GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine
Specifications
Molecular Weight
114 kDa
Extinction Coefficient
1.4
Inhibiting or Eluting Sugar
Raffinose
Storage Instructions
2–8 °C
Sugar Specificity
Terminal αGal and terminal αGalNAc
Usage Summary
Although many buffers can be employed for reconstituting this lectin, we recommend 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH 8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. For dissolving the lectin, we recommend adding the lectin slowly to buffer rather than adding the buffer to the lectin.Allow the lectin to dissolve without vigorous agitation.
Griffonia (Bandeiraea) Simplicifolia Lectin I | BSL I | BS-I | Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) | Bandeirea simplicifolia agglutinin I | BSI
规格或纯度
BioReagent, 天然
偶联
Unconjugated
预测分子量
114 kDa
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
复溶
Reconstitute in 10 mM HEPES buffered saline, 0.1 mM CaCl₂, pH 8.5. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. For dissolving the lectin, we recommend adding the lectin slowly to buffer rather than adding the buffer to the lectin. Allow the lectin to dissolve without vigorous agitation.