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GCSF Receptor Antibody

功能和特点
  • 规格或纯度: pAb; Rabbit anti Human GCSF Receptor Antibody; WB, IHC, ICC/IF ; Unconjugated
  • 简短描述: Rabbit polyclonal to GCSF Receptor
  • 反应种属:
  • 亚型: Rabbit IgG
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab105080-10μl
 10μl 期货 Stock Image
Ab105080-20μl
 20μl 期货 Stock Image
Ab105080-50μl
 50μL 现货 Stock Image
Ab105080-100μl
 100μL 现货 Stock Image
Ab105080-1ml
 1ml 期货 Stock Image
大包装询价 添加到收藏夹 比较  SDS中文版  DATASHEET
main product photo

基本信息

产品名称 GCSF Receptor Antibody
别名 粒细胞-巨噬细胞集落刺激因子3受体抗体
英文别名 CD114 | G-CSFR | CSF3R | CSF3-R | GCSF-R | CD 114 antibody | CD114 antibody | CD114 antigen antibody | Colony stimulating factor 3 receptor (granulocyte) antibody | Colony stimulating factor 3 receptor antibody | CSF 3R antibody | CSF3R antibody | CSF3R_H
规格或纯度 pAb; Rabbit anti Human GCSF Receptor Antibody; WB, IHC, ICC/IF ; Unconjugated
特异性 CSF3R
免疫原 Recombinant GCSFR expressed in E.coli (AA 26-138).
阳性对照 WB: THP1, MCF7, K562, Hep G2 and U937 cell lysates. IHC: Human placenta, appendix, stomach cancer, colorectal cancer tissues. ICC/IF: MCF7 cells.
偶联标记 1

产品属性

克隆 3
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Antigen affinity purified + Protein A purified
物理外观 Liquid
储存缓冲液 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.4
防腐剂 0.02% Sodium azide
浓度 Lot by Lot
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application Notes
IHC

5-20 µg/mL
ICC/IF

5-20 µg/mL

WB

0.01-2 µg/mL

质检证书(COA)

质检报告(COA)

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图片

GCSF Receptor Antibody (Ab105080) - Western Blot
All lanes: GCSF Receptor Antibody (Ab105080) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 85 kDa
Observed band size: 78 kDa
Exposure time: 3.4 s

GCSF Receptor Antibody (Ab105080) - IF/ICC
FITC staining on IF
Sample: MCF7 cells
Primary Ab: 20 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Appendix Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Stomach cancer Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 10 μg/mL GCSF Receptor Antibody (Ab105080)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

GCSF Receptor Antibody (Ab105080) - IHC
DAB staining on IHC
Sample: Human Placenta Tissue
Primary Ab: 10 µg/mL GCSF Receptor Antibody (Ab105080)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

