GFP Mouse mAb

功能和特点

规格或纯度: mAb (3A10); Mouse anti GFP Antibody; WB, IP; Unconjugated
简短描述: Mouse monoclonal (3A10) to GFP
亚型: Mouse IgG1

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab105284-10μl	10μl	期货
Ab105284-50μl	50μL	现货
Ab105284-100μl	100μL	现货
Ab105284-1ml	1ml	期货

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基本信息

产品名称	GFP Mouse mAb
别名	GFP antibody; Green fluorescent protein antibody; eGFP; Enhanced Green Fluorescent Protein; GFP; GFPuv; green fluorescent protein (gfp); Green Fluorescent Protein; GFP Tag antibody; Anti- GFP Tag antibody; GFP tag 标签单克隆抗体; GFP tag 单克隆抗体; GFP tag 抗体
规格或纯度	mAb (3A10); Mouse anti GFP Antibody; WB, IP; Unconjugated
特异性	GFP
免疫原	Synthetic peptide GFP.
阳性对照	WB: HEK293T whole cell lysate transfected with pEGFP-C3-ATG3, HEK293T whole cell lysate transfected with pEGFP-C3-ATG12. IP: HEK293T transfected with pEGFP-C3-ATG3#PPL00361-2a.
偶联标记	1

产品属性

克隆	2
克隆号	3A10
Format	Whole IgG
亚型	Mouse IgG1
SDS-PAGE	150 kDa
纯化方法	Antigen affinity purified
物理外观	Liquid
储存缓冲液	10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH 7.2
防腐剂	0.02% Sodium azide
浓度	1.0 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
WB	1/5000 – 1/10000
IF/ICC	1/100-1/500

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图片

Western Blot: GFP Mouse mAb (Ab105284)

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Lysates at 10 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 29 kDa
Exposure time: 2.3 s

Western Blot: GFP Mouse mAb (Ab105284)

GFP Mouse mAb (Ab105284) - Western Blot
All lanes: GFP Mouse mAb (Ab105284) at 1/10000 dilution
Samples: Recombinant GFP Protein (rp156636) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (138040) at 1/30000 dilution

Predicted band size: 27 kDa
Observed band size: 27 kDa
Exposure time: 24.3 s