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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab126009-10μl |
10μl |
期货  |
| |
| Ab126009-50μl |
50μL |
现货 |
| |
| Ab126009-100μl |
100μL |
现货 |
| |
| Ab126009-1ml |
1ml |
期货 |
|
| 产品名称 | Recombinant RRM1 Antibody |
|---|---|
| 别名 | 核苷酸还原酶M1重组兔单克隆抗体 |
| 英文别名 | R1 antibody | Ribonucleoside diphosphate reductase large subunit antibody | Ribonucleoside diphosphate reductase M1 chain antibody | Ribonucleoside diphosphate reductase subunit M1 antibody | Ribonucleoside reductase, large subunit antibody | Ribonucleosi |
| 规格或纯度 | Recombinant (R08-4D3); Rabbit anti Human RRM1 Antibody; WB, IHC, ICC, IF; Unconjugated |
| 特异性 | RRM1 |
| 免疫原 | A synthetic peptide derived from human RRM1 (AA 732-745) |
| 阳性对照 | WB: C6, NIH/3T3, HeLa, A549, HT-29, HepG2 and MCF-7 cell lysates. IHC: Human lung cancer tissue. ICC/IF: HeLa cells. |
| 偶联标记 | 1 |
| 克隆 | 1 |
|---|---|
| 克隆号 | R08-4D3 |
| Format | Whole IgG |
| 亚型 | Rabbit IgG |
| SDS-PAGE | 150 kDa |
| 纯化方法 | Antigen affinity purified |
| 物理外观 | Liquid |
| 储存缓冲液 | 40% Glycerol, 150 mM NaCl, 50 mM Tris-Glycine, 0.05% BSA, 0.01% Sodium azide, pH 7.4. |
| 防腐剂 | 0.01% Sodium azide |
| 浓度 | 1.0 mg/mL |
| 储存温度 | -20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle. |
| Application | Notes |
|---|---|
| IHC | 1/50 - 1/100 |
| ICC/IF |
1/50-1/200
|
| WB |
1/500-1/1000
|
输入批号以搜索COA:
Recombinant RRM1 Antibody (Ab126009) - Western Blot
All lanes: Recombinant RRM1 Antibody (Ab126009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 86 kDa
Exposure time: 1.8 s
Recombinant RRM1 Antibody (Ab126009) - Western Blot
All lanes: Recombinant RRM1 Antibody (Ab126009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 86 kDa
Exposure time: 7.4 s
Recombinant RRM1 Antibody (Ab126009) - IF/ICC
Immunocytochemistry analysis of RRM1 (Ab126009) (green) in HeLa using Recombinant RRM1 Antibody (Ab126009), and cells were counterstained with DAPI (blue).
Recombinant RRM1 Antibody (Ab126009) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant RRM1 Antibody (Ab126009) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
| 1. Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M. (2009) Lysine acetylation targets protein complexes and co-regulates major cellular functions.. Science, 325 (5942): (834-40). [PMID:19608861] |
| 2. Tan L, Gurbani D, Weisberg EL, Jones DS, Rao S, Singer WD, Bernard FM, Mowafy S, Jenney A, Du G et al.. (2017) Studies of TAK1-centered polypharmacology with novel covalent TAK1 inhibitors.. Bioorg Med Chem, 25 (4): (1320-1328). [PMID:28038940] |
| 3. Ahmad MF, Huff SE, Pink J, Alam I, Zhang A, Perry K, Harris ME, Misko T, Porwal SK, Oleinick NL, Miyagi M, Viswanathan R, Dealwis CG.. (2015) Identification of Non-nucleoside Human Ribonucleotide Reductase Modulators.. J Med Chem, 58 (24): (9498-9509). [PMID:26488902] |
| 4. Huff SE, Mohammed FA, Yang M, Agrawal P, Pink J, Harris ME, Dealwis CG, Viswanathan R.. (2018) Structure-Guided Synthesis and Mechanistic Studies Reveal Sweetspots on Naphthyl Salicyl Hydrazone Scaffold as Non-Nucleosidic Competitive, Reversible Inhibitors of Human Ribonucleotide Reductase.. J Med Chem, 61 (3): (666-680). [PMID:29253340] |
| 5. Pavloff, N N, Rivard, D D, Masson, S S, Shen, S H SH and Mes-Masson, A M AM.. (1992) Sequence analysis of the large and small subunits of human ribonucleotide reductase.. DNA sequence : the journal of DNA sequencing and mapping, (): (). [PMID:1627826] |
| 6. Parker, N J NJ, Begley, C G CG and Fox, R M RM.. (1991) Human M1 subunit of ribonucleotide reductase: cDNA sequence and expression in stimulated lymphocytes.. Nucleic acids research, (11): (). [PMID:1840662] |