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参考文献

1. Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R.  (2006)  Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex..  Proc Natl Acad Sci USA,  103  (9):  (3135-40).  [PMID:16492764]
2. Seto, Y Y, Fukunaga, R R and Nagata, S S..  (1992)  Chromosomal gene organization of the human granulocyte colony-stimulating factor receptor..  Journal of immunology (Baltimore, Md. : 1950),    (1):  ().  [PMID:1530796]
3. Fukunaga, R R, Seto, Y Y, Mizushima, S S and Nagata, S S..  (1990)  Three different mRNAs encoding human granulocyte colony-stimulating factor receptor..  Proceedings of the National Academy of Sciences of the United States of America,    ():  ().  [PMID:1701053]
4. Fukunaga, R R, Ishizaka-Ikeda, E E, Pan, C X CX, Seto, Y Y and Nagata, S S..  (1991)  Functional domains of the granulocyte colony-stimulating factor receptor..  The EMBO journal,    ():  ().  [PMID:1717255]
5. Larsen, A A and 9 more authors..  (1990)  Expression cloning of a human granulocyte colony-stimulating factor receptor: a structural mosaic of hematopoietin receptor, immunoglobulin, and fibronectin domains..  The Journal of experimental medicine,    (1):  ().  [PMID:2147944]
6. Dong, F F and 7 more authors..  (1994)  Identification of a nonsense mutation in the granulocyte-colony-stimulating factor receptor in severe congenital neutropenia..  Proceedings of the National Academy of Sciences of the United States of America,    (10):  ().  [PMID:7514305]
7. Haniu, M M and 5 more authors..  (1995)  Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region..  Archives of biochemistry and biophysics,    (20):  ().  [PMID:8554326]
8. Yamasaki, K K, Naito, S S, Anaguchi, H H, Ohkubo, T T and Ota, Y Y..  (1997)  Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand..  Nature structural biology,    ():  ().  [PMID:9187659]
9. Layton, J E JE, Iaria, J J, Smith, D K DK and Treutlein, H R HR..  (1997)  Identification of a ligand-binding site on the granulocyte colony-stimulating factor receptor by molecular modeling and mutagenesis..  The Journal of biological chemistry,    (21):  ().  [PMID:9368043]
10. Ward, A C AC and 7 more authors..  (1999)  Novel point mutation in the extracellular domain of the granulocyte colony-stimulating factor (G-CSF) receptor in a case of severe congenital neutropenia hyporesponsive to G-CSF treatment..  The Journal of experimental medicine,    (16):  ().  [PMID:10449521]
11. Elsässer, Annika A and 12 more authors..  (2003)  The fusion protein AML1-ETO in acute myeloid leukemia with translocation t(8;21) induces c-jun protein expression via the proximal AP-1 site of the c-jun promoter in an indirect, JNK-dependent manner..  Oncogene,    (28):  ().  [PMID:12944913]
12. Cao, Yan-Ran YR and 11 more authors..  (2005)  [The response of bone marrow hematopoietic cells to G-CSF in paroxysmal nocturnal hemoglobinuria patients]..  Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi,    ():  ().  [PMID:15949269]
13. Zhuang, Dazhong D, Qiu, Yaling Y, Haque, S Jaharul SJ and Dong, Fan F..  (2005)  Tyrosine 729 of the G-CSF receptor controls the duration of receptor signaling: involvement of SOCS3 and SOCS1..  Journal of leukocyte biology,    ():  ().  [PMID:16033816]
14. Erkeland, S J SJ and 9 more authors..  (2007)  Novel role of WD40 and SOCS box protein-2 in steady-state distribution of granulocyte colony-stimulating factor receptor and G-CSF-controlled proliferation and differentiation signaling..  Oncogene,    (29):  ().  [PMID:17001306]
15. and Ward, Alister C AC..  (2007)  The role of the granulocyte colony-stimulating factor receptor (G-CSF-R) in disease..  Frontiers in bioscience : a journal and virtual library,    (1):  ().  [PMID:17127322]
16. Link, Daniel C DC and 28 more authors..  (2007)  Distinct patterns of mutations occurring in de novo AML versus AML arising in the setting of severe congenital neutropenia..  Blood,    (1):  ().  [PMID:17494858]
17. Zeidler, Cornelia C, Germeshausen, Manuela M, Klein, Christoph C and Welte, Karl K..  (2009)  Clinical implications of ELA2-, HAX1-, and G-CSF-receptor (CSF3R) mutations in severe congenital neutropenia..  British journal of haematology,    ():  ().  [PMID:19120359]
18. Plo, Isabelle I and 11 more authors..  (2009)  An activating mutation in the CSF3R gene induces a hereditary chronic neutrophilia..  The Journal of experimental medicine,    (3):  ().  [PMID:19620628]
19. Triot, Alexa A and 20 more authors..  (2014)  Inherited biallelic CSF3R mutations in severe congenital neutropenia..  Blood,    (12):  ().  [PMID:24753537]
20. Klimiankou, Maksim M and 8 more authors..  (2015)  GM-CSF stimulates granulopoiesis in a congenital neutropenia patient with loss-of-function biallelic heterozygous CSF3R mutations..  Blood,    (8):  ().  [PMID:26324699]
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