INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2016
·
IF 3.138
Yin Wan,Bin Zou,Hailong Zeng,Lunning Zhang,Ming Chen,Guiming Fu
In this study, we analyzed the inhibitory effect of verbascoside against xanthine oxidase (XOD) in vitro by using animal model and in vivo by direct inhibition assay. Results showed that verbascoside could reduce uric acid in rat serum and inhibit XOD activity in rat liver. The IC 50 value of restraining XOD activity was 81.11 mg mL −1 . Fluorescence chromatographic analysis and circular dichroism spectroscopy indicated that the secondary structures of XOD were changed after incubation with verbascoside. The docking simulation showed that verbascoside could enter into the active site of XOD and form hydrogen bonding with amino acid residues (such as Lys-1045, Arg-880, Arg-912, Glu-1261 and Gln-1194). The results suggested that verbascoside, which is a naturally occurring water-soluble antioxidant, could be a potential low-toxicity XOD inhibitor for hyperuricemia treatment.
研究领域:
药理学
·
生物化学
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DOI
PubMed
高影响因子
Scientific Reports
·
2025
·Vol. 15
·期 1
·1-14
·
IF 3.9
Zhang Yuxi,Zhan Junbiao,Qiu Zhen,Tian Hao,Lei Shaoqing,Huang Qin,Xue Rui,Sun Qian,Xia Zhongyuan
Myocardial cold ischemia/reperfusion (I/R) injury is an inevitable consequence of heart transplantation, significantly affecting survival rates and therapeutic outcomes. Growth Differentiation Factor 15 (GDF15) has been shown to regulate GPX4-mediated ferroptosis, playing a critical role in mitigating I/R injury. Meanwhile, verbascoside (VB), an active compound extracted from the herbaceous plant, has demonstrated myocardial protective effects. In this study, heart transplantation was performed using a modified non-suture cuff technique, with VB administered at a dose of 20 mg/kg/day via intraperitoneal injection for 3 days in vivo. In vitro, cardiomyocytes were pretreated with 50 µg/ml VB for 24 h. VB treatment significantly reduced histopathological injury, decreased myocardial injury markers, and inhibited ferroptosis and oxidative stress during myocardial cold I/R injury in vivo. In vitro experiments further demonstrated that GDF15 alleviates ferroptosis induced by hypoxic reoxygenation by upregulating GPX4. Therefore, it is concluded that VB preconditioning can effectively reduce ferroptosis induced by myocardial cold I/R after heterotopic heart transplantation, possibly through up-regulation of GDF15/GPX4/SLC7A11 pathway.
研究领域:
心血管病学
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药理学
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移植学
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DOI
PubMed
高影响因子
Antioxidants
·
2024
·
IF 6
Xiao Xiao,Feiyu Yang,Yuling Huang,Shaohui Liu,Zhenhua Hu,Shanggao Liao,Yuanyuan Li
Numerous approaches targeting hepatic stellate cells (HSCs) have emerged as pivotal therapeutic strategies to mitigate liver fibrosis and are currently undergoing clinical trials. The investigation of herbal drugs or isolated natural active compounds is particularly valuable, due to their multifaceted functions and low risk of side effects. Recent studies have hinted at the potential efficacy of verbascoside (VB) in ameliorating renal and lung fibrosis, yet its impact on hepatic fibrosis remains to be elucidated. This study aims to evaluate the potential effects of VB on liver fibrosis by assessing its ability to inhibit HSC activation. VB demonstrated significant efficacy in suppressing the expression of fibrogenic genes in activated LX-2 cells. Additionally, VB inhibited the migration and proliferation of these activated HSCs by scavenging reactive oxygen species (ROS) and downregulating the AMPK pathway. Furthermore, a biosafe reverse microemulsion loaded with VB (VB-ME) was developed to improve VB’s instability and low bioavailability. The optimal formulation of VB-ME was meticulously characterized, revealing substantial enhancements in cellular uptake, ROS-scavenging capacity, and the suppression of HSC activation.
