since CYN is a kind of widespread cyanobacterial toxin in water sources. In this study, a label-free impedimetric aptasensor has been fabricated for detecting CYN. In this case, the amino-substituted aptamer of CYN was covalently grafted onto the surface of the thionine–graphene (TH–G) nanocomposite through the cross-linker glutaraldehyde (GA). The reaction of the aptamer with CYN was monitored by electrochemical impedance spectroscopy because the CYN induced conformation change of the aptamer can cause a remarkable decrease of the electron transfer resistance. Under optimum conditions, the aptasensor exhibits high sensitivity and a low detection limit for CYN determination. The CYN can be quantified in a wide range of 0.39 to 78 ng mL−1 with a good linearity (R2 = 0.9968) and a low detection limit of 0.117 ng mL−1. In addition, the proposed aptasensor displays excellent stability, reusability and reproducibility.
研究领域:
环境科学
·
分析化学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2024
·
IF 3.6
Yi Zhang,Xue Kong,Haiyang Guo,Jing Wang,Zheng-Zhi Yin
An electrochemical microsensor for mesothelin (MSLN) based on an acupuncture needle (AN) was constructed in this work. To prepare the microsensor, MSLN was self-assembled on 4-mercaptophenylboric acid (4-MPBA) by an interaction force between the external cis-diol and phenylboronic acid. This was followed by the gradual electropolymerization of thionine (TH) and eriochrome black T (EBT) around the anchored protein. The thickness of the surface imprinted layers influenced the sensing performance and needed to be smaller than the height of the anchored protein. The polymerized EBT was not electrically active, but the polymerized TH provided a significant electrochemical signal. Therefore, electron transfer smoothly proceeded through the eluted nanocavities. The imprinted nanocavities were highly selective toward MSLN, and the rebinding of insulating proteins reduced the electrochemical signal of the embedded pTH. The functionalized interface was characterized by SEM and electrochemical methods, and the preparation conditions were studied. After optimization, the sensor showed a linear response in the range of 0.1 to 1000 ng/mL with a detection limit of 10 pg/mL, indicating good performance compared with other reported methods. This microsensor also showed high specificity and stability, which can be attributed to the fine complementation of the imprinted organic nanocavities. The sensitivity of this sensor was related to the nanocavities used for electron transport around the AuNPs. In the future, microsensors that can directly provide electrochemical signals are expected to play important roles especially on AN matrices.
研究领域:
生物化学
·
电化学
·
传感器
·
纳米技术
·
医疗器械
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Qingqing Hao,Lilin Lu,Xianwen Kan
Molecular imprinted polymer (MIP) has been employed for electrochemical sensor preparation with good selectivity. However, to restrict the non-specific adsorption, the conductivity of MIP is usually unsatisfying, which limits its sensitivity for template detection. To achieve both of selectivity and sensitivity, a MIP electrochemical sensor was designed and fabricated by the electropolymerization of pyrrole in the presence of aloe-emodin (AE, template molecule) on carbon nanoparticles modified electrode in this work. Thionine (TH), being added in solution in advance, played double roles for AE recognition and detection. As an analogue of AE, TH was adsorbed into imprinted cavities and then was replaced by AE, indicating the good selectivity of the sensor. This replacement of TH by AE was also demonstrated by the result of computational simulation. As an electro-active probe, the peak current of TH decreased and peak current of AE increased based on the replacement of TH by AE. A current ratiometric signal defined as |Δ i AE |/|Δ i TH | was used for AE sensitive detection. Thus, the replacements of TH by AE demonstrated that the sensor possessed high sensitivity and good selectivity. A linear range of 1.0 × 10 −7 -1.0 × 10 -4 mol/L for AE detection was obtained with a limit of detection of 7.5 × 10 -8 mol/L. And the sensor has been applied to analyze AE in real samples with satisfactory results. The simple, smart, and effective strategy presented in this work can be further used to prepare electrochemical sensors for other compounds selective recognition and sensitive detection.
研究领域:
分析化学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Dan Tao,Chang Xie,Sinan Fu,Shuang Rong,Shizhen Song,Huarong Ye,Nicole Jaffrezic-Renault,Zhenzhong Guo
How to sensitively detect early biomarkers of Alzheimer's disease (AD) is nowadays, one of the major challenges. In this work, Aβ oligomers (AβO), one of the AD biomarkers, was analyzed using an electrochemical aptasensor , which was prepared based on thionine (Th) - functionalized three - dimensional carbon nanomaterials (reduced graphene oxide (rGO) and multi-wall carbon nanotubes (MWCNTs)) immobilized DNA-aptamer. Th, a positively charged planar aromatic molecule, form many π - π conjugated structures with rGO and MWCNTs, then improving the structural stability, electron transfer and the capacitive properties of Th-rGO-MWCNTs nanocomposites. Under the optimal conditions, differential pulse voltammetry (DPV) current responses decreased with the increase of AβO concentration. The obtained AβO aptasensor presented a wide linear range of 0.0443 pM–443.00 pM and limit of detection (LOD) was 10 fM. Meanwhile, AβO aptasensor displayed remarkable stability and selectivity . It has a great potential for early diagnosis of AD in human real serum samples.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sensors & Diagnostics
·
2025
·
IF 4.1
Yaping Zhang,Gao Si,Zhendong Wang,Yilong Wang,Xiaojing Cui,Huaixia Yang,Fuchun Si,Yanjiu Liu
The analysis of down-regulator of transcription 1 (DR1) offers significant information for the rapid and non-invasive diagnosis of Hashimoto's thyroiditis (HT). In this study, we report a novel dual-signal amplification electrochemical biosensor for the sensitive detection of DR1. Gold nanoparticle (AuNP)-modified molybdenum disulfide (MoS2@AuNPs), which has extremely strong electron transfer ability and abundant binding sites, is first modified on an electrode surface as a substrate material to implement the first signal amplification. After the formation of the sandwich structure based on the specific recognition of antigens and antibodies, the electroactive molecules hyaluronic acid-based thionine (HA@Thi) are introduced to achieve the second signal amplification. Using this dual-signal amplification strategy, the proposed biosensor achieves a linear range of 1 × 10−4–1 × 102 ng mL−1 with a low detection limit of 10.99 fg mL−1. In addition, the electrochemical biosensor has high selectivity and good stability, and is applicable to the assay of DR1 in the presence of complex biological matrices, which is expected to provide a scientific approach for the clinical application of serum DR1 monitoring. More importantly, our method may extend the application of protein-based biosensors in disease diagnosis techniques.
