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Recombinant Smad4 Antibody

功能和特点
  • 规格或纯度: Recombinant (R05-1I7); Rabbit anti Human Smad4 Antibody; WB, ICC, IF; Unconjugated
  • 简短描述: Rabbit recombinant (R05-1I7) to Smad4
  • 反应种属:
  • 亚型: Rabbit IgG
有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
Ab128223-10μl
10μl 期货 Stock Image
Ab128223-50μl
50μL 现货 Stock Image
Ab128223-100μl
100μL 现货 Stock Image
Ab128223-1ml
1ml 现货 Stock Image

基本信息

产品名称 Recombinant Smad4 Antibody
英文别名 Deletion target in pancreatic carcinoma 4 | hSMAD4 | MAD homolog 4 | Mothers against DPP homolog 4 | SMAD 4 | SMAD family member 4 | Smad4
规格或纯度 Recombinant (R05-1I7); Rabbit anti Human Smad4 Antibody; WB, ICC, IF; Unconjugated
特异性 SMAD4
免疫原 A synthetic peptide derived from human Smad4 (AA 316-552).
阳性对照 WB: NIH/3T3, C6, Ramos ,HUVEC and A549 cell lysates. ICC/IF: HeLa cells.
偶联标记 1

产品属性

克隆 1
克隆号 R05-1I7
Format Whole IgG
亚型 Rabbit IgG
SDS-PAGE 150 kDa
纯化方法 Immunogen affinity purified
物理外观 Liquid
储存缓冲液 50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂 0.01% Sodium azide
浓度 0.6 mg/mL
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

应用

Application Notes
WB

1/500-1/1000

ICC/IF

1/50-1/200

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图片

Recombinant Smad4 Antibody (Ab128223) - Western Blot
All lanes: Recombinant Smad4 Antibody (Ab128223) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 60 kDa
Observed band size: 63 kDa
Exposure time: 4.3 s

Recombinant Smad4 Antibody (Ab128223) - IF/ICC
Immunocytochemistry analysis of Smad4 (green) in HeLa cells using Recombinant Smad4 Antibody (Ab128223), and cells were counterstained with DAPI (blue).

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参考文献

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