研究领域:
药理学
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肝病学
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药物递送系统
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Plant Communications
·
2023
·
IF 10.5
Yang Y,Xi D,Wu Y,Liu T
Verbascoside, which was first discovered in 1963, is a well-known phenylethanoid glycoside (PhG) that exhibits antioxidant, anti-inflammatory, antimicrobial, and neuroprotective activities and contributes to the therapeutic effects of many medicinal plants. However, the biosynthetic pathway of verbascoside remains to be fully elucidated. Here, we report the identification of two missing enzymes in the verbascoside biosynthesis pathway by transcriptome mining and in vitro enzymatic assays. Specifically, a BAHD acyltransferase (hydroxycinnamoyl-CoA:salidroside hydroxycinnamoyltransferase [SHCT]) was shown to catalyze the regioselective acylation of salidroside to form osmanthuside A, and a CYP98 hydroxylase (osmanthuside B 3,3'-hydroxylase [OBH]) was shown to catalyze meta-hydroxylations of the p-coumaroyl and tyrosol moieties of osmanthuside B to complete the biosynthesis of verbascoside. Because SHCTs and OBHs are found in many Lamiales species that produce verbascoside, this pathway may be general. The findings from the study provide novel insights into the formation of caffeoyl and hydroxytyrosol moieties in natural product biosynthetic pathways. In addition, with the newly acquired enzymes, we achieved heterologous production of osmanthuside B, verbascoside, and ligupurpuroside B in Escherichia coli; this work lays a foundation for sustainable production of verbascoside and other PhGs in micro-organisms.
研究领域:
生物化学
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植物代谢
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分子生物学
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生物技术
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DOI
PubMed
高影响因子
Nanomedicine-Nanotechnology Biology and Medicine
·
2026
·
IF 4.3
Mifang Yang,Jingjing Wu,Yuan Jing,Siyu Chen,Rongrong Cao,Heming Wu,Hongming Du,Zhirui Guo,Fan Zhang,Wei Shu,Yi Yuan
Oral squamous cell carcinoma (OSCC), the most common oral malignancy, requires innovative therapeutic strategies. We developed a magnetic nanoparticle carrying verbascoside (VB-Fe 3 O 4 ) to enhance the bioavailability and antitumor efficacy of VB, a bioactive compound from Rehmannia glutinosa. VB-Fe 3 O 4 demonstrated superior tumor-targeting capabilities, achieving higher drug accumulation compared with free VB. In the human OSCC cell line HSC-3, VB-Fe 3 O 4 markedly induced apoptosis, inhibited proliferation and invasion through IL-6/STAT3 pathway. Mechanistic studies screened out intercellular cell adhesion molecule 1 (ICAM1) as the primary target of VB, with VB-Fe 3 O 4 suppressing IL-6-induced STAT3 phosphorylation. VB-Fe 3 O 4 downregulated metastasis-related genes, such as vascular cell adhesion molecule 1, mucin 1, ezrin, and invasion factor matrix metalloproteinase-9, were markedly decreased by VB-Fe 3 O 4 . VB-Fe 3 O 4 showed greater tumor suppression in OSCC transplanted tumors and experimental metastasis tumors compared with free VB. This nanoplatform synergistically combines precision delivery and molecular targeting, offering a promising treatment for OSCC management.
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DOI
PubMed
高影响因子
Separation Science Plus
·
2026
·Vol. 9
·期 9
·e70309
·
IF 2.7
Wenbei Jin,Bo Huang,Panxu Wang,Xinsheng Wang
An efficient ultrasound-assisted aqueous two-phase extraction method was developed for the extraction of verbascoside from the leaves of Syringa pubescens Turcz. The ethanol/ammonium sulfate system was used as the extraction solvent. Based on the results of single-factor experiments, the key parameters, including ethanol concentration, ammonium sulfate concentration, and sample mass, were further optimized using response surface methodology. The optimal extraction conditions were obtained as follows: ethanol concentration of 30% (w/w), ammonium sulfate concentration of 19% (w/w), sample mass of 0.63 g, ultrasonic power of 150 W, extraction time of 30 min, and temperature of 40°C. Under these conditions, the experimental verbascoside yield (5.23 ± 0.29 mg/g) was in good agreement with the model-predicted value (5.33 mg/g), confirming the reliability of the established model. Furthermore, the verbascoside extract exhibited potent radical scavenging activities with half‑maximal inhibitory concentration values of 0.039 mg/mL against α, α-diphenyl-β-picrylhydrazyl and 0.058 mg/mL against 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid), as well as a ferric-reducing antioxidant power of 2.78 mg Fe 2 + /g at 2.5 mg/mL. This study demonstrates that the ultrasound-assisted aqueous two-phase extraction method is an efficient, green, and reliable approach for the extraction of verbascoside from S. pubescens leaves, and the obtained verbascoside extract shows promise as a natural antioxidant agent for potential applications in the food and pharmaceutical industries.
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高影响因子
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