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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab122143-10μl |
10μl |
期货  |
| |
| Ab122143-20μl |
20μl |
期货 |
| |
| Ab122143-50μl |
50μL |
现货 |
| |
| Ab122143-100μl |
100μL |
现货 |
| |
| Ab122143-1ml |
1ml |
期货 |
|
| 产品名称 | PKM Antibody |
|---|---|
| 别名 | M2型丙酮酸激酶多克隆抗体 |
| 英文别名 | CTHBP antibody | Cytosolic thyroid hormone-binding protein antibody | KPYM_HUMAN antibody | OIP-3 antibody | Opa-interacting protein 3 antibody | p58 antibody | pkm antibody | PKM1 antibody | PKM2 antibody | Pyruvate kinase 2/3 antibody | Pyruvate kinase |
| 规格或纯度 | pAb; Rabbit anti Human PKM Antibody; WB, IHC, ICC, IF; Unconjugated |
| 特异性 | PKM |
| 免疫原 | Recombinant PKM2 expressed in E.coli (AA 141-248) |
| 阳性对照 | WB: K562, MCF7, HeLa, HepG2, A431, THP-1, Raji, NIH/3T3 and C6 cell lysates. IHC: Human breast cancer tissue; Human skin cancer tissue, Human prostate tissue, Human prostate cancer tissue, Human colorectal cancer tissue, Human liver cancer tissue. ICC/IF |
| 偶联标记 | 1 |
| 克隆 | 3 |
|---|---|
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified and Protein A purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 10 mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4 |
| 防腐剂 | 0.05% Proclin 300 |
| 浓度 | Lot by Lot |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| IHC | 5-20 µg/mL |
| ICC/IF |
5-20 µg/mL
|
| WB |
0.01-2 µg/mL
|
输入批号以搜索COA:
PKM Antibody (Ab122143) - Western Blot
All lanes: PKM Antibody (Ab122143) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 1.6 s
PKM Antibody (Ab122143) - IF/ICC
FITC staining on IF
Samples: Human MCF7 cells
Primary Ab: 20 ug/mL PKM Antibody (Ab122143)
Second Ab: 1 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Breast cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Skin cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Prostate cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Colorectal cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
PKM Antibody (Ab122143) - IHC
DAB staining on IHC
Samples: Human Liver cancer Tissue
Primary Ab: 10 µg/mL PKM Antibody (Ab122143)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
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