计算溶液所需的质量、体积或浓度。
刺桐凝集素 (ECL)
有货
为什么选择此级别
BioReagent,天然 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
The carbohydrate structure to which ECL binds is frequently found in membrane and serum glycoproteins of mammalian origin. Sialic acid substitution on this structure appears to prevent the lectin from binding. This specificity offers an opportunity to utilize agarose bound ECL to isolate or fractionate mammalian glycoproteins.
This lectin has been reported to be useful for the isolation of human natural killer (NK) cells using a negative selection panning technique (protocol is provided below). Human NK cells appear to lack accessible surface carbohydrate structures required for binding ECL and, unlike other mononuclear cells, do not adhere to ECL-coated culture dishes. Since this procedure involves a negative selection panning technique, a high recovery of viable NK cells can be obtained. The adherent cells can also be recovered by incubation in galactose or lactose.
Erythrina cristagalli lectin consists of two different subunits of approximately 28 kDa and 26 kDa.
Inhibiting/Eluting Sugar: 200 mM lactose
Specifications
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User Guide: Enrichment of NK Cells by Panning on Erythrina Cristagalli Plates
This procedure is a modification of the method for NK cell isolation described in the following reference: Koren HS, et al. 1985. Novel Approaches for Enrichment of NK Cells. Macrophage Biology. A variety of methods are used to obtain enriched fractions of NK cells, such as column adherence techniques, Percoll discontinuous gradients, and monoclonal antibodies. The following procedure utilizes the lectin isolated from Erythrina Cristagalli Lectin, taking advantage of its ability to discriminate between NK cells and other mononuclear cells in a negative selection technique.
Procedure:
1. Bacteriological plastic plates (60 mm) are coated with 2 ml of Erythrina Cristagalli lectin in PBS, pH 7.8, at a concentration of 250 μg/ml for 1 h at 24°C.
2. Decant the unbound Erythrina Cristagalli lectin and wash the plate thoroughly with PBS. The decanted lectin solution may be stored at 4°C with 0.05% sodium azide and reused for further plating.
3. Add 1 x 107 lymphocytes in 4 ml of RPMI-1640 or PBS for 1 h at 4°C.
4. After collecting the unbound cells (enriched NK fraction), the bound cells can be removed by the addition of 200 mM lactose, 0.5 mM EDTA.
5. Both the unbound and bound cells can be washed several times with RPMI or PBS containing 200 mM lactose to remove any trace amounts of lectin which might be present.
规格
产品名称
刺桐凝集素 (ECL)
英文别名
Lectin from Erythrina Cristagalli | ECA
规格或纯度
BioReagent, 天然
种属
Erythrina Cristagalli
偶联
Unconjugated
来源
天然
预测分子量
54 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
复溶
Reconstitute in 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH 8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
储存条件
2-8°C储存
运输条件
低温运输
稳定性与储存
长期储存2-8℃；复溶后建议分装。
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0737609 |
分析证书 | E1452704 |
| ZJ26F0737608 |
分析证书 | E1452704 |
技术文档和文章
溶液计算器
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