This lectin has a carbohydrate specificity toward α-linked N-acetylgalactosamine. It has been used to establish secretor status in blood group A individuals by hemagglutination inhibition techniques and for blood typing. This lectin has also been used as a general marker of developing renal collecting ducts.
Dolichos biflorus agglutinin is a glycoprotein with a molecular weight of about 111 kDa and consists of 4 subunits of approximately equal size.
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
Specifications
Molecular Weight
111 kDa
Extinction Coefficient
1.22
Inhibiting or Eluting Sugar
GalNAc
Storage Instructions
2-8 ℃
Sugar Specificity
Forssman antigen and GM2
Usage Summary
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl₂ is recommended. For preserving solutions stored at 4 ℃, 0.08% sodium azide can be used. DO NOT VORTEX!
Reconstitute in 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. DO NOT VORTEX!