研究领域:
医学诊断
·
生物传感技术
·
纳米材料应用
✓ 已验证
产品关联
DOI
高影响因子
Nanoscience and Nanotechnology Letters
·
2018
·Vol. 10
·期 4
·520-527
·
IF 2.917
Deng Yan,Du Xueting,Ma Yuchan,Zhang Kunlei,Zhang Xi,Li De-Lei,Bai Ruyan,Hu Rong,Yang Yun-Hui
In this paper, an ultrasensitive electrochemical immunoassay based on porous covalent organic framework materials and Au NPs-CoS/graphene oxide (GO) nanocomposite was developed for the detection of C-reactive protein (CRP). Au NPs-CoS/graphene oxide (GO) nanocomposite was synthesized,
and employed as substrate because of their high conductivity to immobilize CRP antibody. A new porous covalent organic framework material (COF-LZU8) was functionalized by gold nanoparticles and doped with thionine (Thi) as the electron transfer mediator to obtain nanocomposite. The reduction
peak signal of Thi/Au NPs-COF-LZU8 at –0.2 V could be directly detected by highly sensitive differential pulse voltammetry (DPV). Due to the high surface area of COF-LZU8, a great quantity of Thi could be adsorbed on the surface of COF, thus, the signal could be greatly amplified. Moreover,
Thi/Au NPs-COF-LZU8 has strong adsorption capacity to the anti-CRP protein macromolecules due to the large specific surface area and good bio-affinity. The concentration of CRP antigen was proportional to the response current, so the quantitative detection of CRP was realized. Under the optimum
conditions, the linear range of CRP was 0.05–150 ng/mL with the detection limit of 0.016 ng/mL (S/N = 3). This immunosensor provides a more effective and sensitive method for detection of CRP.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 2
·1-8
·
IF 5.479
He Baoshan,Yan Sasa
A signal-on aptasensor is described for the voltammetric determination of kanamycin (KANA). Au@Pt core-shell nanoparticles with large surface and good electrical conductivity were synthetized and act as both a conductive material and as the carrier for complementary strands (CS2) and thionine (TH). In the presence of KANA, the electrochemical response of TH changes due to hybridization between CS1 immobilized on the electrode and the Au@Pt-CS2/TH system. The peak current increases linearly with the logarithm of the KANA concentration in the range from 1 pM to 1 μM, and the limit of detection is 0.16 pM. The sensor was characterized in terms of selectivity, reproducibility and stability, and satisfactory results were obtained. It was also utilized for the determination of KANA in (spiked) chicken samples. The recoveries (95.8–103.2%) demonstrate the potential of the method for KANA detection in real samples.
A signal-on aptasensor for kanamycin (KANA) was developed by using Au@Pt core-shell nanoparticles as nanocarrier for probe aptamer and as a sensing probe.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
电化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2022
·
IF 5.518
Xiaoli Liu,Hongxia Zhang,Junping Ma,Jianbin Zheng
A novel non-enzymatic nitrite (NO 2 − ) electrochemical sensor was fabricated based on Au-poly(thionine) and tin oxide/graphene nanosheets (Au-PTH/SGN) nanocomposites . The morphology, composition and structure of the nanocomposites were characterized by Transmission electron microscopy, Energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy , X-ray diffraction and X-ray photoelectron spectroscopy. And the results indicated that homogeneous Au NPs with a size of approximate 80 nm and network nanostructure of PTH were attached on the surface of SGN nanomaterials. It had been also verified to satisfactory electrochemical results. The linear range of the proposed sensor was five orders of magnitude wide with the range of 0.2–127.7 (R 2 = 0.9991) and 127.7–2800 (R 2 = 0.9983) μmol L −1 , the detection limit was determined to be 0.06 μmol L −1 (S/N＝3) and the sensitivity were 61.92 and 43.19 μA (mmol L −1 ) −1 cm −2 , respectively. Compared with the similar type of NO 2 − sensors previously reported, the linear range was expanded by at least an order of magnitude and the detection limit was kept at the same level as the lowest. Furthermore, the constructed Au-PTH/SGN/GCE displayed fast response, excellent stability, reproducibility, and high anti-interference ability for NO 2 − detection. Moreover, the as-proposed electrode was successfully applied for detecting NO 2 − in tap water and sewage samples with recoveries ranging from 97.3% to 103.0%97.3%-103.0% and 98.7–102.6%, respectively. The present work is expected to provide a useful route and reference for designing novel high performance electrochemical sensors and broaden the applications of sensors .
研究领域:
分析化学
·
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）