Recombinant PP2A alpha/beta Antibody

功能和特点

规格或纯度: ExactAb™, Validated, Recombinant, 0.2 mg/mL
简短描述: Recombinant (R04-4G4); Rabbit anti Human PP2A alpha/beta Antibody; WB, IHC, ICC, IF; Unconjugated
反应种属: Hamster ,Human,Mouse ,Rat
亚型: Rabbit IgG

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库存信息

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货号 (SKU)	包装规格	是否现货
Ab122709-10μl	10μl	期货
Ab122709-50μl	50μL	现货
Ab122709-100μl	100μL	现货
Ab122709-1ml	1ml	期货

查看相关系列

Accession#:P62714 Accession#:P67775 Gene ID:5515 Gene ID:5516 PP2A alpha/beta PPP2CA PPP2CB

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基本信息

产品名称	Recombinant PP2A alpha/beta Antibody
别名	PPP2CA; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; Replication protein C; RP-C; PP2A A antibody; PP2A alpha antibody; PP2A B antibody; PP2A beta antibody; PP2A-alpha antibody; PP2A-beta antibody; PP2AA_HUMAN antib
规格或纯度	ExactAb™, Validated, Recombinant, 0.2 mg/mL
特异性	PPP2CA/PPP2CB
反应种属	Hamster ,Human,Mouse ,Rat
免疫原	A synthetic peptide derived from human PP2A alpha/beta (AA 277-294).
阳性对照	WB: HeLa, C6, NIH/3T3, A431, Jurkat, HEK293, A549 and Raw264.7 cell lysates. IHC: Human lung cancer. ICC/IF: HeLa cells.
偶联标记	Unconjugated

产品属性

克隆	Recombinant
克隆号	R04-4G4
Format	Whole IgG
亚型	Rabbit IgG
SDS-PAGE	150 kDa
纯化方法	Immunogen affinity purified
物理外观	Liquid
储存缓冲液	50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4.
防腐剂	0.01% Sodium azide
浓度	0.2 mg/mL
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application	Notes
WB	1/500-1/1000
IHC	1/50 - 1/100
ICC/IF	1/50-1/200

质检证书(COA)

质检报告(COA)

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图片

Western Blot: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - Western Blot
All lanes: Recombinant PP2A alpha/beta Antibody (Ab122709) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 36 kDa
Observed band size: 34 kDa
Exposure time: 10.0 s

IF: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - IF
Immunocytochemistry analysis of PP2A alpha/beta (green) in HeLa cells using Recombinant PP2A alpha/beta Antibody (Ab122709) and cells were counterstained with DAPI (blue).

IHC: Recombinant PP2A alpha/beta Antibody (Ab122709)

Recombinant PP2A alpha/beta Antibody (Ab122709) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant PP2A alpha/beta Antibody (Ab122709). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.