| 7. Parker, N J NJ, Begley, C G CG and Fox, R M RM.. (1994) Human R1 subunit of ribonucleotide reductase (RRM1): 5' flanking region of the gene.. Genomics, (1): (). [PMID:8188248] |
| 8. Takahashi, T T, Kanazawa, J J, Akinaga, S S, Tamaoki, T T and Okabe, M M.. (1999) Antitumor activity of 2-chloro-9-(2-deoxy-2-fluoro-beta-D-arabinofuranosyl) adenine, a novel deoxyadenosine analog, against human colon tumor xenografts by oral administration.. Cancer chemotherapy and pharmacology, (): (). [PMID:9923554] |
| 9. Bepler, G G, O'briant, K C KC, Kim, Y C YC, Schreiber, G G and Pitterle, D M DM.. (1999) A 1.4-Mb high-resolution physical map and contig of chromosome segment 11p15.5 and genes in the LOH11A metastasis suppressor region.. Genomics, (15): (). [PMID:9933563] |
| 10. Skierski, J S JS, Koronkiewicz, M M and Grieb, P P.. (1999) Effect of FMdC on the cell cycle of some leukemia cell lines.. Cytometry, (1): (). [PMID:10547615] |
| 11. Xue, Lijun L and 5 more authors.. (2003) Wild-type p53 regulates human ribonucleotide reductase by protein-protein interaction with p53R2 as well as hRRM2 subunits.. Cancer research, (1): (). [PMID:12615712] |
| 12. Zhou, Bingsen B and 9 more authors.. (2003) The human ribonucleotide reductase subunit hRRM2 complements p53R2 in response to UV-induced DNA repair in cells with mutant p53.. Cancer research, (15): (). [PMID:14583450] |
| 13. Qiu, Weihua W, Zhou, Bingsen B, Darwish, Dana D, Shao, Jimin J and Yen, Yun Y.. (2006) Characterization of enzymatic properties of human ribonucleotide reductase holoenzyme reconstituted in vitro from hRRM1, hRRM2, and p53R2 subunits.. Biochemical and biophysical research communications, (10): (). [PMID:16376858] |
| 14. Rosell, R R, Cobo, M M, Isla, D D, Camps, C C and Massuti, B B.. (2006) Pharmacogenomics and gemcitabine.. Annals of oncology : official journal of the European Society for Medical Oncology, (): (). [PMID:16807441] |
| 15. Bepler, Gerold G and 6 more authors.. (2006) RRM1 modulated in vitro and in vivo efficacy of gemcitabine and platinum in non-small-cell lung cancer.. Journal of clinical oncology : official journal of the American Society of Clinical Oncology, (10): (). [PMID:16966686] |
| 16. Smid, K K and 7 more authors.. (2006) Micro-array analysis of resistance for gemcitabine results in increased expression of ribonucleotide reductase subunits.. Nucleosides, nucleotides & nucleic acids, (): (). [PMID:17065054] |
| 17. Nakahira, Shin S and 15 more authors.. (2007) Involvement of ribonucleotide reductase M1 subunit overexpression in gemcitabine resistance of human pancreatic cancer.. International journal of cancer, (15): (). [PMID:17131328] |
| 18. Harned, Theresa M and Gaynon, Paul S.. (2008) Treating refractory leukemias in childhood, role of clofarabine.. Therapeutics and clinical risk management, (): (). [PMID:18728851] |
| 19. Robak, Tadeusz; Korycka, Anna; Lech-Maranda, Ewa and Robak, Pawel.. (2009) Current status of older and new purine nucleoside analogues in the treatment of lymphoproliferative diseases.. Molecules (Basel, Switzerland), (23): (). [PMID:19325518] |
| 20. Pession, A; Masetti, R; Kleinschmidt, K and Martoni, A.. (2010) Use of clofarabine for acute childhood leukemia.. Biologics : targets & therapy, (24): (). [PMID:20631817] |
| 21. Fairman, James Wesley JW and 13 more authors.. (2011) Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization.. Nature structural & molecular biology, (): (). [PMID:21336276] |
| 22. Arnaoutov, Alexei A and Dasso, Mary M.. (2014) Enzyme regulation. IRBIT is a novel regulator of ribonucleotide reductase in higher eukaryotes.. Science (New York, N.Y.), (19): (). [PMID:25237103] |
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