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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab118776-10μl |
10μl |
期货  |
| |
| Ab118776-20μl |
20μl |
期货 |
| |
| Ab118776-50μl |
50μL |
现货 |
| |
| Ab118776-100μl |
100μL |
现货 |
| |
| Ab118776-1ml |
1ml |
期货 |
|
| 产品名称 | CD8 alpha Mouse mAb |
|---|---|
| 英文别名 | 2-phospho-D-glycerate hydro-lyase | EC 4.2.1.11 | Enolase 2 | Neural enolase | Neuron-specific enolase | NSE |
| 规格或纯度 | mAb (C14); Mouse anti Human CD8 alpha Antibody; WB, IHC; Unconjugated |
| 特异性 | NSE |
| 交叉反应 | Mouse, Rat |
| 免疫原 | Recombinant NSE expressed in E.coli (AA 2-285) |
| 阳性对照 | WB: Hep G2, NIH/3T3, C6 and Jurkat cell lysates. IHC: Human Glioma Tissue, Human Cerebrum Tissue. IF/ICC: Hep G2 cells. |
| 偶联标记 | 1 |
| 克隆 | 2 |
|---|---|
| 克隆号 | C10 |
| Format | Whole IgG |
| 亚型 | Mouse IgG2b |
| 轻链亚型 | Kappa |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Protein A + Protein G purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4 |
| 防腐剂 | 0.02% Sodium azide |
| 浓度 | Lot by Lot |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| ICC/IF |
5-30 µg/mL
|
| WB | 0.5-2 µg/mL |
| IHC | 5-30 µg/mL |
输入批号以搜索COA:
NSE Mouse mAb (Ab118776) - Western Blot
All lanes: NSE Mouse mAb (Ab118776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 45 kDa
Exposure time: 16.4 s
NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Glioma Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IHC
DAB staining on IHC
Samples: Human Cerebrum Tissue
Primary Ab: 30µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
NSE Mouse mAb (Ab118776) - IF/ICC
FITC staining on IF
Samples: Human Hep G2 cells
Primary Ab: 20µg/mL NSE Mouse mAb (Ab118776)
Second Ab: 1µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
| 1. He Huang,Shuang Tang,Ming Ji,Zhanyun Tang,Miho Shimada,Xiaojing Liu,Shankang Qi,Jason W Locasale,Robert G Roeder,Yingming Zhao,Xiaoling Li. (2018-05-19) p300-Mediated Lysine 2-Hydroxyisobutyrylation Regulates Glycolysis.. Molecular cell, 70 ((4)): (663-678). [PMID:29775581] |
| 2. Oliva, D D, Calì, L L, Feo, S S and Giallongo, A A.. (1991) Complete structure of the human gene encoding neuron-specific enolase.. Genomics, (): (). [PMID:2045099] |
| 3. Oliva, D D, Barba, G G, Barbieri, G G, Giallongo, A A and Feo, S S.. (1989) Cloning, expression and sequence homologies of cDNA for human gamma enolase.. Gene, (15): (). [PMID:2792767] |
| 4. McAleese, S M SM, Dunbar, B B, Fothergill, J E JE, Hinks, L J LJ and Day, I N IN.. (1988) Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE).. European journal of biochemistry, (15): (). [PMID:3208766] |
| 5. Van Obberghen, E E and 5 more authors.. (1988) Human gamma enolase: isolation of a cDNA clone and expression in normal and tumor tissues of human origin.. Journal of neuroscience research, (): (). [PMID:3385803] |
| 6. Day, I N IN, Allsopp, M T MT, Moore, D C DC and Thompson, R J RJ.. (1987) Sequence conservation in the 3'-untranslated regions of neurone-specific enolase, lymphokine and protooncogene mRNAs.. FEBS letters, (28): (). [PMID:3653393] |
| 7. Ansari-Lari, M A MA, Shen, Y Y, Muzny, D M DM, Lee, W W and Gibbs, R A RA.. (1997) Large-scale sequencing in human chromosome 12p13: experimental and computational gene structure determination.. Genome research, (): (). [PMID:9074930] |
| 8. Chai, Geqing G and 5 more authors.. (2004) Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase.. Journal of molecular biology, (20): (). [PMID:15289101] |
| 9. Rush, John J and 9 more authors.. (2005) Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.. Nature biotechnology, (): (). [PMID:15592455] |
| 10. Scherer, Steven E SE and 216 more authors.. (2006) The finished DNA sequence of human chromosome 12.. Nature, (16): (). [PMID:16541075] |
| 11. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): (). [PMID:23186163] |
| 12. Leonard, Paul G PG and 17 more authors.. (2016) SF2312 is a natural phosphonate inhibitor of enolase.. Nature chemical biology, (): (). [PMID:27723749] |
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